Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,379 G→A 1.8% intergenic (‑175/‑130) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3790G→A 1.8% 7.5 ‑0.8 ‑0.0380intergenic (‑175/‑130)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (195/177):  total (200/180)
Fisher's exact test strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.62e-01

Only 100 of 409 total aligned reads displayed.

TGCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAT  >  NC_014034/2249279‑2249478
                                                                                                              |                                                                                                      
gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCag                                                                                                        >  2:5410155/3‑101 (MQ=255)
 cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCaga                                                                                                       <  2:3161145/100‑1 (MQ=255)
       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAg.ggggg                                                                                                <  2:4114806/101‑1 (MQ=255)
          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAg                                                                                             <  1:1649347/101‑1 (MQ=255)
           gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgc                                                                                            >  1:2098610/1‑101 (MQ=255)
            caagcggACTCCCCCCGGGATATTTGGAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA...GCCAGAg.ggggga                                                                                               >  1:4752660/6‑100 (MQ=18)
            caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA...GCCAGAG.GGGGGc                                                                                               <  2:6828971/96‑1 (MQ=18)
             gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAgcg.c                                                                                         >  1:346240/1‑101 (MQ=255)
              ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.c                                                                                       <  2:1744695/101‑1 (MQ=255)
               cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cc                                                                                      >  2:2166404/1‑101 (MQ=255)
                gggGCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccc                                                                                     <  2:2636775/101‑1 (MQ=255)
                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccc                                                                                     <  1:6323577/101‑1 (MQ=255)
                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccc                                                                                     >  2:1144738/1‑101 (MQ=255)
                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccc                                                                                    >  1:3638035/1‑101 (MQ=255)
                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccc                                                                                    >  2:6246519/1‑101 (MQ=255)
                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccc                                                                                    >  1:3079255/1‑101 (MQ=255)
                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.ccccctt                                                                                 <  1:741631/101‑4 (MQ=255)
                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccccttc                                                                                <  2:1394327/101‑5 (MQ=255)
                     t.CCCCCGGGATATCTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.c.cccccttc                                                                                <  2:4337424/101‑5 (MQ=255)
                           cGGGATNNGGGAGGNANNNGGAN...GGGN..NNNNGTCATCTTGCCGAAAAGAGCCCGGGGGGGGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCgcggg                                                                            <  1:4935326/101‑1 (MQ=16)
                           cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.C.CCCCtgccc                                                                               >  2:2012580/1‑97 (MQ=0)
                           cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.C.CCCCtgccc                                                                               >  1:1102536/1‑97 (MQ=0)
                           cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAG.GCCAAGA.GGGGGCAGCG.C.CCCCtgccc                                                                               >  2:7123796/1‑97 (MQ=0)
                           cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAA.TTGGCCAAAGGCCA.AGAGGGGGCAGCG.C.CCCCTTccc                                                                               <  1:6832091/101‑3 (MQ=0)
                           cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCgcggg                                                                            >  2:4408840/1‑101 (MQ=255)
                            gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TAGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCgcgggc                                                                           >  2:2394347/1‑101 (MQ=255)
                             ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCCGCGCC.CCCCTTCgcgggcg                                                                          >  2:3008515/1‑101 (MQ=255)
                                tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGcc                                                                       >  2:5920788/1‑101 (MQ=255)
                                 atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCcg                                                                      >  1:533331/1‑101 (MQ=255)
                                  tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCTCGGGGGGGGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                                     >  1:7068660/1‑101 (MQ=37)
                                   ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgcg                                                                    >  2:1572422/1‑101 (MQ=255)
                                          aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgcgcg                                                             >  2:3921484/1‑101 (MQ=255)
                                           aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACgcgcgc                                                            >  1:664077/1‑101 (MQ=255)
                                            gATGGA...GGGG..GGCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                           <  1:1593521/101‑1 (MQ=37)
                                            gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                           <  2:552067/101‑1 (MQ=255)
                                            gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                           >  2:6157799/1‑101 (MQ=255)
                                             aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGTAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                          <  2:6910577/101‑1 (MQ=255)
                                             aTGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGagccg                                                          >  2:840316/1‑101 (MQ=37)
                                              tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                         <  1:2206746/101‑1 (MQ=255)
                                                 a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                      >  2:2828444/1‑101 (MQ=255)
                                                 a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                      >  2:6629257/1‑101 (MQ=255)
                                                           ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                 >  2:5093846/1‑101 (MQ=255)
                                                             cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                                               >  1:4157106/1‑101 (MQ=255)
                                                              tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                              >  1:3613464/1‑101 (MQ=255)
                                                              tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                              <  1:2949020/101‑1 (MQ=255)
                                                              tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                              >  1:6683171/1‑101 (MQ=255)
                                                               ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                             >  1:3966207/1‑101 (MQ=255)
                                                               ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                             >  1:5823355/1‑101 (MQ=255)
                                                                 cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                           >  1:6766337/1‑101 (MQ=255)
                                                                 cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.CGGGGCAGCG.CACCCCTTCGCGGGCGGCCGCGGAGCGCGCCGCAACTGGTCgaccac                                           >  2:579232/1‑96 (MQ=25)
                                                                  aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                          <  1:1185207/101‑1 (MQ=255)
                                                                  aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                          <  1:6118507/101‑1 (MQ=255)
                                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                        <  2:4807526/101‑1 (MQ=255)
                                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                        >  1:2357460/1‑101 (MQ=255)
                                                                    cTTACCGAAGTTATGCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                        <  1:2783596/101‑1 (MQ=37)
                                                                     ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                       <  1:3351043/101‑1 (MQ=255)
                                                                        ccGAAAATATCCGGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                    <  2:3814525/101‑1 (MQ=255)
                                                                        ccGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                    <  2:289923/101‑1 (MQ=255)
                                                                        ccGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                    <  2:402648/101‑1 (MQ=255)
                                                                        ccGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                    <  2:3719750/101‑1 (MQ=255)
                                                                         cGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                   <  2:1316993/101‑1 (MQ=255)
                                                                          gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                  <  1:3838187/101‑1 (MQ=255)
                                                                          gAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                  >  1:1861232/1‑101 (MQ=255)
                                                                           aaaaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                 <  2:6022966/101‑1 (MQ=255)
                                                                            aaaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                <  1:1249388/101‑1 (MQ=255)
                                                                            aaaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                >  1:3764805/1‑101 (MQ=255)
                                                                             aaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                               <  2:1003407/101‑1 (MQ=255)
                                                                             aaTATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                               >  2:4712624/1‑101 (MQ=255)
                                                                              atatCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                              >  1:1361896/1‑101 (MQ=255)
                                                                              atatCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                              >  2:1069444/1‑101 (MQ=255)
                                                                                atCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                            >  2:325359/1‑101 (MQ=255)
                                                                                  cccGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                          <  1:6168075/101‑1 (MQ=255)
                                                                                    cGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                        >  2:3499794/1‑101 (MQ=255)
                                                                                    cGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                        >  1:5649607/1‑101 (MQ=255)
                                                                                     ggggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                       >  2:6345672/1‑101 (MQ=255)
                                                                                     ggggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                       >  1:6042181/1‑101 (MQ=255)
                                                                                      gggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGCCGCGCGCCGCAACAGGGCGTGCCCCTTTGTCATTCTGCCGCAccc                      >  2:2297761/1‑101 (MQ=38)
                                                                                      gggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                      >  1:1764263/1‑101 (MQ=255)
                                                                                      gggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                      <  1:1947744/101‑1 (MQ=255)
                                                                                       ggggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                     >  1:2690359/1‑101 (MQ=255)
                                                                                        gggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                    <  1:4387506/101‑1 (MQ=255)
                                                                                         ggTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                   >  2:776699/1‑101 (MQ=255)
                                                                                           tGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                 >  1:3206156/1‑101 (MQ=255)
                                                                                           tGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                 <  1:2925490/101‑1 (MQ=255)
                                                                                           tGAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGGCGCACCCATGTc                 <  1:5126367/101‑1 (MQ=255)
                                                                                            gAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                <  1:1023775/101‑1 (MQ=255)
                                                                                            gAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                >  1:1085864/1‑101 (MQ=255)
                                                                                            gAA.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                >  2:249310/1‑101 (MQ=255)
                                                                                             aa.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt               >  1:4467815/1‑101 (MQ=255)
                                                                                              a.TGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa              >  2:6724162/1‑101 (MQ=255)
                                                                                                tGGCGCA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa             >  2:5639592/1‑101 (MQ=255)
                                                                                                    gcA...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg         >  1:4576935/1‑101 (MQ=255)
                                                                                                      a...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc       <  2:3094865/101‑1 (MQ=255)
                                                                                                      a...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc       >  2:1954902/1‑101 (MQ=255)
                                                                                                      a...GCCAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc       >  2:1888541/1‑101 (MQ=255)
                                                                                                           ccAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCNTTCTGTCGCACCCATGTCATAAGCTGGCCg     >  1:1467791/1‑101 (MQ=255)
                                                                                                            cAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc    >  2:5443691/1‑101 (MQ=255)
                                                                                                            cAGAG.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGACATTCTGTCGCACCCATGTCATAAGCTGGCCGc    <  1:5024730/101‑1 (MQ=255)
                                                                                                              gag.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt  <  1:2166484/101‑1 (MQ=255)
                                                                                                              gag.GGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt  <  1:5573573/101‑1 (MQ=255)
                                                                                                              |                                                                                                      
TGCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA...GCCAGAG.GGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAT  >  NC_014034/2249279‑2249478

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG