Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,585 C→G 41.0% L50L (CTC→CTG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,5850C→G41.0% 0.0 ‑2.0 ‑0.0526L50L (CTC→CTG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (119/97):  ref base (150/160):  total (269/257)
Fisher's exact test strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.53e-01

Only 100 of 550 total aligned reads displayed.

GGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGC  >  NC_014034/2155485‑2155685
                                                                                                     |                                                                                                    
 ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCGTGGGCGCAAGCGCCGCGTCCT.GGGCCGGGATGTGctcg                                                                                                     >  1:545766/1‑100 (MQ=255)
     gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGa                                                                                                 >  1:285997/1‑101 (MQ=255)
       cacgaagATGGCGGCAGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGAG.CGTGAACTGCTGTGGGAGc                                                                                               >  1:6853539/7‑101 (MQ=255)
       caccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGc                                                                                               >  2:6425497/1‑101 (MQ=255)
       caccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGc                                                                                               <  1:2201993/101‑1 (MQ=255)
             gATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGccgg                                                                                         >  1:5788196/1‑99 (MQ=255)
             gATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGccgg                                                                                         >  1:3768448/1‑99 (MQ=255)
             gATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCg                                                                                         >  1:466052/1‑101 (MQ=255)
              aTGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGt                                                                                        >  2:6731464/1‑101 (MQ=255)
               tGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTc                                                                                       >  2:4038381/1‑101 (MQ=255)
                ggcggcggcGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGtgg                                                                                      <  2:6755826/101‑3 (MQ=255)
                  cggcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTggcc                                                                                    <  2:580120/101‑2 (MQ=255)
                  cggcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTggcc                                                                                    >  2:193846/1‑100 (MQ=255)
                  cggcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTggcc                                                                                    <  1:3682091/101‑2 (MQ=255)
                     cggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTc                                                                                 <  1:2751803/101‑1 (MQ=255)
                      ggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGtgg                                                                                >  1:6230275/1‑99 (MQ=255)
                      ggcgCTCGCCGGCCGGCTGATGTGGCATACGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCg                                                                                >  1:5056203/1‑101 (MQ=255)
                        cgcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGc                                                                              <  2:1520993/101‑1 (MQ=255)
                        cgcTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGc                                                                              >  1:4894177/1‑101 (MQ=255)
                        cgcTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGc                                                                              <  2:5002401/101‑1 (MQ=255)
                         gcTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCa                                                                             >  1:3130477/1‑101 (MQ=255)
                          cTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCAt                                                                            <  2:82082/101‑1 (MQ=255)
                           tCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATg                                                                           <  2:454238/101‑1 (MQ=255)
                            cGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATgg                                                                          <  1:6530499/101‑1 (MQ=255)
                             gCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGc                                                                         >  1:5200901/1‑101 (MQ=255)
                              ccGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCg                                                                        <  2:6647973/101‑1 (MQ=255)
                               cGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGa                                                                       >  1:2591927/1‑101 (MQ=255)
                                gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                                      >  1:861248/1‑101 (MQ=255)
                                 ggcgccTGATGTGGCATTCGCCCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCCatc                                                                     <  1:5613722/101‑1 (MQ=255)
                                  gcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatca                                                                    >  2:1121162/1‑101 (MQ=255)
                                   cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                                                   <  2:4640363/101‑1 (MQ=255)
                                   cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                                                   <  1:6145006/101‑1 (MQ=255)
                                   cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                                                   <  1:6975689/98‑1 (MQ=255)
                                   cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                                                   <  2:2387615/98‑1 (MQ=255)
                                   cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                                                   <  1:1374033/98‑1 (MQ=255)
                                   cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                                                   <  2:2017540/98‑1 (MQ=255)
                                    ggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcatc                                                                  <  1:4469896/101‑1 (MQ=255)
                                    ggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcatc                                                                  >  1:1043055/1‑101 (MQ=255)
                                    gccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcatc                                                                  <  2:1747622/99‑1 (MQ=255)
                                     ccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCg                                                                 <  1:1827306/100‑1 (MQ=255)
                                      cTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCgg                                                                <  1:1577066/101‑1 (MQ=255)
                                       tGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGc                                                               <  2:1652930/101‑1 (MQ=255)
                                       tGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGc                                                               >  1:1003065/1‑101 (MQ=255)
                                       tGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGc                                                               >  1:2438426/1‑101 (MQ=255)
                                       tGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGc                                                               >  1:2960588/1‑101 (MQ=255)
                                       tGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGc                                                               >  1:5921328/1‑101 (MQ=255)
                                        gATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                                              >  2:3698895/1‑101 (MQ=255)
                                        gATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                                              <  1:1451625/101‑1 (MQ=255)
                                        gATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                                              <  1:388800/101‑1 (MQ=255)
                                          tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                                                            <  2:4893933/101‑1 (MQ=255)
                                           gtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAgg                                                           >  2:4138658/1‑101 (MQ=255)
                                           gtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTCCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAgg                                                           <  2:3356305/101‑1 (MQ=255)
                                            tgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGc                                                          <  2:4089043/101‑1 (MQ=255)
                                            tgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGc                                                          <  1:4956665/101‑1 (MQ=255)
                                             ggCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCg                                                         <  2:2152132/101‑1 (MQ=255)
                                               cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                                       <  2:319487/101‑1 (MQ=255)
                                               cATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCGGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGt                                                       <  2:3924416/101‑1 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTg                                                      >  1:77330/1‑101 (MQ=255)
                                                aTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTg                                                      <  2:422728/101‑1 (MQ=255)
                                                 ttCGACCGCGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTgg                                                     <  2:1003030/101‑1 (MQ=255)
                                                  tCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggg                                                    >  1:4553579/1‑100 (MQ=255)
                                                  tCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGAGCATGGCGATCATCGGCGAGGCGGTggc                                                    >  2:2469823/1‑101 (MQ=255)
                                                  tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggg                                                    >  2:5374712/1‑100 (MQ=255)
                                                  tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggc                                                    <  1:5677745/101‑1 (MQ=255)
                                                  tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggc                                                    <  2:5117235/101‑1 (MQ=255)
                                                     aCCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcggc                                                 >  2:3807647/1‑101 (MQ=255)
                                                     aCCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcggc                                                 <  1:2109702/101‑1 (MQ=255)
                                                       cGAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                                               <  2:6583550/101‑1 (MQ=255)
                                                        gAGGTGCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                                              <  1:5371024/101‑1 (MQ=255)
                                                        gAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                                              >  2:2109096/1‑101 (MQ=255)
                                                         gggTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAt                                             <  1:4765321/100‑1 (MQ=255)
                                                             gCGGGCGCGGCGGCGCCGGTTTTT.CGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCg                                         <  1:663336/101‑1 (MQ=255)
                                                                ggCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGc                                      <  2:3529846/101‑1 (MQ=255)
                                                                 gcgcgcAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCt                                     <  2:2681659/101‑1 (MQ=255)
                                                                  cgcgcAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTc                                    <  1:1121210/101‑1 (MQ=255)
                                                                     gcAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGc                                 >  2:2256374/1‑101 (MQ=255)
                                                                      cAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGcc                                >  2:7006347/1‑101 (MQ=255)
                                                                        aGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCgg                              <  1:3458654/101‑1 (MQ=255)
                                                                         gcgcCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGa                             <  2:4345210/101‑1 (MQ=255)
                                                                             cgcTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCgg                         >  2:3069183/1‑100 (MQ=255)
                                                                             cgcTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGt                         <  1:1317588/101‑1 (MQ=255)
                                                                                ttCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGac                      >  2:565052/1‑101 (MQ=255)
                                                                                ttCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTCGCGGCTTATTTCGAGCTCGGCCGGACCGTGac                      >  1:3546277/1‑101 (MQ=255)
                                                                                 tCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGacc                     <  1:968422/101‑1 (MQ=255)
                                                                                 tCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGacc                     <  1:4138853/101‑1 (MQ=255)
                                                                                   cT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccac                   >  2:1216398/1‑101 (MQ=255)
                                                                                    t.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGaccacc                  <  1:631098/101‑1 (MQ=255)
                                                                                    t.GAGCCGCGAGCTGCTCTGGGAGCTGCCAGTCGCGGTCGGCATGGGGATCATCGGCGAGGCGGAGGCGGCTTATTTCGAGCTCGGCCGGACCCGGaccacc                  >  1:1724873/1‑101 (MQ=255)
                                                                                      gAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCg                 <  2:7085033/101‑1 (MQ=255)
                                                                                      cgggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCg                 >  2:1351808/11‑101 (MQ=255)
                                                                                      cgggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCg                 <  2:5916094/91‑1 (MQ=255)
                                                                                        ggcgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGc               >  1:3948528/9‑101 (MQ=255)
                                                                                          cgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGcgt             >  2:2902024/7‑101 (MQ=255)
                                                                                          cgtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGcgt             >  1:1927395/7‑101 (MQ=255)
                                                                                           gtgaactgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGcgtc            >  2:4752202/6‑101 (MQ=255)
                                                                                           gcgAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGcgtc            >  1:4269807/1‑101 (MQ=255)
                                                                                             gAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGNNCNNGGCCGGACCGTGACCACCGGcgtcgt          >  1:6725983/1‑101 (MQ=255)
                                                                                                ctgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGc       <  2:6289264/101‑1 (MQ=255)
                                                                                                ctgctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGc       >  2:915002/1‑101 (MQ=255)
                                                                                                  gctGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGcct     >  2:5272218/1‑99 (MQ=255)
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GGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCT.GAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGC  >  NC_014034/2155485‑2155685

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG