Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,373 C→A 3.4% intergenic (‑169/‑136) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3730C→A 3.4% 21.7 ‑0.5 ‑0.7383intergenic (‑169/‑136)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (8/5):  ref base (193/177):  total (201/182)
Fisher's exact test strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.79e-01

Only 100 of 409 total aligned reads displayed.

GGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGG  >  NC_014034/2249274‑2249472
                                                                                                       |                                                                                                        
ggCTCTGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGTGGTGAA.TGgcgc                                                                                                          <  1:5131447/101‑1 (MQ=255)
  ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...g                                                                                                     >  2:1947672/1‑101 (MQ=255)
  ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...g                                                                                                     <  2:1547515/101‑1 (MQ=255)
  ctctGCCCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...g                                                                                                     >  2:440226/1‑101 (MQ=255)
    ctgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...Gcc                                                                                                   >  1:1474279/5‑101 (MQ=255)
     tgcccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCa                                                                                                  <  1:2984073/98‑1 (MQ=255)
       cccccGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCaga                                                                                                <  2:3161145/100‑1 (MQ=255)
         cccgtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAgg                                                                                              >  2:3838024/1‑101 (MQ=255)
            gtcccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAggggg                                                                                           <  1:3627731/101‑1 (MQ=255)
              cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGc                                                                                         <  1:2169459/101‑1 (MQ=255)
              cccgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGc                                                                                         >  1:2832057/1‑101 (MQ=255)
                cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAg                                                                                       <  1:1649347/101‑1 (MQ=255)
                cgtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAg                                                                                       <  2:6706884/101‑1 (MQ=255)
                 gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAgc                                                                                      >  1:2098610/1‑101 (MQ=255)
                 gtGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAgc                                                                                      >  2:1023740/1‑101 (MQ=255)
                        gACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCTAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.c.ccccc                                                                             <  2:5441827/101‑2 (MQ=255)
                         aCTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.c.ccccct                                                                            <  2:68211/101‑3 (MQ=255)
                          cgcccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.c.ccccctt                                                                           <  2:4144243/99‑4 (MQ=255)
                          cTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TTGCGCA...GCCAGAGGGGGGCAGCG.c.ccccctt                                                                           <  2:1314294/101‑4 (MQ=255)
                          cTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.c.ccccctt                                                                           <  2:6863426/101‑4 (MQ=255)
                           tCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.c.cccccttc                                                                          <  2:1394327/101‑5 (MQ=255)
                           tCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.c.cccccttc                                                                          >  1:6699743/1‑97 (MQ=255)
                            cccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.c.cccccttcg                                                                         <  2:2863072/101‑6 (MQ=255)
                               ccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCCAAAGGCCAAGAGGGGGCAGCG.C.CCCCtgcc                                                                          <  1:3095373/101‑4 (MQ=0)
                               ccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgg                                                                       >  2:5860343/1‑101 (MQ=255)
                                cGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGAGGGGGCAGCG.C.CCCCtgccc                                                                         >  1:1102536/1‑97 (MQ=0)
                                cGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGAGGGGGCAGCG.C.CCCCtgccc                                                                         >  2:7123796/1‑97 (MQ=0)
                                 gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggc                                                                     <  1:6769494/101‑1 (MQ=255)
                                 gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggc                                                                     >  2:1144534/1‑101 (MQ=255)
                                 gggATATTTGAGCAAAGTTGAAGGGG..GGCTTTAATCTTGCCGAAAAAATCCCGGGGGGGGAA.TGGGGCA...GCCAGAGGGGGGCGGCGCC.CCCCTTCgcgggc                                                                     >  2:3741255/1‑101 (MQ=255)
                                   gATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggcgg                                                                   <  1:4112880/101‑1 (MQ=255)
                                    atatTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGGA.TGGCGCA...GCCAGAGGGGGGCAGGGCC.CCCCTTCGCCggcggc                                                                  >  1:6513119/1‑101 (MQ=38)
                                      atTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                                >  1:3734564/1‑101 (MQ=255)
                                      atTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                                <  1:2418644/101‑1 (MQ=255)
                                      atTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                                >  1:481809/1‑101 (MQ=255)
                                      atTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                                >  1:533331/1‑101 (MQ=255)
                                           aGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcg                                                           >  2:5770225/1‑101 (MQ=255)
                                            ggCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgc                                                          <  1:2935905/101‑1 (MQ=255)
                                            ggCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgc                                                          <  1:1378941/101‑1 (MQ=255)
                                              cAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgcgc                                                        >  2:4362411/1‑101 (MQ=255)
                                                 gaagAAGGGG..GACTTTCATCTTGGCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgcgcgac                                                     <  2:5350376/98‑3 (MQ=25)
                                                 gATGGAGGGG..GGCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                     <  1:1593521/101‑1 (MQ=37)
                                                 gATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGGGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                     >  1:715783/1‑101 (MQ=255)
                                                  aTGAAGGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGagccg                                                    >  2:840316/1‑101 (MQ=37)
                                                    gAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                                                  <  2:2063484/101‑1 (MQ=255)
                                                     aaGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.CACCCCTTCGCGGGCGGTCGCCACGGGCGCCGCaa                                                 >  2:4028710/1‑101 (MQ=25)
                                                         gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                             >  2:3082247/1‑101 (MQ=255)
                                                          g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                            >  1:3438974/1‑101 (MQ=255)
                                                             gtcTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                           <  2:1663187/99‑1 (MQ=255)
                                                             ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                           >  2:5093846/1‑101 (MQ=255)
                                                              gCTTTCATCTTGACGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                                          >  1:5080654/1‑101 (MQ=255)
                                                                tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                        <  2:2770006/101‑1 (MQ=255)
                                                                 ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                       >  1:5823355/1‑101 (MQ=255)
                                                                  tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGGGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGACGTGcc                                      >  2:6090815/1‑101 (MQ=37)
                                                                  tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                      >  2:2562612/1‑101 (MQ=255)
                                                                  tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                      >  2:2436999/1‑101 (MQ=255)
                                                                  tCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCACCAGGTCGTGcc                                      >  1:773936/1‑101 (MQ=37)
                                                                   cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                     >  1:395411/1‑101 (MQ=255)
                                                                    aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                    <  1:6118507/101‑1 (MQ=255)
                                                                    aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                    <  1:1185207/101‑1 (MQ=255)
                                                                     tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCt                                   >  2:650504/1‑101 (MQ=255)
                                                                      cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                  <  2:4807526/101‑1 (MQ=255)
                                                                      cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGCCCCtt                                  >  1:3269017/1‑101 (MQ=255)
                                                                       ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                 <  1:3351043/101‑1 (MQ=255)
                                                                       tggCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                 <  1:2718445/99‑1 (MQ=255)
                                                                         gcagAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                               <  1:4480838/98‑1 (MQ=255)
                                                                          ccaaaaaGATCCGGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                              <  2:5977560/98‑1 (MQ=38)
                                                                           cGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                             >  1:4308129/1‑101 (MQ=255)
                                                                           cGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                             <  2:6273172/101‑1 (MQ=255)
                                                                            gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                            >  1:1861232/1‑101 (MQ=255)
                                                                            gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                            <  2:300402/101‑1 (MQ=255)
                                                                               aaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                         >  1:3570184/1‑101 (MQ=255)
                                                                               aaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                         >  2:4712624/1‑101 (MQ=255)
                                                                                atatCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                        >  2:1069444/1‑101 (MQ=255)
                                                                                  atgccGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                      <  2:2309958/98‑1 (MQ=255)
                                                                                  atCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                      >  2:325359/1‑101 (MQ=255)
                                                                                   tCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCg                     <  1:6946437/101‑1 (MQ=255)
                                                                                    cccGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                    <  1:6168075/101‑1 (MQ=255)
                                                                                      cGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                  >  2:3499794/1‑101 (MQ=255)
                                                                                      cGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                  >  1:5649607/1‑101 (MQ=255)
                                                                                       ggggggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                 >  1:6042181/1‑101 (MQ=255)
                                                                                       ggggggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                 >  2:1860484/1‑101 (MQ=255)
                                                                                        gggggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                >  1:1764263/1‑101 (MQ=255)
                                                                                        gggggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                <  1:1947744/101‑1 (MQ=255)
                                                                                         ggggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa               <  2:2193081/101‑1 (MQ=255)
                                                                                         ggggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa               <  1:464075/101‑1 (MQ=255)
                                                                                          gggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt              >  2:5639484/1‑101 (MQ=255)
                                                                                           ggTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg             >  2:776699/1‑101 (MQ=255)
                                                                                            gTGAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt            <  1:1319463/101‑1 (MQ=255)
                                                                                              gAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          >  1:1085864/1‑101 (MQ=255)
                                                                                              gAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          >  1:2250550/1‑101 (MQ=255)
                                                                                              gAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          >  2:6197319/1‑101 (MQ=255)
                                                                                              gAA.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa          <  1:1023775/101‑1 (MQ=255)
                                                                                               aa.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt         >  1:6669815/1‑101 (MQ=255)
                                                                                               aa.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt         <  1:3821049/101‑1 (MQ=255)
                                                                                               aa.TGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTGCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt         <  2:3932233/101‑1 (MQ=255)
                                                                                                  tGGCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa       <  1:4943778/101‑1 (MQ=255)
                                                                                                   ggCGCA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg      >  2:5485932/1‑101 (MQ=255)
                                                                                                     cgcA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt    >  1:5590687/1‑101 (MQ=255)
                                                                                                       cA...GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg  >  1:4393090/1‑101 (MQ=255)
                                                                                                       |                                                                                                        
GGCTCTG.CCCCGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGG  >  NC_014034/2249274‑2249472

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG