Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,554,356 T→C 100% N96D (AAC→GAC)  mraW ← S‑adenosyl‑methyltransferase MraW

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,554,3560T→C100.0% 1186.3 315N96D (AAC→GAC) mraWS‑adenosyl‑methyltransferase MraW
Reads supporting (aligned to +/- strand):  new base (142/173):  ref base (0/0):  total (142/173)

Only 100 of 325 total aligned reads displayed.

CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453
                                                                                                  |                                                                                                 
cATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCGCCCCGTCCa                                                                                                 <  1:3482545/101‑1 (MQ=255)
    cGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGccc                                                                                             <  1:5767684/101‑1 (MQ=255)
     gCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCt                                                                                            <  1:1003039/101‑1 (MQ=255)
     gCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCt                                                                                            >  2:4078970/1‑101 (MQ=255)
      cATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCtt                                                                                           >  2:3878260/1‑101 (MQ=255)
       ttGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTc                                                                                          <  2:1689267/100‑1 (MQ=255)
       aTGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTc                                                                                          >  1:3182978/1‑101 (MQ=255)
       aTGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTc                                                                                          <  2:6531748/101‑1 (MQ=255)
          tCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCggg                                                                                       <  2:3257561/101‑1 (MQ=255)
            cAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcg                                                                                     >  1:3613278/1‑101 (MQ=255)
            cAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcg                                                                                     >  1:3535495/1‑101 (MQ=255)
             aaGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcgc                                                                                    >  2:5937670/1‑101 (MQ=255)
               gggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGa                                                                                  >  2:5208111/1‑101 (MQ=255)
                ggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGac                                                                                 >  2:5275694/1‑100 (MQ=255)
                ggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAt                                                                                 >  2:2706125/1‑101 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              >  1:281133/1‑101 (MQ=255)
                    cGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcg                                                                             >  1:4171812/1‑101 (MQ=255)
                     gTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcgg                                                                            >  1:6999049/1‑101 (MQ=255)
                      tCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggc                                                                           >  1:2568355/1‑101 (MQ=255)
                        ggaaaaaaaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcgg                                                                         <  2:4210477/95‑1 (MQ=255)
                        gcgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcgg                                                                         >  2:2825889/1‑101 (MQ=255)
                        gcgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcgg                                                                         >  2:1385427/1‑101 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       <  2:2103238/101‑1 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       >  2:3636181/1‑101 (MQ=255)
                           cAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGa                                                                      >  1:6723029/1‑101 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    <  2:1897970/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    <  1:4615491/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    >  2:762694/1‑101 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               >  2:6668901/1‑101 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  1:3942982/101‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  1:1188236/101‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               >  1:4998201/1‑101 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  2:4917129/101‑1 (MQ=255)
                                   aaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              <  2:1617279/101‑1 (MQ=255)
                                   aaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              >  2:3822863/1‑101 (MQ=255)
                                   aaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              <  1:612399/101‑1 (MQ=255)
                                   aaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              >  1:1824641/1‑101 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             <  1:3252989/101‑1 (MQ=255)
                                     aaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAg                                                            <  1:5514301/101‑1 (MQ=255)
                                     aaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAg                                                            <  2:5529822/101‑1 (MQ=255)
                                      aGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAgg                                                           <  1:427113/101‑1 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         <  1:2190780/101‑1 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         >  1:2023796/1‑101 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         >  1:4473445/1‑101 (MQ=255)
                                         cgcgCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTc                                                        <  1:1492492/101‑1 (MQ=255)
                                          gcgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCg                                                       <  1:6000275/101‑1 (MQ=255)
                                           cgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGa                                                      <  1:4757172/101‑1 (MQ=255)
                                             cTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCTAGGTTCGaaa                                                    >  2:6893597/1‑101 (MQ=255)
                                             cTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaa                                                    >  2:221368/1‑101 (MQ=255)
                                              tCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaaa                                                   <  2:6789029/101‑1 (MQ=255)
                                               cGGCCTGATCGAGCTGCATCCACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCCTCGAGGTTCCAAAAg                                                  <  1:5635482/101‑1 (MQ=255)
                                                ggCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAgg                                                 <  1:535503/101‑1 (MQ=255)
                                                 gCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAggg                                                >  2:3937351/1‑100 (MQ=255)
                                                  ccTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgtg                                               >  1:2232573/1‑101 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             >  2:3566691/1‑101 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             >  1:332833/1‑101 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGGGtt                                             >  1:984835/1‑101 (MQ=255)
                                                      gtCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtct                                           <  1:589958/100‑1 (MQ=255)
                                                      aTCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtct                                           <  2:6722687/101‑1 (MQ=255)
                                                       tCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtctc                                          >  2:2874242/1‑101 (MQ=255)
                                                       tCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtctc                                          <  2:1334475/101‑1 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         <  2:84690/101‑1 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         >  1:1654195/1‑101 (MQ=255)
                                                        cGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCg                                         <  1:3226726/101‑1 (MQ=255)
                                                          aGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGac                                       <  2:5396677/101‑1 (MQ=255)
                                                           gCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGacc                                      <  2:1207105/101‑1 (MQ=255)
                                                            cTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGGCGGCATCGAGGTTCGAAAAGGGGTTCTCGcccc                                     >  1:5277888/1‑100 (MQ=255)
                                                            cTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGacca                                     <  1:2763333/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   >  1:6809028/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   <  2:4371956/101‑1 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 <  1:1252321/101‑1 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 >  2:5516597/1‑101 (MQ=255)
                                                                 tCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacc                                <  1:866629/101‑1 (MQ=255)
                                                                 tCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacc                                <  2:6181750/101‑1 (MQ=255)
                                                                 tCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacc                                <  1:6515036/101‑1 (MQ=255)
                                                                  cgacgaCACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacca                               <  2:6071750/101‑1 (MQ=255)
                                                                   gacgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccag                              >  2:2533949/1‑101 (MQ=255)
                                                                   gacgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGCTCGAAAAGGTGTTCTCGaccagcacaac                              >  2:4656770/1‑98 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  2:5002441/101‑1 (MQ=255)
                                                                       acacGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGc                          >  1:6871944/1‑101 (MQ=255)
                                                                       acacGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGc                          <  2:413354/101‑1 (MQ=255)
                                                                         acGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTc                        >  2:625507/1‑101 (MQ=255)
                                                                         acGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTc                        <  1:5221518/101‑1 (MQ=255)
                                                                          cGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCg                       <  2:5503887/101‑1 (MQ=255)
                                                                              cAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGa                   <  2:4244890/101‑1 (MQ=255)
                                                                               aGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGac                  >  2:4562957/1‑100 (MQ=255)
                                                                               aGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGaa                  <  1:585448/101‑1 (MQ=255)
                                                                                gATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAAc                 >  2:5632821/1‑101 (MQ=255)
                                                                                gATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAAc                 >  1:1695961/1‑101 (MQ=255)
                                                                                 aTCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                <  1:5142814/101‑1 (MQ=255)
                                                                                 aTCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                <  1:3011485/101‑1 (MQ=255)
                                                                                  tCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTc               <  1:6743995/101‑1 (MQ=255)
                                                                                  tCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTc               <  2:332829/101‑1 (MQ=255)
                                                                                    cAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgc             <  1:3873016/101‑1 (MQ=255)
                                                                                       caccacCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgccgc          >  2:3377794/1‑101 (MQ=255)
                                                                                        accaccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgccgcc         >  1:624010/1‑100 (MQ=255)
                                                                                        accaccCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCcgcg         >  1:7115316/1‑101 (MQ=255)
                                                                                             cccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAgg    >  1:2838904/1‑101 (MQ=255)
                                                                                               cGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGGCGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCCGGgc  >  1:2430938/1‑101 (MQ=255)
                                                                                               cGTCCAGCCCTTCGGGCGCGATCGCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGgc  <  2:6998675/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG