Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,559,923 G→C 100% intergenic (‑199/+71) ispZ ← / ← ftsY intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,559,9230G→C100.0% 727.0 207intergenic (‑199/+71)ispZ/ftsYintracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand):  new base (150/56):  ref base (0/0):  total (151/56)

Only 100 of 310 total aligned reads displayed.

TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGGGGGGGGCAGCGCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCG  >  NC_014034/2559825‑2560024
                                                                                                    |                                                                                                      
  gCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCGGGGGGGTTGCGC.GTCAGCGCAAgcgggg                                                                                                    <  1:4452187/101‑1 (MQ=255)
  gCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAgcgggg                                                                                                    <  1:5832287/101‑1 (MQ=255)
     cagTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGc                                                                                                 >  1:5643872/3‑101 (MQ=255)
      ggTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCa                                                                                                <  2:2803514/101‑1 (MQ=255)
      ggTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCa                                                                                                <  2:5631889/101‑1 (MQ=255)
      ggTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCa                                                                                                <  1:643794/101‑1 (MQ=255)
      ggTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCa                                                                                                >  1:1961994/1‑101 (MQ=255)
      ggTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCa                                                                                                <  1:3044616/101‑1 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAgc                                                                                              <  1:4909990/101‑1 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAgc                                                                                              <  1:3272399/101‑1 (MQ=255)
           ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcc                                                                                           >  2:3680148/1‑101 (MQ=255)
            gTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGccc                                                                                          >  1:6765384/1‑101 (MQ=255)
             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                         >  2:2107460/1‑101 (MQ=255)
             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                         >  1:6801352/1‑101 (MQ=255)
             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                         <  1:4302860/101‑1 (MQ=255)
             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                         >  2:4146700/1‑101 (MQ=255)
              ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGccccc                                                                                        <  1:2842512/101‑1 (MQ=255)
              ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGccccc                                                                                        <  2:2604647/101‑1 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcccccc                                                                                       <  2:5414821/101‑1 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcccccc                                                                                       >  2:1222486/1‑101 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCGcccccc                                                                                       <  2:6793068/101‑1 (MQ=255)
                ccTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAgagatcggaa                                                                                      >  2:6722552/1‑92 (MQ=255)
                   gACGGGATGGTGTCCTTCGCGCCGCAGCAGGCCGGCTTTTCCTGGGGCGAAC.CGCCGGGGGGTTGCGC.GGCAGCGCAAGCGGGGGGCAGCGccccccgaag                                                                                   <  1:1742952/101‑5 (MQ=255)
                    acgggATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAgc                                                                                <  2:220596/98‑1 (MQ=255)
                    aCAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAgc                                                                                >  2:4636825/1‑101 (MQ=255)
                     cAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAgcg                                                                               >  1:5729226/1‑101 (MQ=255)
                      aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAgcgc                                                                              >  1:2369055/1‑101 (MQ=255)
                         aTTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTg                                                                           <  2:3014871/101‑1 (MQ=255)
                           tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAg                                                                         <  2:5783086/101‑1 (MQ=255)
                           cgtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAg                                                                         >  1:895002/2‑101 (MQ=255)
                               tCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGgcgc                                                                     >  1:408447/1‑101 (MQ=255)
                               tCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGgcgc                                                                     >  2:6583875/1‑101 (MQ=255)
                               cccTCCGCCCCGCGGC.GGCCCCGTTTTCCCTGGGCCAAACCGCGGGGGGGGGGCGG.GCCGGCG.AAGGGGGGGGCCCCCCCCCCCC.GAAGCGCCTGAGgcgc                                                                     <  1:1691650/100‑1 (MQ=255)
                                ccTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGcgcg                                                                    >  2:3172773/1‑101 (MQ=255)
                                ccTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGcgcg                                                                    >  1:5146514/1‑101 (MQ=255)
                                 cTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCgcgg                                                                   >  1:4867462/1‑101 (MQ=255)
                                 cTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCgcgg                                                                   >  1:3599734/1‑101 (MQ=255)
                                   tCGCGCCGCAGCCGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCgcggcg                                                                 >  1:6335961/1‑101 (MQ=255)
                                    cgcgCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGcggcgg                                                                >  1:5403674/1‑101 (MQ=255)
                                        gcgcAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGcct                                                            >  2:6186401/2‑99 (MQ=255)
                                          gcggcagGCCGGCTTTTCCTTGGGCGAAC.CGCGGGGGGGTTGCGC.TTCAGCGAAGGCGGGGGGCGGCG.CCCCCC.GAAGCGCCTGATGCGCGGCGGGCTTgc                                                          <  1:6868380/98‑1 (MQ=25)
                                          gcagcaGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCCTgc                                                          >  1:6373881/1‑101 (MQ=255)
                                          gcagcaGGCCCGCTTTTCCTGGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgc                                                          <  2:3968850/101‑1 (MQ=255)
                                           cagcagGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                         >  2:3352624/1‑101 (MQ=255)
                                             gcagGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCCGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccg                                                       >  1:1363323/1‑101 (MQ=255)
                                              cagGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                      >  1:966737/1‑101 (MQ=255)
                                               agGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTggccgcc                                                     >  2:6543208/1‑101 (MQ=255)
                                                gccccGCTTTTCCTGGGGCGAAA.CGCCGGGGGGTGGCGC.GTCAGCGCAGGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgccc                                                    <  1:4639700/99‑1 (MQ=25)
                                                 gCCCGTTTTTCCCTGGGGAGAA.CCGCGGGGGGTTGGGCGGCCGGCGAAGGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTgcccgccc                                                    <  2:3507982/101‑1 (MQ=12)
                                                  cccGCCTTTCCGGGGGCGAAA.CGCCGGGGGGTGGCGC.GTCAGCGCAAGCGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCaa                                                  <  1:2243763/101‑1 (MQ=25)
                                                   cggCTTTCCCTGGGGCGAAA.CGCCGGGGGGTGGCGC.GTCAGCGCAAGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAAc                                                 <  1:2746611/99‑1 (MQ=37)
                                                      cgtttctctGGGGGAAA.CCCCGGGGGGGGTGGC.GGGCGCGCGAGGGGGGGGGGCCGCCCCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACg                                                <  1:2592386/93‑1 (MQ=9)
                                                       ttttCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAg                                             >  1:1654207/1‑101 (MQ=255)
                                                       ttttCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAg                                             >  1:3817698/1‑101 (MQ=255)
                                                        tttCCTGGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAgg                                            <  2:5104541/101‑1 (MQ=255)
                                                         ttCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGGTTGCCCGCCCAACGAAgcg                                           >  2:6903186/1‑99 (MQ=255)
                                                             ttGGGCGAAC.CGCCGGGGGTTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCaa                                       <  2:4508816/101‑1 (MQ=25)
                                                              tGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGACGGGGCAac                                      >  1:7013148/1‑101 (MQ=25)
                                                              tGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAac                                      >  2:552641/1‑101 (MQ=255)
                                                               gggGGAAG.CGCCGGGGGGTGGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAacc                                     <  1:1190603/101‑1 (MQ=25)
                                                                 gCGAAC.CGCGGGGGGGTGGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaccac                                   <  2:4310454/101‑1 (MQ=25)
                                                                   gAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaccaccc                                 >  1:2706135/1‑100 (MQ=255)
                                                                   gAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                 >  2:6799253/1‑101 (MQ=255)
                                                                   gAAA.CCCCGGGGGGTTGCGC.GTCGGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                 <  2:2706033/101‑1 (MQ=25)
                                                                    aaa.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCtt                                >  1:5160924/1‑101 (MQ=255)
                                                                     aagccggggggggTTGCCC.GCCGGCGCAGGGGGGGGGCGGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCtt                                <  2:2368961/94‑1 (MQ=21)
                                                                     aaccGCGGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCtt                                <  2:5887165/98‑1 (MQ=25)
                                                                     aa.CGCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCTTc                               >  2:6037890/1‑101 (MQ=255)
                                                                      accGCGGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                               <  2:1810433/99‑1 (MQ=25)
                                                                        ggggggggggCGGCGC.GGCGGCG.AGGGGGGGGGCCCCCCCCCCCG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                             <  1:1500387/97‑1 (MQ=18)
                                                                        cGCGGGGGGGTCGCGG.GCCGGCGCAGGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                             <  2:1654146/101‑1 (MQ=21)
                                                                         gggggggggTCGCGC.GGCCGCGC.GGGGGGGGGCCCCCCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGt                            <  1:6162874/98‑1 (MQ=21)
                                                                         gCCGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACAAAGGCGCAACCACCTTCAGt                            >  2:7072277/1‑101 (MQ=255)
                                                                           cGGGGTGTTGCGC.GTCGGCGCAAGGGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                          <  2:5214373/101‑1 (MQ=255)
                                                                           cGGGGGGTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                          >  1:707399/1‑101 (MQ=255)
                                                                            ggggggTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCCGTCgg                         >  1:1855391/1‑101 (MQ=255)
                                                                            ggggggTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                         >  2:3924789/1‑101 (MQ=255)
                                                                               gggTTGCGC.GTCGGCGCAGGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                      <  2:6984090/101‑1 (MQ=25)
                                                                               gggTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGGGCAACCACCTTACGTCGGCtt                      >  2:5710064/1‑101 (MQ=25)
                                                                               gggTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                      <  1:5320856/101‑1 (MQ=255)
                                                                                ggTTGCGC.GTCGGCGCAAGGGGGGGGCGGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCGGTCGGCTTc                     <  1:5564896/101‑1 (MQ=25)
                                                                                 gtggcgc.GTCGGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                    <  2:4352491/98‑1 (MQ=25)
                                                                                 gTTGCGC.GTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                    >  1:5485044/1‑101 (MQ=255)
                                                                                  tggcgc.GTCAGCAAAGGGGGGGGGCGGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGa                   <  2:1541079/99‑1 (MQ=25)
                                                                                   ggcgc.GTCGGCGCAAGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt                  <  1:1026484/100‑1 (MQ=255)
                                                                                     ggc.gTCGGCGAAGGGGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcc                <  2:3562805/100‑1 (MQ=25)
                                                                                      gc.gTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCCTGGCCGCCCAACCAAGGCGCAACCACCCTCAGTCGGCCTCGATccc               >  1:6947133/1‑101 (MQ=25)
                                                                                        cgccggcgcAAGCGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcccc              <  1:4266814/96‑1 (MQ=37)
                                                                                         gTCAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca             >  1:1136376/1‑101 (MQ=255)
                                                                                         cgccggggcgggggggggCCGCCCCCCCCG.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcccc              <  1:2436275/91‑1 (MQ=25)
                                                                                          ccggcgcAGGCGGGGGCCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac            <  1:6584215/98‑1 (MQ=25)
                                                                                           cAGGGCAGGGGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc           <  2:894978/101‑1 (MQ=255)
                                                                                           cAGCGCAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc           >  2:1937426/1‑101 (MQ=255)
                                                                                            aGCCCAAGCGGGGGGCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca          <  2:3247883/101‑1 (MQ=255)
                                                                                             gcgcAAGCGGGGGGCGGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAg         <  2:1276004/101‑1 (MQ=255)
                                                                                               gcAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcg       >  1:3993512/1‑101 (MQ=255)
                                                                                                cAAGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc      >  2:781642/1‑101 (MQ=255)
                                                                                                 aaGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCCGcgcg     >  2:4838261/1‑101 (MQ=255)
                                                                                                 aaGCGGGGGGCAGCG.CCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGcgcg     >  2:4955810/1‑101 (MQ=255)
                                                                                                   ggggggggCAGC.CCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggc   <  1:333613/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTTGCGC.GTCAGCGCAAGGGGGGGGCAGCGCCCCCCC.GAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCG  >  NC_014034/2559825‑2560024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG