Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,193 A→G 100% intergenic (‑169/+107) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1930A→G100.0% 950.3 300intergenic (‑169/+107)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (142/157):  ref base (0/0):  total (143/157)

Only 100 of 310 total aligned reads displayed.

GGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640096‑2640291
                                                                                                   |                                                                                                  
  gggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                                                                <  1:301025/100‑1 (MQ=37)
   ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  1:1046443/101‑1 (MQ=37)
    gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                              <  2:288441/101‑1 (MQ=37)
       ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCCTCGGCCAATTCACCCCCCCAGGTATTTGGAGCAAGATGaa                                                                                           >  2:5762913/1‑101 (MQ=25)
         ggtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                         <  1:2720101/100‑2 (MQ=25)
         cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGTAGCAAGATGaaag                                                                                         <  2:3729359/101‑2 (MQ=25)
         cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                         <  1:2804442/101‑2 (MQ=25)
             tCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGAGGaaagaaca                                                                                     >  2:2702655/1‑96 (MQ=21)
              cAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                                                   <  2:6793631/101‑1 (MQ=25)
               aacgggggCGCTTTTACGCAGGCAAAAGGGGGGCGCTGCCGCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTAGAGCAAGATG.AAAGACCATg                                                                                  >  1:6853730/7‑101 (MQ=18)
                aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                 >  2:3070104/1‑101 (MQ=21)
                aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTTG.GCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCCTgg                                                                                 >  1:3622018/1‑101 (MQ=21)
                  cGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGt                                                                               <  1:2840868/101‑1 (MQ=25)
                   gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                              <  1:1689096/101‑1 (MQ=21)
                   gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTTG.GCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCATggggt                                                                              >  2:911040/1‑99 (MQ=21)
                    tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                             <  2:4425391/101‑1 (MQ=25)
                       cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATc                                                                          <  1:5639255/101‑1 (MQ=25)
                          ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                       >  1:3236239/1‑101 (MQ=21)
                           tttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGc                                                                      <  2:4059642/101‑1 (MQ=21)
                             tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                    <  1:5031287/101‑1 (MQ=21)
                              gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                   >  2:1206730/1‑101 (MQ=21)
                               cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTTCCCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCCTCTTGCCTcc                                                                  >  2:5841859/1‑100 (MQ=21)
                               cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                  <  1:5441201/101‑1 (MQ=25)
                               cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                  <  2:1018980/101‑1 (MQ=25)
                                 gccccgaaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                               <  1:1244791/95‑1 (MQ=9)
                                  aGGCAAAAGGGGGGGGCTGCCCCCCTCTTG.GCCTTGGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                               <  1:3373679/101‑1 (MQ=21)
                                   cccgaaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                             <  1:4037723/97‑1 (MQ=17)
                                   cccgaaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                             >  1:5956748/5‑101 (MQ=17)
                                    gCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  1:7128856/101‑1 (MQ=21)
                                    gCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             <  2:4793909/101‑1 (MQ=25)
                                    gCAAAAGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                             >  1:1892074/1‑101 (MQ=21)
                                     cgaaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                           >  1:1066645/3‑101 (MQ=17)
                                     cAAAAGGGGGGCGCCGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCCAATATc                                                            >  2:1157057/1‑101 (MQ=18)
                                      gaaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCCAATATccc                                                          >  1:5718541/2‑101 (MQ=17)
                                      gaaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          >  2:3537200/2‑101 (MQ=17)
                                      gaaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:1377202/100‑1 (MQ=17)
                                      gaaaAGGGGGCGCTGCCCCCCTCTTG.GCCCTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  1:350845/100‑1 (MQ=18)
                                      aaaaGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                           <  1:6997950/101‑1 (MQ=25)
                                       aaaGGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                          >  1:2109252/1‑101 (MQ=21)
                                       aaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  1:4843105/1‑101 (MQ=17)
                                       aaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  2:6220702/1‑101 (MQ=17)
                                       aaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  2:642442/1‑101 (MQ=17)
                                       aaaAGGGGGCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCCTGCCCCACATATCCCg                                                         >  2:508207/1‑101 (MQ=14)
                                        aaagggggCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  2:1974105/4‑101 (MQ=17)
                                        aaagggggCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  1:4418942/4‑101 (MQ=17)
                                         aagggggCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                       >  2:4095922/3‑101 (MQ=18)
                                          ggggggCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       >  2:4667026/1‑101 (MQ=21)
                                           gggggCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                      <  2:5593605/101‑1 (MQ=25)
                                              ggCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                  >  1:6548651/1‑98 (MQ=21)
                                              ggCGCTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                   <  2:5568094/101‑1 (MQ=25)
                                               gcgcTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                  <  2:2941995/101‑1 (MQ=25)
                                                cgcTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                >  2:2820453/1‑96 (MQ=21)
                                                 gcTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               <  2:797736/101‑7 (MQ=21)
                                                  cTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               <  1:4087259/101‑1 (MQ=25)
                                                  cTGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGGcc                                               >  1:5228977/1‑101 (MQ=25)
                                                   tGCCCCCCTCTTG.GCCTTCGGCCAATTCCCCCCCCGGGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              <  1:6282146/101‑1 (MQ=25)
                                                   tGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              >  1:6366326/1‑101 (MQ=25)
                                                   tGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              <  1:2035131/101‑1 (MQ=25)
                                                   tGCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCCGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGACCCAAATATCTCgggggtgaggcga                                             >  2:2059231/1‑93 (MQ=21)
                                                    gCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                             <  1:5842161/101‑1 (MQ=37)
                                                    gCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                             >  2:5460582/1‑101 (MQ=25)
                                                    gCCCCCCTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGCATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                             >  2:4298084/1‑101 (MQ=25)
                                                     ccccccTCTTGTC.CTTCGGCCAATTCACCCCCCGAGTTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGTGAGTCCTGa                                            <  1:1321150/101‑1 (MQ=21)
                                                       ccccTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          >  1:1173241/1‑101 (MQ=37)
                                                       ccccTCTTG.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          >  2:338339/1‑101 (MQ=37)
                                                           tctTG.GCCTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      <  2:5272666/101‑1 (MQ=25)
                                                           tctTG.GCCTTCGGCCAATTCACCCCCCGAGGTATCTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      <  1:883045/101‑1 (MQ=37)
                                                                ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGATCAAGATG.AAAGACCATGGGTTCATCTTACCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  >  1:6413717/4‑101 (MQ=37)
                                                                ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  >  1:5316573/4‑101 (MQ=37)
                                                                 gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 >  1:4365103/3‑101 (MQ=37)
                                                                  ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                >  1:4118226/2‑101 (MQ=37)
                                                                   cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCTTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               <  1:6123021/101‑1 (MQ=37)
                                                                   cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  2:187385/1‑101 (MQ=37)
                                                                    ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                              <  2:6313213/101‑1 (MQ=255)
                                                                     tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                             >  2:3249094/1‑101 (MQ=37)
                                                                      cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            >  2:6643146/1‑101 (MQ=37)
                                                                           aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  1:3144712/1‑101 (MQ=255)
                                                                             ttCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                     <  1:4140203/101‑1 (MQ=255)
                                                                              tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  2:5661286/4‑101 (MQ=25)
                                                                              tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  2:6000430/4‑101 (MQ=25)
                                                                              tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  2:6887980/4‑101 (MQ=25)
                                                                                accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                  >  2:2650008/2‑101 (MQ=255)
                                                                                 ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 <  2:5907108/101‑1 (MQ=255)
                                                                                 ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 >  1:678605/1‑101 (MQ=255)
                                                                                   ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               <  2:2540065/101‑1 (MQ=255)
                                                                                   ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               >  1:5371678/1‑101 (MQ=255)
                                                                                      cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            >  1:1489645/1‑101 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  2:1619494/1‑101 (MQ=255)
                                                                                         ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         >  1:369614/1‑101 (MQ=255)
                                                                                         ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         >  1:805513/1‑101 (MQ=255)
                                                                                          gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        <  1:4667265/101‑1 (MQ=255)
                                                                                          gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        >  1:5484814/1‑101 (MQ=255)
                                                                                          gTATTTGGAGCAAGATG.AAAGACCAGGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        <  2:3894719/101‑1 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  2:6047102/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  1:348743/1‑101 (MQ=255)
                                                                                            aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  2:6746902/1‑101 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  2:6178576/101‑1 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     >  1:2686809/1‑101 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGCAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGACTTTCTCa     >  2:4928308/1‑101 (MQ=37)
                                                                                                ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:2274956/1‑101 (MQ=255)
                                                                                                   |                                                                                                  
GGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTG.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640096‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG