Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,176,472 G→C 100% A302G (GCG→GGG)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,176,4720G→C100.0% 1329.4 352A302G (GCG→GGG) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (179/173):  ref base (0/0):  total (179/173)

Only 100 of 358 total aligned reads displayed.

CCCAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176375‑2176569
                                                                                                  |                                                                                                 
 ctaGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGt                                                                                                <  2:6362317/99‑1 (MQ=255)
 ccAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGt                                                                                                <  1:6381772/101‑1 (MQ=255)
  cAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTc                                                                                               <  1:3757685/101‑1 (MQ=255)
     cATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGa                                                                                            >  1:5793165/1‑101 (MQ=255)
      aTTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGaa                                                                                           >  1:6585117/1‑101 (MQ=255)
      aTTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGaa                                                                                           >  1:4467072/1‑101 (MQ=255)
        tGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAAtt                                                                                         >  2:3092414/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTc                                                                                        >  1:3032056/1‑101 (MQ=255)
         gTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGCAATTc                                                                                        >  2:2311770/1‑101 (MQ=255)
          tttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCa                                                                                       <  1:1385678/101‑1 (MQ=255)
          tttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCa                                                                                       <  2:6113989/101‑1 (MQ=255)
          tttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCa                                                                                       <  1:2423633/101‑1 (MQ=255)
           ttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAc                                                                                      >  1:5645032/1‑101 (MQ=255)
              aGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCgg                                                                                   <  2:3252354/101‑1 (MQ=255)
               gcgcCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGc                                                                                  >  1:104181/1‑101 (MQ=255)
                cgcCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCt                                                                                 <  2:4618196/101‑1 (MQ=255)
                cgcCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCt                                                                                 <  2:2199430/101‑1 (MQ=255)
                  ccTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTc                                                                               <  1:1759169/101‑1 (MQ=255)
                   ctctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCg                                                                              >  2:3672990/1‑101 (MQ=255)
                   ctctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGAGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCg                                                                              >  2:24600/1‑101 (MQ=255)
                    tctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGt                                                                             >  1:6634145/1‑101 (MQ=255)
                     ctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTg                                                                            <  2:6633803/101‑1 (MQ=255)
                       tCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGcc                                                                          >  1:1635767/1‑101 (MQ=255)
                        ccGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCg                                                                         >  2:4094533/1‑101 (MQ=255)
                         cGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGt                                                                        <  1:4569075/101‑1 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       <  1:278053/101‑1 (MQ=255)
                          gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       >  2:5797201/1‑101 (MQ=255)
                           aGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTcc                                                                      <  2:5644742/101‑1 (MQ=255)
                            ggTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCt                                                                     <  1:3944392/101‑1 (MQ=255)
                              tGATCGGGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGTATTCACCGGCTTCGTGCCGTCCTTg                                                                   <  2:1485171/101‑1 (MQ=255)
                              tGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTg                                                                   >  1:5282109/1‑101 (MQ=255)
                               taacGAGAGGATGCCCCCGATCGAGCTGGCGGTGACCAGCGCCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTgg                                                                  <  2:736577/98‑1 (MQ=255)
                                aTCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGc                                                                 <  2:3741456/101‑1 (MQ=255)
                                aTCGAGGGGAGGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGc                                                                 <  2:6311128/101‑1 (MQ=255)
                                  cGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCAt                                                               <  2:4191706/101‑1 (MQ=255)
                                  cGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCAt                                                               <  1:4389036/101‑1 (MQ=255)
                                   gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              >  2:2998127/1‑101 (MQ=255)
                                   gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              <  2:2533473/101‑1 (MQ=255)
                                    aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             <  2:660532/101‑1 (MQ=255)
                                    aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             >  1:778584/1‑101 (MQ=255)
                                    aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             <  1:258582/101‑1 (MQ=255)
                                        gatgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggcgg                                                         <  2:4836790/96‑1 (MQ=255)
                                          tgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGgcggcg                                                       >  2:4410505/4‑101 (MQ=255)
                                            cccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGAt                                                     <  1:4820709/100‑1 (MQ=255)
                                            cccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGAt                                                     <  1:6874023/100‑1 (MQ=255)
                                             ctttcgatctgCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATc                                                    <  2:1760206/91‑1 (MQ=255)
                                              cccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCg                                                   <  2:4675311/101‑1 (MQ=255)
                                                cGATCGGTCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGcc                                                 <  2:122326/101‑1 (MQ=255)
                                                cGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGcc                                                 >  1:2187630/1‑101 (MQ=255)
                                                 gATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCt                                                >  1:350089/1‑101 (MQ=255)
                                                 gATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCt                                                >  1:860342/1‑101 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               <  2:6919677/101‑1 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               <  2:6011614/101‑1 (MQ=255)
                                                  aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGACCGCCTc                                               >  2:7058338/1‑101 (MQ=255)
                                                   tCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCg                                              <  2:346239/101‑1 (MQ=255)
                                                   tCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCg                                              >  1:6111224/1‑101 (MQ=255)
                                                     gggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGAt                                            <  1:6700380/101‑1 (MQ=255)
                                                     gggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGAt                                            <  2:1985976/101‑1 (MQ=255)
                                                      ggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATg                                           >  1:7055327/1‑101 (MQ=255)
                                                      ggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATg                                           >  1:3520935/1‑101 (MQ=255)
                                                       gCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGc                                          <  1:5924278/101‑1 (MQ=255)
                                                       gCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGc                                          >  2:3831878/1‑101 (MQ=255)
                                                         tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGccc                                        >  1:4789333/1‑101 (MQ=255)
                                                         tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGccc                                        <  1:3867039/101‑1 (MQ=255)
                                                           tCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCtt                                      <  1:1675802/101‑1 (MQ=255)
                                                            cGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTg                                     <  2:1920494/101‑1 (MQ=255)
                                                             ggTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTgg                                    <  1:3371516/101‑1 (MQ=255)
                                                              gTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGa                                   <  2:828201/101‑1 (MQ=255)
                                                                gACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAgg                                 <  1:4678452/101‑1 (MQ=255)
                                                                    aGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCAt                             <  1:3673139/101‑1 (MQ=255)
                                                                    aGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGAATAGACGGCGATCGCCTCGATGCCCTTGGAGGCCAt                             >  1:6332304/1‑101 (MQ=255)
                                                                     gCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATc                            >  2:286550/1‑101 (MQ=255)
                                                                      cGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCa                           >  2:919797/1‑101 (MQ=255)
                                                                      cGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCa                           <  1:697418/101‑1 (MQ=255)
                                                                        tcgccccGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGa                         <  1:1123745/98‑1 (MQ=255)
                                                                        tCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGa                         >  1:1270005/1‑101 (MQ=255)
                                                                         cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        <  1:3084662/101‑1 (MQ=255)
                                                                         cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        <  1:4094727/101‑1 (MQ=255)
                                                                         cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        >  2:5518012/1‑101 (MQ=255)
                                                                              cGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCggg                   >  2:511262/1‑101 (MQ=255)
                                                                               ggTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGa                  >  2:6719072/1‑101 (MQ=255)
                                                                                 tgtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAt                >  2:2103295/1‑101 (MQ=255)
                                                                                  gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtt               >  1:1578932/1‑101 (MQ=255)
                                                                                  gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtg               >  2:1724525/1‑100 (MQ=255)
                                                                                  gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAta               >  1:3233540/1‑100 (MQ=255)
                                                                                   ttGACCAAATCGAGCCCGTCGGAATTCCCCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTg              <  1:2274704/101‑1 (MQ=255)
                                                                                      aCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCAt           <  1:5547954/101‑1 (MQ=255)
                                                                                      aCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCAt           <  1:5451687/101‑1 (MQ=255)
                                                                                        gAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCg         >  1:2018425/1‑101 (MQ=255)
                                                                                         aaaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGa        >  1:4908998/1‑101 (MQ=255)
                                                                                         aaaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGa        >  1:432439/1‑101 (MQ=255)
                                                                                         aaaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGa        <  1:6805191/101‑1 (MQ=255)
                                                                                          aaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAg       >  2:1605404/1‑101 (MQ=255)
                                                                                          aaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAg       >  1:5132677/1‑101 (MQ=255)
                                                                                            tCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAggg     >  2:4492162/1‑100 (MQ=255)
                                                                                            tCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGt     <  1:1167527/101‑1 (MQ=255)
                                                                                             cGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTg    <  1:6448640/101‑1 (MQ=255)
                                                                                             cGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTg    <  1:286554/101‑1 (MQ=255)
                                                                                               aGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGgc  <  2:4789093/101‑1 (MQ=255)
                                                                                               aGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAATGCATCGAGGTGgc  >  1:1906897/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCCAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176375‑2176569

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG