Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,348 A→G 4.0% intergenic (‑144/‑161) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3480A→G 4.0% 25.4 0.0 ‑0.8379intergenic (‑144/‑161)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (8/7):  ref base (190/172):  total (198/181)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.41e-01

Only 100 of 406 total aligned reads displayed.

GCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATT  >  NC_014034/2249249‑2249447
                                                                                                            |                                                                                                       
 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                        >  1:4227783/1‑101 (MQ=255)
 cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                        >  2:6910697/1‑101 (MQ=255)
  ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                                       >  2:4437105/1‑101 (MQ=255)
   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                                      >  2:1649286/1‑101 (MQ=255)
    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                     <  2:2423721/101‑1 (MQ=255)
      gCACCCGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                                                   <  2:1997101/101‑1 (MQ=255)
       cACCCGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                                  <  1:2230758/101‑1 (MQ=255)
         cccGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                                <  2:2672973/101‑1 (MQ=255)
         cccGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                                                <  1:5925036/101‑1 (MQ=255)
          ccGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                               <  2:1202602/101‑1 (MQ=255)
           cGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                              >  2:3840217/1‑101 (MQ=255)
           cGGGGCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                              <  2:561713/101‑1 (MQ=255)
            ggggCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                             >  2:4336443/1‑101 (MQ=255)
              ggCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                                           <  1:4336665/101‑1 (MQ=255)
              ggCAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                                           >  1:6303693/1‑101 (MQ=255)
                cAAGAAGGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggggg                                                                                         >  1:5161729/1‑100 (MQ=255)
                 aaggagGGGGCCCTG.CCC.CAATCCCTTGCCCGGACT.CCCCCTGTATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTTCCGAAAATATCCCGGGGGGTg                                                                                        <  2:677531/101‑1 (MQ=25)
                  agaagGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                       >  2:2935796/1‑101 (MQ=255)
                  agaagGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                       <  1:1277483/101‑1 (MQ=255)
                   gaagGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                      <  1:3794454/101‑1 (MQ=255)
                    aagGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                    <  2:1537081/101‑1 (MQ=255)
                    aagGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                    <  1:6978797/101‑1 (MQ=255)
                     agGGGGCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.Tg                                                                                   >  2:6160690/1‑101 (MQ=38)
                      gggggCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                                  >  2:5819885/1‑101 (MQ=255)
                       ggggCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                 >  2:17517/1‑101 (MQ=255)
                       ggggCTCTG.CCCAC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                 <  1:7070490/101‑1 (MQ=255)
                       agggCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                 <  1:5466088/100‑1 (MQ=255)
                        gggCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                <  1:1009599/101‑1 (MQ=255)
                         ggCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                               >  2:6075470/1‑101 (MQ=255)
                          gCTCTGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                              >  1:6641539/1‑101 (MQ=255)
                           ctctGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                           <  1:3419422/101‑1 (MQ=255)
                           ctctGCCCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..g                                                                           >  2:5384173/1‑101 (MQ=255)
                                   c.c.gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                   <  1:1987405/101‑1 (MQ=255)
                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 <  1:3627731/101‑1 (MQ=255)
                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 <  1:1855755/101‑1 (MQ=255)
                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                 >  1:1163344/1‑101 (MQ=255)
                                        tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                <  2:1059368/101‑1 (MQ=255)
                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                              >  2:5024477/1‑101 (MQ=255)
                                           cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                             <  1:1649347/101‑1 (MQ=255)
                                           cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.AGGCGCA..GCCAGAGGGGGGCAg                                                             >  1:497490/1‑101 (MQ=255)
                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                            >  2:1023740/1‑101 (MQ=255)
                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                            >  2:6251473/1‑101 (MQ=255)
                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                            >  1:1394377/1‑101 (MQ=255)
                                                gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgc                                                            <  1:3722323/99‑1 (MQ=18)
                                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgcg.c                                                         <  1:6124116/101‑1 (MQ=17)
                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccc                                                     >  1:3079255/1‑101 (MQ=255)
                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccc                                                     <  1:2951426/101‑1 (MQ=255)
                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccc                                                     <  2:5238031/101‑1 (MQ=255)
                                                      cgcccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                                  <  2:4144243/99‑4 (MQ=255)
                                                       ccccccGGGATATGTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.ccccc                                                     <  2:4787575/100‑1 (MQ=2)
                                                        cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttcg                                                <  2:2863072/101‑6 (MQ=255)
                                                         ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTcgc                                                <  2:3106532/101‑1 (MQ=255)
                                                           ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgg                                              <  1:208961/101‑1 (MQ=255)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc                                            <  1:355356/101‑1 (MQ=255)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc                                            <  1:2171587/101‑1 (MQ=255)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc                                            >  2:1144534/1‑101 (MQ=255)
                                                             gggATATTTGAGCAAAGTTGAA...GGGG..GGCTTTAATCTTGCCGAAAAAATCCCGGGGGGGGAA.TGGGGCA..GCCAGAGGGGGGCGGCGCCCCCCTTCgcgggc                                            >  2:3741255/1‑101 (MQ=255)
                                                               gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcgg                                          <  1:4112880/101‑1 (MQ=255)
                                                               gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcgg                                          >  2:742353/1‑101 (MQ=255)
                                                                atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGGA.TGGCGCA..GCCAGAGGGGGGCAGGGCCCCCCTTCGCCggcggc                                         >  1:6513119/1‑101 (MQ=38)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcg                                       >  1:481809/1‑101 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcg                                       <  1:2418644/101‑1 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcg                                       >  1:533331/1‑101 (MQ=255)
                                                                   tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc                                      >  2:221725/1‑101 (MQ=255)
                                                                   tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgc                                      <  2:3958137/101‑1 (MQ=255)
                                                                    ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg                                     <  2:6668292/101‑1 (MQ=255)
                                                                       aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcg                                  <  2:3179761/101‑1 (MQ=255)
                                                                       aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcg                                  >  2:5770225/1‑101 (MQ=255)
                                                                             gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                            <  1:6791908/101‑1 (MQ=255)
                                                                              aTGAA...GGGG..TGCGTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                           <  2:1376315/101‑1 (MQ=25)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccc                           >  2:3047794/1‑100 (MQ=255)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGAGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                           >  1:1486584/1‑101 (MQ=255)
                                                                              aTGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGagccg                           >  2:840316/1‑101 (MQ=37)
                                                                                gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                         >  2:6834844/1‑101 (MQ=255)
                                                                                      gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                      <  2:3507362/101‑1 (MQ=255)
                                                                                      gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                      <  1:6048378/101‑1 (MQ=255)
                                                                                       ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                     >  1:673427/1‑101 (MQ=255)
                                                                                            ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                  <  2:2100716/101‑1 (MQ=255)
                                                                                             gCTTTCATCTTGACGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                 >  1:5080654/1‑101 (MQ=255)
                                                                                              cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                <  1:293067/101‑1 (MQ=255)
                                                                                               tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCggg               >  1:3938455/1‑99 (MQ=255)
                                                                                               tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg               <  2:2770006/101‑1 (MQ=255)
                                                                                                 tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc             >  2:2562612/1‑101 (MQ=255)
                                                                                                 tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc             >  2:5474758/1‑101 (MQ=255)
                                                                                                 tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc             <  2:5934343/101‑1 (MQ=255)
                                                                                                 tCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCACCAGGTCGTGcc             >  1:773936/1‑101 (MQ=37)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc            >  1:6219780/1‑101 (MQ=255)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc            >  2:4388175/1‑101 (MQ=255)
                                                                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc           <  1:2759726/101‑1 (MQ=255)
                                                                                                    tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGGCGCGACGCGCGCCGCCACAGGTCGTCCCCCt          >  1:4716913/1‑101 (MQ=25)
                                                                                                    tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt          <  2:6487232/101‑1 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt         >  2:7080395/1‑101 (MQ=255)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGCCCCtt         >  1:3269017/1‑101 (MQ=255)
                                                                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTGGCGGGCGGCCGCGACGCGCGCCGCAACAGTTGGTGCCCCttt        >  1:5565268/1‑101 (MQ=25)
                                                                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt        <  1:3351043/101‑1 (MQ=255)
                                                                                                        gcagAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt      <  1:4480838/98‑1 (MQ=255)
                                                                                                          cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa    <  2:1316993/101‑1 (MQ=255)
                                                                                                          cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa    >  2:4054470/1‑101 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt   <  1:5751657/101‑1 (MQ=255)
                                                                                                            aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt  >  1:1091367/1‑101 (MQ=255)
                                                                                                            |                                                                                                       
GCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.C.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATT  >  NC_014034/2249249‑2249447

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG