Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,003 A→G 4.0% intergenic (+51/‑191) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0030A→G 4.0% 18.9 ‑1.0 ‑0.0298intergenic (+51/‑191)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (105/181):  total (111/187)
Fisher's exact test strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.71e-01

Only 100 of 318 total aligned reads displayed.

GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.A.GGGG.GGGCGCTG.CCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGT  >  NC_014034/2493904‑2494101
                                                                                                        |                                                                                                       
gcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgca                                                                                                         <  1:7127116/101‑1 (MQ=25)
    cTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCaa                                                                                                     <  1:5399307/101‑1 (MQ=25)
    cTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCaa                                                                                                     <  2:4498781/101‑1 (MQ=25)
     tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                                                                                    <  2:1348650/101‑1 (MQ=21)
      ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                                                                                   >  1:4125887/1‑101 (MQ=21)
        cccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATg                                                                                                 <  2:2959531/101‑1 (MQ=21)
        cccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATg                                                                                                 <  1:6930124/101‑1 (MQ=21)
         ccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGa                                                                                                >  1:1978802/1‑101 (MQ=21)
          cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                                                                               <  2:525570/101‑1 (MQ=21)
           aGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGATGaaa                                                                                              >  1:4687584/1‑101 (MQ=21)
           aGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                              <  1:3984449/101‑1 (MQ=21)
           aGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                                                              <  2:6685182/101‑1 (MQ=21)
            gcgcCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                                                             <  2:6926121/101‑1 (MQ=21)
              gccgcGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                           <  1:6574276/101‑1 (MQ=21)
               ccgcGGCGGGCGCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                          <  1:3066748/101‑1 (MQ=21)
                 gcggcgGGCGCGCGGCACGACAGGCCGGGGAA.A.GGGG.GGGGGCTG.CCCCCCGTCCGGCAGCGGGCTCCCCCCCGGCAATTTGCCGCACAAAGAAACCGGaa                                                                                        >  1:1170684/1‑100 (MQ=255)
                    gcgggcgCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                     <  2:1668214/101‑1 (MQ=21)
                     cgggcgCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                    <  1:3704793/101‑1 (MQ=21)
                      gggcgCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGGGCCGCCCCCCGGCCCGCAAGCGGCCTCCCCCCGGGAAATTTGCGCAAAGAGAAAACCGGAGGCCt                                                                                    >  2:5345353/1‑101 (MQ=255)
                      gggcgCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                   <  1:3236547/101‑1 (MQ=21)
                      gggccCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                   <  2:6566675/101‑1 (MQ=18)
                       ggcgCGCGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                  >  1:5868744/1‑101 (MQ=21)
                        gcgcgcGGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                 <  2:1128414/101‑1 (MQ=21)
                          gcgggGCCCGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                               <  1:6050482/101‑1 (MQ=18)
                          gcgcgGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                               <  1:5963658/101‑1 (MQ=21)
                          gcgcgGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                               <  1:4183156/101‑1 (MQ=21)
                           cgcgGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                              <  1:4354939/101‑1 (MQ=21)
                             cgGCACGACAGGCC.GAGTA.AGGGGG.GGGCGGTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAACCGATGCATTTCAttct                                                                            >  2:3292121/1‑98 (MQ=17)
                             cgGCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCCTCCGCAAGCGGACTCCCCCCGGGATATTTTCAGCAAGATGAAACCCGATGCCTTTCATCtt                                                                            >  1:2441510/1‑101 (MQ=18)
                              ggCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                           >  1:5687611/1‑101 (MQ=21)
                              ggCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                           <  1:5481342/101‑1 (MQ=21)
                              ggCACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                           >  1:1639647/1‑101 (MQ=21)
                               gcatggcAGGCC.GAGTACG.GGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                          <  2:5662156/95‑1 (MQ=17)
                                cACGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                         <  2:6470756/101‑1 (MQ=21)
                                  cGACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                       <  1:1883130/101‑1 (MQ=21)
                                   gACAGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                      >  1:967734/1‑101 (MQ=21)
                                      aGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                   <  1:2055620/101‑1 (MQ=21)
                                      aGGCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                   >  1:316536/1‑101 (MQ=21)
                                       ggCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                  <  2:2710869/101‑1 (MQ=21)
                                       ggCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                  <  2:3722321/101‑1 (MQ=21)
                                        gCC.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                 <  1:4924078/101‑1 (MQ=21)
                                         cc.GATTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                <  2:40144/101‑1 (MQ=18)
                                         cc.GAGTA.A.GGGGTGAGCGCTG.CCCCCCGTCCGCAAGCGTACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                <  2:2836554/101‑1 (MQ=21)
                                          c.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                               >  1:1753761/1‑101 (MQ=18)
                                          c.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                               <  2:1032815/101‑1 (MQ=18)
                                          c.GAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                               <  2:4457073/101‑1 (MQ=21)
                                            gAGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                              <  1:6109914/101‑1 (MQ=18)
                                             aGTA.AGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                             <  1:1425872/101‑1 (MQ=18)
                                               gtaaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                            >  2:5005216/6‑101 (MQ=255)
                                               gtaaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                            >  1:5311442/6‑101 (MQ=255)
                                                taaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATCTTTGCAGCAAGATGAAACCGGATGCCCTTCATCTTGCCCTAAATATCCCggg                                                           >  1:2370275/5‑101 (MQ=18)
                                                gaaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                           <  2:45779/97‑1 (MQ=255)
                                                  aggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                         >  2:202817/3‑101 (MQ=255)
                                                   ggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                        >  2:5504117/2‑101 (MQ=255)
                                                   ggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                        >  2:1828193/2‑101 (MQ=255)
                                                     ggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggggg                                                      >  1:3456816/1‑99 (MQ=255)
                                                     ggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggggg                                                      >  2:6196447/1‑99 (MQ=255)
                                                      gg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                     <  1:914235/101‑1 (MQ=255)
                                                      gg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                     >  2:4706240/1‑101 (MQ=255)
                                                      gg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                     >  1:6687036/1‑101 (MQ=255)
                                                         gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                  >  1:503492/1‑101 (MQ=255)
                                                           gcgcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                <  2:6960075/101‑1 (MQ=255)
                                                           gcgcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                <  1:6946460/101‑1 (MQ=255)
                                                            cgcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                               >  2:1581032/1‑101 (MQ=255)
                                                            cgcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                               <  1:3334951/101‑1 (MQ=255)
                                                             gcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                            <  2:2157931/101‑1 (MQ=255)
                                                               ctccccccCGTCCGCAAGCCGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                          <  1:848607/98‑1 (MQ=18)
                                                                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGAAAGATGAAACCGGATGCCTTTCATCTTGCCCGAGATATCCCGGGGGGTGAA.TGGC..ggacgca                                      >  2:1215755/1‑95 (MQ=18)
                                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                    <  2:6425092/97‑7 (MQ=255)
                                                                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                   <  2:80028/98‑8 (MQ=255)
                                                                              aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCa                            >  2:3446682/1‑101 (MQ=18)
                                                                              aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCa                            <  1:4841224/101‑1 (MQ=18)
                                                                                gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagc                          <  1:3524300/101‑3 (MQ=18)
                                                                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                         >  2:3608282/1‑98 (MQ=18)
                                                                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                         >  1:381771/1‑98 (MQ=18)
                                                                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                         >  2:4103598/1‑98 (MQ=18)
                                                                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                        >  2:7107447/1‑97 (MQ=18)
                                                                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAACATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                        >  1:6144098/1‑97 (MQ=18)
                                                                                   gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cc                     >  2:30725/1‑101 (MQ=18)
                                                                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCaccgcccc                     >  2:5968552/1‑94 (MQ=18)
                                                                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccc                 >  1:222273/1‑100 (MQ=17)
                                                                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGG.AGGCG.cccccc                 >  2:2902251/1‑100 (MQ=17)
                                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                <  2:4196590/101‑3 (MQ=17)
                                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccca                <  2:5732257/101‑3 (MQ=17)
                                                                                          cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccctgc              <  2:4881664/101‑5 (MQ=17)
                                                                                            cGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCAGCGG.CCCCCTGccc             >  2:3513234/1‑101 (MQ=18)
                                                                                             gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGcccc            >  1:6228942/1‑101 (MQ=0)
                                                                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGcccc            >  1:6612003/1‑101 (MQ=17)
                                                                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGcccc            <  2:1733916/101‑1 (MQ=17)
                                                                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcg           >  1:2857575/1‑101 (MQ=2)
                                                                                              ggATATTTGCAGCAAGATGAAACAGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccctgccacg          >  1:3366503/1‑93 (MQ=14)
                                                                                               gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg          >  2:4306352/1‑101 (MQ=18)
                                                                                                 tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccg        >  1:2739692/1‑101 (MQ=2)
                                                                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg        >  2:2512571/1‑101 (MQ=17)
                                                                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcgccg        >  2:3164905/1‑101 (MQ=17)
                                                                                                  atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGCGGGCAGCCC.CCCCCTGCCCcggcgg       >  2:6129407/1‑101 (MQ=18)
                                                                                                   tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcggc      >  1:1611925/1‑100 (MQ=17)
                                                                                                    ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTc     >  1:3639477/1‑101 (MQ=17)
                                                                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc    <  1:707982/101‑1 (MQ=17)
                                                                                                       cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AAGCCAGGGGGGGGCAGCGCCCCCCTGCCCCGGCGGTCCGt  <  1:1021714/101‑1 (MQ=18)
                                                                                                        |                                                                                                       
GCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTA.A.GGGG.GGGCGCTG.CCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGT  >  NC_014034/2493904‑2494101

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG