Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,317 G→A 1.9% intergenic (‑113/‑192) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3170G→A 1.9% 8.4 ‑0.3 ‑0.0390intergenic (‑113/‑192)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/5):  ref base (185/196):  total (188/202)
Fisher's exact test strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.17e-01

Only 100 of 406 total aligned reads displayed.

ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416
                                                                                                     |                                                                                                              
 ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                               >  1:1003442/1‑101 (MQ=255)
 tccgatctCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                               <  2:5219635/94‑1 (MQ=38)
  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                              <  1:4918142/101‑1 (MQ=255)
   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGATT.CCCCCGGGATATTTGAGGCa                                                                                                             <  1:4793157/101‑1 (MQ=255)
   ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                                             <  1:5361363/101‑1 (MQ=255)
     tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                           <  1:4010205/101‑1 (MQ=255)
      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCCCCCGGGGCAAGAAGGGGCCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGATGCAAGa                                                                                                          <  2:6235548/101‑1 (MQ=255)
      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                          <  2:3483539/101‑1 (MQ=255)
       ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                                         >  1:1236551/1‑101 (MQ=255)
       ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                                         >  1:595637/1‑101 (MQ=255)
       ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAt                                                                                                         <  2:2553641/101‑1 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                       >  1:2568985/1‑101 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                       >  2:464063/1‑101 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                       <  2:5848908/101‑1 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                       <  1:1677176/101‑1 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                      >  2:4434276/1‑101 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                                      >  1:3201834/1‑101 (MQ=255)
           tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                                  >  1:6910931/1‑101 (MQ=255)
            gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                                                 >  2:6044022/1‑101 (MQ=255)
               gtGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                            <  2:5575516/100‑1 (MQ=255)
                tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                           <  2:1299321/101‑1 (MQ=255)
                 gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGc                                                                                          <  2:4842043/101‑1 (MQ=255)
                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                         <  2:6931060/101‑1 (MQ=255)
                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCt                                                                                         >  2:84477/1‑101 (MQ=255)
                   acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                        <  2:3055129/101‑1 (MQ=255)
                   acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                        <  2:5280813/101‑1 (MQ=255)
                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                       <  2:552959/101‑1 (MQ=255)
                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCttt                                                                                       <  1:5867733/101‑1 (MQ=255)
                     ctaTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                      <  1:4180987/99‑1 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                      >  2:6791554/1‑101 (MQ=255)
                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                     >  1:1524407/1‑101 (MQ=255)
                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCag                                                                                    >  2:4180792/1‑100 (MQ=255)
                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                    <  1:4117337/101‑1 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                             <  1:378241/101‑1 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                             <  2:456697/101‑1 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                             >  1:2310053/1‑101 (MQ=255)
                               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                            >  1:6156798/1‑101 (MQ=255)
                                cgtGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                           <  2:735009/99‑1 (MQ=255)
                                 ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                          >  2:355352/1‑101 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                         >  2:1649286/1‑101 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTAATCTTGCCGagaa                                                                         >  2:3300747/1‑101 (MQ=38)
                                   gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                        >  1:4654927/1‑101 (MQ=255)
                                    ggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                       <  1:6618084/101‑1 (MQ=255)
                                      cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                     >  1:382347/1‑101 (MQ=255)
                                        cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                   <  2:2672973/101‑1 (MQ=255)
                                         ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                  >  1:300406/1‑101 (MQ=255)
                                         ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                  <  2:1202602/101‑1 (MQ=255)
                                         ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                  <  2:1173185/101‑1 (MQ=255)
                                         ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                  <  1:6379156/101‑1 (MQ=255)
                                              gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                             <  2:1056906/101‑1 (MQ=255)
                                               cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                            >  2:641707/1‑100 (MQ=255)
                                               cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                            >  1:5161729/1‑100 (MQ=255)
                                                aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                           >  2:2169379/1‑101 (MQ=255)
                                                  gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                         >  1:547608/1‑101 (MQ=255)
                                                   aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                       >  1:915172/1‑101 (MQ=255)
                                                   aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                       <  2:1537081/101‑1 (MQ=255)
                                                   aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                       >  2:4112686/1‑101 (MQ=255)
                                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.Tg                                                      >  2:6160690/1‑101 (MQ=38)
                                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                      >  2:5702431/1‑101 (MQ=255)
                                                    agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGAGGGGTGAA.Tg                                                      >  1:5075584/1‑101 (MQ=255)
                                                       gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                   >  2:293063/1‑101 (MQ=255)
                                                          ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...g                                             >  1:4492496/1‑101 (MQ=255)
                                                          ctctGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...g                                             <  1:3419422/101‑1 (MQ=255)
                                                           tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gc                                            <  2:1131284/96‑1 (MQ=255)
                                                           tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA...Gc                                            >  1:4337646/6‑101 (MQ=255)
                                                           ctgg.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gcc                                           >  1:5598807/4‑101 (MQ=255)
                                                              gaccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCag                                         >  2:2783494/3‑101 (MQ=255)
                                                               cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCaga                                        <  1:5260297/100‑1 (MQ=255)
                                                                ccccCTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCagag                                       <  2:2304837/101‑1 (MQ=255)
                                                                    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAg.gggg                                  <  1:1855755/101‑1 (MQ=255)
                                                                    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAg.gggg                                  >  1:1163344/1‑101 (MQ=255)
                                                                     tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAg.ggggg                                 >  1:6248853/1‑101 (MQ=255)
                                                                      cgcgtGCCGAGATT.GCCCCGGGATATTTGAGTGAAGATGAC...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGc                                <  2:1373054/99‑1 (MQ=25)
                                                                      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGc                                >  1:2832057/1‑101 (MQ=255)
                                                                       ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCa                               >  2:5024477/1‑101 (MQ=255)
                                                                        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCGTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAg                              >  1:3616988/1‑101 (MQ=255)
                                                                        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAg                              >  1:603282/1‑101 (MQ=255)
                                                                         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgc                             >  1:2098610/1‑101 (MQ=255)
                                                                         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgc                             >  1:1394377/1‑101 (MQ=255)
                                                                          tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgcg                            >  1:3719900/1‑101 (MQ=255)
                                                                          caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA...GCCAGAG.GGGGGc                                >  1:1071622/6‑101 (MQ=18)
                                                                          caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAAGATCCCGGGGGGTGAA.TGGCGAA...GCCAGAG.GGGGGc                                >  1:3373146/6‑101 (MQ=18)
                                                                            ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cc                         <  2:1744695/101‑1 (MQ=255)
                                                                               ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccc                      >  1:3079255/1‑101 (MQ=255)
                                                                                gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cccccc                     <  1:294354/101‑2 (MQ=255)
                                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cccccctt                   <  2:6863426/101‑4 (MQ=255)
                                                                                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cccccctt                   <  1:2198269/101‑4 (MQ=255)
                                                                                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccccttc                  >  1:6699743/1‑97 (MQ=255)
                                                                                   t.CCCCCGGGATATCTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccccttc                  <  2:4337424/101‑5 (MQ=255)
                                                                                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccccttcg                 <  1:2021198/101‑6 (MQ=255)
                                                                                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccccttcg                 >  2:3151444/1‑96 (MQ=255)
                                                                                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.CCCCCtgccc                 >  2:2012580/1‑97 (MQ=0)
                                                                                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCCAAAGGCCA.AGAGGGGGCAGCG.CCCCCTTccc                 <  1:6832091/101‑3 (MQ=0)
                                                                                                tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCTCGGGGGGGGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc       >  1:7068660/1‑101 (MQ=37)
                                                                                                tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc       <  2:3958137/101‑1 (MQ=255)
                                                                                                tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc       >  2:375649/1‑101 (MQ=255)
                                                                                                 ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg      <  2:6668292/101‑1 (MQ=255)
                                                                                                   gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc    >  2:5442851/1‑101 (MQ=255)
                                                                                                    aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcg   >  2:5770225/1‑101 (MQ=255)
                                                                                                    aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcg   >  2:1909981/1‑101 (MQ=255)
                                                                                                     |                                                                                                              
ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG