Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,595,353 G→C 100% V205V (GTG→GTC bglX → periplasmic beta‑glucosidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,595,3530G→C100.0% 995.4 286V205V (GTG→GTCbglXperiplasmic beta‑glucosidase
Reads supporting (aligned to +/- strand):  new base (151/134):  ref base (0/1):  total (151/135)

Only 100 of 310 total aligned reads displayed.

GCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTGGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCAA  >  NC_014034/2595255‑2595451
                                                                                                  |                                                                                                  
 cGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAg                                                                                                 <  1:1626576/101‑1 (MQ=255)
 cGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAg                                                                                                 <  1:4020051/101‑1 (MQ=255)
  ggATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAgg                                                                                                >  1:3098114/1‑101 (MQ=255)
   gATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGc                                                                                               >  2:4557605/1‑101 (MQ=255)
   gATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACACTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGc                                                                                               >  2:3902280/1‑101 (MQ=255)
    aTGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCg                                                                                              >  1:2840305/1‑101 (MQ=255)
     tGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCCACGGGCGCGATTATGCGGCGGTGGAGGCgg                                                                                             >  1:4508254/1‑100 (MQ=255)
      ggCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAg                                                                                            <  1:440761/101‑1 (MQ=255)
       gCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGc                                                                                           <  1:2100723/101‑1 (MQ=255)
        cGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcc                                                                                          >  1:3471326/1‑101 (MQ=255)
        cGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcc                                                                                          >  2:5047604/1‑101 (MQ=255)
        cGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcc                                                                                          >  2:5678860/1‑101 (MQ=255)
        cGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcc                                                                                          >  2:1078574/1‑101 (MQ=255)
          aCATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcccc                                                                                        <  1:3912245/101‑1 (MQ=255)
          aCATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcccc                                                                                        <  1:1948480/101‑1 (MQ=255)
          aCATGCCCCGGCCCGGCCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGcccc                                                                                        >  1:6861317/1‑101 (MQ=255)
           cATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcg                                                                                       <  2:2667209/101‑1 (MQ=255)
             tGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCcgcg                                                                                     <  1:2356076/101‑1 (MQ=255)
              ggcccggcccGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGa                                                                                    >  1:5172234/1‑101 (MQ=255)
                  cggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCt                                                                                >  1:5875693/1‑101 (MQ=255)
                   ggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTg                                                                               >  1:1567876/1‑101 (MQ=255)
                   ggcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTg                                                                               <  2:3672851/101‑1 (MQ=255)
                    gcccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGa                                                                              <  1:1107533/101‑1 (MQ=255)
                     cccgACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGAt                                                                             <  1:5914205/101‑1 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTTATGCg                                                                          <  2:3519591/101‑1 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                          >  2:5925643/1‑101 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                          >  2:4764219/1‑101 (MQ=255)
                        gACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCg                                                                          <  1:972820/101‑1 (MQ=255)
                           cGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGAt                                                                       <  2:1429734/101‑1 (MQ=255)
                           cGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGAt                                                                       >  1:5463687/1‑101 (MQ=255)
                            ggATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATc                                                                      >  1:5581488/1‑101 (MQ=255)
                             gatgatGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCGCGCGACGCTGATGCGGATCa                                                                     >  1:5625420/1‑101 (MQ=255)
                             gatgatGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCa                                                                     >  2:2140276/1‑101 (MQ=255)
                             gatgatGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCa                                                                     <  1:534810/101‑1 (MQ=255)
                             gatgatGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCGGATGCGGATCa                                                                     >  1:935841/1‑101 (MQ=255)
                              atgatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCAt                                                                    >  1:6232276/1‑101 (MQ=255)
                                gatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATgg                                                                  >  2:4168406/1‑101 (MQ=255)
                                gatgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATgg                                                                  >  1:3065535/1‑101 (MQ=255)
                                 atgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGGTCATggg                                                                 >  2:5587924/1‑100 (MQ=255)
                                  tgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAg                                                                <  2:362172/101‑1 (MQ=255)
                                  tgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAg                                                                >  2:1460049/1‑101 (MQ=255)
                                  tgGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAg                                                                >  2:2816768/1‑101 (MQ=255)
                                   ggCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGc                                                               <  2:73230/101‑1 (MQ=255)
                                   ggCGACCATGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGc                                                               <  2:949137/101‑1 (MQ=255)
                                    gCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGcc                                                              <  2:5567989/101‑1 (MQ=255)
                                      gACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCt                                                            >  1:4464163/1‑101 (MQ=255)
                                          cTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaa                                                        <  1:5047858/101‑1 (MQ=255)
                                           tGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaaa                                                       >  2:3091513/1‑101 (MQ=255)
                                           tGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCaaa                                                       <  1:1756678/101‑1 (MQ=255)
                                             aaGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATc                                                     <  2:1610172/101‑1 (MQ=255)
                                              agacttccgcgGGCTACGGCCTGTCCATCGGCGGGCGCGATTATGCGGCGGTCGCGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCg                                                    <  2:7053190/94‑1 (MQ=255)
                                               gCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGTCCTTCAAATCgg                                                   >  1:7021968/1‑101 (MQ=255)
                                                   ttCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCgg                                               >  2:3353366/1‑100 (MQ=255)
                                                      gcgGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTggc                                            >  2:6324593/1‑101 (MQ=255)
                                                       cgGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTggcg                                           >  2:1759013/1‑101 (MQ=255)
                                                       cgGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTggcg                                           >  2:5413450/1‑101 (MQ=255)
                                                       cgGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTggcg                                           >  2:2752640/1‑101 (MQ=255)
                                                        gggCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTggcgg                                          >  2:2426393/1‑101 (MQ=255)
                                                          gCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGgcggcg                                        >  1:2228406/1‑101 (MQ=255)
                                                            tACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCggg                                      >  1:5252915/1‑101 (MQ=255)
                                                            tACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCggg                                      >  2:148366/1‑101 (MQ=255)
                                                             aCGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCgggg                                     >  1:6422981/1‑101 (MQ=255)
                                                              cGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCggggg                                    <  1:2610642/101‑1 (MQ=255)
                                                               ggCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGc                                   <  2:1709324/101‑1 (MQ=255)
                                                               ggCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGc                                   <  2:1913160/101‑1 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                  <  1:3218862/101‑1 (MQ=255)
                                                                gCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGcc                                  <  2:133913/101‑1 (MQ=255)
                                                                  cTGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcgg                                >  1:1000063/1‑101 (MQ=255)
                                                                   tGTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggt                               <  1:3427388/101‑1 (MQ=255)
                                                                    gTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtg                              >  2:6512901/1‑101 (MQ=255)
                                                                    gTCCATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcgggg                              >  1:1170392/1‑99 (MQ=255)
                                                                     tCCATGGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgc                             <  2:6887415/101‑1 (MQ=255)
                                                                      ccATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcg                            <  1:1290800/101‑1 (MQ=255)
                                                                      caaTCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCACCGCGACGCTGATGCGGAGCATAGAGCCCTTCAAATCGGGCTCTGCGGAGAGGGCcgtggtg                            >  2:5833886/3‑96 (MQ=255)
                                                                       cATCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcgg                           <  1:3353489/101‑1 (MQ=255)
                                                                        aTCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCcggtgcggg                          >  2:6425367/1‑100 (MQ=255)
                                                                         tCGCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCgggggggggg                         >  2:2254791/1‑94 (MQ=255)
                                                                           gCCGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAt                       <  1:4185244/101‑1 (MQ=255)
                                                                            ccGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtg                      >  1:6604046/1‑101 (MQ=255)
                                                                             cGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtgg                     <  2:5607820/101‑1 (MQ=255)
                                                                             cGGGCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtgg                     <  2:6202511/101‑1 (MQ=255)
                                                                              gggCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtggt                    <  1:1460109/101‑1 (MQ=255)
                                                                              gggCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtggg                    >  1:2730596/1‑100 (MQ=255)
                                                                               ggCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtggtg                   <  2:5211896/101‑1 (MQ=255)
                                                                               ggCGCGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtggtg                   <  1:2783363/101‑1 (MQ=255)
                                                                                gcgcGATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGAtggtgg                  <  1:4764459/101‑1 (MQ=255)
                                                                                 cgcgATTAGGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGc                 <  2:1164689/101‑1 (MQ=255)
                                                                                  gcgATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGGGCGGGGGTGGTGGcc                >  2:5117100/1‑101 (MQ=255)
                                                                                    gATTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCtt              <  2:2211245/101‑1 (MQ=255)
                                                                                     aTTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCttt             >  2:1380165/1‑101 (MQ=255)
                                                                                     aTTATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGGGATGGTGGCCctt             >  2:2879287/1‑101 (MQ=255)
                                                                                      ttATGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCtttt            >  1:4870473/1‑101 (MQ=255)
                                                                                        aTGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTcc          <  2:4463924/101‑1 (MQ=255)
                                                                                        aTGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTcc          <  2:3925151/101‑1 (MQ=255)
                                                                                        aTGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGGGATGGTGGCCTTTTcc          >  2:2521996/1‑101 (MQ=255)
                                                                                         tGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcg         <  2:3446496/101‑1 (MQ=255)
                                                                                         tGCGGCGGTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCcg         <  2:1996532/101‑1 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa   <  1:3685789/101‑1 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa   >  1:6674351/1‑101 (MQ=255)
                                                                                               ggTCGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCa   <  2:3584437/101‑1 (MQ=255)
                                                                                                  |                                                                                                  
GCGGATGGCGACATGGCCCGGCCCGACCGGATGATGGCGACCCTGAAGCATTTCGCGGGCTACGGCCTGTCCATCGCCGGGCGCGATTATGCGGCGGTGGAGGCGAGCCCCGCGACGCTGATGCGGATCATGGAGCCCTTCAAATCGGGCGTGGCGGCGGGGGCCGGTGCGGTGATGGTGGCCTTTTCCGCGATCAA  >  NC_014034/2595255‑2595451

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG