Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,481,061 T→G 100% E322A (GAG→GCG)  RCAP_rcc02306 ← LysM domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,481,0610T→G100.0% 492.2 135E322A (GAG→GCG) RCAP_rcc02306LysM domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (120/15):  ref base (0/0):  total (120/15)

Only 100 of 158 total aligned reads displayed.

GCGGCGGGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCCCATCAAGGCGCATCGCATGCGGCCCGGGCGTCTCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGCCCCCTTGGG  >  NC_014034/2480963‑2481158
                                                                                                  |                                                                                                 
 cggcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGGCGCcg                                                                                                >  2:2579362/1‑101 (MQ=255)
   gcgggGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCcgcg                                                                                              <  1:3489951/101‑1 (MQ=255)
      gggCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGc                                                                                           >  1:1117079/1‑101 (MQ=255)
         cGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGc                                                                                        >  2:336488/1‑101 (MQ=255)
          gACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGcc                                                                                       <  2:769908/101‑1 (MQ=255)
          gACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGcc                                                                                       >  2:6984875/1‑101 (MQ=255)
          gACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGcc                                                                                       >  1:3097621/1‑101 (MQ=255)
          gACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGcc                                                                                       >  1:462582/1‑101 (MQ=255)
           aCGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCg                                                                                      >  2:6741146/1‑101 (MQ=255)
             gCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCa                                                                                    >  1:4067071/1‑101 (MQ=255)
             gCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCa                                                                                    <  1:4257909/101‑1 (MQ=255)
             gCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCa                                                                                    >  1:4509091/1‑101 (MQ=255)
             gCCCTCGAAGCCCTGATCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTGGGCGCGGGCAGCCGCa                                                                                    >  1:3289276/1‑101 (MQ=255)
              cccTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAg                                                                                   >  2:6093930/1‑101 (MQ=255)
               ccTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGc                                                                                  >  2:5055451/1‑101 (MQ=255)
                  cGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTg                                                                               <  1:6741500/101‑1 (MQ=255)
                   gAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGa                                                                              >  2:3166394/1‑101 (MQ=255)
                    aaGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGaa                                                                             >  2:2953974/1‑101 (MQ=255)
                    aaGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGaa                                                                             >  1:1457037/1‑101 (MQ=255)
                     aGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGaaa                                                                            <  1:18352/101‑1 (MQ=255)
                         cTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccgc                                                                        >  1:187918/1‑101 (MQ=255)
                           gCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCCGCGTGAAAccgccg                                                                      >  2:2764814/1‑101 (MQ=255)
                           gCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAccgccg                                                                      >  1:3215100/1‑101 (MQ=255)
                            cTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGt                                                                     >  1:3048167/1‑101 (MQ=255)
                             tCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTc                                                                    >  2:4352188/1‑101 (MQ=255)
                              cGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTcc                                                                   >  1:7112045/1‑101 (MQ=255)
                              cGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTcc                                                                   >  2:4175029/1‑101 (MQ=255)
                               ggCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGGccc                                                                  >  2:747006/1‑101 (MQ=255)
                                gcgcGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTcccc                                                                 <  2:6469064/101‑1 (MQ=255)
                                gcgcGGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTcccc                                                                 <  1:6465943/101‑1 (MQ=255)
                                 cgcgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccg                                                                >  2:3587923/1‑101 (MQ=255)
                                 cgcgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccg                                                                >  2:4876229/1‑101 (MQ=255)
                                  gcgGGCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgc                                                               >  2:499637/1‑101 (MQ=255)
                                    gggCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGTGCAGCCGCAGCGTGAAACCGCCGTCcccgccc                                                             >  1:3629081/1‑101 (MQ=255)
                                    gggCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgccc                                                             >  2:2096445/1‑101 (MQ=255)
                                    gggCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCcccgccc                                                             >  1:6897507/1‑101 (MQ=255)
                                      gCCAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCccgcccgc                                                           >  2:5498853/1‑101 (MQ=255)
                                       ccAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCcgcccgcc                                                          >  1:5289145/1‑101 (MQ=255)
                                        cAGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCt                                                         >  1:4945511/1‑101 (MQ=255)
                                         aGCACCCGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCCCCCGCCtc                                                        >  1:2281660/1‑100 (MQ=255)
                                          ccccccGCCCCGGGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGc                                                       <  2:4874322/98‑1 (MQ=255)
                                             cccGGCCCTCGGCATCGAGCGCATCAGGGCGCAGCGCATGCGTCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCAcc                                                    <  2:801939/101‑1 (MQ=255)
                                             cccGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCAcc                                                    >  2:5634067/1‑101 (MQ=255)
                                               cGGCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCaa                                                  >  1:2339765/1‑101 (MQ=255)
                                                ggCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCacc                                                 >  2:3180665/1‑99 (MQ=255)
                                                 gCCCGCGGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAcc                                                >  1:6512742/1‑101 (MQ=255)
                                                  cccGCGGCATCGAGCGCATCAAGGCGCAGCGCAGGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAAccc                                               >  1:3708901/1‑101 (MQ=255)
                                                    cgcgGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCa                                             <  1:2579458/101‑1 (MQ=255)
                                                     gcgGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCAc                                            >  1:1276568/1‑101 (MQ=255)
                                                      cgGCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAAGCGCCGTCCCCGCCCGCCTGCACCAACCCCAcc                                           >  1:3411835/1‑101 (MQ=255)
                                                        gCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTc                                         >  2:1222857/1‑101 (MQ=255)
                                                        gCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTc                                         >  2:1413762/1‑101 (MQ=255)
                                                        gCATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTc                                         >  2:523591/1‑101 (MQ=255)
                                                         cATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCCGCCGCAGCGTGAAACCGGCGTCCCCGCCCGCCTGCACCACACCCACCTCg                                        >  2:573639/1‑101 (MQ=255)
                                                         cATCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCg                                        >  1:4198602/1‑101 (MQ=255)
                                                          aTCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCgg                                       >  2:2061658/1‑101 (MQ=255)
                                                          aTCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCgg                                       >  1:6353862/1‑101 (MQ=255)
                                                           tCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGc                                      >  2:2523209/1‑101 (MQ=255)
                                                           tCGAGCGCATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCCGc                                      >  2:7065736/1‑101 (MQ=255)
                                                             gAGCGCATCAAGGCGCAGCGCAGGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCAt                                    <  1:6985248/101‑1 (MQ=255)
                                                              ggcgcATCAGGGCGCAGCGCTTGCGGCCGGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCAtt                                   <  2:2339670/97‑1 (MQ=255)
                                                                 gcATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTc                                >  2:3795646/2‑101 (MQ=255)
                                                                  cATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCg                               >  2:5921952/1‑101 (MQ=255)
                                                                  cATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCg                               >  1:2699322/1‑101 (MQ=255)
                                                                  cATCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGAAGCGTGAAACAGCCGTCCCCGCCCGCCTGCAACAACCCCACCTCGGCACTGTCg                               >  2:1112850/1‑101 (MQ=255)
                                                                   aTCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCgc                              >  2:2011205/1‑100 (MQ=255)
                                                                   aTCAAGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGa                              >  1:2910439/1‑101 (MQ=255)
                                                                    gcAAGGCGCAGCTCATGCGGCCCGGGCGTCGCGGCGGGCAGCCGCAGCGTGAACCCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGAc                             <  1:4810363/100‑1 (MQ=255)
                                                                      aaGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACAt                           >  2:2958452/1‑101 (MQ=255)
                                                                      aaGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACAt                           >  2:2861759/1‑101 (MQ=255)
                                                                       aGGCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATa                          <  1:2942888/101‑1 (MQ=255)
                                                                        ggCGCAGCGCGTGCGCCCCGGGCCTCGCCGCGGGCAGCCCCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAg                         <  2:3289160/101‑1 (MQ=255)
                                                                        ggCGCAGCGCATGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAg                         >  2:1706229/1‑101 (MQ=255)
                                                                          cgcagcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAACCCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgc                       <  1:5221939/101‑1 (MQ=255)
                                                                           gcagcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgcg                      >  1:3925963/1‑101 (MQ=255)
                                                                           gcagcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgcg                      >  2:579131/1‑101 (MQ=255)
                                                                             agcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCGGCCTGCACCAACCCCACCTCGGCATTGTCGGCATAGgcgcg                    >  1:3339214/3‑101 (MQ=255)
                                                                             agcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgcgcg                    >  2:5179685/3‑101 (MQ=255)
                                                                              gcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgcgcgc                   <  1:1171509/100‑1 (MQ=255)
                                                                              gcgcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGgcgcgc                   <  2:4508852/100‑1 (MQ=255)
                                                                                gcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAg                 >  2:6438094/1‑101 (MQ=255)
                                                                                gcatGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATCGTCGGCATAgatcggaag                 >  1:915410/1‑93 (MQ=255)
                                                                                  atGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGAGCTAGGAGCGAAGGa               >  2:2826823/1‑101 (MQ=255)
                                                                                  atGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGa               >  1:1297124/1‑101 (MQ=255)
                                                                                  atGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGa               >  2:242652/1‑101 (MQ=255)
                                                                                   tGCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAg              >  2:6471183/1‑101 (MQ=255)
                                                                                   tGCGCCCCGGGCGTCGCCGCGGCCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAg              <  1:1423811/101‑1 (MQ=255)
                                                                                    gCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAACCCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAgc             <  2:3960375/101‑1 (MQ=255)
                                                                                    gCGGCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAgc             <  1:6162951/101‑1 (MQ=255)
                                                                                      ggCCGGGGCGCCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAgcgc           <  1:2096525/101‑1 (MQ=255)
                                                                                      ggCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAgcgc           >  2:4312005/1‑101 (MQ=255)
                                                                                      ggCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAgcgc           >  2:3743511/1‑101 (MQ=255)
                                                                                       gCCCGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGcc          >  2:5182073/1‑101 (MQ=255)
                                                                                         ccGGGCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGcccc        >  2:1487281/1‑101 (MQ=255)
                                                                                         ccGGGCGCCGCCGCGGGCAGCCGCAGCGTGAACCCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGcccc        <  2:3839986/101‑1 (MQ=255)
                                                                                           gggCGCCGCCGCGGGCAGCCGCAGCGTGAACCCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGCCCCCt      <  1:242656/101‑1 (MQ=255)
                                                                                            ggCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGCCCCCtt     >  2:3493491/1‑101 (MQ=255)
                                                                                             gCGTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGCCCCCTTg    >  2:616053/1‑101 (MQ=255)
                                                                                               gTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGCCCCCTTggg  >  2:6170574/1‑101 (MQ=255)
                                                                                               gTCGCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGCCCCCTTggg  >  2:3664170/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGGCGGGGCGACGCCCTCGAAGCCCTGCTCGGCGCGGGCCAGCACCCGGCCCGCGGCATCGAGCCCATCAAGGCGCATCGCATGCGGCCCGGGCGTCTCCGCGGGCAGCCGCAGCGTGAAACCGCCGTCCCCGCCCGCCTGCACCAACCCCACCTCGGCATTGTCGACATAGGCGCGCAGGAGCGCCCCCTTGGG  >  NC_014034/2480963‑2481158

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG