Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,372 G→C 2.1% intergenic (‑168/‑137) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3720G→C 2.1% 8.3 ‑0.3 ‑0.6383intergenic (‑168/‑137)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (196/179):  total (201/182)
Fisher's exact test strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.78e-01

Only 100 of 410 total aligned reads displayed.

GGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTG  >  NC_014034/2249273‑2249471
                                                                                                           |                                                                                                         
gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                                           <  1:1009599/101‑1 (MQ=255)
gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                                           >  2:293063/1‑101 (MQ=255)
  gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                                         >  1:6641539/1‑101 (MQ=255)
    tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gc                                                                                                    >  1:1436022/6‑101 (MQ=255)
       gaccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCag                                                                                                 >  2:2783494/3‑101 (MQ=255)
        cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCaga                                                                                                <  2:3161145/100‑1 (MQ=255)
          cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAg.g                                                                                             >  2:3838024/1‑101 (MQ=255)
             gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAg.gggg                                                                                          >  1:1163344/1‑101 (MQ=255)
               cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGc                                                                                        <  1:3350564/101‑1 (MQ=255)
                 cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAg                                                                                      <  2:6706884/101‑1 (MQ=255)
                 cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAg                                                                                      <  2:3073668/101‑1 (MQ=255)
                  gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgc                                                                                     >  2:1023740/1‑101 (MQ=255)
                   tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgcg                                                                                    >  1:3719900/1‑101 (MQ=255)
                   caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA...GCCAGAG.GGGGGc                                                                                        >  1:1071622/6‑101 (MQ=18)
                       gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccc                                                                              <  1:6323577/101‑1 (MQ=255)
                        ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccc                                                                             >  1:3079255/1‑101 (MQ=255)
                         gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCTAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccccc                                                                            <  2:5441827/101‑2 (MQ=255)
                          aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccccct                                                                           <  1:5280347/101‑3 (MQ=255)
                          aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccccct                                                                           <  2:68211/101‑3 (MQ=255)
                           cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TTGCGCA...GCCAGAG.GGGGGCAGCG.c.ccccctt                                                                          <  2:1314294/101‑4 (MQ=255)
                           cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccccctt                                                                          <  2:6863426/101‑4 (MQ=255)
                            t.CCCCCGGGATATCTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccccttc                                                                         <  2:4337424/101‑5 (MQ=255)
                            cgcccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccccctt                                                                          <  2:4144243/99‑4 (MQ=255)
                              cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccccttcg                                                                        <  2:2863072/101‑6 (MQ=255)
                                 ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCgcgg                                                                      <  1:208961/101‑1 (MQ=255)
                                 ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCgcgg                                                                      >  2:5860343/1‑101 (MQ=255)
                                  cGGGATNNGGGAGGNANNNGGAN...GGGN..NNNNGTCATCTTGCCGAAAAGAGCCCGGGGGGGGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCgcggg                                                                     <  1:4935326/101‑1 (MQ=16)
                                  cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.C.CCCCtgccc                                                                        >  2:7123796/1‑97 (MQ=0)
                                  cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.C.CCCCtgccc                                                                        >  1:2695792/1‑97 (MQ=0)
                                  cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCCAAAGGCCA.AGAGGGGGCAGCG.C.CCCCTTccc                                                                        <  1:6832091/101‑3 (MQ=0)
                                   gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCgcgggc                                                                    >  2:1144534/1‑101 (MQ=255)
                                   gggATATTTGAGCAAAGTTGAA...GGGG..GGCTTTAATCTTGCCGAAAAAATCCCGGGGGGGGAA.TGGGGCA...GCCAGAG.GGGGGCGGCGCC.CCCCTTCgcgggc                                                                    >  2:3741255/1‑101 (MQ=255)
                                        atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                               >  1:1625825/1‑101 (MQ=255)
                                         tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                              >  2:221725/1‑101 (MQ=255)
                                            gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAc                                                           <  1:5047355/101‑1 (MQ=255)
                                             aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcg                                                          >  2:5770225/1‑101 (MQ=255)
                                                  aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACgcgcgc                                                     <  1:4762198/101‑1 (MQ=255)
                                                   gaagAA...GGGG..GACTTTCATCTTGGCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgcgcgac                                                    <  2:5350376/98‑3 (MQ=25)
                                                   gATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                    <  2:4390570/101‑1 (MQ=255)
                                                   gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGGGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                    >  1:715783/1‑101 (MQ=255)
                                                    aTGAA...GGGG..TGCGTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                   <  2:1376315/101‑1 (MQ=25)
                                                    aTGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGagccg                                                   >  2:840316/1‑101 (MQ=37)
                                                     tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                  <  1:2206746/101‑1 (MQ=255)
                                                      gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                 >  1:2396561/1‑101 (MQ=255)
                                                       aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.CACCCCTTCGCGGGCGGTCGCCACGGGCGCCGCaa                                                >  2:4028710/1‑101 (MQ=25)
                                                            gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                              <  1:3782101/101‑1 (MQ=255)
                                                            gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                              >  1:4009044/1‑101 (MQ=255)
                                                                  ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                          >  2:5093846/1‑101 (MQ=255)
                                                                  ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                          <  2:2100716/101‑1 (MQ=255)
                                                                     tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                       <  1:2949020/101‑1 (MQ=255)
                                                                     tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                       >  1:6683171/1‑101 (MQ=255)
                                                                      ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                      >  1:5823355/1‑101 (MQ=255)
                                                                      ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                      >  2:1522249/1‑101 (MQ=255)
                                                                       tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGGGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGACGTGcc                                     >  2:6090815/1‑101 (MQ=37)
                                                                       tCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCACCAGGTCGTGcc                                     >  1:773936/1‑101 (MQ=37)
                                                                        cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                    >  1:3615629/1‑101 (MQ=255)
                                                                        cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                    >  1:6766337/1‑101 (MQ=255)
                                                                        cATCGTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                    <  2:2633081/101‑1 (MQ=255)
                                                                         aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                   <  1:6118507/101‑1 (MQ=255)
                                                                          tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                                  >  2:650504/1‑101 (MQ=255)
                                                                           cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                 >  1:429545/1‑101 (MQ=255)
                                                                           cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                 <  2:4807526/101‑1 (MQ=255)
                                                                            ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTGGCGGGCGGCCGCGACGCGCGCCGCAACAGTTGGTGCCCCttt                                >  1:5565268/1‑101 (MQ=25)
                                                                              gcagAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                              <  1:4480838/98‑1 (MQ=255)
                                                                                cGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                            <  2:6273172/101‑1 (MQ=255)
                                                                                 gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                           >  1:1861232/1‑101 (MQ=255)
                                                                                 gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                           >  2:1590847/1‑101 (MQ=255)
                                                                                  aaaaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                          >  1:1091367/1‑101 (MQ=255)
                                                                                   aaaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                         >  1:3486627/1‑101 (MQ=255)
                                                                                   aaaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                         <  1:1249388/101‑1 (MQ=255)
                                                                                    aaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                        >  2:4712624/1‑101 (MQ=255)
                                                                                     atatCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                       >  1:1361896/1‑101 (MQ=255)
                                                                                     atatCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                       >  1:1550525/1‑101 (MQ=255)
                                                                                     atatCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                       >  2:1069444/1‑101 (MQ=255)
                                                                                       atCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGTGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                     <  1:3406850/101‑1 (MQ=255)
                                                                                       atCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                     >  2:325359/1‑101 (MQ=255)
                                                                                         cccGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                   <  1:6168075/101‑1 (MQ=255)
                                                                                           cGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                 >  1:5649607/1‑101 (MQ=255)
                                                                                           cGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                 >  2:3499794/1‑101 (MQ=255)
                                                                                           cGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                 <  1:3840380/101‑1 (MQ=255)
                                                                                            ggggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                >  2:2454631/1‑101 (MQ=255)
                                                                                            ggggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                >  1:6042181/1‑101 (MQ=255)
                                                                                            ggggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                >  2:1860484/1‑101 (MQ=255)
                                                                                             gggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc               <  1:1947744/101‑1 (MQ=255)
                                                                                              ggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa              <  1:464075/101‑1 (MQ=255)
                                                                                               gggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt             >  2:5639484/1‑101 (MQ=255)
                                                                                               gggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt             <  2:2349527/101‑1 (MQ=255)
                                                                                               gggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt             <  1:4387506/101‑1 (MQ=255)
                                                                                                ggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg            >  2:776699/1‑101 (MQ=255)
                                                                                                  tGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc          <  1:2925490/101‑1 (MQ=255)
                                                                                                  tGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc          >  1:3206156/1‑101 (MQ=255)
                                                                                                  tGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGGCGCACCCATGTc          <  1:5126367/101‑1 (MQ=255)
                                                                                                  tGAA.TGCCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc          <  1:2804260/101‑1 (MQ=255)
                                                                                                   gAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa         >  2:6197319/1‑101 (MQ=255)
                                                                                                   gAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa         >  2:5974579/1‑101 (MQ=255)
                                                                                                    aa.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt        <  1:17518/101‑1 (MQ=255)
                                                                                                    aa.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt        <  1:2149551/101‑1 (MQ=255)
                                                                                                    aa.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTGCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt        <  2:3932233/101‑1 (MQ=255)
                                                                                                       tGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa      <  2:1990331/101‑1 (MQ=255)
                                                                                                        ggCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg     >  2:5485932/1‑101 (MQ=255)
                                                                                                           |                                                                                                         
GGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTG  >  NC_014034/2249273‑2249471

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG