Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,232,209 T→C 100% T767A (ACC→GCC)  RCAP_rcc02068 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,232,2090T→C100.0% 1176.0 315T767A (ACC→GCC) RCAP_rcc02068ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (141/174):  ref base (0/0):  total (141/174)

Only 100 of 322 total aligned reads displayed.

ACCGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGTGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCGCCGCC  >  NC_014034/2232111‑2232306
                                                                                                  |                                                                                                 
 ccGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGAt                                                                                                >  1:2910717/1‑101 (MQ=255)
 ccGATCTGGGCCGGCGACAGCGTCGCCGCCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGAt                                                                                                <  2:6866327/101‑1 (MQ=255)
  cGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATc                                                                                               >  1:2384816/1‑101 (MQ=255)
  cGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATc                                                                                               >  1:2233505/1‑101 (MQ=255)
  cGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATc                                                                                               <  1:6272045/101‑1 (MQ=255)
   gATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                              <  1:4961493/101‑1 (MQ=255)
   gATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                              <  1:996580/101‑1 (MQ=255)
   gATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                              >  2:5097887/1‑101 (MQ=255)
   gATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCt                                                                                              >  2:5756542/1‑101 (MQ=255)
    gtCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTg                                                                                             <  1:3647550/100‑1 (MQ=255)
     tCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTgg                                                                                            <  2:2102812/101‑1 (MQ=255)
      cTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTggg                                                                                           >  2:4480606/1‑101 (MQ=255)
         ggCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCg                                                                                        <  2:2026829/101‑1 (MQ=255)
           ccGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAg                                                                                      <  1:1095276/101‑1 (MQ=255)
           ccGCCGACACCGTCGCACGCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAg                                                                                      <  2:3196272/101‑1 (MQ=37)
             gTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCt                                                                                    >  2:1214475/1‑101 (MQ=255)
             gTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCt                                                                                    >  2:1640207/1‑101 (MQ=255)
                 gcAGCGGCGCCCCCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGc                                                                                <  2:5303129/100‑1 (MQ=255)
                 aCAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGc                                                                                >  1:6775967/1‑101 (MQ=255)
                 aCAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGc                                                                                >  2:5945316/1‑101 (MQ=255)
                  cAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGcc                                                                               >  1:6963799/1‑101 (MQ=255)
                   aGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGccc                                                                              <  2:2567244/101‑1 (MQ=255)
                   aGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCACccc                                                                              <  1:6850007/101‑1 (MQ=255)
                    gCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGcccc                                                                             <  2:4636516/101‑1 (MQ=255)
                    gCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGcccc                                                                             <  1:4035774/101‑1 (MQ=255)
                     cGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCt                                                                            <  2:3480498/101‑1 (MQ=255)
                     cGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCt                                                                            >  1:885064/1‑101 (MQ=255)
                     cGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCt                                                                            <  1:595032/101‑1 (MQ=255)
                      gTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCtt                                                                           >  2:4786833/1‑101 (MQ=255)
                       tCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTg                                                                          <  2:363977/101‑1 (MQ=255)
                       tCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTg                                                                          <  1:3259574/101‑1 (MQ=255)
                       gcGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTg                                                                          <  2:3252707/100‑1 (MQ=255)
                        cGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGa                                                                         <  2:2387411/101‑1 (MQ=255)
                         gCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGAt                                                                        >  2:7041560/1‑101 (MQ=255)
                         gCCACCTGCGTGGCAGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGAt                                                                        >  2:788600/1‑101 (MQ=255)
                          ccACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATc                                                                       <  1:3485014/101‑1 (MQ=255)
                          ccACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATc                                                                       <  2:4968776/101‑1 (MQ=255)
                           cACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCg                                                                      <  1:4930736/101‑1 (MQ=255)
                           cACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCg                                                                      <  1:4717445/101‑1 (MQ=255)
                           cACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCg                                                                      >  2:4717205/1‑101 (MQ=255)
                            cccTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGc                                                                     <  1:7059369/100‑1 (MQ=255)
                             ccTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCa                                                                    >  1:2447671/1‑101 (MQ=255)
                                 cGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCg                                                                >  1:144990/1‑101 (MQ=255)
                                 cGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCg                                                                >  1:2553576/1‑101 (MQ=255)
                                  gTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGt                                                               >  1:1021873/1‑101 (MQ=255)
                                   tGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTc                                                              >  2:2220420/1‑101 (MQ=255)
                                        gACAGCGTCGCCGCCGGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGagag                                                         <  1:3194031/101‑1 (MQ=255)
                                         aCAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgc                                                        <  1:6092869/101‑1 (MQ=255)
                                         aCAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgc                                                        >  2:3927473/1‑101 (MQ=255)
                                          cAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgcg                                                       >  1:5549230/1‑101 (MQ=255)
                                           aGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgcgc                                                      >  2:6000149/1‑101 (MQ=255)
                                           aGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAgcgc                                                      <  1:5374845/101‑1 (MQ=255)
                                              gTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCgcggc                                                   <  1:6150715/101‑1 (MQ=255)
                                                cgccgcCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGa                                                 >  1:6445303/1‑101 (MQ=255)
                                                cgccgcCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGa                                                 >  2:1694546/1‑101 (MQ=255)
                                                  ccgccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGaaa                                               <  2:6435157/101‑1 (MQ=255)
                                                     ccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATcc                                            <  2:2525755/101‑1 (MQ=255)
                                                     ccTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATcc                                            <  1:4567757/101‑1 (MQ=255)
                                                        gcgcCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATccgcc                                         <  1:418296/101‑1 (MQ=255)
                                                            cGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCt                                     >  2:1553366/1‑101 (MQ=255)
                                                             gTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTg                                    >  2:6143274/1‑101 (MQ=255)
                                                              tGGTCAGGCCGTCAATCTGCCCCGTGCTCAGCGTTGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGc                                   <  2:5786973/101‑1 (MQ=255)
                                                               ggTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCa                                  <  2:4024915/101‑1 (MQ=255)
                                                               ggTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCa                                  <  2:3029245/101‑1 (MQ=255)
                                                               ggTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCa                                  >  1:4336540/1‑101 (MQ=255)
                                                               ggTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCa                                  >  2:4261806/1‑101 (MQ=255)
                                                                gTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAg                                 >  2:3711321/1‑101 (MQ=255)
                                                                 tCAGGGCGTCTATCTGCCCCGTGCTCAGCGCGGCGAGCTGGGTCGAGCTCAGCCCCTTGATCGGAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAgc                                <  2:410322/101‑1 (MQ=255)
                                                                  cAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAgcg                               <  1:4873404/101‑1 (MQ=255)
                                                                     ggCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCgg                            >  1:375879/1‑101 (MQ=255)
                                                                     ggCGTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCgg                            <  1:6572792/101‑1 (MQ=255)
                                                                        gTCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGgaga                         >  1:3059756/1‑101 (MQ=255)
                                                                         tCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGAt                        <  2:6032132/101‑1 (MQ=255)
                                                                         tCGATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGAt                        <  1:2109022/101‑1 (MQ=255)
                                                                          cGATATGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATc                       >  2:2885682/1‑101 (MQ=255)
                                                                           gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                      <  2:3965028/101‑1 (MQ=255)
                                                                           gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                      <  2:270337/101‑1 (MQ=255)
                                                                           gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                      <  2:1480001/101‑1 (MQ=255)
                                                                           gATCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCt                      <  1:1793855/101‑1 (MQ=255)
                                                                             tCTGCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTgc                    <  1:135652/101‑1 (MQ=255)
                                                                                gCCCCGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGcc                 >  2:3828464/1‑101 (MQ=255)
                                                                                 ccccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCg                <  1:1629961/101‑1 (MQ=255)
                                                                                 ccccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCg                <  2:5860452/101‑1 (MQ=255)
                                                                                  cccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGt               <  1:1214523/101‑1 (MQ=255)
                                                                                   ccGTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTg              <  1:3811613/101‑1 (MQ=255)
                                                                                     gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt            >  2:6562150/1‑101 (MQ=255)
                                                                                     gTGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCt            <  2:6231526/101‑1 (MQ=255)
                                                                                      tGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCTCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTg           >  2:820747/1‑101 (MQ=255)
                                                                                      tGCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTg           <  2:6684447/101‑1 (MQ=255)
                                                                                       gCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGa          >  2:554131/1‑101 (MQ=255)
                                                                                       gCTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGa          >  2:1093264/1‑101 (MQ=255)
                                                                                        cTCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAg         <  1:3947764/101‑1 (MQ=255)
                                                                                         tCAGCGCGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAgc        <  1:5805220/101‑1 (MQ=255)
                                                                                            gcgcGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgcc     <  2:1393385/101‑1 (MQ=255)
                                                                                            gcgcGGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgcc     <  1:4080454/101‑1 (MQ=255)
                                                                                             cgcgGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgccg    <  2:190055/101‑1 (MQ=255)
                                                                                              gcgGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgccgc   >  2:3374959/1‑101 (MQ=255)
                                                                                              gcgGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgccgc   >  1:4790489/1‑101 (MQ=255)
                                                                                              gcgGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGcgccgc   >  1:2022211/1‑101 (MQ=255)
                                                                                               cgGCGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCgccgcc  <  1:2480455/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
ACCGATCTGGGCCGTCGACAGCGTCGCCACCTGCGTGGCCGACAGCGTCGCCGCCTGCGCCGTGGTCAGGGCGTCGATCTGCCCCGTGCTCAGCGCGGTGATCTGGGTCGAGCTCAGCCCCTTGATCGCAGCCGTCGAGAGCGCGGCGAAATCCGCCGTCTGCAGCGCGGAGATCTGCGCCGTGCTGAGCGCCGCC  >  NC_014034/2232111‑2232306

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG