Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,192 A→G 19.0% intergenic (‑168/+108) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1920A→G19.0% 142.8 ‑3.6 ‑0.2300intergenic (‑168/+108)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (35/22):  ref base (108/135):  total (143/157)
Fisher's exact test strand distribution p-value = 2.67e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.99e-01

Only 100 of 314 total aligned reads displayed.

CGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640093‑2640291
                                                                                                      |                                                                                                   
cgcgGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGa                                                                                                     <  1:6564442/101‑1 (MQ=255)
   ggcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCC.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCaa                                                                                                 >  2:4091759/1‑101 (MQ=37)
     gggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                                                                <  1:301025/100‑1 (MQ=37)
     cggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                                                                <  1:420231/101‑1 (MQ=37)
      ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  1:1046443/101‑1 (MQ=37)
          ggCGTGTCAACCGTGTCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  2:5593370/101‑1 (MQ=25)
          ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  2:120005/101‑1 (MQ=25)
           gCGTGTCAACGGTGGCGCATTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.G.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  1:501542/101‑1 (MQ=25)
           gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  2:4252412/101‑1 (MQ=25)
            ggtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                         <  1:2720101/100‑2 (MQ=25)
            cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                         <  1:2804442/101‑2 (MQ=25)
              tgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCCGCCCCCCCCTT.G.GCCCTTGGCCCATTCACCCCCCCAGGGAATTGGGGCAAgcaggaaaac                                                                                       >  2:5656694/1‑92 (MQ=255)
              tgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagac                                                                                       <  2:2659503/101‑4 (MQ=25)
                 cAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                                                   <  2:6793631/101‑1 (MQ=25)
                 cAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGCGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                                                   <  1:3406392/101‑1 (MQ=21)
                  aacgggggCGCTTTTACGCAGGCAAAAGGGGGGCGCTGCCGCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTAGAGCAAGATG.AAAGACCATg                                                                                  >  1:6853730/7‑101 (MQ=18)
                  aaCCGTGGCGCTTTTACGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  2:3487497/101‑1 (MQ=21)
                   aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTT.G.GCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCCTgg                                                                                 >  1:3622018/1‑101 (MQ=21)
                     cGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGt                                                                               <  1:2840868/101‑1 (MQ=25)
                      gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                              <  1:3152657/101‑1 (MQ=25)
                      gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                              <  1:1689096/101‑1 (MQ=21)
                      gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTT.G.GCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCATggggt                                                                              >  2:911040/1‑99 (MQ=21)
                       tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGGATTTGGGGCAAGATG.AAAGACCATGGGGTc                                                                             >  1:5531854/1‑101 (MQ=21)
                         gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                           <  1:3255382/101‑1 (MQ=21)
                           gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                         <  1:2331830/101‑1 (MQ=25)
                              tttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGc                                                                      <  2:5250840/101‑1 (MQ=25)
                                  cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTTCCCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCCTCTTGCCTcc                                                                  >  2:5841859/1‑100 (MQ=21)
                                  cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                  <  2:3302458/101‑1 (MQ=21)
                                   gcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaa                                                                 >  2:2512827/1‑101 (MQ=21)
                                    gccccgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                               <  1:1244791/95‑1 (MQ=9)
                                     ccccgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                              <  2:1964741/96‑1 (MQ=17)
                                      ggCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                              <  2:5519749/101‑1 (MQ=25)
                                      cccgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                             <  1:5924166/97‑1 (MQ=17)
                                       gCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             >  2:2837541/1‑101 (MQ=21)
                                       gCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             >  1:1892074/1‑101 (MQ=21)
                                       gCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  1:7128856/101‑1 (MQ=21)
                                       gCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  2:6205718/101‑1 (MQ=25)
                                        cgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                           >  1:1066645/3‑101 (MQ=17)
                                        cAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATACACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                            >  1:437305/1‑101 (MQ=21)
                                         aaaaGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                           <  2:3006014/101‑1 (MQ=25)
                                         aaaaGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                           <  1:6997950/101‑1 (MQ=25)
                                          aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         <  2:4635717/101‑1 (MQ=17)
                                          aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  2:642442/1‑101 (MQ=17)
                                          aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         <  1:580775/101‑1 (MQ=17)
                                          aaaAGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCCTGCCCCACATATCCCg                                                         >  2:508207/1‑101 (MQ=14)
                                           aaagggggCGCTGCCCCCCTGTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  1:6380098/4‑101 (MQ=18)
                                           aaagggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  2:3735280/4‑101 (MQ=17)
                                           aaGGGGGGCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATGCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                         <  1:3489945/101‑1 (MQ=21)
                                            aagggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                       <  2:5761377/99‑1 (MQ=18)
                                             ggggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       >  1:4425812/1‑101 (MQ=21)
                                             agggggCTCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      <  1:6489766/100‑1 (MQ=18)
                                              gggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     >  2:6270707/1‑101 (MQ=18)
                                               ggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                    >  1:3522438/1‑100 (MQ=18)
                                               ggggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                    >  1:570469/1‑101 (MQ=18)
                                                 ggCGCTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                  <  1:603994/101‑4 (MQ=21)
                                                 ggCGCTGCCCCCC.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                  <  1:4091953/101‑1 (MQ=25)
                                                    gcTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               <  2:797736/101‑7 (MQ=21)
                                                    gcTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                <  1:4051091/101‑1 (MQ=25)
                                                     cTGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               >  1:2076940/1‑101 (MQ=25)
                                                      tGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              <  2:3236123/101‑1 (MQ=25)
                                                      tGCCCCCCTCTT.G.GCCTTCGGCCAATTCACCCCCCCGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGACCCAAATATCTCgggggtgaggcga                                             >  2:2059231/1‑93 (MQ=21)
                                                        ccccccTCTT.GTC.CTTCGGCCAATTCACCCCCCGAGTTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGTGAGTCCTGa                                            <  1:1321150/101‑1 (MQ=21)
                                                        ccccccTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                            <  1:4128214/101‑1 (MQ=37)
                                                          ccccTCTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          >  1:1173241/1‑101 (MQ=37)
                                                              tctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      >  2:2725146/1‑101 (MQ=37)
                                                              tctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATCTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      <  1:883045/101‑1 (MQ=37)
                                                               ctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                     >  2:3942621/1‑101 (MQ=37)
                                                                  ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    >  2:5294920/6‑101 (MQ=37)
                                                                    ggccTTCGTGCAATTCCACCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  <  2:1859607/98‑1 (MQ=25)
                                                                      ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                >  1:5350422/2‑101 (MQ=37)
                                                                       cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               <  1:5289015/101‑1 (MQ=255)
                                                                        ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGTACGGGGGGCa                              <  2:2617275/101‑1 (MQ=37)
                                                                          cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  2:6643146/1‑101 (MQ=37)
                                                                           ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           >  2:4885366/1‑101 (MQ=37)
                                                                             gccatttcccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                         <  1:998709/93‑1 (MQ=37)
                                                                              cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                        >  2:2934627/1‑101 (MQ=37)
                                                                               aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  2:1616257/1‑101 (MQ=37)
                                                                                aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  1:6178895/1‑101 (MQ=37)
                                                                                 ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                     >  1:4775472/1‑101 (MQ=25)
                                                                                  tccccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    <  1:5373470/98‑1 (MQ=255)
                                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  2:6887980/4‑101 (MQ=25)
                                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  1:5135542/4‑101 (MQ=25)
                                                                                  tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  2:6000430/4‑101 (MQ=25)
                                                                                    cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                  <  2:1303642/100‑1 (MQ=255)
                                                                                     ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 <  2:5907108/101‑1 (MQ=255)
                                                                                     ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 >  1:678605/1‑101 (MQ=255)
                                                                                          cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            >  1:1489645/1‑101 (MQ=255)
                                                                                           gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           <  2:1054194/101‑1 (MQ=255)
                                                                                             ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         >  1:805513/1‑101 (MQ=255)
                                                                                             ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         >  1:369614/1‑101 (MQ=255)
                                                                                               tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       <  2:3655554/101‑1 (MQ=255)
                                                                                               tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  2:6047102/1‑101 (MQ=255)
                                                                                                aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCTCGGGTCTTtctc      <  1:6304721/101‑1 (MQ=255)
                                                                                                aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  2:6746902/1‑101 (MQ=255)
                                                                                                 tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     >  1:2686809/1‑101 (MQ=255)
                                                                                                  ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  2:6134877/1‑101 (MQ=255)
                                                                                                  ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    <  2:4364881/101‑1 (MQ=255)
                                                                                                   tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   >  1:546048/1‑101 (MQ=255)
                                                                                                   tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   >  2:4735081/1‑101 (MQ=255)
                                                                                                    ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:2274956/1‑101 (MQ=255)
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CGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640093‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG