Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,186,012	G→A	100%	intergenic (+285/‑220)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,186,012	0	G→A	100.0%	196.8	66	intergenic (+285/‑220)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (32/34):  ref base (0/0):  total (32/34)

AAACGCCGAACCG.CCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC..GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185918‑2186109                                                                                                       |                                                                                                  aaaCGCCGAACCGCCCCC.TTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcc                                                                                              <  2:4176276/101‑1 (MQ=2)   ccGCCGACCCGCCCCC.TTTCCCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGC..C..CCCCGGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                                                            <  1:6478459/100‑1 (MQ=2)   aCGCCGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                                                            <  1:6207504/101‑1 (MQ=2)    cGCCGAACCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCa                                                                                           <  2:5735672/101‑1 (MQ=0)     gccgaccccccccc.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAg                                                                                          <  1:4208691/91‑1 (MQ=2)      ccGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGTTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                                                                                         <  2:2203682/101‑1 (MQ=0)      ccGACCCGCCCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                                                                                         <  1:1013488/101‑1 (MQ=2)           ccG.CCCCTTTTCGCCTTGGCTCAAATATCCCGGGGGTCGGGGGGGGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                                                                    <  2:5196429/101‑1 (MQ=2)                 cc.TTTCCCCTTGGCTCAAATAGCCCGGGGGCCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgaccg                                                                              <  1:73419/101‑1 (MQ=9)                 cc.TTTCCCCTTGGCTCAAATACCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgaccg                                                                              <  2:4847608/101‑1 (MQ=9)                          cggggCTCAAATACCCGGGGGGCCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGa                                                                      <  1:5755541/98‑1 (MQ=2)                               cTCAAATATCCGGGGGGCCGGGGGGCGGG..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGcc                                                                 <  2:930069/101‑1 (MQ=9)                                ccAAATACCCGGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCt                                                                <  1:5313585/100‑1 (MQ=12)                                  aaaTACCCGGGGGGTCGGGGGGCGGG..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGt                                                              <  2:3569245/101‑1 (MQ=9)                                   atccgccggggggTCGGGGGGCGGACCC..CCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACCCCCCAGCGCCCCCGACCGACTTGCGACGGCCTGTc                                                             <  1:5413274/93‑1 (MQ=2)                                   aaTATCCCGGGGGTCCGGGGGCGGA..GCCCCC..GGCGGGGTGCCTCC.GGCG.AAGCCCCAGGCGGAACAACCCACCGCCCCCGACCGCCCTGCGACgggccggc                                                             >  2:6222610/1‑95 (MQ=2)                                         cggggggGCGGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaa                                                       <  1:5564043/99‑1 (MQ=9)                                            ggggTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg                                                    <  1:1095722/101‑1 (MQ=18)                                            ggggCCGGGGGGGGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAg                                                    <  2:4854786/101‑1 (MQ=12)                                               gTCCGGGGGCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGccc                                                 <  2:6535218/101‑1 (MQ=18)                                                    ggggCGGA..GCCCCC..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCca                                            <  1:6249211/101‑1 (MQ=18)                                                        ggcggagccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcagg                                          <  2:4187299/92‑1 (MQ=18)                                                           ggagccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcag                                       <  2:6946075/95‑1 (MQ=21)                                                            ggcccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcagg                                      <  1:1308938/96‑1 (MQ=21)                                                              cccccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGAc                                    <  1:6450873/98‑1 (MQ=25)                                                                cccc..GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACgg                                  <  1:2305232/100‑1 (MQ=25)                                                                                 cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                     >  2:4749026/10‑101 (MQ=255)                                                                                   tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt                   >  1:559245/8‑101 (MQ=255)                                                                                   tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt                   <  1:2348851/94‑1 (MQ=255)                                                                                    cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  1:2330687/7‑101 (MQ=255)                                                                                    cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  2:1428739/7‑101 (MQ=255)                                                                                    cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  2:1393933/7‑101 (MQ=255)                                                                                    cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  2:7073311/7‑101 (MQ=255)                                                                                    cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                  >  1:5769694/7‑101 (MQ=255)                                                                                     gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 >  1:4985067/6‑101 (MQ=255)                                                                                     gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc                 >  1:2008492/6‑101 (MQ=255)                                                                                      ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                <  1:6748721/97‑1 (MQ=255)                                                                                      ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                <  1:5491613/97‑1 (MQ=255)                                                                                      ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  2:1615560/5‑101 (MQ=255)                                                                                      ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg                >  2:2320986/5‑101 (MQ=255)                                                                                       gcaaaGCCCGAGGCGAAGCACCCCAGCGCCACCGACCGACGTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg               <  1:4343738/98‑1 (MQ=255)                                                                                       gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg               <  2:7119248/98‑1 (MQ=255)                                                                                       gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg               <  2:1634009/98‑1 (MQ=255)                                                                                         aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc             <  2:2550951/100‑1 (MQ=255)                                                                                          aaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAcc            >  2:3977163/1‑101 (MQ=255)                                                                                            gcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCgg          >  2:470032/3‑101 (MQ=255)                                                                                            gcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCgg          >  2:1964673/3‑101 (MQ=255)                                                                                             cccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCggg         >  1:5890139/2‑101 (MQ=255)                                                                                             cccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCggg         <  2:1263318/100‑1 (MQ=255)                                                                                              ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        >  1:625895/1‑101 (MQ=255)                                                                                              ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt        >  1:216691/1‑101 (MQ=255)                                                                                               cGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGtt       >  2:5542068/1‑101 (MQ=255)                                                                                                gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTg      >  2:600665/1‑101 (MQ=255)                                                                                                gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTg      >  1:5816968/1‑101 (MQ=255)                                                                                                gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTg      >  1:4839344/1‑101 (MQ=255)                                                                                                gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTg      >  2:7102675/1‑101 (MQ=255)                                                                                                gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGCTg      >  2:1758611/1‑101 (MQ=255)                                                                                                gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAAGCCCTGCCGCGCAAAAGCCCGGCCAGGCAGGACGGCCATGCCTTGACCCTTCGGGCCGGGTTg      >  2:3166828/1‑101 (MQ=255)                                                                                                 aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  1:933131/1‑101 (MQ=255)                                                                                                 aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     >  1:6096905/1‑101 (MQ=255)                                                                                                 aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg     <  1:3977338/101‑1 (MQ=255)                                                                                                  ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg    >  2:4933081/1‑101 (MQ=255)                                                                                                   gCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGa   >  1:6894217/1‑101 (MQ=255)                                                                                                   gCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGa   <  1:387692/101‑1 (MQ=255)                                                                                                   gCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGa   >  2:2069394/1‑101 (MQ=255)                                                                                                    cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGaa  >  1:306456/1‑101 (MQ=255)                                                                                                    cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGaa  >  2:5137115/1‑101 (MQ=255)                                                                                                    cGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGGCGCGCAAAAGCCCGGCCAGGCAGGACGGCGATGCCCTGACCCTTCGGACCGGGTTcggcc  >  2:3940192/1‑99 (MQ=255)                                                                                                       |                                                                                                  AAACGCCGAACCG.CCCC.TTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC..GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGGAA  >  NC_014034/2185918‑2186109 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG