Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,254,830 C→T 7.0% G12G (GGG→GGA RCAP_rcc02086 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,254,8300C→T 7.0% 64.7 ‑2.2 ‑0.0385G12G (GGG→GGARCAP_rcc02086hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/7):  ref base (207/151):  total (227/158)
Fisher's exact test strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.24e-01

Only 100 of 419 total aligned reads displayed.

CAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCC  >  NC_014034/2254730‑2254930
                                                                                                    |                                                                                                    
  aaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGcccc                                                                                                    >  1:4944376/1‑101 (MQ=255)
  aaCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGcccc                                                                                                    >  1:4108435/1‑101 (MQ=255)
    cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAATGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                                                                  >  2:5252015/1‑101 (MQ=255)
    cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                                                                  >  1:2347680/1‑101 (MQ=255)
      ggTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGc                                                                                                >  2:4716679/1‑101 (MQ=255)
       gTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCt                                                                                               >  2:1827423/1‑101 (MQ=255)
        tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCtt                                                                                              <  2:3135689/101‑1 (MQ=255)
        tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCtt                                                                                              >  2:1525469/1‑101 (MQ=255)
        tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCtt                                                                                              <  1:2601489/101‑1 (MQ=255)
         gATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCttt                                                                                             >  2:2094734/1‑101 (MQ=255)
          aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                                                            >  2:1760659/1‑101 (MQ=255)
          aTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTc                                                                                            <  2:4262906/101‑1 (MQ=255)
             gCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgc                                                                                         >  2:4948100/1‑101 (MQ=255)
             gCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgc                                                                                         >  1:428408/1‑101 (MQ=255)
                 gCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCgg                                                                                     >  1:5818675/1‑101 (MQ=255)
                   cGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGa                                                                                   <  1:687892/101‑1 (MQ=255)
                     tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                                                                 <  2:231009/101‑1 (MQ=255)
                     tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                                                                 <  1:1315876/101‑1 (MQ=255)
                      ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                                                                >  2:54206/1‑101 (MQ=255)
                       tCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtctc                                                                               <  1:5931302/101‑1 (MQ=255)
                        ccccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTcc                                                                              >  1:2501327/1‑101 (MQ=255)
                         cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                                                             >  1:5281345/1‑101 (MQ=255)
                         cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                                                             >  1:949574/1‑101 (MQ=255)
                         cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                                                             <  1:6684116/101‑1 (MQ=255)
                          ccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcag                                                                            <  1:504287/101‑1 (MQ=255)
                           cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                                           >  2:1418534/9‑100 (MQ=255)
                           cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                                           >  1:4724342/9‑100 (MQ=255)
                            gatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggc                                                                          >  2:4761361/8‑99 (MQ=255)
                               gCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTc                                                                       <  1:1579767/101‑1 (MQ=255)
                               cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                                                       >  2:2949379/5‑96 (MQ=255)
                               cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                                                       >  2:6742983/5‑96 (MQ=255)
                                ctctCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCg                                                                      >  2:2175182/1‑101 (MQ=255)
                                 tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                                                                     >  1:3634363/1‑101 (MQ=255)
                                 cgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgc                                                                     <  2:7086579/99‑8 (MQ=255)
                                  ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCa                                                                    >  2:2569310/1‑101 (MQ=255)
                                    cGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAg                                                                  <  1:383012/101‑1 (MQ=255)
                                     gCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAgg                                                                 <  1:1686309/101‑1 (MQ=255)
                                        cTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGc                                                              >  2:3594150/1‑101 (MQ=255)
                                        cTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGc                                                              >  2:4393390/1‑101 (MQ=255)
                                         tCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCt                                                             <  1:4048914/101‑1 (MQ=255)
                                          cAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCtt                                                            <  2:5647914/101‑1 (MQ=255)
                                           aaTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCttt                                                           <  2:4538496/101‑1 (MQ=255)
                                            aTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTc                                                          >  1:5727685/1‑101 (MQ=255)
                                                ggaGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTGCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCagag                                                      <  1:2992840/99‑1 (MQ=255)
                                                gCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCagag                                                      >  1:105281/1‑101 (MQ=255)
                                                   gcgcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACg                                                   <  2:486874/101‑1 (MQ=255)
                                                    cgcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGc                                                  <  1:6352657/101‑1 (MQ=255)
                                                    cgcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGc                                                  >  2:6040707/1‑101 (MQ=255)
                                                      ccGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCa                                                <  2:886119/101‑1 (MQ=255)
                                                          ggCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGt                                            <  1:6086546/101‑1 (MQ=255)
                                                          ggCGAAGGCGTCAAGGTCGAAGCGGTAGTAAAGACGTTTCGCCCACAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGt                                            >  1:7105456/1‑101 (MQ=255)
                                                           gCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTa                                           <  2:1045587/101‑1 (MQ=255)
                                                           gCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTa                                           <  1:1957193/101‑1 (MQ=255)
                                                            cGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTaa                                          <  2:578633/101‑1 (MQ=255)
                                                             gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGCGACGCCAAGGTAAt                                         >  1:2818610/1‑101 (MQ=255)
                                                             gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                                         <  2:785450/101‑1 (MQ=255)
                                                             gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                                         <  2:5117535/101‑1 (MQ=255)
                                                              aaGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGAAATg                                        >  2:3321207/1‑101 (MQ=255)
                                                              aaGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGGGCTTTCAGAGACGCCAAGGTAATg                                        >  1:2376084/1‑101 (MQ=255)
                                                                 gCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATgcgc                                     >  2:4071591/1‑101 (MQ=255)
                                                                  cGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgcc                                    >  1:2737840/1‑101 (MQ=255)
                                                                    tCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgccgc                                  >  2:3423690/1‑101 (MQ=255)
                                                                     cAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCgccgcc                                 >  2:2401627/1‑101 (MQ=255)
                                                                      aaGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCGAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCt                                >  1:5005886/1‑101 (MQ=255)
                                                                      aaGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCt                                >  2:3561556/1‑101 (MQ=255)
                                                                       aGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTc                               >  1:4459328/1‑101 (MQ=255)
                                                                       aGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTc                               >  1:3465897/1‑101 (MQ=255)
                                                                           cGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgc                           <  2:5949112/101‑1 (MQ=255)
                                                                            gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                          >  1:3540795/1‑101 (MQ=255)
                                                                            gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                          >  2:3213669/1‑101 (MQ=255)
                                                                            gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                          >  1:3266932/1‑101 (MQ=255)
                                                                             aaGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcgc                         >  1:3932613/1‑101 (MQ=255)
                                                                             aaGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcgc                         >  2:5469584/1‑101 (MQ=255)
                                                                              aGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGcgcg                        >  1:3735019/1‑101 (MQ=255)
                                                                                cGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGt                      >  2:3862187/1‑101 (MQ=255)
                                                                                cGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGt                      >  1:1188661/1‑101 (MQ=255)
                                                                                 ggTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGtt                     >  2:2669038/1‑101 (MQ=255)
                                                                                  gTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtg                    >  1:3352594/1‑101 (MQ=255)
                                                                                   tCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCGGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtgt                   <  2:6521007/101‑1 (MQ=255)
                                                                                    cGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtgtg                  >  2:6401835/1‑101 (MQ=255)
                                                                                     gTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGa                 >  2:2807878/1‑101 (MQ=255)
                                                                                     gTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGa                 >  2:6112379/1‑101 (MQ=255)
                                                                                     gTAAAGACGTTTCGCCCCCAGCTTTCGGCGCCGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTACTGCGCTGTCTCGGGCGCGGGTGTGa                 >  2:1236359/1‑101 (MQ=255)
                                                                                       aaaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATc               >  1:454837/1‑101 (MQ=255)
                                                                                        aaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATcc              <  1:4753117/101‑1 (MQ=255)
                                                                                         aGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCg             >  1:627553/1‑101 (MQ=255)
                                                                                            cGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGgcg          >  2:4422840/1‑101 (MQ=255)
                                                                                              tttCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGcc        >  1:5475459/1‑101 (MQ=255)
                                                                                               ttCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCa       >  2:1672476/1‑101 (MQ=255)
                                                                                                tCGCCCCCAGCTTTCGGCGCTGGATCGCCAGCAGTCGCAAGGTGCTTTCAGAGGCGCCAAGGTAATGCTCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAg      <  1:5390929/101‑1 (MQ=255)
                                                                                                tCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAg      <  1:6617294/101‑1 (MQ=255)
                                                                                                tCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAg      <  2:3508456/101‑1 (MQ=255)
                                                                                                 cGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAgg     >  1:2024725/1‑101 (MQ=255)
                                                                                                 cGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAgg     <  1:6003963/101‑1 (MQ=255)
                                                                                                  gCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGt    >  2:6987726/1‑101 (MQ=255)
                                                                                                   cccccAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTc   >  1:1492919/1‑101 (MQ=255)
                                                                                                   cccccAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTc   <  2:5334028/101‑1 (MQ=255)
                                                                                                    ccccCGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTcc  <  1:2273780/101‑1 (MQ=255)
                                                                                                    ccccAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTcc  >  1:4855905/1‑101 (MQ=255)
                                                                                                    ccccAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTcc  >  1:854890/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCC  >  NC_014034/2254730‑2254930

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG