Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,254,824 T→C 7.0% K14K (AAA→AAG)  RCAP_rcc02086 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,254,8240T→C 7.0% 54.6 ‑2.2 ‑1.3383K14K (AAA→AAG) RCAP_rcc02086hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/7):  ref base (206/150):  total (226/157)
Fisher's exact test strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.51e-02

Only 100 of 406 total aligned reads displayed.

ACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCC  >  NC_014034/2254724‑2254924
                                                                                                    |                                                                                                    
   ccccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTc                                                                                                   <  2:4021346/101‑1 (MQ=255)
       aaaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccc                                                                                               <  2:5638030/101‑1 (MQ=255)
       aaaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccc                                                                                               >  1:2386108/1‑101 (MQ=255)
       aaaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccc                                                                                               >  2:5487824/1‑101 (MQ=255)
          cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                                                            >  2:2355011/1‑101 (MQ=255)
           aGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAACGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAg                                                                                           <  1:5010926/101‑1 (MQ=255)
            gttGAACGCATGCCGTTTCCCCTTCGCTCGCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGc                                                                                          <  2:3558088/99‑1 (MQ=255)
            ggTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGc                                                                                          >  2:4716679/1‑101 (MQ=255)
             gTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCt                                                                                         >  2:4564368/1‑101 (MQ=255)
              tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCtt                                                                                        >  2:1525469/1‑101 (MQ=255)
                aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                                                      >  2:6753422/1‑101 (MQ=255)
                aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                                                      >  2:2436492/1‑101 (MQ=255)
                aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                                                      >  1:6008963/1‑101 (MQ=255)
                aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                                                      <  1:3698016/101‑1 (MQ=255)
                  cGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCgg                                                                                    <  2:2238019/101‑1 (MQ=255)
                     aTGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcgc                                                                                 <  1:2913132/101‑1 (MQ=255)
                      tGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGcgcg                                                                                <  1:3005829/101‑1 (MQ=255)
                        ccGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCggg                                                                              <  1:4222601/101‑1 (MQ=255)
                          gTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAt                                                                            <  2:1730538/101‑1 (MQ=255)
                          gTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGAGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAt                                                                            <  2:5253163/101‑1 (MQ=255)
                            ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                                                          >  1:335037/1‑101 (MQ=255)
                            ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                                                          <  2:5122240/101‑1 (MQ=255)
                              ccccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTcc                                                                        <  1:3585548/101‑1 (MQ=255)
                                tcgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgag                                                                      <  1:5794602/92‑1 (MQ=255)
                                ccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcag                                                                      <  1:4665966/101‑1 (MQ=255)
                                 cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                                     >  2:3359010/9‑100 (MQ=255)
                                 cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                                     <  2:4883317/93‑2 (MQ=255)
                                 cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                                     >  2:1418534/9‑100 (MQ=255)
                                 cTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagc                                                                     <  1:6732236/101‑1 (MQ=255)
                                 cTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagc                                                                     >  1:927765/1‑101 (MQ=255)
                                 cTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagc                                                                     <  1:4727168/101‑1 (MQ=255)
                                  ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCTAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                                    <  2:5754968/101‑1 (MQ=255)
                                  ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                                    >  1:3801503/1‑101 (MQ=255)
                                  ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                                    >  2:7007790/1‑101 (MQ=255)
                                  gatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGGCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGCCGCGGGATGTCgaggc                                                                    >  2:1240426/8‑99 (MQ=255)
                                   tCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagcag                                                                   >  2:4270025/1‑101 (MQ=255)
                                   tCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagcag                                                                   >  2:5939777/1‑101 (MQ=255)
                                   atcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggcc                                                                   >  1:7016466/7‑98 (MQ=255)
                                    cGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGt                                                                  <  2:5035180/101‑1 (MQ=255)
                                    cGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGt                                                                  >  1:227414/1‑101 (MQ=255)
                                     cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                                                 >  1:4048920/5‑96 (MQ=255)
                                     cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                                                 >  2:2949379/5‑96 (MQ=255)
                                      ctcgCGCCCTAAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCg                                                                <  2:1094503/101‑1 (MQ=255)
                                       tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                                                               <  2:6593058/101‑1 (MQ=255)
                                       tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTAAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                                                               <  1:4467002/101‑1 (MQ=255)
                                       cgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgc                                                               >  2:5378/3‑94 (MQ=255)
                                        gtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgca                                                              >  1:5107585/2‑93 (MQ=255)
                                        gtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgca                                                              >  1:3881156/2‑93 (MQ=255)
                                        gtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgca                                                              >  1:2477791/2‑93 (MQ=255)
                                        ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGCCGCa                                                              >  2:2747481/1‑101 (MQ=255)
                                           gCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAgg                                                           <  1:1686309/101‑1 (MQ=255)
                                             ccTCAATGGGCAGCGCCGAGGCGAAGGCGTNAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTg                                                         <  1:968218/101‑1 (MQ=255)
                                                  aTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTc                                                    >  2:6004707/1‑101 (MQ=255)
                                                   tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                                                   >  2:3149348/1‑101 (MQ=255)
                                                   tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                                                   <  1:4948352/101‑1 (MQ=255)
                                                   tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                                                   >  1:4870072/1‑101 (MQ=255)
                                                      gCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCagag                                                <  1:3497525/101‑1 (MQ=255)
                                                      gCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCagag                                                >  1:105281/1‑101 (MQ=255)
                                                       cAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAgaga                                               >  2:3235878/1‑101 (MQ=255)
                                                        aGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGAc                                              >  2:7094621/1‑101 (MQ=255)
                                                          cgcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGc                                            <  1:6352657/101‑1 (MQ=255)
                                                            ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa                                          <  2:2582807/101‑1 (MQ=255)
                                                            ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa                                          <  2:886119/101‑1 (MQ=255)
                                                             cGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCaa                                         <  2:6080206/101‑1 (MQ=255)
                                                             cGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCaa                                         >  1:5910677/1‑101 (MQ=255)
                                                                ggCGAAGGCGTCAAGGTCGAAGCGGTAGTAAAGACGTTTCGCCCACAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGt                                      >  1:7105456/1‑101 (MQ=255)
                                                                   gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                                   <  2:3002622/101‑1 (MQ=255)
                                                                   gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                                   <  2:785450/101‑1 (MQ=255)
                                                                     aGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATgc                                 >  1:2583688/1‑101 (MQ=255)
                                                                     aGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATgc                                 <  1:5337981/101‑1 (MQ=255)
                                                                      ggCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATgcg                                <  1:2707052/101‑1 (MQ=255)
                                                                         gTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgccg                             >  2:1784/1‑101 (MQ=255)
                                                                             aGGTCGATGCGGACGTAAAGACGTTGCGCCACCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTTAGAGACGCCAAGGTAGTGCgccgccgc                         <  2:4402165/101‑3 (MQ=255)
                                                                             aGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTc                         <  2:3577354/101‑1 (MQ=255)
                                                                             aGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTc                         <  1:1280569/101‑1 (MQ=255)
                                                                               gTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCgg                       >  2:2117129/1‑101 (MQ=255)
                                                                                tCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCggg                      <  2:4173567/101‑1 (MQ=255)
                                                                                 cGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgc                     >  2:6295370/1‑101 (MQ=255)
                                                                                 cGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgc                     >  1:5438183/1‑101 (MQ=255)
                                                                                  gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                    >  2:4426877/1‑101 (MQ=255)
                                                                                  gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                    >  1:15778/1‑101 (MQ=255)
                                                                                  gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                    >  1:1839182/1‑101 (MQ=255)
                                                                                   aaGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcgc                   >  2:6503566/1‑101 (MQ=255)
                                                                                    aGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGcgcg                  >  1:5697977/1‑101 (MQ=255)
                                                                                      cGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGt                >  2:3862187/1‑101 (MQ=255)
                                                                                      cGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGt                >  1:3935281/1‑101 (MQ=255)
                                                                                       ggTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGtt               >  1:6649825/1‑101 (MQ=255)
                                                                                       ggTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGtt               >  1:1461701/1‑101 (MQ=255)
                                                                                        gTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtg              >  2:1850632/1‑101 (MQ=255)
                                                                                        gTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtg              >  2:3427121/1‑101 (MQ=255)
                                                                                          cGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtgtg            >  2:476777/1‑101 (MQ=255)
                                                                                          cGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtgtg            >  1:6106906/1‑101 (MQ=255)
                                                                                           gTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGa           >  2:2807878/1‑101 (MQ=255)
                                                                                            tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt          <  1:2355106/101‑1 (MQ=255)
                                                                                            tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt          >  1:4769053/1‑101 (MQ=255)
                                                                                             aaaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATc         >  1:1995329/1‑101 (MQ=255)
                                                                                              aaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATcc        <  2:5426963/101‑1 (MQ=255)
                                                                                               aGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCg       >  1:627553/1‑101 (MQ=255)
                                                                                                  cGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGGGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGgca    >  1:4337050/1‑100 (MQ=255)
                                                                                                    tttCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGcc  <  1:279725/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
ACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCC  >  NC_014034/2254724‑2254924

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG