Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 4.1% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 4.1% 19.3 ‑0.3 ‑0.0290intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (121/157):  total (127/163)
Fisher's exact test strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.08e-01

Only 100 of 318 total aligned reads displayed.

CCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTAA.GGGG.GGGCGCTG.CCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117
                                                                                                       |                                                                                                        
ccgcGGCGGGCGCGCGGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCgg                                                                                                          <  1:1632771/101‑1 (MQ=21)
  gcggcgGGCGCGCGGCACGACAGGCCGGGGAAA.GGGG.GGGGGCTG.CCCCCCGTCCGGCAGCGGGCTCCCCCCCGGCAATTTGCCGCACAAAGAAACCGGaa                                                                                                        >  1:1170684/1‑100 (MQ=255)
     gcgggcgCGCGGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                     <  2:1668214/101‑1 (MQ=21)
     gccggcgCGCGGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                     <  1:6940405/98‑1 (MQ=18)
      cgggcgCGCGGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                    <  1:3704793/101‑1 (MQ=21)
       gggcgCGCGGCACGACAGGCC.GAGTAAGGGGG.GGGGGCCGCCCCCCGGCCCGCAAGCGGCCTCCCCCCGGGAAATTTGCGCAAAGAGAAAACCGGAGGCCt                                                                                                    >  2:5345353/1‑101 (MQ=255)
       gggccCGCGGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                   <  2:6566675/101‑1 (MQ=18)
        ggcgCGCGGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                  >  1:5868744/1‑101 (MQ=21)
         gcgcgcGGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                 <  2:1128414/101‑1 (MQ=21)
           gcgcgGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                               <  1:4183156/101‑1 (MQ=21)
           gcgcgGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                               <  1:5963658/101‑1 (MQ=21)
            cgcgGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                              <  1:4354939/101‑1 (MQ=21)
             gcgGCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                             <  1:458653/101‑1 (MQ=21)
              cgGCAGGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                            <  1:1844552/101‑1 (MQ=18)
               ggCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                           >  1:5687611/1‑101 (MQ=21)
               ggCACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                           <  2:6596797/101‑1 (MQ=21)
                 cACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                         <  1:884545/101‑1 (MQ=21)
                 cACGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                         <  2:6470756/101‑1 (MQ=21)
                  aCGACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGGCCGCCAGCGGAATCCCCCCGGGGTATTTGCAGCAAAAAGAAAACGGATGCCTTTCATCTTGgcc                                                                                        >  1:3499991/1‑101 (MQ=18)
                    gACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGGCCGa                                                                                      >  1:3212653/1‑101 (MQ=18)
                    gACAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                      >  1:967734/1‑101 (MQ=21)
                      gaGGTC.GAGTAAGGGGG.GGGCGCCG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                    <  2:4485748/100‑1 (MQ=18)
                      cAGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                    <  2:6934405/101‑1 (MQ=21)
                       aGGCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                   <  1:2055620/101‑1 (MQ=21)
                        ggCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                  <  2:2710869/101‑1 (MQ=21)
                        ggCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                  >  1:6581394/1‑101 (MQ=21)
                        ggCC.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                  <  2:3722321/101‑1 (MQ=21)
                          cc.GAGTAA.GGGGTGAGCGCTG.CCCCCCGTCCGCAAGCGTACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                <  2:2836554/101‑1 (MQ=21)
                           c.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                               <  2:1032815/101‑1 (MQ=18)
                           c.GAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                               <  1:7103377/101‑1 (MQ=21)
                            cgactaaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatatac                                                                               >  1:120244/9‑99 (MQ=18)
                             gAGTAAGGGGG.GGGCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                              <  1:6109914/101‑1 (MQ=18)
                               gtaaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                            >  2:5005216/6‑101 (MQ=255)
                                taaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                           >  2:2995194/5‑101 (MQ=255)
                                 aaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                          <  1:2951550/98‑1 (MQ=255)
                                 aaggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                          >  2:707962/4‑101 (MQ=255)
                                  aggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                         >  2:202817/3‑101 (MQ=255)
                                  aggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                         >  1:1594922/3‑101 (MQ=255)
                                  aggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCggggg                                                                         >  1:1715591/3‑101 (MQ=255)
                                   ggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        >  2:5504117/2‑101 (MQ=255)
                                   ggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        >  2:1828193/2‑101 (MQ=255)
                                   ggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                        >  1:3122284/2‑101 (MQ=255)
                                   ggggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATACCCCgggggg                                                                        <  1:6828304/100‑1 (MQ=255)
                                    gggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                       >  2:4799099/1‑100 (MQ=255)
                                     ggg.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                      <  1:1978697/101‑1 (MQ=255)
                                       g.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                    <  1:6962873/101‑1 (MQ=255)
                                       g.gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGAAGCAAGATGACACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGaa                                                                    >  2:4653910/1‑101 (MQ=17)
                                         gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                  <  1:6616493/101‑1 (MQ=255)
                                         gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                  <  1:4792651/101‑1 (MQ=255)
                                         gggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                  >  1:503492/1‑101 (MQ=255)
                                          ggCGCTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                 <  1:4921060/101‑1 (MQ=255)
                                           gcgcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTCAA.Tgg                                                                <  2:3512697/101‑1 (MQ=255)
                                             gcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                            >  2:5881401/1‑101 (MQ=255)
                                             gcTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                            <  2:2157931/101‑1 (MQ=255)
                                              cTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                          <  2:4569864/101‑1 (MQ=255)
                                              cTG.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.a                                                          >  1:5416968/1‑101 (MQ=255)
                                                g.CCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                        >  1:4097084/1‑99 (MQ=255)
                                                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                     <  1:6068986/101‑6 (MQ=255)
                                                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGAAAGATGAAACCGGATGCCTTTCATCTTGCCCGAGATATCCCGGGGGGTGAA.TGGC..ggacgca                                                      >  2:1215755/1‑95 (MQ=18)
                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                    <  1:379658/97‑7 (MQ=255)
                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                    <  2:6425092/97‑7 (MQ=255)
                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                    <  1:2613364/97‑7 (MQ=255)
                                                      ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                   <  1:5334996/98‑8 (MQ=255)
                                                        gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                 >  1:3840489/2‑92 (MQ=255)
                                                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                         >  2:4103598/1‑98 (MQ=18)
                                                                 cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcg                                         >  2:3608282/1‑98 (MQ=18)
                                                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAACATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGCagcgc                                        >  1:6144098/1‑97 (MQ=18)
                                                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccc                                   >  2:4049367/1‑101 (MQ=18)
                                                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccc                                   <  2:718542/101‑1 (MQ=17)
                                                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccc                                 >  1:2407036/1‑100 (MQ=17)
                                                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccc                                 >  1:4038/1‑100 (MQ=17)
                                                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccc                                 <  2:1570929/101‑2 (MQ=17)
                                                                       ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGG.AGGCG.cccccc                                 >  2:2902251/1‑100 (MQ=17)
                                                                        cccccGTGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                                <  1:3292237/101‑3 (MQ=17)
                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                                <  2:3212537/101‑3 (MQ=17)
                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                                <  2:3550655/101‑3 (MQ=17)
                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccct                                <  2:4196590/101‑3 (MQ=17)
                                                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCG.cccccca                                <  2:5732257/101‑3 (MQ=17)
                                                                             gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGcccc                            <  2:6159028/101‑1 (MQ=0)
                                                                             gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AGGCCAAGAGGGGGCAGCG.CCCCCTGcccc                            >  1:6228942/1‑101 (MQ=0)
                                                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGcccc                            <  2:1733916/101‑1 (MQ=17)
                                                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcg                           <  2:5653150/101‑1 (MQ=17)
                                                                               gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcgg                          >  2:4306352/1‑101 (MQ=18)
                                                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCcggcg                        >  2:2512571/1‑101 (MQ=17)
                                                                                   tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGt                      >  2:5128325/1‑101 (MQ=17)
                                                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                    >  2:6090311/1‑101 (MQ=18)
                                                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                    >  1:3578887/1‑101 (MQ=18)
                                                                                       cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AAGCCAGGGGGGGGCAGCGCCCCCCTGCCCCGGCGGTCCGt                  <  1:1021714/101‑1 (MQ=18)
                                                                                          caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc               >  2:3891263/1‑101 (MQ=18)
                                                                                           aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGCCCGTggcg              >  1:3383889/1‑101 (MQ=17)
                                                                                            aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGGGGTCCGGgccgg             >  1:2858290/1‑101 (MQ=9)
                                                                                             tggaaaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcggc            >  2:1944999/5‑101 (MQ=17)
                                                                                               tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGgcgc          >  2:3155880/1‑101 (MQ=18)
                                                                                                gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc         >  1:334723/1‑101 (MQ=18)
                                                                                                  aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt       >  1:2830502/1‑101 (MQ=18)
                                                                                                   aCCGGATGCCTTTCATCTTGCCCGAAATATGCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      <  1:229743/101‑1 (MQ=17)
                                                                                                   aCCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      <  2:1348192/101‑1 (MQ=17)
                                                                                                    ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa     >  2:4431943/1‑101 (MQ=18)
                                                                                                     cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGCGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    <  2:3456692/101‑1 (MQ=17)
                                                                                                      ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCGTGAcg   >  1:3440069/1‑101 (MQ=17)
                                                                                                       |                                                                                                        
CCGCGGCGGGCGCGCGGCACGACAGGCC.GAGTAA.GGGG.GGGCGCTG.CCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AGCAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493919‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG