Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,081 C→G 22.2% P192P (CCG→CCC RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0810C→G22.2% 0.0 ‑0.5 ‑0.0695P192P (CCG→CCCRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (75/80):  ref base (276/264):  total (351/344)
Fisher's exact test strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

Only 100 of 987 total aligned reads displayed.

GATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGG  >  NC_014034/221975‑222181
                                                                                                          |                                                                                                    
      ttggatccgtcgggtccgtcggATCGGTCGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtc                                                                                                      <  1:3056488/96‑1 (MQ=11)
      tcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtc                                                                                                      <  1:3483223/101‑1 (MQ=255)
      gtggatccgtcggctccgtcggATCGTTTGGTTCCGTCGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtc                                                                                                      <  1:1338128/96‑1 (MQ=11)
        ggttccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggg                                                                                                    <  2:4000300/98‑1 (MQ=11)
        gggtccgtggggtccggcggATGCGTGGGATCCGGCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggg                                                                                                    <  1:3404679/101‑1 (MQ=11)
        ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATGCGTGGGATCGGACGGGTTCGTGGGATCGgtcgggtccgtggg                                                                                                    >  2:892953/4‑101 (MQ=14)
        ggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGgtcgggtccgtcgg                                                                                                    >  1:5320071/4‑101 (MQ=11)
          atccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGGCGGGTCCGTCGGATCCggcgggtccgtcggag                                                                                                  >  2:4375266/2‑99 (MQ=11)
          agcggtgggatcggtcggGTCGGTGGGATGGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGTTGGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                                  <  2:4540649/97‑3 (MQ=11)
           gccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgggtc                                                                                                 <  2:6135123/100‑1 (MQ=11)
            ccgtgggatcggtcggATCCGTGGGATGCGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggatcg                                                                                                <  1:4964801/101‑2 (MQ=14)
            ccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggatcg                                                                                                <  1:1714429/101‑2 (MQ=255)
            ccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGggcgggtccggggggtcg                                                                                                >  1:737239/1‑100 (MQ=11)
              gtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggt                                                                                              >  1:6775495/1‑101 (MQ=11)
              gtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTcgggtccgtcggatccgt                                                                                              >  1:6171202/1‑101 (MQ=11)
              ggaggatcggtcggGTCCGTGGGATCGGTCGGGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgt                                                                                              <  1:3586244/98‑1 (MQ=11)
               ggcggtcggtcggATCGGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                                                                                             <  2:477260/96‑2 (MQ=11)
                gatctccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtca                                                                                            <  1:2236071/94‑3 (MQ=11)
                 gggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                                           <  1:1553607/92‑1 (MQ=14)
                 gggtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGGTCCGTGGGATCGGTcgggtccgtcggatccgtctg                                                                                           >  2:323856/7‑98 (MQ=11)
                 ggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTcgg                                                                                           >  1:5512727/1‑101 (MQ=11)
                  ggtcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgggg                                                                                          <  2:2851123/99‑2 (MQ=11)
                  ggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGa                                                                                          >  1:5176349/9‑101 (MQ=11)
                  ggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGGCCGTGGGa                                                                                          >  1:694055/9‑101 (MQ=11)
                  gatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGa                                                                                          <  2:111482/101‑1 (MQ=255)
                   atcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAt                                                                                         <  1:2930264/101‑1 (MQ=11)
                    tccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtc                                                                                        <  2:7051388/98‑1 (MQ=11)
                    cccgtgggATCGGTCGGATCCGTTGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtc                                                                                        <  2:1456465/95‑1 (MQ=14)
                     tggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcg                                                                                       <  1:1873006/100‑1 (MQ=14)
                     cggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCGGTCGGGTCGGTgggggcg                                                                                       >  2:2929820/1‑97 (MQ=14)
                      ggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccg                                                                                      >  1:1991692/1‑99 (MQ=11)
                       gtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggt                                                                                     >  1:7071159/4‑101 (MQ=14)
                        tgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                    >  1:2550335/3‑101 (MQ=14)
                        tcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGGtcgggc                                                                                    >  2:1991954/1‑99 (MQ=14)
                        tcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgtg                                                                                    >  1:6667948/1‑100 (MQ=11)
                         cggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTGGGGTCCGTGGGGTCGGTCGGATCGGTGGGGGCAGTGCGAtcggtcg                                                                                   >  2:3005723/1‑101 (MQ=11)
                         cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                                   <  1:1234295/101‑1 (MQ=14)
                          gggtCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcgg                                                                                  <  1:6542474/98‑1 (MQ=14)
                          ggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCCGTCGGGtccgtcgg                                                                                  >  2:6570808/1‑101 (MQ=11)
                           gATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGa                                                                                 >  2:6699828/1‑101 (MQ=17)
                           gATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGGGGGATCGGGCGGGGCCGGGGGGTGCGTCGGacacgggggg                                                                                 >  2:3310248/1‑92 (MQ=11)
                             tgggtcggATCGGTGGGGTACGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATc                                                                               <  1:4636627/95‑1 (MQ=17)
                             tcggtcgtaTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATc                                                                               <  2:6304531/93‑1 (MQ=17)
                              gggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATcc                                                                              <  1:331522/96‑1 (MQ=17)
                              ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTcc                                                                              <  1:1096752/101‑1 (MQ=14)
                              ccGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTcc                                                                              <  1:2868168/101‑1 (MQ=14)
                                gtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCgc                                                                            >  2:2408038/4‑100 (MQ=17)
                                  aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTCGGATCGGTCGGGTCCGtcg                                                                          >  2:4161438/2‑99 (MQ=14)
                                   ggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                                                                         >  2:2720546/1‑101 (MQ=255)
                                    ggtcggtcggGTCCGTGGGATCGGTCGGAGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGa                                                                        <  1:2063368/99‑1 (MQ=17)
                                     gtcggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAt                                                                       <  1:5876085/100‑1 (MQ=17)
                                     atgcgtcggGTCGGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAt                                                                       <  2:2145274/97‑1 (MQ=17)
                                      tcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTGGGGTCGGTGGGGtc                                                                      >  2:4958729/1‑101 (MQ=11)
                                      tcgggcggGTCGGTCGGCTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtc                                                                      <  2:1849273/101‑1 (MQ=14)
                                         gtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggt                                                                   >  1:50470/4‑101 (MQ=255)
                                          tggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                                                  <  2:2559525/99‑1 (MQ=255)
                                           cggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg                                                                 >  1:4327610/1‑101 (MQ=17)
                                            gggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGCTCGGTTGGATCCGTGGGAtcggtcgg                                                                >  1:4848464/1‑101 (MQ=255)
                                              ggccGTGGGCTCGGTCGGGTCCGTGGGCTCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                                                              <  2:3128811/99‑1 (MQ=255)
                                              gTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCgggg                                                              >  2:5778364/1‑100 (MQ=255)
                                              gTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCcggc                                                              >  2:6042517/1‑100 (MQ=255)
                                                ccGTCGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTcc                                                            <  2:3624617/101‑1 (MQ=17)
                                                 ggTGGGGTCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCg                                                           <  1:5728282/100‑1 (MQ=14)
                                                  gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGCGTCCGTCGGGTCCGTGGGGCCGGTGGGATCGGCGGGGCCGGGCGGGTCCgc                                                          >  1:5630883/1‑100 (MQ=17)
                                                   tGGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCGGTGGGGTCGGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTg                                                         <  1:3788759/101‑1 (MQ=14)
                                                   tGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTg                                                         >  1:400512/1‑101 (MQ=17)
                                                    gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                        >  1:6391492/1‑101 (MQ=255)
                                                    gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                        <  1:924995/101‑1 (MQ=255)
                                                    cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgg                                                        >  1:2464323/2‑101 (MQ=18)
                                                    aggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATGCGGCGGGTCGGtcg                                                        >  2:1004871/2‑99 (MQ=14)
                                                     ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGACCGGTCGGGCCCGGGGGATCGGTCGGGCCCGTagg                                                       >  1:1779743/1‑101 (MQ=17)
                                                      gATCGGGCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTagga                                                      <  1:4505368/101‑2 (MQ=17)
                                                       gtccggcggATCCGGGGGACCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggat                                                     <  2:1551144/95‑3 (MQ=14)
                                                       aTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGGGGGGc                                                     >  1:1022575/1‑101 (MQ=255)
                                                       aTCGGTCGGGTCCGTGGGATCGGTCGGGTCC.TAGGATCGGTCGGGTCCGTCGGGTCCCTGGGATCGGTCGGATCCGGGGGATCGGCCGGGTCCGTggggtg                                                    >  2:1249489/1‑99 (MQ=18)
                                                       aTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTaggat                                                     >  2:6927156/1‑99 (MQ=17)
                                                        tcggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatc                                                    <  1:412259/101‑4 (MQ=14)
                                                         cggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcg                                                   <  2:5072335/101‑5 (MQ=14)
                                                         cggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcg                                                   <  2:3776674/101‑5 (MQ=14)
                                                          ggtcggATCCGTTGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTTGGGTCCGTCGGATCGGTCGGGTCCGTgggatcgg                                                  <  2:4649635/101‑6 (MQ=14)
                                                          ggtcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGGgggatcgg                                                  >  2:5599755/1‑96 (MQ=17)
                                                           gtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggg                                                 >  2:3693105/1‑95 (MQ=14)
                                                            tcggactggggggATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtg                                                <  1:6063849/91‑1 (MQ=18)
                                                             cggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcg                                               >  1:2669488/1‑93 (MQ=11)
                                                               gATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggccggg                                             >  2:745452/1‑91 (MQ=14)
                                                                          tcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAcc                                  <  2:5456834/101‑1 (MQ=255)
                                                                          tcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGGCGGAcc                                  >  1:2379462/1‑101 (MQ=255)
                                                                                gggTCCGTGGGATCGGTCCGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                            <  2:3910001/101‑1 (MQ=255)
                                                                                ggctCCGTAGGATGGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                            <  2:265503/98‑1 (MQ=255)
                                                                                 gggccGTAGGGTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGTACCGCTGTcg                           <  2:5328532/98‑1 (MQ=255)
                                                                                     cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgc                       >  2:2215020/1‑101 (MQ=255)
                                                                                       gaGGATTGGTCGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgccg                     <  1:388856/100‑1 (MQ=255)
                                                                                        aGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgccgc                    >  2:3480072/1‑101 (MQ=255)
                                                                                           aTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCgg                 >  2:5192416/1‑101 (MQ=255)
                                                                                             cggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGAc               >  2:5920839/1‑101 (MQ=255)
                                                                                             cggtcggGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGAc               >  2:6820171/1‑101 (MQ=255)
                                                                                                  gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAcc          >  2:835303/1‑101 (MQ=255)
                                                                                                   gggccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAccc         <  2:1718547/98‑1 (MQ=255)
                                                                                                      cggtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAgg      <  1:317670/99‑1 (MQ=255)
                                                                                                        gtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggcc    >  2:6468536/1‑101 (MQ=255)
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GATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGG  >  NC_014034/221975‑222181

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG