Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,048 G→A 4.5% intergenic (+96/‑146) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0480G→A 4.5% 22.1 0.0 ‑0.0264intergenic (+96/‑146)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (132/120):  total (138/126)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.65e-01

Only 100 of 305 total aligned reads displayed.

AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGC.G.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCA  >  NC_014034/2493948‑2494147
                                                                                                    |                                                                                                           
gtaaggggggggCGCTGCCACCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                             <  1:2002858/96‑1 (MQ=18)
 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATCTTTGCAGCAAGATGAAACCGGATGCCCTTCATCTTGCCCTAAATATCCCggg                                                                                                            >  1:2370275/5‑101 (MQ=18)
 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                            >  1:6893672/5‑101 (MQ=255)
 gaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                            <  2:45779/97‑1 (MQ=255)
  aagcggggggCGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                           <  2:5713439/97‑1 (MQ=18)
   aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                                          >  1:1594922/3‑101 (MQ=255)
     gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                                                        <  2:2918559/101‑1 (MQ=255)
      ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                       <  1:6585162/101‑1 (MQ=255)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                      <  1:914235/101‑1 (MQ=255)
        ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                                                     >  2:1473154/1‑101 (MQ=255)
        ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                                                     <  1:6962873/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCGCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   <  1:3404206/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   <  1:2840836/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   <  1:6616493/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   <  1:4792651/101‑1 (MQ=255)
          ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                  >  2:3557005/1‑101 (MQ=255)
          ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                  <  1:4921060/101‑1 (MQ=255)
           gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                                                 <  2:6960075/101‑1 (MQ=255)
            cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                <  2:1639586/101‑1 (MQ=255)
             gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                             >  2:5334729/1‑101 (MQ=255)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                         >  1:4097084/1‑99 (MQ=255)
                  ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                      <  1:1689703/101‑6 (MQ=255)
                     ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                    <  1:2421694/98‑8 (MQ=255)
                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                                                            >  2:3446682/1‑101 (MQ=18)
                               gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                                                          >  1:5933131/1‑99 (MQ=18)
                                cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                                                         >  1:381771/1‑98 (MQ=18)
                                 ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                                                        >  2:7107447/1‑97 (MQ=18)
                                  gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.cc                                                                    >  1:2701939/1‑101 (MQ=18)
                                   aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.cc.c                                                                  >  2:6585192/1‑101 (MQ=18)
                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGC.G.cc.ccc                                                                <  2:2668826/101‑1 (MQ=17)
                                      ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.cc.cccc                                                               >  1:4038/1‑100 (MQ=17)
                                      ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.cc.cccc                                                               <  2:120243/101‑2 (MQ=17)
                                        ccccGGGATATTGGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.cc.cccctg                                                             >  1:6308386/1‑98 (MQ=17)
                                         cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.cc.cccctgc                                                            <  2:4881664/101‑5 (MQ=17)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGcccc                                                          <  2:1733916/101‑1 (MQ=17)
                                             ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGC.G.CC.CCCTGCCCcg                                                         <  2:6340765/101‑1 (MQ=0)
                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGC.G.CC.CCCTGCCCcgcc                                                       <  1:6014660/101‑3 (MQ=2)
                                               atatTTGCAGCAAGATGAACCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCcggc                                                       <  1:6198708/101‑1 (MQ=17)
                                                tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGC.G.CC.CCCTGCCCcgccg                                                      >  2:4669774/1‑101 (MQ=2)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCcggcg                                                      >  1:6142998/1‑101 (MQ=17)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCcggcg                                                      >  2:2512571/1‑101 (MQ=17)
                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGCGGGCAGCC.C.CC.CCCTGCCCcggcgg                                                     >  2:6129407/1‑101 (MQ=18)
                                                  tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCcggcggg                                                    >  1:3783275/1‑100 (MQ=17)
                                                   ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGC.G.CC.CCCTGCCCcgccggat                                                   <  2:6476340/101‑3 (MQ=2)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTcc                                                  <  1:6766555/101‑1 (MQ=17)
                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCg                                                 <  1:315144/101‑1 (MQ=17)
                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTggc                                             >  2:3891263/1‑101 (MQ=18)
                                                            tggaaaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTggcggc                                          >  2:1944999/5‑101 (MQ=17)
                                                              tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCACGGCGGTCCGTAGCGgcgc                                        >  2:1810558/1‑101 (MQ=17)
                                                              tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCAGTGGCGgcgc                                        >  2:605370/1‑101 (MQ=17)
                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGcc                                       >  1:3054694/1‑101 (MQ=17)
                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGcc                                       >  1:334723/1‑101 (MQ=18)
                                                               gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGcc                                       >  1:2865893/1‑101 (MQ=17)
                                                                aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                      >  2:2019594/1‑101 (MQ=18)
                                                                 aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                     <  2:5769379/101‑1 (MQ=17)
                                                                 aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                     <  2:4070976/101‑1 (MQ=17)
                                                                  aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                    <  2:375861/101‑1 (MQ=17)
                                                                   ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                   >  2:4431943/1‑101 (MQ=18)
                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                  <  2:3499855/101‑1 (MQ=17)
                                                                     ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                 >  2:4260213/1‑101 (MQ=17)
                                                                     ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                 >  2:2494285/1‑101 (MQ=18)
                                                                      gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                <  2:3541273/101‑1 (MQ=17)
                                                                      gATGCCTTTCATCTTGCCCGAAATAGCCGGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                <  2:5558272/101‑1 (MQ=17)
                                                                         gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                             >  2:4196347/1‑101 (MQ=17)
                                                                          ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                            >  1:4061165/1‑101 (MQ=18)
                                                                           cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                           >  2:3644775/1‑101 (MQ=18)
                                                                           cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAccggcggg                           >  2:412531/1‑101 (MQ=17)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCT.C.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                         <  1:1009584/101‑1 (MQ=18)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCCGGGCggggg                         >  2:109252/1‑101 (MQ=17)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                         >  1:740644/1‑101 (MQ=18)
                                                                              tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCGCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                        >  1:5355279/1‑100 (MQ=17)
                                                                              tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                        <  1:1717802/101‑1 (MQ=18)
                                                                               cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.CCGCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                       >  1:6434785/1‑101 (MQ=18)
                                                                                 tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                     <  2:6181575/101‑1 (MQ=18)
                                                                                 cttggCCCGAAATTACCCGGGGGGGGGA.AGGCG.G.AG.CCCAGGGGGGGGCAGC.C.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                     <  1:3611082/97‑1 (MQ=255)
                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                    >  2:2057294/1‑101 (MQ=18)
                                                                                  cTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAAAGGGGGG.AAGCAC.CC.CCCTGCCCCAGTGGTCCGTGGCGGCGCCTTGCCTGGCGGGGGCGGgg                    >  2:2397589/1‑101 (MQ=16)
                                                                                   ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                   <  2:316532/101‑1 (MQ=18)
                                                                                   ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                   <  1:3914345/101‑1 (MQ=18)
                                                                                    tgaccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.G.CCACCCTGCCCCGGAGGAAAGTGAAGGCGCCTTGTCGGGCGGGGGGGCGGGc                  >  1:3451881/4‑101 (MQ=2)
                                                                                    tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                  >  2:1837980/1‑101 (MQ=18)
                                                                                     gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCA.C.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                 >  1:5227265/1‑101 (MQ=21)
                                                                                      cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                >  2:2824829/1‑101 (MQ=18)
                                                                                      cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                <  1:4137702/101‑1 (MQ=18)
                                                                                      cccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                <  2:5242518/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt               <  1:3557141/101‑1 (MQ=18)
                                                                                       ccGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CA.AGGGGGGGGCAGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt               <  1:1136679/101‑1 (MQ=21)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc            <  2:2995275/101‑1 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc            <  2:3122171/101‑1 (MQ=18)
                                                                                          aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGCGCGGGGGCGCCGGCATTAAc            >  1:4250618/1‑101 (MQ=18)
                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc           >  1:1912982/1‑101 (MQ=21)
                                                                                           aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc           >  1:609426/1‑101 (MQ=18)
                                                                                             tatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa         >  2:4738294/1‑101 (MQ=21)
                                                                                              atCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGaa        >  1:4643341/1‑101 (MQ=21)
                                                                                               tCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa       <  1:5776040/101‑1 (MQ=21)
                                                                                                  cGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGCAATTg    >  2:5065239/1‑101 (MQ=21)
                                                                                                   ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc   >  1:3245133/1‑101 (MQ=25)
                                                                                                   ggggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc   >  1:1473541/1‑101 (MQ=25)
                                                                                                    gggggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGC.GCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa  >  2:5922178/1‑101 (MQ=25)
                                                                                                    gggggTGAA.GGGC..GAAG.CCAGAGGGGGGCAGCC.C.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa  <  1:2273409/101‑1 (MQ=25)
                                                                                                    |                                                                                                           
AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGC.G.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCA  >  NC_014034/2493948‑2494147

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG