Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,347 G→C 3.9% intergenic (‑143/‑162) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3470G→C 3.9% 24.5 0.0 ‑0.7387intergenic (‑143/‑162)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (8/7):  ref base (198/173):  total (206/181)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.15e-01

Only 100 of 407 total aligned reads displayed.

AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446
                                                                                                           |                                                                                                          
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                                            >  2:1906171/1‑101 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                          >  2:6910697/1‑101 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                          <  1:1312018/101‑1 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                          >  1:4227783/1‑101 (MQ=255)
   ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                                         >  2:4437105/1‑101 (MQ=255)
    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                                        >  2:1649286/1‑101 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                       <  1:1715073/101‑1 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                       <  1:4819503/101‑1 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                       <  2:2423721/101‑1 (MQ=255)
       gCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                                     <  2:1997101/101‑1 (MQ=255)
       gCACCCGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                                     <  1:6993560/101‑1 (MQ=255)
          cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                                  <  1:5925036/101‑1 (MQ=255)
          cccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                                  <  2:2672973/101‑1 (MQ=255)
           ccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                 >  1:300406/1‑101 (MQ=255)
           ccGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                 <  2:1202602/101‑1 (MQ=255)
            cGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                                <  2:461277/101‑1 (MQ=255)
            cGGGGCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                                >  2:3840217/1‑101 (MQ=255)
             ggggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                               >  2:4336443/1‑101 (MQ=255)
               ggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                                             <  1:4336665/101‑1 (MQ=255)
               ggCAAGAAGGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                                             >  1:6303693/1‑101 (MQ=255)
                  aaggagGGGGCCCTG.CCCCAATCCCTTGCCCGGACT.CCCCCTGTATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTTCCGAAAATATCCCGGGGGGTg                                                                                          <  2:677531/101‑1 (MQ=25)
                    gaagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                        <  1:3794454/101‑1 (MQ=255)
                     aagGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                      <  2:1537081/101‑1 (MQ=255)
                      agGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.Tg                                                                                     >  2:6160690/1‑101 (MQ=38)
                      agGGGGCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                     >  2:5702431/1‑101 (MQ=255)
                        agggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                   <  1:5466088/100‑1 (MQ=255)
                         gggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                  >  2:293063/1‑101 (MQ=255)
                         gggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                  <  1:1009599/101‑1 (MQ=255)
                          ggCTCTGCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                 >  2:6075470/1‑101 (MQ=255)
                              ctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gcc                                                                          >  1:3296881/5‑101 (MQ=255)
                              ctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gcc                                                                          <  2:5213029/97‑1 (MQ=255)
                              ctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gcc                                                                          <  2:5809293/97‑1 (MQ=255)
                              ctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gcc                                                                          <  1:84478/97‑1 (MQ=255)
                               tgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCa                                                                         <  1:5088714/98‑1 (MQ=255)
                                 ccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCaga                                                                       <  2:3161145/100‑1 (MQ=255)
                                        tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAg.ggggg                                                                <  2:1059368/101‑1 (MQ=255)
                                         cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGc                                                               >  1:1399096/1‑101 (MQ=255)
                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgc                                                            >  2:1023740/1‑101 (MQ=255)
                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgc                                                            >  2:6251473/1‑101 (MQ=255)
                                             caagcggACTCCCCCCGGGATATTTGGAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA...GCCAGAg.ggggga                                                               >  1:4752660/6‑100 (MQ=18)
                                             caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAAGATCCCGGGGGGTGAA.TGGCGAA...GCCAGAG.GGGGGc                                                               >  1:3373146/6‑101 (MQ=18)
                                              gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgcg.c                                                         >  2:4909331/1‑101 (MQ=255)
                                              gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGAGAA...GCCAGAG.GGGGGCAgcg.c                                                         >  1:5228563/1‑101 (MQ=255)
                                                cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccc                                                       >  1:2353522/1‑101 (MQ=255)
                                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA...GCCAGAG.GGGGGCAgcg.c                                                         <  1:6124116/101‑1 (MQ=17)
                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccc                                                     >  1:3638035/1‑101 (MQ=255)
                                                   gactccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA...GCCAGAG.GGGGGCAGCG.cc                                                        <  1:202820/96‑1 (MQ=17)
                                                   gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCTAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cccccc                                                    <  2:5441827/101‑2 (MQ=255)
                                                    aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTTATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cccccct                                                   <  1:6672530/101‑3 (MQ=255)
                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cccccctt                                                  <  1:2198269/101‑4 (MQ=255)
                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccccttc                                                 <  1:6739474/101‑5 (MQ=255)
                                                      cgcccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.cccccctt                                                  <  2:4144243/99‑4 (MQ=255)
                                                        cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.ccccccttcg                                                <  2:2863072/101‑6 (MQ=255)
                                                           ccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCCAAAGGCCAAGA.GGGGGCAGCG.CCCCC.tgcc                                                <  1:3095373/101‑4 (MQ=0)
                                                            cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.CCCCC.tgccc                                               >  1:2695792/1‑97 (MQ=0)
                                                            cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.CCCCC.tgccc                                               >  1:1102536/1‑97 (MQ=0)
                                                            cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCCAAAGGCCA.AGAGGGGGCAGCG.CCCCC.TTccc                                               <  1:6832091/101‑3 (MQ=0)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCgcgggc                                           >  2:1144534/1‑101 (MQ=255)
                                                             gggATATTTGAGCAAAGTTGAA...GGGG..GGCTTTAATCTTGCCGAAAAAATCCCGGGGGGGGAA.TGGGGCA...GCCAGAG.GGGGGCGGCGCCCCCC.TTCgcgggc                                           >  2:3741255/1‑101 (MQ=255)
                                                              ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCCGCGCCCCCC.TTCgcgggcg                                          >  2:3008515/1‑101 (MQ=255)
                                                               gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCgcgggcgg                                         <  1:4112880/101‑1 (MQ=255)
                                                               gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCgcgggcgg                                         >  2:742353/1‑101 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCcg                                      >  1:3734564/1‑101 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCcg                                      >  1:533331/1‑101 (MQ=255)
                                                                   tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgc                                     <  2:3958137/101‑1 (MQ=255)
                                                                   tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgc                                     >  2:221725/1‑101 (MQ=255)
                                                                    ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCcgcg                                    <  2:6668292/101‑1 (MQ=255)
                                                                        ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcgc                                <  1:2935905/101‑1 (MQ=255)
                                                                            aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACgcgcgc                            >  1:6603896/1‑101 (MQ=255)
                                                                             gaagAA...GGGG..GACTTTCATCTTGGCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGAcgcgcgac                           <  2:5350376/98‑3 (MQ=25)
                                                                             gATGGA...GGGG..GGCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc                           <  1:1593521/101‑1 (MQ=37)
                                                                             gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGGGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc                           >  1:715783/1‑101 (MQ=255)
                                                                             gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgcc                           <  2:552067/101‑1 (MQ=255)
                                                                              aTGAA...GGGG..TGCGTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGcgccg                          <  2:1376315/101‑1 (MQ=25)
                                                                              aTGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGagccg                          >  2:840316/1‑101 (MQ=37)
                                                                                gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCa                        >  2:6834844/1‑101 (MQ=255)
                                                                                      gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa                     <  1:2146792/101‑1 (MQ=255)
                                                                                      gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa                     <  1:6048378/101‑1 (MQ=255)
                                                                                      gggg..gcCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACa                     <  2:5891695/101‑1 (MQ=255)
                                                                                        gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                   >  2:3082247/1‑101 (MQ=255)
                                                                                        gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.CCCCCATTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                   <  1:5007893/101‑1 (MQ=255)
                                                                                         g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                  >  1:3438974/1‑101 (MQ=255)
                                                                                            ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                 <  2:2100716/101‑1 (MQ=255)
                                                                                               tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCggg              >  1:3938455/1‑99 (MQ=255)
                                                                                               tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              <  2:2770006/101‑1 (MQ=255)
                                                                                               tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              >  1:3613464/1‑101 (MQ=255)
                                                                                                 tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc            >  2:3498982/1‑101 (MQ=255)
                                                                                                 tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc            >  2:2562612/1‑101 (MQ=255)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           >  2:4388175/1‑101 (MQ=255)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           >  1:6219780/1‑101 (MQ=255)
                                                                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc          <  1:1185207/101‑1 (MQ=255)
                                                                                                    tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt         <  2:6487232/101‑1 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        >  2:7080395/1‑101 (MQ=255)
                                                                                                     cTTACCGAAGTTATGCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        <  1:2783596/101‑1 (MQ=37)
                                                                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTGGCGGGCGGCCGCGACGCGCGCCGCAACAGTTGGTGCCCCttt       >  1:5565268/1‑101 (MQ=25)
                                                                                                        gcagAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt     <  1:4480838/98‑1 (MQ=255)
                                                                                                          cGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa   <  2:1316993/101‑1 (MQ=255)
                                                                                                          cGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa   >  2:4054470/1‑101 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt  <  1:5751657/101‑1 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCCCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt  >  1:1861232/1‑101 (MQ=255)
                                                                                                           |                                                                                                          
AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.CCCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG