Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,381 G→A 1.9% intergenic (‑177/‑128) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3810G→A 1.9% 7.6 ‑0.8 ‑0.0364intergenic (‑177/‑128)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (187/170):  total (192/172)
Fisher's exact test strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.30e-01

Only 100 of 409 total aligned reads displayed.

CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCG  >  NC_014034/2249281‑2249480
                                                                                                             |                                                                                                     
ccccCTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                                                       <  2:2304837/101‑1 (MQ=255)
  ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                                     <  1:1987405/101‑1 (MQ=255)
    gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                                                   >  2:3222722/1‑101 (MQ=255)
     tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                                                  >  1:6248853/1‑101 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCGTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                               >  1:3616988/1‑101 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                               >  2:1339030/1‑101 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                               <  2:3073668/101‑1 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                               >  1:603282/1‑101 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                               <  1:1649347/101‑1 (MQ=255)
        cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.AGGCGCA..GCCAGAGGGGGGCAg                                                                                               >  1:497490/1‑101 (MQ=255)
         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                              <  1:4434498/101‑1 (MQ=255)
         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                              >  1:2098610/1‑101 (MQ=255)
         gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                                                                              >  2:1023740/1‑101 (MQ=255)
          caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                                                 <  2:6828971/96‑1 (MQ=18)
             gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgc                                                                                              <  1:3722323/99‑1 (MQ=18)
               ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccc                                                                                      >  2:6246519/1‑101 (MQ=255)
               ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccc                                                                                      <  2:1092191/101‑1 (MQ=255)
                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTTATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccct                                                                                    <  1:6672530/101‑3 (MQ=255)
                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccct                                                                                    <  1:5280347/101‑3 (MQ=255)
                 aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccct                                                                                    >  1:3128543/1‑99 (MQ=255)
                  cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.ccccctt                                                                                   <  2:1236503/101‑4 (MQ=255)
                     cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccccttcg                                                                                 >  1:4684026/1‑96 (MQ=255)
                     cccccGGAATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAGCTGGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.c.cccccttcg                                                                                 <  1:6161009/101‑6 (MQ=25)
                        ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgg                                                                               >  2:2854614/1‑101 (MQ=255)
                        ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgg                                                                               <  1:3788549/101‑1 (MQ=255)
                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.C.CCCCtgccc                                                                                 >  1:1102536/1‑97 (MQ=0)
                         cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.C.CCCCtgccc                                                                                 >  2:7123796/1‑97 (MQ=0)
                          gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCgcgggc                                                                             <  1:355356/101‑1 (MQ=255)
                             atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGggcggc                                                                          >  1:6078253/1‑101 (MQ=255)
                             atatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGGA.TGGCGCA..GCCAGAGGGGGGCAGGGCC.CCCCTTCGCCggcggc                                                                          >  1:6513119/1‑101 (MQ=38)
                              tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGcc                                                                         >  2:5920788/1‑101 (MQ=255)
                                tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                                       <  1:3300863/101‑1 (MQ=255)
                                tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCcgc                                                                       <  2:3958137/101‑1 (MQ=255)
                                    aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcg                                                                   >  2:1500299/1‑101 (MQ=255)
                                    aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcg                                                                   >  2:1909981/1‑101 (MQ=255)
                                      gCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgcg                                                                 >  1:5636751/1‑101 (MQ=255)
                                          gaagAA...GGGG..GACTTTCATCTTGGCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGAcgcgcgac                                                             <  2:5350376/98‑3 (MQ=25)
                                          gATGGA...GGGG..GGCTTTCATCTTGCCGAAAATTTGCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             <  1:1593521/101‑1 (MQ=37)
                                          gATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             <  2:4390570/101‑1 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGGGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             >  1:715783/1‑101 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             >  2:2428170/1‑101 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             <  2:552067/101‑1 (MQ=255)
                                          gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgcc                                                             >  2:6157799/1‑101 (MQ=255)
                                           aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGTAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccg                                                            <  2:6910577/101‑1 (MQ=255)
                                           aTGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGagccg                                                            >  2:840316/1‑101 (MQ=37)
                                            tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                                           <  1:2206746/101‑1 (MQ=255)
                                              aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCCTCGCGGGCGGCCGCGCCGCGGGCCGCaa                                                         >  2:4330687/1‑101 (MQ=25)
                                               a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                        >  2:6629257/1‑101 (MQ=255)
                                                    ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                      >  1:673427/1‑101 (MQ=255)
                                                      g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                                    <  2:5049094/101‑1 (MQ=255)
                                                      g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGCGCGGCCGCGACGCGCGCCGCAACAGGt                                                    <  2:2062465/101‑1 (MQ=255)
                                                          gCTTTCATCTTGACGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                                                  >  1:5080654/1‑101 (MQ=255)
                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCggg                                                >  1:3938455/1‑99 (MQ=255)
                                                            tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                <  1:2949020/101‑1 (MQ=255)
                                                              tCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcc                                              >  2:3498982/1‑101 (MQ=255)
                                                              tCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCACCAGGTCGTGcc                                              >  1:773936/1‑101 (MQ=37)
                                                               cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGCGGGGCAGCG.CACCCCTTCGCGGGCGGCCGCGGAGCGCGCCGCAACTGGTCgaccac                                             >  2:579232/1‑96 (MQ=25)
                                                                aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                            >  2:3887808/1‑101 (MQ=255)
                                                                aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                            <  1:1185207/101‑1 (MQ=255)
                                                                  cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                          >  1:2357460/1‑101 (MQ=255)
                                                                  cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                          >  1:4846329/1‑101 (MQ=255)
                                                                  cTTACCGAAGTTATGCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                          <  1:2783596/101‑1 (MQ=37)
                                                                   ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTGGCGGGCGGCCGCGACGCGCGCCGCAACAGTTGGTGCCCCttt                                         >  1:5565268/1‑101 (MQ=25)
                                                                    tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                        <  2:2533459/101‑1 (MQ=255)
                                                                     gcagAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                                       <  1:4480838/98‑1 (MQ=255)
                                                                       cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                     >  2:4054470/1‑101 (MQ=255)
                                                                       cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                     >  1:4308129/1‑101 (MQ=255)
                                                                       cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGCCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                     >  2:3119263/1‑101 (MQ=255)
                                                                        gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                    >  1:1861232/1‑101 (MQ=255)
                                                                        gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                    >  2:1590847/1‑101 (MQ=255)
                                                                         aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                   <  2:6022966/101‑1 (MQ=255)
                                                                          aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                  <  1:1249388/101‑1 (MQ=255)
                                                                            atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                                >  1:1361896/1‑101 (MQ=255)
                                                                              atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGTGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                              <  1:3406850/101‑1 (MQ=255)
                                                                               tCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCg                             <  1:6946437/101‑1 (MQ=255)
                                                                                cccGGGGGGTGGA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                            <  2:3713814/101‑1 (MQ=255)
                                                                                   ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                         >  2:6345672/1‑101 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                        >  1:1764263/1‑101 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                        <  1:5818771/101‑1 (MQ=255)
                                                                                    gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                        >  2:1762860/1‑101 (MQ=255)
                                                                                     ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                       >  1:2690359/1‑101 (MQ=255)
                                                                                       ggTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                     >  2:776699/1‑101 (MQ=255)
                                                                                        gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                    >  1:6776734/1‑101 (MQ=255)
                                                                                        gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                    <  1:4092467/101‑1 (MQ=255)
                                                                                        gTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                    <  2:2175832/101‑1 (MQ=255)
                                                                                         tGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                   <  1:2925490/101‑1 (MQ=255)
                                                                                          gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                  <  1:1023775/101‑1 (MQ=255)
                                                                                          gAA.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                  >  1:1085864/1‑101 (MQ=255)
                                                                                           aa.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGGCGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                 >  2:4625112/1‑101 (MQ=255)
                                                                                            a.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                >  2:6724162/1‑101 (MQ=255)
                                                                                            a.TGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                >  2:479309/1‑101 (MQ=255)
                                                                                              tGGCGCA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa               >  2:5639592/1‑101 (MQ=255)
                                                                                                  gcA..GCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg           >  2:3799744/1‑101 (MQ=255)
                                                                                                      ggCCAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc         <  2:1980246/100‑1 (MQ=255)
                                                                                                        ccAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCNTTCTGTCGCACCCATGTCATAAGCTGGCCg       >  1:1467791/1‑101 (MQ=255)
                                                                                                         cAGAGGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc      >  2:5443691/1‑101 (MQ=255)
                                                                                                          agagGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa     >  2:2989386/1‑101 (MQ=255)
                                                                                                           gagGGGGGCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt    <  1:2166484/101‑1 (MQ=255)
                                                                                                            gggggggCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc   <  1:30657/100‑1 (MQ=255)
                                                                                                             ggggggCAGCGCC.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCg  >  2:2717926/1‑101 (MQ=255)
                                                                                                             |                                                                                                     
CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.C.CCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCG  >  NC_014034/2249281‑2249480

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG