Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,580 G→T 100% T57K (ACG→AAG)  RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,5800G→T100.0% 1335.5 369T57K (ACG→AAG) RCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (224/145):  ref base (0/0):  total (224/145)

Only 100 of 407 total aligned reads displayed.

CTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGG  >  NC_014034/2262482‑2262676
                                                                                                  |                                                                                                
cTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTc                                                                                                <  2:5081993/101‑1 (MQ=255)
cTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTc                                                                                                <  1:379204/101‑1 (MQ=255)
 ccGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCa                                                                                               <  2:5841422/100‑1 (MQ=255)
  cGCAATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTGTCTTCCTCAAACTGCTTCAg                                                                                              >  1:4366915/1‑101 (MQ=255)
    ccATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCCAACTGCTTCAgcc                                                                                            >  2:6720528/1‑100 (MQ=255)
     cATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccg                                                                                           <  1:2675565/101‑3 (MQ=255)
     cATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccg                                                                                           >  1:2469458/1‑99 (MQ=255)
      aTTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACAATCGCCACCAGGCGGTTCAGCTTGGCGTTGTCTTACTCCAACTGCTTCAgccgc                                                                                          >  2:1498153/1‑101 (MQ=14)
       ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                         >  1:1109832/1‑101 (MQ=255)
        tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                        <  1:4169341/101‑1 (MQ=255)
        tGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCtt                                                                                        >  2:2338893/1‑101 (MQ=255)
         gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                       >  1:1408125/1‑101 (MQ=255)
         gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                       <  2:4150241/101‑1 (MQ=255)
           gCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGc                                                                                     <  1:22062/101‑1 (MQ=255)
            cTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCa                                                                                    >  1:5372087/1‑101 (MQ=255)
             ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAt                                                                                   <  2:3273822/101‑1 (MQ=255)
              tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                  >  2:4441155/1‑101 (MQ=255)
              tGAACCAACGTCGCCCCACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                  <  1:3362208/101‑1 (MQ=17)
                aaCCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCgg                                                                                <  2:6459421/101‑1 (MQ=255)
                  ccAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGAc                                                                              >  1:6962133/1‑101 (MQ=255)
                  ccAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGAc                                                                              <  2:5184750/101‑1 (MQ=255)
                  ccAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTGCCTCAAACTGCTTCAGCCGCTTGGCATCGGAc                                                                              >  2:3897655/1‑101 (MQ=255)
                   cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                             >  2:1400617/1‑101 (MQ=255)
                   cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                             <  2:6157864/101‑1 (MQ=255)
                   cAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACa                                                                             >  2:1675398/1‑101 (MQ=255)
                    aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                            >  1:3751679/1‑101 (MQ=255)
                     aTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaaa                                                                           <  1:2378914/101‑1 (MQ=255)
                      tGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGCCAAat                                                                          >  2:2841242/1‑101 (MQ=255)
                      tGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAat                                                                          <  1:5152004/101‑1 (MQ=255)
                        gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                        <  1:4890074/101‑1 (MQ=255)
                        gcgcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatcc                                                                        <  2:6282795/101‑1 (MQ=255)
                          gcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccat                                                                      <  2:3621662/101‑1 (MQ=255)
                           ccACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatc                                                                     <  1:910460/101‑1 (MQ=255)
                                 tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                               >  2:2030117/1‑101 (MQ=255)
                                  ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                              >  1:3059271/1‑101 (MQ=255)
                                  ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                              >  2:5735371/1‑101 (MQ=255)
                                   gCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                             >  2:6543142/1‑101 (MQ=255)
                                   gCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                             >  1:3582205/1‑101 (MQ=255)
                                   gCTCGATCCAGCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                             <  2:3768874/101‑1 (MQ=18)
                                    cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                            <  1:6736161/101‑1 (MQ=255)
                                     tCGATCCAGCATCACATCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACt                                                           <  2:4013441/101‑1 (MQ=18)
                                     tCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACt                                                           >  2:5972468/1‑101 (MQ=255)
                                      cGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                          >  2:4591268/1‑101 (MQ=255)
                                      cGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                          >  1:552660/1‑101 (MQ=255)
                                      cGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACtt                                                          >  2:3430003/1‑101 (MQ=255)
                                       gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                         >  2:1129411/1‑101 (MQ=255)
                                        aTCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTgg                                                        >  1:1755287/1‑101 (MQ=255)
                                          ccAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGcc                                                      >  2:4799232/1‑101 (MQ=255)
                                            accATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCtt                                                    >  1:6816031/3‑101 (MQ=21)
                                            aGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCtt                                                    <  1:6386467/101‑1 (MQ=255)
                                             gCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTc                                                   <  2:3867962/101‑1 (MQ=18)
                                             gCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTc                                                   <  1:525285/101‑1 (MQ=18)
                                              cATCACTTCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCa                                                  <  2:131764/101‑1 (MQ=18)
                                               aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                 <  2:2238960/101‑1 (MQ=255)
                                                tcccacccGCCACCGGGCGCTTCAGGTTGGCTTTCTCTTCCTCAACCTCCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGc                                                <  1:6202029/95‑1 (MQ=18)
                                                tCACATCCGCCCCCAGGCCCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGc                                                <  1:2949747/101‑1 (MQ=18)
                                                   caTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTg                                             <  2:3366649/101‑1 (MQ=255)
                                                    ctCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                            <  2:497843/100‑1 (MQ=255)
                                                    aTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                            >  1:3481030/1‑101 (MQ=255)
                                                    aTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                            >  1:6584007/1‑101 (MQ=255)
                                                     tCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                           <  1:999089/101‑1 (MQ=18)
                                                     cccGCCACCAGGCGCTTCAGTTTGCCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                           <  2:4311030/100‑1 (MQ=18)
                                                       cGCCACCAGGCGCTTCAGCTGGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGa                                         <  2:76055/101‑1 (MQ=18)
                                                          caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                      >  2:1735534/1‑101 (MQ=255)
                                                             caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                   >  1:4729898/1‑101 (MQ=255)
                                                              aGGCGCTTCAGCTTGGCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgc                                  <  1:2841344/101‑1 (MQ=18)
                                                                  gcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                              >  2:2997459/1‑101 (MQ=255)
                                                                  gcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                              >  1:714378/1‑101 (MQ=255)
                                                                   cTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGc                             >  1:1147449/1‑101 (MQ=255)
                                                                     tCAGCTTGGTGGCCCCTCCCTAAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                           <  2:274254/101‑1 (MQ=18)
                                                                     tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGGGGGGCTg                           >  2:4518954/1‑101 (MQ=25)
                                                                     tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                           >  1:3918816/1‑101 (MQ=255)
                                                                      cAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                          >  2:5683005/1‑101 (MQ=255)
                                                                      cAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGa                          >  1:213035/1‑101 (MQ=255)
                                                                        gCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATACATCCCCCCATACTTCGCCTTCAGCTTTTAGAAGGTCGCGGGGCTGAGc                        >  1:3274769/1‑101 (MQ=255)
                                                                        gCTTGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGc                        <  1:5930327/101‑1 (MQ=21)
                                                                         cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                       >  1:4593518/1‑101 (MQ=255)
                                                                         cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGACNTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                       >  1:3213954/1‑101 (MQ=21)
                                                                          ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                      <  1:5829886/101‑1 (MQ=255)
                                                                          ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                      <  2:3582069/101‑1 (MQ=255)
                                                                          ttGGCGTCCTCTCCCTCAAACTGCTCCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                      <  1:791844/101‑1 (MQ=21)
                                                                           tGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                     >  2:6900824/1‑101 (MQ=25)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc               >  1:4461975/1‑101 (MQ=255)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc               >  2:2538395/1‑101 (MQ=37)
                                                                                 tctcTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTc               >  1:1037785/1‑101 (MQ=37)
                                                                                  ctctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTcc              >  2:1604959/1‑101 (MQ=37)
                                                                                   tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg             >  1:6119067/1‑101 (MQ=37)
                                                                                   tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg             >  2:5472303/1‑101 (MQ=37)
                                                                                     ttCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGc           >  1:5733411/1‑101 (MQ=25)
                                                                                       ccTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaa         >  2:4642377/1‑101 (MQ=255)
                                                                                        cTCAAAGTGCTCCAGCCGTTTGCCATCGAACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaaa        <  2:1895398/101‑1 (MQ=21)
                                                                                        cTCAAACTGCTTCAGCCGTTTTGCTTCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaaa        <  2:7012178/101‑1 (MQ=21)
                                                                                        cTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaaa        >  1:1571167/1‑101 (MQ=255)
                                                                                          cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc      >  1:3662455/1‑101 (MQ=255)
                                                                                            aaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc    <  1:646966/101‑1 (MQ=255)
                                                                                            aaCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTc    >  1:6701786/1‑101 (MQ=255)
                                                                                             aCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCg   >  1:4985808/1‑101 (MQ=255)
                                                                                             aCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCg   >  2:2622467/1‑101 (MQ=255)
                                                                                              cTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg  <  2:5371815/101‑1 (MQ=255)
                                                                                              cTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg  >  1:1848980/1‑101 (MQ=255)
                                                                                                  |                                                                                                
CTCGCCATTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGG  >  NC_014034/2262482‑2262676

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG