Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,564 C→G 37.6% R43R (CGC→CGG RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,5640C→G37.6% 0.0 ‑0.5 ‑0.8481R43R (CGC→CGGRCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (93/88):  ref base (162/138):  total (255/226)
Fisher's exact test strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.76e-01

Only 100 of 521 total aligned reads displayed.

TCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGA  >  NC_014034/2155464‑2155664
                                                                                                    |                                                                                                     
 cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt                                                                                                      >  2:2580038/1‑101 (MQ=255)
 cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt                                                                                                      >  2:4278592/1‑101 (MQ=255)
 cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt                                                                                                      <  2:3555017/101‑1 (MQ=255)
  gtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCtt                                                                                                     <  1:3555278/101‑1 (MQ=255)
    cgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtttt                                                                                                   >  1:3497403/1‑99 (MQ=255)
    cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc                                                                                                   >  2:4089725/1‑101 (MQ=255)
    cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc                                                                                                   >  1:1514289/1‑101 (MQ=255)
     gATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGttttt                                                                                                  >  1:1235451/1‑98 (MQ=255)
      tttccctCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.g                                                                                                <  1:2282277/95‑1 (MQ=255)
        gCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAg                                                                                              >  2:2751701/1‑101 (MQ=255)
         ccATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGc                                                                                             >  2:4572452/1‑101 (MQ=255)
          cATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GGGcc                                                                                            >  2:6856805/1‑101 (MQ=255)
          cATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGcc                                                                                            >  2:4717511/1‑101 (MQ=255)
             cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCcgcg                                                                                         >  1:4679946/1‑101 (MQ=255)
               ggggcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGGGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAg                                                                                       >  1:3637730/1‑101 (MQ=255)
                   cggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAgctgc                                                                                   <  2:2464652/101‑1 (MQ=255)
                   cggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAgctgc                                                                                   >  1:759340/1‑101 (MQ=255)
                      ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGctct                                                                                >  2:1265593/1‑101 (MQ=255)
                      ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCGTGGGCGCAAGCGCCGCGTCCT.GGGCCGGGATGTGctcg                                                                                >  1:545766/1‑100 (MQ=255)
                       gcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTg                                                                               <  2:417138/101‑1 (MQ=255)
                         cGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTggg                                                                             <  1:566616/101‑1 (MQ=255)
                         cGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGAAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTggg                                                                             <  1:618096/101‑1 (MQ=255)
                          gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGa                                                                            >  2:703188/1‑101 (MQ=255)
                          gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGa                                                                            >  1:285997/1‑101 (MQ=255)
                            cacgacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGc                                                                          >  1:2352434/1‑101 (MQ=255)
                            cacgaagATGGCGGCAGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGAG.CGTGAACTGCTGTGGGAGc                                                                          >  1:6853539/7‑101 (MQ=255)
                            caccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGc                                                                          >  2:5522881/1‑101 (MQ=255)
                             acgacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCt                                                                         >  2:308356/4‑101 (MQ=255)
                             accacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCt                                                                         >  1:1596810/1‑101 (MQ=255)
                               gacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGc                                                                       >  2:6050237/2‑101 (MQ=255)
                                 cGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGccg                                                                     >  2:5893051/1‑100 (MQ=255)
                                  gATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCg                                                                    >  1:2626968/1‑101 (MQ=255)
                                   aTGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGccggg                                                                   >  2:2006020/1‑98 (MQ=255)
                                    tGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTc                                                                  <  2:5075804/101‑1 (MQ=255)
                                    gggcggcggcGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTc                                                                  <  2:5281524/100‑1 (MQ=255)
                                       cggcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTggcc                                                               >  2:193846/1‑100 (MQ=255)
                                        ggcggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCgg                                                              >  2:897424/1‑101 (MQ=255)
                                         gcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGt                                                             <  1:1820466/101‑1 (MQ=255)
                                         gcggcgCGCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGt                                                             <  2:6328624/101‑1 (MQ=255)
                                           ggcgCTCGCCGGCCGGCTGATGTGGCATACGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCg                                                           >  1:5056203/1‑101 (MQ=255)
                                              gcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCa                                                        >  1:319491/1‑101 (MQ=255)
                                              gcTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCa                                                        >  1:3130477/1‑101 (MQ=255)
                                               cTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCAt                                                       <  2:82082/101‑1 (MQ=255)
                                               cTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCAt                                                       <  2:1504569/101‑1 (MQ=255)
                                                 cGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCACAAGCGCCGCTTCCT.TAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATgg                                                     >  1:4052394/1‑101 (MQ=255)
                                                  gCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGc                                                    >  1:5200901/1‑101 (MQ=255)
                                                   ccGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCg                                                   <  1:1854674/101‑1 (MQ=255)
                                                   ccGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCg                                                   <  1:6729211/101‑1 (MQ=255)
                                                    cGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGa                                                  >  1:4875271/1‑101 (MQ=255)
                                                     gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                 >  1:861248/1‑101 (MQ=255)
                                                     gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                 <  1:7011936/101‑1 (MQ=255)
                                                     gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                 >  1:6552984/1‑101 (MQ=255)
                                                      ggcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatc                                                <  2:3204596/101‑1 (MQ=255)
                                                       gcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatca                                               >  1:3765008/1‑101 (MQ=255)
                                                       gcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatca                                               >  2:2218706/1‑101 (MQ=255)
                                                       gcgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatca                                               <  1:5578289/101‑1 (MQ=255)
                                                        cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                              <  1:939216/101‑1 (MQ=255)
                                                        cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                              <  1:1392882/101‑1 (MQ=255)
                                                         ggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcatc                                             >  1:1043055/1‑101 (MQ=255)
                                                         ggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcatc                                             >  1:422740/1‑101 (MQ=255)
                                                         ggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcatc                                             <  1:4469896/101‑1 (MQ=255)
                                                         gccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcatc                                             <  2:1747622/99‑1 (MQ=255)
                                                            tGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGc                                          >  1:1003065/1‑101 (MQ=255)
                                                            tGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGc                                          >  1:2960588/1‑101 (MQ=255)
                                                            tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                          >  2:1608421/1‑101 (MQ=255)
                                                            tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                          >  1:5921328/1‑101 (MQ=255)
                                                            tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                          <  1:3870882/101‑1 (MQ=255)
                                                            tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                          >  2:5761108/1‑101 (MQ=255)
                                                             gATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                         >  2:3698895/1‑101 (MQ=255)
                                                             gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                         >  2:1152342/1‑101 (MQ=255)
                                                             gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                         <  1:4572962/101‑1 (MQ=255)
                                                              aTGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGa                                        >  2:4034873/1‑101 (MQ=255)
                                                              aTGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGa                                        <  2:2932418/101‑1 (MQ=255)
                                                               tgtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAg                                       >  2:2129728/1‑101 (MQ=255)
                                                               tgtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAg                                       <  2:4354492/101‑1 (MQ=255)
                                                               tgtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAg                                       <  1:6264845/101‑1 (MQ=255)
                                                                 tgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGc                                     <  2:6412365/101‑1 (MQ=255)
                                                                 tgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGc                                     <  1:4956665/101‑1 (MQ=255)
                                                                 tgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGCCGCGGTCGGCATGGCGATCATCGGCGAGGc                                     <  1:1109114/101‑1 (MQ=255)
                                                                   gCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCgg                                   <  2:2313397/101‑1 (MQ=255)
                                                                    cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                  <  1:4988506/101‑1 (MQ=255)
                                                                    cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                  <  2:1022601/101‑1 (MQ=255)
                                                                    cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCGGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                  <  2:3924416/101‑1 (MQ=255)
                                                                     aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGATg                                 <  1:2568314/101‑1 (MQ=255)
                                                                        cGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcg                              >  2:6736999/1‑101 (MQ=255)
                                                                        cGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcg                              <  1:2092076/101‑1 (MQ=255)
                                                                         gACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcgg                             >  1:6070963/1‑101 (MQ=255)
                                                                          aCCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcggc                            >  2:3807647/1‑101 (MQ=255)
                                                                            cGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                          <  2:6583550/101‑1 (MQ=255)
                                                                            cGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                          <  2:6697084/101‑1 (MQ=255)
                                                                             gAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                         <  1:5371024/101‑1 (MQ=255)
                                                                             gAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGGTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                         >  2:3462029/1‑101 (MQ=255)
                                                                                  gCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCg                    <  1:663336/101‑1 (MQ=255)
                                                                                    gggCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAg                  <  2:4987328/101‑1 (MQ=255)
                                                                                     ggCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGc                 >  1:5774020/1‑101 (MQ=255)
                                                                                      gcgcgcAAGCGCCGCTGCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCt                <  2:4875027/101‑1 (MQ=255)
                                                                                          gcAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGc            >  1:1324730/1‑101 (MQ=255)
                                                                                               cgccgcTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAc       <  2:744055/101‑1 (MQ=255)
                                                                                                   gcTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTg   <  1:6425827/101‑1 (MQ=255)
                                                                                                   gcTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTg   <  2:2040196/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
TCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGA  >  NC_014034/2155464‑2155664

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG