Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,987	T→G	100%	intergenic (+260/‑245)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,987	0	T→G	100.0%	36.7	25	intergenic (+260/‑245)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (0/25):  ref base (0/0):  total (0/25)

AAACGCCGAACCG.CCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC..GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGG  >  NC_014034/2185918‑2186077                                                                              |                                                                                           aaaCGCCGAACCGCCCCC.TTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcc                                                              <  2:4176276/101‑1 (MQ=2)   ccGCCGACCCGCCCCC.TTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGC..C..CCCCGGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                            <  1:6478459/100‑1 (MQ=2)   aCGCCGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                            <  1:6207504/101‑1 (MQ=2)    cGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCa                                                           <  2:5735672/101‑1 (MQ=0)     gccgaccccccccc.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAg                                                          <  1:4208691/91‑1 (MQ=2)      ccGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGTTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                                                         <  2:2203682/101‑1 (MQ=0)      ccGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                                                         <  1:1013488/101‑1 (MQ=2)           ccG.CCCCTTTTCGCCTTGGCTCAAATATCCCGGGGGTCGGGGGGGGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                                    <  2:5196429/101‑1 (MQ=2)                 cc.TTTCCCCTTGGCTCAAATAGCCCGGGGGCCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgaccg                                              <  1:73419/101‑1 (MQ=9)                 cc.TTTCCCCTTGGCTCAAATACCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgaccg                                              <  2:4847608/101‑1 (MQ=9)                          cggggCTCAAATACCCGGGGGGCCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGa                                      <  1:5755541/98‑1 (MQ=2)                               cTCAAATATCCGGGGGGCCGGGGGGCGGG..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGcc                                 <  2:930069/101‑1 (MQ=9)                                ccAAATACCCGGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCt                                <  1:5313585/100‑1 (MQ=12)                                  aaaTACCCGGGGGGTCGGGGGGCGGG..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGt                              <  2:3569245/101‑1 (MQ=9)                                   atccgccggggggTCGGGGGGCGGACCC..CCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACCCCCCAGCGCCCCCGACCGACTTGCGACGGCCTGTc                             <  1:5413274/93‑1 (MQ=2)                                   aaTATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGGTGCCTCC.GGCG.AAGCCCCAGGCGGAACAACCCACCGCCCCCGACCGCCCTGCGACgggccggc                             >  2:6222610/1‑95 (MQ=2)                                         cggggggGCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaa                       <  1:5564043/99‑1 (MQ=9)                                            ggggTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg                    <  1:1095722/101‑1 (MQ=18)                                            ggggCCGGGGGGGGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg                    <  2:4854786/101‑1 (MQ=12)                                               gTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGccc                 <  2:6535218/101‑1 (MQ=18)                                                    ggggCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCca            <  1:6249211/101‑1 (MQ=18)                                                        ggcggagccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcagg          <  2:4187299/92‑1 (MQ=18)                                                           ggagccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcag       <  2:6946075/95‑1 (MQ=21)                                                            ggcccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcagg      <  1:1308938/96‑1 (MQ=21)                                                              cccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGAc    <  1:6450873/98‑1 (MQ=25)                                                                cccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACgg  <  1:2305232/100‑1 (MQ=25)                                                                              |                                                                                           AAACGCCGAACCG.CCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC..GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGG  >  NC_014034/2185918‑2186077 Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 20 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG