BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,254,818	A→G	7.0%	L16L (CTT→CTC)	RCAP_rcc02086 ←	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,254,818	0	A→G	7.0%	51.9 ‑2.7 ‑0.0	386	L16L (CTT→CTC)	RCAP_rcc02086	hypothetical protein
Reads supporting (aligned to +/- strand): new base (20/7): ref base (199/160): total (219/167)
Fisher's exact test strand distribution p-value = 7.04e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 411 total aligned reads displayed.


AACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCC  >  NC_014034/2254718‑2254918


aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                                                      >  2:4201775/1‑101 (MQ=255)


aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                                                      >  1:4241656/1‑101 (MQ=255)


  cTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAg                                                                                                    >  2:160195/1‑101 (MQ=255)


  cTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAg                                                                                                    <  1:4048726/101‑1 (MQ=255)


   tGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGa                                                                                                   <  1:1354006/101‑1 (MQ=255)


     gACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACg                                                                                                 >  2:4529855/1‑101 (MQ=255)


       cacCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGtt                                                                                               <  2:2963916/101‑1 (MQ=255)


         ccccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTc                                                                                             <  2:5345190/101‑1 (MQ=255)


          cccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCg                                                                                            <  2:6991623/101‑1 (MQ=255)


             aaaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccc                                                                                         >  2:4417798/1‑101 (MQ=255)


             aaaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccc                                                                                         >  2:5487824/1‑101 (MQ=255)


             aaaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccc                                                                                         <  2:5638030/101‑1 (MQ=255)


              aaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGcccc                                                                                        >  1:4108435/1‑101 (MQ=255)


               aCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccccc                                                                                       <  1:227050/101‑1 (MQ=255)


                cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAATGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                                                      >  2:5252015/1‑101 (MQ=255)


                cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                                                      >  1:2347680/1‑101 (MQ=255)


                cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGAGTCAAGGTCGAAGCGGTCGGAAAGACGTTTCGcccccg                                                                                      >  2:4752018/1‑100 (MQ=255)


                   gTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCt                                                                                   >  2:6544871/1‑101 (MQ=255)


                    tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCtt                                                                                  >  2:1525469/1‑101 (MQ=255)


                    tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCC.CCATGGGCAGCGCCGAGGCGAAGGGGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCttt                                                                                 >  1:5001779/1‑101 (MQ=255)


                     gATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCttt                                                                                 >  2:2094734/1‑101 (MQ=255)


                      aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                                                >  2:2436492/1‑101 (MQ=255)


                         gCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgc                                                                             >  2:3066794/1‑101 (MQ=255)


                         gCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgc                                                                             >  2:4948100/1‑101 (MQ=255)


                           aTGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcgc                                                                           >  1:3002731/1‑101 (MQ=255)


                           aTGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcgc                                                                           <  1:2913132/101‑1 (MQ=255)


                             gCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCgg                                                                         >  1:5818675/1‑101 (MQ=255)


                               cGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGa                                                                       >  2:3585412/1‑101 (MQ=255)


                                gTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAt                                                                      >  1:3559918/1‑101 (MQ=255)


                                gTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAt                                                                      <  2:6446186/101‑1 (MQ=255)


                                gTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAt                                                                      >  2:6169680/1‑101 (MQ=255)


                                 tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                                                     <  2:4642985/101‑1 (MQ=255)


                                 tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                                                     <  2:231009/101‑1 (MQ=255)


                                  ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                                                    >  2:4050102/1‑101 (MQ=255)


                                    ccccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTcc                                                                  >  1:4842329/1‑101 (MQ=255)


                                    ccccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTcc                                                                  <  1:2757809/101‑1 (MQ=255)


                                     cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                                                 <  1:6684116/101‑1 (MQ=255)


                                      ccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcag                                                                <  1:5196914/101‑1 (MQ=255)


                                      ccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcag                                                                >  1:5427235/1‑101 (MQ=255)


                                       cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                               >  1:4724342/9‑100 (MQ=255)


                                       cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                               >  2:1418534/9‑100 (MQ=255)


                                       cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                               <  2:3278421/93‑2 (MQ=255)


                                       cTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagc                                                               <  1:147788/101‑1 (MQ=255)


                                        ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCTAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                              <  2:5754968/101‑1 (MQ=255)


                                        gatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGGCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGCCGCGGGATGTCgaggc                                                              >  2:1240426/8‑99 (MQ=255)


                                         tCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagcag                                                             >  2:5939777/1‑101 (MQ=255)


                                          tcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccg                                                            <  2:1148570/96‑5 (MQ=255)


                                          cGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGt                                                            >  2:1000997/1‑101 (MQ=255)


                                           cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                                           >  2:6742983/5‑96 (MQ=255)


                                            ctctCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCg                                                          >  2:2175182/1‑101 (MQ=255)


                                             cgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgc                                                         <  2:7086579/99‑8 (MQ=255)


                                              ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCa                                                        >  1:6608656/1‑101 (MQ=255)


                                              ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCa                                                        <  1:4415619/101‑1 (MQ=255)


                                              ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGCCGCa                                                        >  2:2747481/1‑101 (MQ=255)


                                                cGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAg                                                      <  1:383012/101‑1 (MQ=255)


                                                 gCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAgg                                                     >  1:6243199/1‑101 (MQ=255)


                                                 gCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAgg                                                     <  1:1686309/101‑1 (MQ=255)


                                                  cccTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGt                                                    >  1:938267/1‑101 (MQ=255)


                                                        aTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTc                                              <  1:3764311/101‑1 (MQ=255)


                                                        aTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTc                                              >  1:5727685/1‑101 (MQ=255)


                                                            gCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCagag                                          <  1:686334/101‑1 (MQ=255)


                                                            gCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCagag                                          >  1:105281/1‑101 (MQ=255)


                                                               gcgcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACg                                       <  2:345075/101‑1 (MQ=255)


                                                                 gcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGcc                                     <  1:4530095/101‑1 (MQ=255)


                                                                  ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa                                    <  2:2582807/101‑1 (MQ=255)


                                                                  ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa                                    <  2:886119/101‑1 (MQ=255)


                                                                   cGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCaa                                   <  2:6080206/101‑1 (MQ=255)


                                                                    gAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAg                                  >  1:2553914/1‑101 (MQ=255)


                                                                      ggCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGt                                <  1:6086546/101‑1 (MQ=255)


                                                                         tccgatctCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                             <  2:3922278/94‑1 (MQ=255)


                                                                         gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                             <  2:785450/101‑1 (MQ=255)


                                                                         gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                             >  2:6003658/1‑101 (MQ=255)


                                                                         gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                             <  2:5117535/101‑1 (MQ=255)


                                                                          aaGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGGGCTTTCAGAGACGCCAAGGTAATg                            >  1:2376084/1‑101 (MQ=255)


                                                                           aGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATgc                           <  1:5337981/101‑1 (MQ=255)


                                                                              cGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgcc                        <  1:5030247/101‑1 (MQ=255)


                                                                               gTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgccg                       >  2:1784/1‑101 (MQ=255)


                                                                               gTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgccg                       >  1:3668582/1‑101 (MQ=255)


                                                                                 cAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCACGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCgccgcc                     >  2:4864326/1‑101 (MQ=255)


                                                                                  aaGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCt                    >  2:3417255/1‑101 (MQ=255)


                                                                                   aGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTc                   <  1:1280569/101‑1 (MQ=255)


                                                                                   aGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTc                   >  1:3465897/1‑101 (MQ=255)


                                                                                       cGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgc               >  2:3561089/1‑101 (MQ=255)


                                                                                        gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg              >  1:3266932/1‑101 (MQ=255)


                                                                                        gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg              >  1:7061853/1‑101 (MQ=255)


                                                                                         aaGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcgc             >  1:3932613/1‑101 (MQ=255)


                                                                                         aaGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcgc             >  1:2691586/1‑101 (MQ=255)


                                                                                          aGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGcgcg            >  2:432505/1‑101 (MQ=255)


                                                                                          aGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGcgcg            >  1:5951079/1‑101 (MQ=255)


                                                                                            cGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGt          >  1:1990115/1‑101 (MQ=255)


                                                                                             ggTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGtt         <  1:633856/101‑1 (MQ=255)


                                                                                              gTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtg        >  2:1850632/1‑101 (MQ=255)


                                                                                              gTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtg        <  1:1827487/101‑1 (MQ=255)


                                                                                                 gTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGa     <  1:4922776/101‑1 (MQ=255)


                                                                                                 gTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGa     >  2:2807878/1‑101 (MQ=255)


                                                                                                  tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt    >  1:2466854/1‑101 (MQ=255)


                                                                                                   aaaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATc   >  2:3210051/1‑101 (MQ=255)


                                                                                                   aaaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATc   >  1:829895/1‑101 (MQ=255)


                                                                                                    aaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATcc  <  2:5426963/101‑1 (MQ=255)


                                                                                                    aaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATcc  <  1:4753117/101‑1 (MQ=255)


AACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCC  >  NC_014034/2254718‑2254918

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG