Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,380 A→G 1.8% intergenic (‑176/‑129) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3800A→G 1.8% 5.5 ‑0.8 ‑0.7380intergenic (‑176/‑129)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (194/178):  total (200/180)
Fisher's exact test strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.23e-01

Only 100 of 407 total aligned reads displayed.

CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.C.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATC  >  NC_014034/2249281‑2249479
                                                                                                            |                                                                                                      
  ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                                     <  1:1987405/101‑1 (MQ=255)
    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                                                   <  1:3627731/101‑1 (MQ=255)
    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                                                   >  2:3222722/1‑101 (MQ=255)
     tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                                  <  2:4114806/101‑1 (MQ=255)
      cgcgtGCCGAGATT.GCCCCGGGATATTTGAGTGAAGATGAC...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                 <  2:1373054/99‑1 (MQ=25)
      cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                                                 >  2:5818469/1‑101 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                               >  1:603282/1‑101 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                               <  1:1649347/101‑1 (MQ=255)
          caagcggACTCCCCCCGGGATATTTGGAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggga                                                                                                 >  1:4752660/6‑100 (MQ=18)
          caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                                 <  2:6828971/96‑1 (MQ=18)
           tccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                                                                                           <  2:1031953/100‑1 (MQ=255)
              gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccc                                                                                       <  1:6323577/101‑1 (MQ=255)
               ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccc                                                                                      >  2:6246519/1‑101 (MQ=255)
               ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccc                                                                                      >  2:1249176/1‑101 (MQ=255)
               ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccc                                                                                      >  1:3079255/1‑101 (MQ=255)
                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccct                                                                                    <  1:5280347/101‑3 (MQ=255)
                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccctt                                                                                   <  1:2198269/101‑4 (MQ=255)
                   t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccccttc                                                                                  <  1:6739474/101‑5 (MQ=255)
                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccccttcg                                                                                 <  2:2863072/101‑6 (MQ=255)
                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccccttcg                                                                                 >  2:3151444/1‑96 (MQ=255)
                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.C.CCCC.tgccc                                                                                >  1:1102536/1‑97 (MQ=0)
                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.C.CCCC.tgccc                                                                                >  2:7123796/1‑97 (MQ=0)
                          gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCgcgggc                                                                            >  2:1144534/1‑101 (MQ=255)
                           ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCgcgggcg                                                                           >  2:191590/1‑101 (MQ=255)
                             atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGggcggc                                                                         >  1:6078253/1‑101 (MQ=255)
                               atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCcg                                                                       >  1:3734564/1‑101 (MQ=255)
                                    aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGAcg                                                                  >  2:1500299/1‑101 (MQ=255)
                                      gCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGAcgcg                                                                >  1:5636751/1‑101 (MQ=255)
                                        aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGAcgcgcg                                                              >  2:3921484/1‑101 (MQ=255)
                                         aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACgcgcgc                                                             >  1:6603896/1‑101 (MQ=255)
                                          gaagAA...GGGG..GACTTTCATCTTGGCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGAcgcgcgac                                                            <  2:5350376/98‑3 (MQ=25)
                                          gATGGA...GGGG..GGCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGcgcc                                                            <  1:1593521/101‑1 (MQ=37)
                                          gATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGcgcc                                                            <  2:4390570/101‑1 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGcgcc                                                            <  1:6791908/101‑1 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGcgcc                                                            >  2:6157799/1‑101 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGcgcc                                                            <  2:552067/101‑1 (MQ=255)
                                           aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGTAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGcgccg                                                           <  2:6910577/101‑1 (MQ=255)
                                           aTGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGagccg                                                           >  2:840316/1‑101 (MQ=37)
                                             gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCa                                                         <  2:2063484/101‑1 (MQ=255)
                                              aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.CTCGCGGGCGGCCGCGCCGCGGGCCGCaa                                                        >  2:4330687/1‑101 (MQ=25)
                                               a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                       >  2:6629257/1‑101 (MQ=255)
                                                     gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                                    >  2:3082247/1‑101 (MQ=255)
                                                     gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.C.CCCCATTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                                    <  1:5007893/101‑1 (MQ=255)
                                                      g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                                   >  1:3438974/1‑101 (MQ=255)
                                                      g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                                   <  2:5049094/101‑1 (MQ=255)
                                                           cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                                                >  1:4157106/1‑101 (MQ=255)
                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                               >  2:5654839/1‑101 (MQ=255)
                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                               <  2:2770006/101‑1 (MQ=255)
                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                               <  1:2949020/101‑1 (MQ=255)
                                                              tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                             >  2:2436999/1‑101 (MQ=255)
                                                              tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                             <  2:5934343/101‑1 (MQ=255)
                                                              tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                             >  2:2562612/1‑101 (MQ=255)
                                                               cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                            >  1:720349/1‑101 (MQ=255)
                                                               cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                            >  1:395411/1‑101 (MQ=255)
                                                                aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                           <  1:1185207/101‑1 (MQ=255)
                                                                  cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                         >  1:2357460/1‑101 (MQ=255)
                                                                  cTTACCGAAGTTATGCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                         <  1:2783596/101‑1 (MQ=37)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTGGCGGGCGGCCGCGACGCGCGCCGCAACAGTTGGTGCCCCttt                                        >  1:5565268/1‑101 (MQ=25)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                        <  1:3351043/101‑1 (MQ=255)
                                                                      ccGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                     <  2:3814525/101‑1 (MQ=255)
                                                                      ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                     <  2:402648/101‑1 (MQ=255)
                                                                      ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                     <  2:3719750/101‑1 (MQ=255)
                                                                      ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                     <  1:859924/101‑1 (MQ=255)
                                                                        gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                   >  1:1861232/1‑101 (MQ=255)
                                                                        gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                   >  2:1590847/1‑101 (MQ=255)
                                                                        gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                   <  2:300402/101‑1 (MQ=255)
                                                                         aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                  <  2:6022966/101‑1 (MQ=255)
                                                                          aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                 <  1:1249388/101‑1 (MQ=255)
                                                                           aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                >  1:3570184/1‑101 (MQ=255)
                                                                            atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                               >  1:1361896/1‑101 (MQ=255)
                                                                              atgccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                             <  2:2309958/98‑1 (MQ=255)
                                                                                cccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                           <  1:6168075/101‑1 (MQ=255)
                                                                                  cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                         >  2:3499794/1‑101 (MQ=255)
                                                                                   ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                        >  2:6345672/1‑101 (MQ=255)
                                                                                   ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                        >  1:6682385/1‑101 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                       <  1:5818771/101‑1 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                       <  1:4870052/101‑1 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                       >  1:1764263/1‑101 (MQ=255)
                                                                                     ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                      >  1:2690359/1‑101 (MQ=255)
                                                                                     ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                      <  2:2193081/101‑1 (MQ=255)
                                                                                      gggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                     <  1:4387506/101‑1 (MQ=255)
                                                                                       ggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                    >  2:776699/1‑101 (MQ=255)
                                                                                         tGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                  >  1:3206156/1‑101 (MQ=255)
                                                                                         tGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                  <  1:2925490/101‑1 (MQ=255)
                                                                                          gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                 >  1:1085864/1‑101 (MQ=255)
                                                                                          gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                 <  1:1023775/101‑1 (MQ=255)
                                                                                           aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGGCGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                >  2:4625112/1‑101 (MQ=255)
                                                                                           aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                <  1:2149551/101‑1 (MQ=255)
                                                                                           aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                <  1:3821049/101‑1 (MQ=255)
                                                                                           aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                >  1:4467815/1‑101 (MQ=255)
                                                                                            a.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa               >  2:479309/1‑101 (MQ=255)
                                                                                            a.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa               >  2:6724162/1‑101 (MQ=255)
                                                                                            a.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa               <  1:5112721/101‑1 (MQ=255)
                                                                                              tGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa              <  1:6966959/101‑1 (MQ=255)
                                                                                                  gcA..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg          >  1:4576935/1‑101 (MQ=255)
                                                                                                    a..GCCAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc        <  2:1980247/101‑1 (MQ=255)
                                                                                                       gctagagGGGGGCAGCG.CGCCCC.TACGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGcc       <  2:1790403/98‑1 (MQ=255)
                                                                                                        ccAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCNTTCTGTCGCACCCATGTCATAAGCTGGCCg      >  1:1467791/1‑101 (MQ=255)
                                                                                                         cAGAGGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc     >  2:5443691/1‑101 (MQ=255)
                                                                                                            agGGGGGCAGCGCC.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc  <  1:2092121/101‑1 (MQ=255)
                                                                                                            |                                                                                                      
CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.C.CCCC.TTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATC  >  NC_014034/2249281‑2249479

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG