Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,213 G→A 100% P55L (CCC→CTC)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2130G→A100.0% 1321.5 344P55L (CCC→CTC) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (172/172):  ref base (0/0):  total (172/172)

Only 100 of 354 total aligned reads displayed.

GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305
                                                                                                  |                                                                                                 
gCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGc                                                                                                 >  1:3987016/1‑101 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               >  2:6500277/1‑101 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               <  1:1652774/101‑1 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               <  1:735279/101‑1 (MQ=255)
   tGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAcc                                                                                              >  1:606569/1‑101 (MQ=255)
   tGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAcc                                                                                              >  2:2844304/1‑101 (MQ=255)
     aTCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAg                                                                                            >  1:4168644/1‑101 (MQ=255)
       cAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcc                                                                                          >  2:7020617/1‑101 (MQ=255)
       cAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcc                                                                                          <  2:4156546/101‑1 (MQ=255)
         aaaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcccc                                                                                        >  2:3863392/1‑101 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  1:5505141/101‑1 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  1:4370871/101‑1 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  1:3127823/101‑1 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  1:3526978/1‑101 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  2:5803824/1‑101 (MQ=255)
              cccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtctc                                                                                   >  1:6984012/1‑101 (MQ=255)
                cTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCgg                                                                                 >  2:4716191/1‑101 (MQ=255)
                 tttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGt                                                                                <  2:1335870/101‑1 (MQ=255)
                     ccccGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGcc                                                                            >  2:3141765/1‑101 (MQ=255)
                     ccccGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGcc                                                                            >  1:569039/1‑101 (MQ=255)
                      cccgcccgCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCt                                                                           <  1:3445440/101‑1 (MQ=255)
                      cccgcccgCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCt                                                                           >  1:6432586/1‑101 (MQ=255)
                       ccgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCtt                                                                          <  1:6587793/101‑1 (MQ=255)
                       ccgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCtt                                                                          <  2:5931320/101‑1 (MQ=255)
                        ctgccgcGCTGCGGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCttt                                                                         <  2:1719970/98‑1 (MQ=255)
                        cgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCttt                                                                         <  2:861439/101‑1 (MQ=255)
                         gcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTg                                                                        <  1:3819723/101‑1 (MQ=255)
                              cgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGaa                                                                   >  1:1731626/1‑101 (MQ=255)
                              cgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGaa                                                                   >  2:1582240/1‑101 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  2:4390941/1‑101 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  2:6435117/1‑101 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  2:3212916/1‑101 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  2:3677023/1‑101 (MQ=255)
                                gtGCAGCTGTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  2:4906451/100‑1 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 >  2:3761959/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  1:2653365/101‑1 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 >  2:546987/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 >  1:2454273/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 >  2:1311298/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  2:4556968/101‑1 (MQ=255)
                                 tGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGcc                                                                >  2:1047772/1‑100 (MQ=255)
                                   cAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcc                                                              <  1:2944586/101‑1 (MQ=255)
                                    aGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcct                                                             <  2:3036028/101‑1 (MQ=255)
                                     gCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcctt                                                            <  1:449758/101‑1 (MQ=255)
                                      cTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttc                                                           <  2:6042223/101‑1 (MQ=255)
                                      cTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttc                                                           >  1:1325076/1‑101 (MQ=255)
                                            aCCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCac                                                     >  2:151951/1‑100 (MQ=255)
                                            aCCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCAt                                                     >  2:628184/1‑101 (MQ=255)
                                               gccgcTATCTTCTGTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCtt                                                  <  2:1448275/101‑1 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  <  2:2478696/101‑1 (MQ=255)
                                               cccgcGATCTGCGTTTCCACATCGCCATCGNTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  <  1:990227/100‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  2:4801486/101‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  1:6887385/101‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  2:4545004/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              <  2:2556392/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              <  1:4992095/101‑1 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa                                             >  1:3620872/1‑101 (MQ=255)
                                                     aTCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaa                                            >  2:6690292/1‑101 (MQ=255)
                                                     aTCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaa                                            <  1:5918560/101‑1 (MQ=255)
                                                     aTCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTGCATCTTGACGaa                                            <  1:6302451/101‑1 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           >  1:4817344/1‑101 (MQ=255)
                                                        tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                         <  2:4299014/101‑2 (MQ=255)
                                                        tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                         >  1:6739202/1‑100 (MQ=255)
                                                            tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agggg                                   <  1:5382556/101‑3 (MQ=255)
                                                              tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGGCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggct                                 >  1:3888085/1‑97 (MQ=255)
                                                              tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                 >  1:2139463/1‑97 (MQ=255)
                                                              tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                 >  2:2095577/1‑97 (MQ=255)
                                                                cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                               <  2:3118987/101‑7 (MQ=255)
                                                                  caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                             <  1:2842278/101‑2 (MQ=255)
                                                                   aTCGCCATCTATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgt                            <  2:2224564/101‑3 (MQ=255)
                                                                   aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgt                            <  1:2522710/101‑3 (MQ=255)
                                                                   aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGCCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgt                            <  2:1271552/101‑3 (MQ=255)
                                                                    tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtc                           <  2:6365827/101‑4 (MQ=255)
                                                                    tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtc                           <  2:444281/101‑4 (MQ=255)
                                                                      gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcgg                         <  1:1308544/101‑6 (MQ=255)
                                                                      gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcgg                         <  1:1173905/101‑6 (MQ=255)
                                                                        cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtc                       <  2:800904/101‑8 (MQ=255)
                                                                         aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtct                      <  2:1546715/101‑9 (MQ=255)
                                                                          tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtctt                     <  2:6966139/101‑10 (MQ=255)
                                                                          tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtctt                     <  1:7020991/101‑10 (MQ=255)
                                                                           cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.g                     >  2:6189021/1‑101 (MQ=255)
                                                                           cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.g                     >  1:4060804/1‑101 (MQ=255)
                                                                           cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.g                     >  1:5740997/1‑101 (MQ=255)
                                                                            gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.gg                    >  1:102319/1‑100 (MQ=255)
                                                                            gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.gg                    <  2:2757341/101‑2 (MQ=255)
                                                                             aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   <  2:357225/101‑3 (MQ=255)
                                                                             aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   <  1:4632171/101‑3 (MQ=255)
                                                                                ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgat                <  2:5836909/101‑6 (MQ=255)
                                                                                 tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatc               >  1:1985593/1‑95 (MQ=255)
                                                                                  gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatca              >  2:728524/1‑94 (MQ=255)
                                                                                       cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacag          <  2:3363886/101‑1 (MQ=255)
                                                                                       cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacag          <  1:3148239/101‑1 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTTATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         >  2:1208804/1‑101 (MQ=255)
                                                                                         tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGc        >  2:5559158/1‑101 (MQ=255)
                                                                                          aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc       >  1:903236/1‑101 (MQ=255)
                                                                                           ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc      >  1:5572367/1‑101 (MQ=255)
                                                                                            gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcccc     <  2:5142684/101‑1 (MQ=255)
                                                                                             tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg    <  2:3925486/101‑1 (MQ=255)
                                                                                              cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCgg   >  2:6734748/1‑101 (MQ=255)
                                                                                               gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt  >  1:4788224/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 32 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG