Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,467,061 G→C 100% V29L (GTC→CTC)  RCAP_rcc02292 → signal transduction histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,467,0610G→C100.0% 1160.6 313V29L (GTC→CTC) RCAP_rcc02292signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  new base (136/176):  ref base (1/0):  total (137/176)

Only 100 of 320 total aligned reads displayed.

GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158
                                                                                                  |                                                                                                 
 ggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCa                                                                                                <  1:3841014/101‑1 (MQ=255)
 ggCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCa                                                                                                <  2:4570137/101‑1 (MQ=255)
 ggCCGCAGGCGGCATGATCCGGGGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCa                                                                                                <  2:531585/101‑1 (MQ=255)
  gcccgaGGCGGTACGATCCGGTGGCGACGCGGCCGGGGGGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAg                                                                                               <  2:6409803/96‑1 (MQ=255)
     gCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTa                                                                                            <  1:5118169/101‑1 (MQ=255)
     gCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTa                                                                                            <  2:83152/101‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGGGGTGCTGAGGCAGCTCGCCGTGCTCAGCTAt                                                                                           >  2:2272439/1‑101 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  2:305836/101‑1 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           >  1:3001186/1‑101 (MQ=255)
      cAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTAt                                                                                           <  2:2154216/101‑1 (MQ=255)
       aGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGAGGTGCTGACGCCGCTCGCCGTGCTCAGCTATg                                                                                          >  1:4741795/1‑101 (MQ=255)
        ggcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgc                                                                                         >  1:5675745/1‑101 (MQ=255)
         gcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcg                                                                                        <  2:4138119/101‑1 (MQ=255)
          cggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcgc                                                                                       >  2:2533489/1‑101 (MQ=255)
           ggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCa                                                                                      >  2:2622854/1‑101 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  <  1:6689820/101‑1 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  <  1:4069709/101‑1 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCAGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                  >  1:5257012/1‑101 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                               <  2:2001659/101‑1 (MQ=255)
                     ggTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                            <  1:835069/101‑1 (MQ=255)
                     ggTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                            >  1:1682306/1‑101 (MQ=255)
                     ggTGGAGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTTTCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                            <  1:3989116/101‑1 (MQ=255)
                       tGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAc                                                                          >  2:6116299/1‑101 (MQ=255)
                         gCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCg                                                                        <  1:6578262/101‑1 (MQ=255)
                          cGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGa                                                                       >  1:817007/1‑101 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCAGGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGac                                                                      >  2:3393467/1‑100 (MQ=255)
                            aCGTGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAAc                                                                     <  1:6287073/101‑1 (MQ=255)
                            aCGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAAc                                                                     >  2:1331885/1‑101 (MQ=255)
                             cgcgGCACGGGCGGTCTGGGCCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACg                                                                    <  1:4351176/101‑1 (MQ=255)
                               cgGCACGGGCGGTCTGGGGCTGCGGCTAACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACggg                                                                  >  1:1024334/1‑101 (MQ=255)
                                ggCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgc                                                                 >  1:5870542/1‑101 (MQ=255)
                                ggCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgc                                                                 >  2:1378414/1‑101 (MQ=255)
                                ggCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgc                                                                 >  1:3608646/1‑101 (MQ=255)
                                 gCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgcc                                                                <  2:2026716/101‑1 (MQ=255)
                                  cACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgccg                                                               >  2:3290796/1‑101 (MQ=255)
                                    cGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGgccgcc                                                             <  1:5958776/101‑1 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGccgccg                                                            <  1:6849812/101‑1 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGccgccg                                                            >  1:6726273/1‑101 (MQ=255)
                                      ggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCTGCCGa                                                           <  2:4840924/101‑1 (MQ=255)
                                      ggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGa                                                           <  2:5161665/101‑1 (MQ=255)
                                      ggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGa                                                           >  2:5568386/1‑101 (MQ=255)
                                       gCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaa                                                          <  1:5402472/101‑1 (MQ=255)
                                        gggTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaa                                                         <  1:3368508/100‑1 (MQ=255)
                                         ggTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGaaaa                                                        >  1:6615915/1‑101 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      >  1:4829914/1‑101 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      <  1:2010021/101‑1 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      <  1:3851849/101‑1 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAAcc                                                      >  1:6171494/1‑101 (MQ=255)
                                            ttGGGGCTGGCGGTGGCGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCg                                                     <  2:1706927/100‑1 (MQ=255)
                                            cTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCg                                                     >  2:5816538/1‑101 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGGCCCTGGCCACCGAACGGGCCGCCGAAAACCGt                                                    >  1:1919701/1‑101 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCgc                                                    >  1:2302225/1‑100 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGt                                                    >  1:6423897/1‑101 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGt                                                    <  2:1652709/101‑1 (MQ=255)
                                              ggggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTc                                                   >  1:1216584/1‑101 (MQ=255)
                                               gggCTGCGGCTGGCGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  <  1:7091252/101‑1 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  <  1:1384337/101‑1 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCt                                                  <  2:376332/101‑1 (MQ=255)
                                                ggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTg                                                 <  2:5884470/101‑1 (MQ=255)
                                                 gCTGCGTCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGCCCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgc                                                <  2:1486066/101‑1 (MQ=255)
                                                 gCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgc                                                >  1:1093490/1‑101 (MQ=255)
                                                  cTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTgcg                                               <  1:2533583/101‑1 (MQ=255)
                                                             ggTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATcc                                    >  1:1433023/1‑101 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   <  2:5519040/101‑1 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   >  2:3535163/1‑101 (MQ=255)
                                                               tGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTg                                  >  2:4034640/1‑101 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 <  1:2211808/101‑1 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 <  2:4401708/101‑1 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 <  2:5732341/101‑1 (MQ=255)
                                                                  tCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGc                               <  1:5306581/101‑1 (MQ=255)
                                                                    aTGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCga                             >  1:2615763/1‑101 (MQ=255)
                                                                     tGGGGGCGTTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgag                            <  1:3487552/101‑1 (MQ=255)
                                                                     tGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgag                            >  1:279148/1‑101 (MQ=255)
                                                                      gggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgagc                           >  2:4253604/1‑100 (MQ=255)
                                                                       ggggCGGTGCTGACGCCGCTCGCCGTGCTCATCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGAc                          <  2:260133/101‑1 (MQ=255)
                                                                        gggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACg                         <  2:6333885/101‑1 (MQ=255)
                                                                        gggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACg                         <  1:1576272/101‑1 (MQ=255)
                                                                         ggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCCGCGATCCTGGGCGAGACGc                        >  1:3820416/1‑101 (MQ=255)
                                                                          gCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCt                       <  2:1175900/101‑1 (MQ=255)
                                                                            ggTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGa                     <  2:279144/101‑1 (MQ=255)
                                                                             gTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGAt                    <  2:5675455/101‑1 (MQ=255)
                                                                              tGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATg                   <  2:6673926/101‑1 (MQ=255)
                                                                                cTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgc                 <  1:1796896/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  2:1787261/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  2:2997737/101‑1 (MQ=255)
                                                                                   acccatgccGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgccgc              >  1:498144/9‑101 (MQ=255)
                                                                                     cttccgatctgTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccg            <  1:870875/91‑1 (MQ=255)
                                                                                       cgcTCGCCGTGCTCAGCTATGCGCAGGCCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcg          <  2:1919629/101‑1 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        >  2:460177/1‑101 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        <  1:2650493/101‑1 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        >  1:4572385/1‑101 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        <  2:7000745/101‑1 (MQ=255)
                                                                                         cTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgcc        <  2:718292/101‑1 (MQ=255)
                                                                                           cGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCgccgcgccgc      <  2:4354890/101‑1 (MQ=255)
                                                                                            gCCGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcgccgag     <  2:4945821/101‑3 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAg    <  2:4741555/101‑1 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAg    <  2:3872043/101‑1 (MQ=255)
                                                                                             ccGTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAg    <  2:3231348/101‑1 (MQ=255)
                                                                                               gTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccgagatcggaag  >  2:870851/1‑91 (MQ=255)
                                                                                               gTGCTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAggc  >  2:504293/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCGAACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG