Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,198 A→G 18.3% intergenic (‑174/+102) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1980A→G18.3% 140.1 ‑3.2 ‑0.1310intergenic (‑174/+102)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (35/22):  ref base (117/136):  total (152/158)
Fisher's exact test strand distribution p-value = 4.12e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.58e-01

Only 100 of 325 total aligned reads displayed.

GGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640099‑2640297
                                                                                                    |                                                                                                   
ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                                     <  1:1046443/101‑1 (MQ=37)
   aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGa                                                                                                  <  2:6868710/101‑1 (MQ=37)
     gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                <  2:3141283/101‑1 (MQ=25)
         gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                            <  1:2794163/101‑5 (MQ=25)
          tCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGAGGaaagaaca                                                                                           >  2:2702655/1‑96 (MQ=21)
           cAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGCGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                                                         <  1:3406392/101‑1 (MQ=21)
            aacgggggCGCTTTTACGCAGGCAAAAGGGGGGCGCTGCCGCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTAGAGCAAGATG.AAAGACCATg                                                                                        >  1:6853730/7‑101 (MQ=18)
            aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                        <  1:1981738/101‑1 (MQ=25)
            aaCCGTGGCGCTTTTACGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                        <  2:3487497/101‑1 (MQ=21)
                gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTTGGCCTTCGGCCCATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCATggggt                                                                                    >  2:911040/1‑99 (MQ=21)
                 tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  1:5899415/101‑1 (MQ=25)
                 tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  2:4425391/101‑1 (MQ=25)
                 tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGGGCAAGATG.AAAGACCATGGGGTc                                                                                   >  1:5531854/1‑101 (MQ=21)
                   gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                 <  1:3255382/101‑1 (MQ=21)
                    cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATc                                                                                <  1:5639255/101‑1 (MQ=25)
                      cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCAGGGATTTTGAGCAAGATGAAAA.ACCATGGGGTCATCtt                                                                              >  2:3002164/1‑101 (MQ=21)
                       ttttGGGCAGGCAAAGGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGGTCATCTTg                                                                             <  1:6038095/101‑1 (MQ=18)
                          tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                          <  1:5031287/101‑1 (MQ=21)
                           gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         >  2:1206730/1‑101 (MQ=21)
                            cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                        <  1:768148/101‑1 (MQ=25)
                            cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                        <  2:1018980/101‑1 (MQ=25)
                              gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                     <  1:1244791/95‑1 (MQ=9)
                              gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                     >  1:4136595/7‑101 (MQ=14)
                               ccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                    <  2:1964741/96‑1 (MQ=17)
                                ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCTCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                    >  2:2023773/1‑101 (MQ=21)
                                ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                    <  1:7126188/101‑1 (MQ=25)
                                 gCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                   <  2:6205718/101‑1 (MQ=25)
                                 gCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                   <  2:4793909/101‑1 (MQ=25)
                                  cgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                 >  1:835644/3‑101 (MQ=17)
                                  cgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                 >  1:1066645/3‑101 (MQ=17)
                                  cAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                  <  2:5825609/101‑1 (MQ=25)
                                   aaaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                                 <  1:6997950/101‑1 (MQ=25)
                                    aaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                <  2:6771237/101‑1 (MQ=25)
                                    aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  2:5518016/1‑101 (MQ=17)
                                    aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  2:4635717/101‑1 (MQ=17)
                                    aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCCTGCCCCACATATCCCg                                                               >  2:508207/1‑101 (MQ=14)
                                     aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:3735280/4‑101 (MQ=17)
                                     aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              <  2:3904991/98‑1 (MQ=18)
                                     aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  1:2817513/4‑101 (MQ=17)
                                     aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  1:4418942/4‑101 (MQ=17)
                                     aaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATGCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                               <  1:3489945/101‑1 (MQ=21)
                                      aagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             <  2:5761377/99‑1 (MQ=18)
                                      aagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             >  2:4095922/3‑101 (MQ=18)
                                        gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                           >  2:6270707/1‑101 (MQ=18)
                                        gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                            <  2:5593605/101‑1 (MQ=25)
                                         ggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                          <  2:6343180/101‑2 (MQ=18)
                                         ggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                          >  1:3522438/1‑100 (MQ=18)
                                           ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                        >  1:6548651/1‑98 (MQ=21)
                                           ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                         <  2:5568094/101‑1 (MQ=25)
                                           ggCGCTGCCCCCC.CTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                        <  1:4091953/101‑1 (MQ=25)
                                             cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      >  1:1856682/1‑96 (MQ=21)
                                             cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      >  2:2820453/1‑96 (MQ=21)
                                             cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAgc                                                       >  2:2900934/1‑100 (MQ=25)
                                              gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                      <  1:4051091/101‑1 (MQ=25)
                                               cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                    >  1:2845683/1‑94 (MQ=25)
                                               cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGGcc                                                     >  1:5228977/1‑101 (MQ=25)
                                                tGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    >  1:6366326/1‑101 (MQ=25)
                                                 gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGCATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   >  2:4298084/1‑101 (MQ=25)
                                                    ccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                >  1:1173241/1‑101 (MQ=37)
                                                        tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATCTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                            <  1:883045/101‑1 (MQ=37)
                                                          ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                          >  2:5294920/6‑101 (MQ=37)
                                                           tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  2:5990172/5‑101 (MQ=37)
                                                            ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGATCAAGATG.AAAGACCATGGGTTCATCTTACCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                        >  1:6413717/4‑101 (MQ=37)
                                                            ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                        >  1:5316573/4‑101 (MQ=37)
                                                             gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                       >  1:4365103/3‑101 (MQ=37)
                                                              ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                      >  1:3698168/2‑101 (MQ=37)
                                                              ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                      >  2:6658813/2‑101 (MQ=37)
                                                               cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCTTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     <  1:6123021/101‑1 (MQ=37)
                                                               cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     >  2:187385/1‑101 (MQ=37)
                                                                ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                    <  2:5884693/101‑1 (MQ=255)
                                                                 tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                   >  2:3249094/1‑101 (MQ=37)
                                                                    gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                >  2:653908/1‑101 (MQ=37)
                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  1:2701572/1‑101 (MQ=255)
                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  2:1616257/1‑101 (MQ=37)
                                                                         ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           >  1:4943102/1‑101 (MQ=25)
                                                                          tccccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          <  1:5373470/98‑1 (MQ=255)
                                                                            cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        <  1:2079115/100‑1 (MQ=255)
                                                                            cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        <  2:1303642/100‑1 (MQ=255)
                                                                            accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        >  2:2650008/2‑101 (MQ=255)
                                                                             ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  1:3123931/101‑1 (MQ=255)
                                                                             ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       >  1:678605/1‑101 (MQ=255)
                                                                               ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                     <  2:2540065/101‑1 (MQ=255)
                                                                                  cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                  <  1:2461323/101‑1 (MQ=255)
                                                                                  cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                  >  2:7044362/1‑101 (MQ=255)
                                                                                   gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 <  1:3634379/101‑1 (MQ=255)
                                                                                     ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt               >  1:5745662/1‑101 (MQ=255)
                                                                                      gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              <  1:4667265/101‑1 (MQ=255)
                                                                                       tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  2:6047102/1‑101 (MQ=255)
                                                                                       tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             <  2:3655554/101‑1 (MQ=255)
                                                                                        aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCTCGGGTCTTtctc            <  1:6304721/101‑1 (MQ=255)
                                                                                         tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCGCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  1:1542019/1‑101 (MQ=37)
                                                                                         tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  2:6178576/101‑1 (MQ=255)
                                                                                         tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  2:5228712/101‑1 (MQ=255)
                                                                                         tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGCAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGACTTTCTCa           >  2:4928308/1‑101 (MQ=37)
                                                                                          ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa          >  1:1364661/1‑101 (MQ=255)
                                                                                           tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         >  1:546048/1‑101 (MQ=255)
                                                                                            ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  2:2274956/1‑101 (MQ=255)
                                                                                                cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    <  2:1173193/101‑1 (MQ=255)
                                                                                                 aaGATG.AAAGACCATGGGTTCATCTTCCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   <  1:2269614/101‑1 (MQ=255)
                                                                                                  aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  2:805489/101‑1 (MQ=255)
                                                                                                    |                                                                                                   
GGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640099‑2640297

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG