Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,015 G→C 7.2% T214T (ACC→ACG RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0150G→C 7.2% 0.0 ‑0.8 0.02544T214T (ACC→ACGRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (88/102):  ref base (1157/1190):  total (1247/1297)
Fisher's exact test strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 3064 total aligned reads displayed.

CGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGG  >  NC_014034/221915‑222115
                                                                                                    |                                                                                                    
ccgtgggatcggtcgtgtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcg                                                                                                      >  2:5418678‑M1/42‑101 (MQ=255)
 gcgcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGAGCCGTGGGAtcgg                                                                                                     <  1:6310798/101‑1 (MQ=255)
 cgtgggatcggtcggatccgtgggatcggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcgg                                                                                                     <  1:1696301‑M1/61‑1 (MQ=255)
        tcggtcgggtccgtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                                                                                              >  2:1921974‑M1/34‑101 (MQ=255)
         cggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                                                             >  2:1798244‑M1/33‑101 (MQ=255)
          ggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                                                                                            <  1:3775589‑M1/70‑1 (MQ=255)
              gTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTggg                                                                                        >  2:6655895/1‑101 (MQ=255)
                atccgtgggatcggtcggatccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGgag                                                                                      <  1:2872920‑M1/76‑2 (MQ=255)
                 tccgtgggatcggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                                     >  2:554523‑M1/25‑101 (MQ=255)
                  gcgtggggtccgtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                                                    >  1:4182896‑M1/24‑101 (MQ=255)
                  ccgtgggatcggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                                                    <  1:3878000‑M1/78‑1 (MQ=255)
                   cgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcgg                                                                                   <  2:2040364‑M1/79‑1 (MQ=255)
                    gtgggatcggtcgggtccgtcGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                                                                                  >  2:1085550‑M1/22‑101 (MQ=255)
                    ggcGGATGCGGGGGCTCGGTCGGATCGGGCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                                                                                  <  1:1915966/99‑1 (MQ=255)
                     tgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                                 >  1:5919055‑M1/21‑101 (MQ=255)
                             gtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGgtcccg                                                                         <  2:5008185‑M1/89‑3 (MQ=255)
                             gtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGt                                                                         >  2:2152824‑M1/13‑101 (MQ=255)
                               cggatccgtgggatcggtcgggtccgtgggatcggtcgggtccgtgGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTgg                                                                       >  1:406842/11‑101 (MQ=11)
                                  gtccgtcggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAt                                                                    <  2:4010250/97‑1 (MQ=11)
                                   tcggtcggGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtc                                                                   <  1:3397289/101‑1 (MQ=11)
                                   tccttgcgatcggtcgggtccgtgggatcggtcgggtccgtggGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtc                                                                   <  2:3661550/94‑1 (MQ=14)
                                   tcctgcggATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                                                   <  1:6932484/96‑1 (MQ=11)
                                    ccctgcggtcggtcgggtccgtgggatcggtcgggtccgtgggaTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcg                                                                  <  2:6901368/93‑1 (MQ=14)
                                     cgtgggatcggacggatccgtgggatcggtcgggtccgtgGGATCGGCCGGATCCGTGGGATCGGTCGGACCCGTGGATTCGCTCGGACACGTGGGCtcgt                                                                 >  2:318551/5‑100 (MQ=0)
                                     cgtcggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGGCGGGGCCGTGGGCtcgg                                                                 >  1:3000718/2‑101 (MQ=11)
                                       tgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                                               >  1:6163126‑M1/3‑101 (MQ=255)
                                        cggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtcg                                                              >  1:1793955/1‑101 (MQ=14)
                                         ggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTGGGATCCGTGGGATCGGTCGGGCCGGTGGGATCGGTCGGATCGGTGGGGtcggtggg                                                             >  2:1508780/1‑101 (MQ=14)
                                          gatcggtcggatccgtgggatcggtcgggtccgtggGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtcgga                                                            >  2:4880912/1‑100 (MQ=11)
                                           atcggtcgggttcgtaggaccgggcgggtccgtgggAGCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGtccgtgggat                                                           <  2:515142/101‑3 (MQ=11)
                                           atcggtcgggtccgtgggatcggtcgggtcggtgggGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGt                                                           <  1:1350961/101‑1 (MQ=11)
                                           atcggtcgggtccgtgggatcggtcgggtccgtgggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGtccgtgggct                                                           >  2:6362470/1‑99 (MQ=255)
                                            gcggtcggggccgtgggatcggtcgggtccgtaggatCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTc                                                          <  2:6219034/100‑1 (MQ=14)
                                             cggtcgggtccgtgggatcggtcgggtccgtcggatCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTAggggag                                                         >  2:4273165/1‑98 (MQ=11)
                                             cggtcggatccgtgggatcggtcggatccgtaggatCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                                                         >  1:3940943/1‑101 (MQ=11)
                                              ggtggggtccgttggatcgggcgggtccgtggggtcCGTCTGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                                                        <  1:1104417/101‑1 (MQ=14)
                                              ggtcgggtccgtcggatcggtcgggtccgtgggatcGGTCGGGACCGTGGGATCGGACGGGTCGGGGGGAGCGGTCGGGTCTGGCGGAtcggttggaacag                                                        >  2:2713127/1‑96 (MQ=2)
                                              cgtcggatccgtgggatcggtcggatccgtgggatcgGTCGGATCCGTGGGATCGGTCGGGTCGGTGGGATCGGTCGGGTCGGTGGGCTCGGTCGGGGCCg                                                        >  2:751003/2‑101 (MQ=11)
                                               gtcgggtccgtgggatctgtcgggtccgtggggtccGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                                                       <  2:2823887/101‑1 (MQ=14)
                                               gtcggatccgtgggatcggtcggatccgtaggatcgGTCGGGTCCGTAGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGt                                                       >  2:5286626/1‑101 (MQ=11)
                                               gacaggtccgtgtgatcggtcggttccgtgggatcggtcgGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGt                                                       <  2:30885/97‑1 (MQ=11)
                                                tcggatccgtgggatcggccggatccgtaggatcggTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGtc                                                      <  1:350792/101‑2 (MQ=11)
                                                gcggatccgtgggatcggtcgggtccgggggatcggtcGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTg                                                      >  2:1653318‑M2/2‑100 (MQ=255)
                                                 cgggtccgtgggatcggtcgggtccgtgggatcggtCGGGTCCGTAGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGtgg                                                     >  2:6073868/1‑99 (MQ=11)
                                                 cgggtccgtcggatcggtcgggtccgtgggatcggtCGGGTCCGTCGGATCGGTGGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGGGCCGtcg                                                     >  2:345610/1‑99 (MQ=11)
                                                  ggatccgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGgg                                                    >  1:4709201/4‑101 (MQ=11)
                                                   ggtccgtgggatcggtcgggtccgtggggtccgtcgGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGACCGTGGGATCGGTCGGGTCCgcggga                                                   >  2:5936336/1‑96 (MQ=11)
                                                    gtccttgcgatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAt                                                  <  2:3799172/101‑1 (MQ=11)
                                                    ggcggtgggatcggtcggggccgtgggatcggtcggatccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTgggat                                                  >  1:81807‑M2/5‑96 (MQ=255)
                                                    atccgtggggtccgtcgggtccgtgggatcggtcggGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGGTCCGTGgggt                                                  >  2:6768645/2‑99 (MQ=11)
                                                     tccgtgggctcggtcggctccgtgggatccgtcggATCCGTGGGATCCGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                                 <  2:1369208/101‑1 (MQ=14)
                                                      ccgtgggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcg                                                >  2:2342930‑M2/1‑94 (MQ=255)
                                                      ccgtgggatcggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGATGGGTCGGGTCCGTGGGGtcg                                                >  2:4364882/1‑101 (MQ=11)
                                                      ccgtgggatcggtcggatccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTggggtcg                                                <  1:2183742‑M2/101‑8 (MQ=255)
                                                       cgtgggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGgggcggg                                               >  1:1000254/1‑96 (MQ=11)
                                                       cgtgggatcggtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCCGGGGGGCCCGTCGGATCCGGGGGAtggg                                               >  2:5674344/1‑101 (MQ=11)
                                                        gttggatcggtcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGAtcggt                                              <  1:257199/98‑1 (MQ=11)
                                                         tgggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtc                                             <  1:2328361‑M2/101‑11 (MQ=255)
                                                         tgggatcggtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGGGGGATCGGTCGGGCCCGTGGGAtaggtc                                             >  2:2942974/1‑101 (MQ=11)
                                                            gatcggtcggatccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgga                                          <  1:485679‑M2/101‑14 (MQ=255)
                                                             gtgtgtggggtccgtggggacggcgggGTCCGTGGGATCGGTCGGGTCCGTGGGATGGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggt                                         <  1:2776283/94‑1 (MQ=11)
                                                              gcgggcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtc                                        <  1:3606092/100‑1 (MQ=11)
                                                                ggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcggatccg                                      >  1:5643725/1‑101 (MQ=11)
                                                                  tcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtg                                    >  1:5521348‑M2/1‑82 (MQ=255)
                                                                    gggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtggg                                  >  1:5126912‑M2/1‑80 (MQ=255)
                                                                    ggatccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtggg                                  <  1:3264487‑M2/101‑22 (MQ=255)
                                                                    ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATGCGTGGGATCGGACGGGTTCGTGGGATCGgtcgggtccgtggg                                  >  2:892953/4‑101 (MQ=14)
                                                                     ggtccgtgggatcggtcggGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtggga                                 <  1:430245‑M2/101‑23 (MQ=255)
                                                                     gatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgga                                 <  1:6671309/99‑2 (MQ=255)
                                                                      gtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtggggg                                <  2:70690‑M2/101‑24 (MQ=255)
                                                                       tccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcggatccgtgggatc                               >  1:6027100‑M2/1‑77 (MQ=255)
                                                                        ccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggatcg                              <  2:1235509/101‑2 (MQ=255)
                                                                         cgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGTATCCGTGGGATCGGTCGGGTCCGGgggatcggtcgggtccgtgggatcgg                             <  2:3568006‑M2/101‑27 (MQ=255)
                                                                           tgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtc                           <  1:708711‑M2/101‑29 (MQ=255)
                                                                           ggggatctgtcggGGCCGTGGCTTCCGCCGGGTCCGTGGGACCGGCCGGACCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTcggatccgtgggatcggtc                           <  2:4157346/100‑2 (MQ=2)
                                                                            gggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTgg                          <  2:4477681/101‑1 (MQ=14)
                                                                            cggatcggtgggaTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtcg                          <  1:5785754‑M2/100‑30 (MQ=255)
                                                                             gggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGCCGGGTCCGTCGGGTCCGTggg                         >  1:538289/10‑101 (MQ=14)
                                                                             ggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtcgg                         <  2:2618362‑M2/101‑31 (MQ=255)
                                                                               gtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAt                       <  1:4436715/97‑1 (MQ=11)
                                                                                tcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtc                      <  1:527405/101‑1 (MQ=14)
                                                                                tcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGtc                      <  1:953903/101‑1 (MQ=11)
                                                                                 cggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtcc                     <  2:4595832/101‑2 (MQ=11)
                                                                                 ccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcg                     <  2:3902590/96‑1 (MQ=14)
                                                                                  ggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtcgggtccg                    <  2:580773‑M2/101‑36 (MQ=255)
                                                                                   gtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggt                   >  1:2451467/1‑101 (MQ=11)
                                                                                     cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcg                 <  1:1234295/101‑1 (MQ=14)
                                                                                       ggtccGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTgggatcggtcgggtccgtgggatcggtcgggtccgtggga               >  1:6564606‑M2/3‑61 (MQ=255)
                                                                                       gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGGGGGATCGGGCGGGGCCGGGGGGTGCGTCGGacacgggggg               >  2:3310248/1‑92 (MQ=11)
                                                                                        gtcCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtcgggtccgtgggct              <  1:1526010‑M2/100‑42 (MQ=255)
                                                                                           cgtgtgcgcggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTcca           <  2:4715351/93‑2 (MQ=14)
                                                                                            gTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcggt          <  1:3128977‑M2/101‑46 (MQ=255)
                                                                                            gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTgggatcggtcggatccgtgggatcggtcggatccgtgggatcggt          <  1:45705‑M2/101‑46 (MQ=255)
                                                                                             tGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtc         <  1:2675690‑M2/101‑47 (MQ=255)
                                                                                             tGGGAGCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcggatccgtgggatcggtcgggtccgtgggatcggtc         >  1:4978652‑M2/1‑55 (MQ=255)
                                                                                              gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtcgggtccgtggggtccgtcg        <  1:6801789‑M2/101‑48 (MQ=255)
                                                                                              aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGtcg        >  2:2487386/2‑99 (MQ=14)
                                                                                                gATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcggatccgtgggatcggtcggatccgtgggatcggtcgga      <  2:6529770‑M2/101‑50 (MQ=255)
                                                                                                  tcggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtc    <  1:2039344/101‑1 (MQ=14)
                                                                                                   cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGGGCGGTCGGGTCCGTCGGATCCGTGGGGGCCGCCGGGGCCGGGGGCTCGGAGGGGCCCGTCGGAtcg   >  1:6439326/1‑101 (MQ=9)
                                                                                                    |                                                                                                    
CGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGG  >  NC_014034/221915‑222115

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG