Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,549,153 G→A 100% G56S (GGC→AGC)  tonB → protein TonB

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,549,1530G→A100.0% 964.3 252G56S (GGC→AGC) tonBprotein TonB
Reads supporting (aligned to +/- strand):  new base (114/138):  ref base (0/0):  total (114/138)

Only 100 of 267 total aligned reads displayed.

CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549055‑2549250
                                                                                                  |                                                                                                 
cGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGc                                                                                                 <  1:7068104/101‑1 (MQ=255)
 gCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGCGGGCCTGGCCAGcc                                                                                                >  1:872867/1‑101 (MQ=255)
  cTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCt                                                                                               >  2:2401769/1‑101 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGGCTc                                                                                              >  1:6457587/1‑101 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTc                                                                                              <  1:5279567/101‑1 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTc                                                                                              >  1:4562139/1‑101 (MQ=255)
    ggCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCg                                                                                             <  1:6269010/101‑1 (MQ=255)
     gcgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                            <  1:4726476/101‑1 (MQ=255)
     gcgcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCCGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGa                                                                                            <  2:3451380/101‑1 (MQ=255)
        cTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGc                                                                                         <  1:102954/101‑1 (MQ=255)
        cTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGc                                                                                         <  2:6029815/101‑1 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCg                                                                                        >  2:48395/1‑101 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCg                                                                                        <  1:2959920/101‑1 (MQ=255)
           cATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGAt                                                                                      >  2:578433/1‑101 (MQ=255)
            gtGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     <  2:924148/100‑1 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     <  2:1569429/101‑1 (MQ=255)
             tGCGGTCGCGGTTTCCGGGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcg                                                                                    <  1:1532979/101‑1 (MQ=255)
              gCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGCCGGCGAGGATCTGGACAGCCTCGAAGCGATcgc                                                                                   <  1:5010784/101‑1 (MQ=255)
               cGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTCTGGACAGCCTCGAAGCGATcgcc                                                                                  >  2:6204410/1‑101 (MQ=255)
               cGGTCGCGGTGTCAGTGCTCTCGCAGCCCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgcc                                                                                  <  2:2487013/101‑1 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                <  1:3147716/101‑1 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                <  1:1430087/101‑1 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                >  2:3136153/1‑101 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                >  2:5153974/1‑101 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                <  2:5045808/101‑1 (MQ=255)
                  tCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCcgcg                                                                               <  1:5473269/101‑1 (MQ=255)
                  tCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCcgcg                                                                               >  2:3560758/1‑101 (MQ=255)
                     cgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGAcc                                                                            <  1:1585273/101‑1 (MQ=255)
                      ggTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCt                                                                           >  2:4037558/1‑101 (MQ=255)
                       gTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCtt                                                                          >  1:2796308/1‑101 (MQ=255)
                       gTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCtt                                                                          <  1:2084183/101‑1 (MQ=255)
                       gTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCtt                                                                          >  1:4995600/1‑101 (MQ=255)
                        tttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtc                                                                         <  2:4742502/101‑1 (MQ=255)
                          tCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgt                                                                       >  2:6544063/1‑101 (MQ=255)
                           ccGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtc                                                                      >  2:4431464/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     >  2:5436735/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     >  1:990499/1‑101 (MQ=255)
                             gTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGa                                                                    <  1:6556125/101‑1 (MQ=255)
                                ctctCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCt                                                                 >  1:3327757/1‑101 (MQ=255)
                                   tcGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGACGCGACCTTCGTCGATCTGgc                                                              >  2:1799602/1‑101 (MQ=255)
                                    cGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgcg                                                             <  2:5539228/101‑1 (MQ=255)
                                     gCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgcgc                                                            <  2:460740/101‑1 (MQ=255)
                                     gCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgcgc                                                            >  2:6668737/1‑101 (MQ=255)
                                     gCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgcgc                                                            <  1:1047704/101‑1 (MQ=255)
                                      cAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgcc                                                           >  2:3986665/1‑101 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          >  2:4700506/1‑101 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          >  1:2532961/1‑101 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          >  1:320053/1‑101 (MQ=255)
                                        gACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccgc                                                         <  1:1757077/101‑1 (MQ=255)
                                          ccGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCCACCTTCGTCGATCTGGCGCCGCAg                                                       >  2:4160835/1‑101 (MQ=255)
                                            gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  2:3628984/1‑101 (MQ=255)
                                            gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  1:5945502/1‑101 (MQ=255)
                                            gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  2:2968324/1‑101 (MQ=255)
                                             aGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATc                                                    <  2:6485672/101‑1 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGGGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCCACGATCCGGCGCCGCAGATcc                                                   >  1:5624843/1‑101 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                   <  1:2401863/101‑1 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                   <  2:4546070/101‑1 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATcc                                                   <  1:4655540/101‑1 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCGTCGTCGATCTGGCGCCGCAGATcc                                                   <  2:3407598/101‑1 (MQ=255)
                                              ggCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGAACTTCGTCGATCTGGCGCCGCAGATcc                                                   >  2:1626703/1‑101 (MQ=255)
                                                  gccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGGGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGGGCGGCAGATccgccg                                               >  2:6941287/1‑97 (MQ=255)
                                                  gccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCt                                               <  1:3480809/101‑1 (MQ=255)
                                                  gccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCt                                               >  1:7015361/1‑101 (MQ=255)
                                                    ccccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgc                                             <  1:48396/101‑1 (MQ=255)
                                                     cccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcc                                            <  1:1650619/101‑1 (MQ=255)
                                                        gcggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccga                                         <  2:347016/101‑1 (MQ=255)
                                                          ggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagc                                       <  1:6815644/101‑1 (MQ=255)
                                                          ggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagc                                       >  1:3428682/1‑101 (MQ=255)
                                                              cTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccga                                   <  1:1848747/101‑1 (MQ=255)
                                                              cTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccga                                   <  1:6678864/101‑1 (MQ=255)
                                                                ggtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCgaga                                 <  2:7042343/101‑1 (MQ=255)
                                                                 gtggGGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGAt                                <  2:3819108/101‑1 (MQ=255)
                                                                 gtggCGGCTGGCGCGGGCGGGGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGAt                                <  1:909638/101‑1 (MQ=255)
                                                                 gtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGAt                                <  1:1500201/101‑1 (MQ=255)
                                                                  gggcggcGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATg                               <  2:1963635/100‑1 (MQ=255)
                                                                    gcggcgGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCGGCTGCCCGAGCCCGAGATGgc                             <  2:6779088/101‑1 (MQ=255)
                                                                    gcggcgGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGgc                             <  1:3584170/101‑1 (MQ=255)
                                                                        cgggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTg                         <  2:2901500/101‑1 (MQ=255)
                                                                              cgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTg                   >  2:1453550/1‑101 (MQ=255)
                                                                              cgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTg                   >  1:4228795/1‑101 (MQ=255)
                                                                               gggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGa                  >  1:1460908/1‑101 (MQ=255)
                                                                               gggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGa                  >  1:5869307/1‑101 (MQ=255)
                                                                                ggcggcGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                 <  1:3991386/101‑1 (MQ=255)
                                                                                 gcggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAcc                <  2:295728/101‑1 (MQ=255)
                                                                                 gcggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAcc                <  2:1939818/101‑1 (MQ=255)
                                                                                 gcggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAcc                <  1:3744817/101‑1 (MQ=255)
                                                                                 gcggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCCGCCCGAGCCCGAGATGGCGCTGCCGCTGAcc                <  1:5722899/101‑1 (MQ=255)
                                                                                  cggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcg               <  1:1216254/101‑1 (MQ=255)
                                                                                   ggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcgc              >  2:526766/1‑101 (MQ=255)
                                                                                     ggAGGATCTGGACAGCCGCGAAGCGATCGCCGCGGCCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCgcgc            <  2:636967/100‑1 (MQ=255)
                                                                                      gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGcc           <  2:1102384/101‑1 (MQ=255)
                                                                                      gAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATAGCGCTGCCGCTGACCGCGcc           >  1:4375975/1‑101 (MQ=255)
                                                                                        ggATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCgg         >  2:1338749/1‑101 (MQ=255)
                                                                                        ggATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCgg         >  2:2304020/1‑101 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGt        >  1:4395997/1‑101 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGt        >  2:727069/1‑101 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGt        >  2:686403/1‑101 (MQ=255)
                                                                                            cTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGAt     >  1:2105198/1‑101 (MQ=255)
                                                                                            cTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGAt     >  2:6606341/1‑101 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   >  1:1728320/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549055‑2549250

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG