Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	68.0	18	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (3/15):  ref base (0/0):  total (3/15)

CGCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGAGCC  >  NC_014034/2602952‑2603146                                                                                                   |                                                                                                 cgcgGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCgcga                                                                                                <  1:6554310/101‑1 (MQ=255)  gggggggggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGAc                                                                                               <  2:827549/99‑1 (MQ=255)  gggggggggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGAc                                                                                               <  1:2733815/99‑1 (MQ=255)  gggggcgggGCGGCCCCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGAc                                                                                               <  2:3954270/99‑1 (MQ=255)   cgggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACg                                                                                              <  2:4032614/101‑1 (MQ=255)     gggggggCGGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGcc                                                                                            <  1:4443220/98‑1 (MQ=255)      gcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                           <  1:808618/101‑1 (MQ=255)      gcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                           <  1:7047940/101‑1 (MQ=255)      gcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                           <  1:1250132/101‑1 (MQ=255)        ggggCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcgc                                                                                         <  2:1855864/101‑1 (MQ=255)         gggCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCcgcg                                                                                        <  2:4845439/101‑1 (MQ=255)             ggccgccGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGAGGCCAGCGGccc                                                                                    <  2:2625822/100‑1 (MQ=255)               ccgccgCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGccccc                                                                                  <  2:6008846/101‑1 (MQ=255)                cgccggGGCCGGCGAGCGGACCCCCACCCCCGCGCGGGGGGGGGCCGCGACCCCCCGCCCCCCCGCCCCCCAGCCCCCCGCCGCGACGCCCGCGGCCCCCg                                                                                 <  2:6713599/101‑1 (MQ=255)                                  gATCGACATCACCTCGCTGGCGGGGGGCGCGATCGACCGCGCCCCCCTCACCCAACCGCCCCCCCCGCCGGCCCCCGCCCCCCCCACCCCGcccccccccc                                                               >  2:261570/1‑95 (MQ=255)                                     cGACATCCCCGCGCTGGCGGGGGCCGCGATCGCCCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCccgccg                                                            <  2:6470439/101‑1 (MQ=255)                                                                        gcgcCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGgccgcc                         >  2:6550108/1‑101 (MQ=255)                                                                               cgccccccACCCCCCCGCCGCGCCGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgccc                  <  1:261574/96‑1 (MQ=255)                                                                                     ccAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGG.GGCGAAACCGGCCGCCCCCGCCCCGACCgg           >  1:4177300/1‑101 (MQ=255)                                                                                        gccgccCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGgcg         <  2:5724421/101‑1 (MQ=255)                                                                                            cccgccGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccga     >  2:6096192/1‑101 (MQ=255)                                                                                               gccgCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagcc  <  1:3253072/101‑1 (MQ=255)                                                                                                   |                                                                                                 CGCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGAGCC  >  NC_014034/2602952‑2603146 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 37 ≤  ATCG