Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,316 A→C 3.6% intergenic (‑112/‑193) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3160A→C 3.6% 22.8 0.0 ‑0.5392intergenic (‑112/‑193)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (7/7):  ref base (182/195):  total (190/202)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.10e-01

Only 100 of 407 total aligned reads displayed.

CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACG  >  NC_014034/2249217‑2249415
                                                                                                      |                                                                                                            
cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                                              <  1:4690510/101‑1 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                            >  1:1003442/1‑101 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                            >  2:551654/1‑101 (MQ=255)
  tccgatctCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                            <  2:5219635/94‑1 (MQ=38)
    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                                          <  1:5361363/101‑1 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCCCCCGGGGCAAGAAGGGGCCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGATGCAAGa                                                                                                       <  2:6235548/101‑1 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                       >  1:684217/1‑101 (MQ=255)
        ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                                      >  1:595637/1‑101 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                    <  1:663938/101‑1 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                    >  1:2568985/1‑101 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                    >  2:464063/1‑101 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                    <  1:1677176/101‑1 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                    <  2:5848908/101‑1 (MQ=255)
           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                   >  2:4434276/1‑101 (MQ=255)
            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                               >  1:3387070/1‑101 (MQ=255)
            tGAGATGCACAGATACCGCAGCCTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                               <  2:2056521/101‑1 (MQ=255)
             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                              >  2:6044022/1‑101 (MQ=255)
                gtGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                         <  2:5575516/100‑1 (MQ=255)
                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                        <  2:1299321/101‑1 (MQ=255)
                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGc                                                                                       <  2:4842043/101‑1 (MQ=255)
                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTGGAGGCAAGATGAA...GGGG..GGc                                                                                       >  1:4390792/1‑101 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                      <  2:6931060/101‑1 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                      >  1:5213294/1‑101 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                      >  2:84477/1‑101 (MQ=255)
                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                     <  2:5280813/101‑1 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                    <  1:672067/101‑1 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                    <  1:5867733/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                   <  1:4127248/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                   <  1:38456/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                   >  2:6791554/1‑101 (MQ=255)
                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                  >  1:1524407/1‑101 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCag                                                                                 >  2:4180792/1‑100 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                 >  1:402652/1‑101 (MQ=255)
                          aCCGCAGCTTGGGCACCCGGGGCAGGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCt                                                                               >  2:3406726/1‑101 (MQ=255)
                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                             <  1:4523314/101‑1 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                          <  2:456697/101‑1 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                          >  1:2310053/1‑101 (MQ=255)
                                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                         >  1:6156798/1‑101 (MQ=255)
                                 cgtGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                        <  2:735009/99‑1 (MQ=255)
                                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                      >  2:1649286/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                     >  1:4807769/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                     <  1:926334/101‑1 (MQ=255)
                                     ggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGCAAata                                                                    <  2:3521455/101‑1 (MQ=255)
                                          ccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                               <  2:1202602/101‑1 (MQ=255)
                                          ccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                               <  2:1173185/101‑1 (MQ=255)
                                           cGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                              <  1:6911051/101‑1 (MQ=255)
                                            ggggCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                             >  2:2198174/1‑101 (MQ=255)
                                              ggCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATAGTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                           >  1:4204837/1‑101 (MQ=255)
                                               gCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                          <  2:1056906/101‑1 (MQ=255)
                                                cAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                         >  2:641707/1‑100 (MQ=255)
                                                  agaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                       >  2:2681487/1‑101 (MQ=255)
                                                   gaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                      >  1:547608/1‑101 (MQ=255)
                                                    gagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                     <  1:641727/100‑1 (MQ=255)
                                                    aagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                     >  2:4112686/1‑101 (MQ=255)
                                                    aagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                     <  2:1537081/101‑1 (MQ=255)
                                                     agGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAATg                                                    >  2:6160690/1‑101 (MQ=38)
                                                     agGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                    >  2:5702431/1‑101 (MQ=255)
                                                       ggggCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                  >  2:17517/1‑101 (MQ=255)
                                                       ggggCTCTG.CCCACGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                  <  1:7070490/101‑1 (MQ=255)
                                                        gggCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                                                 >  2:293063/1‑101 (MQ=255)
                                                            tctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...Gc                                          <  2:1131284/96‑1 (MQ=255)
                                                            ctgg.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...Gcc                                         >  1:5598807/4‑101 (MQ=255)
                                                             ctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...Gcc                                         >  1:3296881/5‑101 (MQ=255)
                                                              tgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCa                                        <  1:5088714/98‑1 (MQ=255)
                                                               gaccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCag                                       >  2:2783494/3‑101 (MQ=255)
                                                                cccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCaga                                      <  1:5260297/100‑1 (MQ=255)
                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAggggg                                 <  1:1855755/101‑1 (MQ=255)
                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAggggg                                 >  2:3222722/1‑101 (MQ=255)
                                                                      gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAggggg                                 <  1:3627731/101‑1 (MQ=255)
                                                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAgggggg                                >  1:6248853/1‑101 (MQ=255)
                                                                        cgcgtGCCGAGATT.GCCCCGGGATATTTGAGTGAAGATGAC...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGc                               <  2:1373054/99‑1 (MQ=25)
                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGc                               <  1:1153704/101‑1 (MQ=255)
                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGc                               <  1:3041851/101‑1 (MQ=255)
                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGc                               >  1:2832057/1‑101 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCa                              >  2:5024477/1‑101 (MQ=255)
                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAg                             >  1:603282/1‑101 (MQ=255)
                                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAgc                            >  1:1394377/1‑101 (MQ=255)
                                                                           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAgc                            >  1:2098610/1‑101 (MQ=255)
                                                                            caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAA...GCCAGAGGGGGGc                               >  1:1071622/6‑101 (MQ=18)
                                                                             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAgcg.c                         >  1:346240/1‑101 (MQ=255)
                                                                               gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAA...GCCAGAGGGGGGCAgc                            <  1:3722323/99‑1 (MQ=18)
                                                                                gggGCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.cccc                      <  2:2636775/101‑1 (MQ=255)
                                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.ccccc                     <  1:2951426/101‑1 (MQ=255)
                                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.ccccc                     <  1:1228434/101‑1 (MQ=255)
                                                                                  gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCTAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.cccccc                    <  2:5441827/101‑2 (MQ=255)
                                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.cccccctt                  <  1:2198269/101‑4 (MQ=255)
                                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.cccccctt                  <  2:6863426/101‑4 (MQ=255)
                                                                                     t.CCCCCGGGATATCTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.ccccccttc                 <  2:4337424/101‑5 (MQ=255)
                                                                                       cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.ccccccttcg                <  1:2021198/101‑6 (MQ=255)
                                                                                        ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTcgc                <  2:3106532/101‑1 (MQ=255)
                                                                                          ccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgcc                 <  1:3095373/101‑4 (MQ=0)
                                                                                          ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCgcgg              <  1:3788549/101‑1 (MQ=255)
                                                                                           cGGGATNNGGGAGGNANNNGGAN...GGGN..NNNNGTCATCTTGCCGAAAAGAGCCCGGGGGGGGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCgcggg             <  1:4935326/101‑1 (MQ=16)
                                                                                            gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATAGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc            >  2:2394347/1‑101 (MQ=255)
                                                                                             ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCCGCGCCCCCCTTCgcgggcg           >  2:3008515/1‑101 (MQ=255)
                                                                                             ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcg           >  2:191590/1‑101 (MQ=255)
                                                                                                  tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc      >  2:375649/1‑101 (MQ=255)
                                                                                                  tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc      <  2:3958137/101‑1 (MQ=255)
                                                                                                   ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg     <  2:6668292/101‑1 (MQ=255)
                                                                                                      aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcg  >  2:5770225/1‑101 (MQ=255)
                                                                                                      |                                                                                                            
CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCA...GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACG  >  NC_014034/2249217‑2249415

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG