Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,359 G→A 2.3% intergenic (‑155/‑150) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3590G→A 2.3% 9.6 ‑1.1 ‑0.3371intergenic (‑155/‑150)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (7/3):  ref base (189/172):  total (196/175)
Fisher's exact test strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.37e-01

Only 100 of 408 total aligned reads displayed.

CGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249260‑2249458
                                                                                                    |                                                                                                     
 ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                                                                                      >  2:2198174/1‑101 (MQ=255)
         aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                                              <  1:6978797/101‑1 (MQ=255)
         aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                                                              >  2:4112686/1‑101 (MQ=255)
          agGGGGCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                                                                             <  2:2666785/101‑1 (MQ=255)
            ggggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                                           >  2:17517/1‑101 (MQ=255)
            agggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                                                           <  1:5466088/100‑1 (MQ=255)
             gggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                                                                                          >  1:2913370/1‑101 (MQ=255)
             gggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                                                                                          <  1:1009599/101‑1 (MQ=255)
                ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                                                                       >  2:440226/1‑101 (MQ=255)
                ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAg                                                                                       >  2:5384173/1‑101 (MQ=255)
                  ctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                                                     <  2:5213029/97‑1 (MQ=255)
                  ctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                                                                     <  2:5809293/97‑1 (MQ=255)
                    gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                                                   >  2:2925381/3‑101 (MQ=255)
                    gcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                                                                                   >  1:1891169/3‑101 (MQ=255)
                     cccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                                                                                  <  2:3161145/100‑1 (MQ=255)
                         cctcccggGCCGGGGCTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg                                                                              <  2:6499042/99‑1 (MQ=255)
                           tcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                                                                            >  1:6248853/1‑101 (MQ=255)
                            cgcgtGCCGAGATTGCCCCGGGATATTTGAGTGAAGATGACGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                                           <  2:1373054/99‑1 (MQ=25)
                            cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                                           <  1:1153704/101‑1 (MQ=255)
                            cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                                           <  1:3350564/101‑1 (MQ=255)
                            cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                                                           >  1:1399096/1‑101 (MQ=255)
                              cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAAGGCGCAGCCAGAGGGGGGCAg                                                                         >  1:497490/1‑101 (MQ=255)
                               gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc                                                                        >  1:2098610/1‑101 (MQ=255)
                                tGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg                                                                       <  2:684197/101‑1 (MQ=255)
                                 tccGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg.c                                                                     <  2:1031953/100‑1 (MQ=255)
                                 gCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg.c                                                                     >  2:4909331/1‑101 (MQ=255)
                                 gCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGAGAAGCCAGAGGGGGGCAgcg.c                                                                     >  1:5228563/1‑101 (MQ=255)
                                   cGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cc                                                                  >  1:316258/1‑101 (MQ=255)
                                    gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccc                                                                 >  1:5891997/1‑101 (MQ=255)
                                    gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccc                                                                 <  2:5556588/101‑1 (MQ=255)
                                    cggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccc                                                                 <  1:4473121/100‑1 (MQ=255)
                                     ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccc                                                                >  2:1249176/1‑101 (MQ=255)
                                     ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccc                                                                >  1:3079255/1‑101 (MQ=255)
                                     ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccc                                                                <  1:1228434/101‑1 (MQ=255)
                                     ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccc                                                                <  2:569645/101‑1 (MQ=255)
                                     ggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccc                                                                >  1:3638035/1‑101 (MQ=255)
                                       aCTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTTATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccccct                                                              <  1:6672530/101‑3 (MQ=255)
                                        cTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.ccccctt                                                             <  2:603262/101‑4 (MQ=255)
                                          cccccGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccccttcg                                                           <  1:2021198/101‑6 (MQ=255)
                                          cccccGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.c.cccccttcg                                                           >  1:4684026/1‑96 (MQ=255)
                                             ccGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcgg                                                         >  2:2854614/1‑101 (MQ=255)
                                               gggATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggc                                                       >  2:1144534/1‑101 (MQ=255)
                                               gggATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggc                                                       <  1:2171587/101‑1 (MQ=255)
                                                ggATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCCGCGCC.CCCCTTCgcgggcg                                                      >  2:3008515/1‑101 (MQ=255)
                                                ggATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggcg                                                      >  2:191590/1‑101 (MQ=255)
                                                 gATATTTGAGCCAAGATGAAGGGGGGCTTTCAGCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggcgg                                                     <  2:717367/101‑1 (MQ=25)
                                                  atatTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGGATGGCGCAGCCAGAGGGGGGCAGGGCC.CCCCTTCGCCggcggc                                                    >  1:6513119/1‑101 (MQ=38)
                                                   tatTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGcc                                                   <  2:4227588/101‑1 (MQ=255)
                                                   tatTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGcc                                                   >  2:5920788/1‑101 (MQ=255)
                                                    atTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                  >  1:481809/1‑101 (MQ=255)
                                                    atTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                  >  1:3734564/1‑101 (MQ=255)
                                                    atTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                  >  1:533331/1‑101 (MQ=255)
                                                     tttGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                 >  2:375649/1‑101 (MQ=255)
                                                        gAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAc                                              >  2:5442851/1‑101 (MQ=255)
                                                            cAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgcgc                                          >  2:4362411/1‑101 (MQ=255)
                                                               gATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGGGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       >  1:715783/1‑101 (MQ=255)
                                                               gATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       <  1:6791908/101‑1 (MQ=255)
                                                               gATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                       >  2:6157799/1‑101 (MQ=255)
                                                                aTGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                      >  2:833895/1‑101 (MQ=255)
                                                                aTGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGAGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                      >  1:1486584/1‑101 (MQ=255)
                                                                  gAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                    <  2:2063484/101‑1 (MQ=255)
                                                                   aaGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CACCCCTTCGCGGGCGGTCGCCACGGGCGCCGCaa                                   >  2:4028710/1‑101 (MQ=25)
                                                                     ggggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                 <  2:3507362/101‑1 (MQ=255)
                                                                      gggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                >  1:673427/1‑101 (MQ=255)
                                                                       ggggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                               >  2:3082247/1‑101 (MQ=255)
                                                                          gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                            >  2:6976694/1‑101 (MQ=255)
                                                                          gCTTTCATCTTGACGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                            >  1:5080654/1‑101 (MQ=255)
                                                                           cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                           >  1:4157106/1‑101 (MQ=255)
                                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          <  1:2949020/101‑1 (MQ=255)
                                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          <  2:2770006/101‑1 (MQ=255)
                                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                          >  1:3613464/1‑101 (MQ=255)
                                                                             ttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                         >  1:3966207/1‑101 (MQ=255)
                                                                             ttCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                         >  2:1522249/1‑101 (MQ=255)
                                                                              tCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                        >  2:6812935/1‑101 (MQ=255)
                                                                                aTCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                      <  1:6118507/101‑1 (MQ=255)
                                                                                aTCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                      >  2:3887808/1‑101 (MQ=255)
                                                                                  cTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                    >  1:2357460/1‑101 (MQ=255)
                                                                                  cTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                    >  1:4331909/1‑101 (MQ=255)
                                                                                  cTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                    >  1:1091007/1‑101 (MQ=255)
                                                                                   ttGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTGGCGGGCGGCCGCGACGCGCGCCGCAACAGTTGGTGCCCCttt                   >  1:5565268/1‑101 (MQ=25)
                                                                                   tggCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                   <  1:2718445/99‑1 (MQ=255)
                                                                                    tGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                  <  2:2533459/101‑1 (MQ=255)
                                                                                      ccGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                <  2:6699401/101‑1 (MQ=255)
                                                                                      ccGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                <  1:859924/101‑1 (MQ=255)
                                                                                        gAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              <  1:3838187/101‑1 (MQ=255)
                                                                                        gAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              <  1:5751657/101‑1 (MQ=255)
                                                                                        gAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt              >  2:1590847/1‑101 (MQ=255)
                                                                                          aaaTATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc            >  1:3486627/1‑101 (MQ=255)
                                                                                           aaTATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt           >  2:4712624/1‑101 (MQ=255)
                                                                                           aaTAGCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt           <  1:1354500/101‑1 (MQ=255)
                                                                                            atatCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg          >  2:1069444/1‑101 (MQ=255)
                                                                                              atgccGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc        <  2:2309958/98‑1 (MQ=255)
                                                                                              atCCCGGGGGGTGAATGGCGCAGCCAGAGGGGTGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc        <  1:3406850/101‑1 (MQ=255)
                                                                                              atCCCGGGGGGTGAATGGCGCAGCCAGAGGGGTGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc        >  1:1620050/1‑101 (MQ=255)
                                                                                              atCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc        >  2:325359/1‑101 (MQ=255)
                                                                                                cccGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc      <  1:6168075/101‑1 (MQ=255)
                                                                                                  cGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CGCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc    >  2:6228787/1‑101 (MQ=255)
                                                                                                   ggggggTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc   >  1:6042181/1‑101 (MQ=255)
                                                                                                   ggggggTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc   >  2:6345672/1‑101 (MQ=255)
                                                                                                    gggggTGAATGGCGCAGCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc  >  1:1764263/1‑101 (MQ=255)
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CGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCC  >  NC_014034/2249260‑2249458

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG