Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,216 C→T 16.0% intergenic (‑192/+84) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2160C→T16.0% 147.4 ‑0.4 ‑0.0350intergenic (‑192/+84)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/22):  ref base (168/126):  total (202/148)
Fisher's exact test strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 368 total aligned reads displayed.

TGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCG  >  NC_014034/2640116‑2640316
                                                                                                     |                                                                                                    
  gcgcTTGGGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                                    <  2:741756/101‑1 (MQ=21)
             gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                                        <  1:1244791/95‑1 (MQ=9)
             gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCAGCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                                        <  2:892674/95‑1 (MQ=9)
             gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                                        >  1:4136595/7‑101 (MQ=14)
              aGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                                        <  1:699049/101‑1 (MQ=21)
               ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                                       <  2:5519749/101‑1 (MQ=25)
               gccggaaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCGCCCCCCGGGATATTTGCGGGAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                                      <  1:6605090/93‑1 (MQ=12)
               cccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                                      <  2:5057407/97‑1 (MQ=17)
               cccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                                      >  1:5956748/5‑101 (MQ=17)
                gCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                                      <  2:6205718/101‑1 (MQ=25)
                gCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                                      >  1:1892074/1‑101 (MQ=21)
                ccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                                     >  1:912703/4‑101 (MQ=17)
                 cgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                                    >  1:835644/3‑101 (MQ=17)
                 cgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                                    <  1:3071685/99‑1 (MQ=17)
                 cAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTTCCTCaacaatc                                                                                     >  2:4820428/1‑96 (MQ=18)
                 cAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                                     <  2:5825609/101‑1 (MQ=25)
                 cAAAAGGGGGGCGCCGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCCAATATc                                                                                     >  2:1157057/1‑101 (MQ=18)
                  gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCCAATATccc                                                                                   >  1:5718541/2‑101 (MQ=17)
                   aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                                  >  1:5648133/1‑101 (MQ=17)
                   aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                                  >  2:642442/1‑101 (MQ=17)
                    aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                                 >  1:4418942/4‑101 (MQ=17)
                    aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                                 >  2:3735280/4‑101 (MQ=17)
                    aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                                 >  2:1974105/4‑101 (MQ=17)
                    aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                                 >  2:6031270/4‑101 (MQ=17)
                    aaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATGCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                                                  <  1:3489945/101‑1 (MQ=21)
                     aagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                                                <  2:5761377/99‑1 (MQ=18)
                       gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                              >  2:6270707/1‑101 (MQ=18)
                        ggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                                             <  2:6343180/101‑2 (MQ=18)
                        ggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGAGAAAGGA..TGGGGCTTATCTTGCCCCAAATATCCCgggggt                                                                             >  2:1039587/1‑100 (MQ=18)
                          ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                           <  2:2410834/101‑4 (MQ=21)
                          ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                            <  2:5568094/101‑1 (MQ=25)
                          ggCGCTGCCCCCC.CTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                                           <  1:4091953/101‑1 (MQ=25)
                           gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                                           <  2:2941995/101‑1 (MQ=25)
                            cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                         >  2:2820453/1‑96 (MQ=21)
                             gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                                         <  1:4051091/101‑1 (MQ=25)
                              cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                                       >  1:2845683/1‑94 (MQ=25)
                              cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                        >  1:2076940/1‑101 (MQ=25)
                               tGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                       <  1:3548698/101‑1 (MQ=25)
                                gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                      <  1:5842161/101‑1 (MQ=37)
                                gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                      >  2:5460582/1‑101 (MQ=25)
                                gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCAGGGATTAGGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                      >  2:3456559/1‑101 (MQ=25)
                                gCCCCCCTCTTGGCCTTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                      >  2:4188578/1‑101 (MQ=25)
                                        ctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                              >  2:3942621/1‑101 (MQ=37)
                                         ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                             >  2:5977121/6‑101 (MQ=37)
                                           ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGATCAAGATG.AAAGACCATGGGTTCATCTTACCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                                           >  1:6413717/4‑101 (MQ=37)
                                           ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                                           >  1:5316573/4‑101 (MQ=37)
                                            gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                          >  1:4365103/3‑101 (MQ=37)
                                              cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCTTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                        <  1:6123021/101‑1 (MQ=37)
                                              cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                        <  1:6804276/101‑1 (MQ=255)
                                               ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                                       <  2:5884693/101‑1 (MQ=255)
                                                tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                                      >  2:3249094/1‑101 (MQ=37)
                                                 cGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                                     <  1:5368060/101‑1 (MQ=255)
                                                 cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                                     >  2:257496/1‑101 (MQ=255)
                                                  ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                                    >  2:4885366/1‑101 (MQ=37)
                                                      aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                >  1:2701572/1‑101 (MQ=255)
                                                      aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                >  2:1616257/1‑101 (MQ=37)
                                                       aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                               <  1:5460854/101‑1 (MQ=255)
                                                        ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                              >  1:4775472/1‑101 (MQ=25)
                                                         tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                             >  1:5135542/4‑101 (MQ=25)
                                                         tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                             >  1:1151113/4‑101 (MQ=25)
                                                          caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                            >  2:5276975/3‑101 (MQ=255)
                                                           cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                           <  2:1303642/100‑1 (MQ=255)
                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                                    <  1:3634379/101‑1 (MQ=255)
                                                                    ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                                  >  2:4316225/1‑101 (MQ=255)
                                                                      tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                                <  2:3655554/101‑1 (MQ=255)
                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCTCGGGTCTTtctc                               <  1:6304721/101‑1 (MQ=255)
                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                               >  2:6746902/1‑101 (MQ=255)
                                                                        tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                              <  2:5228712/101‑1 (MQ=255)
                                                                         ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                             <  1:5435540/101‑1 (MQ=255)
                                                                         ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                             >  1:1364661/1‑101 (MQ=255)
                                                                         ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                             >  2:6134877/1‑101 (MQ=255)
                                                                           ggaggaaGATG.AAGGAACATGGGTTGATCTTGACTCAAATATCCCGGGGAGAGTCCTGAAAGGACTGGGGGCGGAGCCCGCCTCCCGGGTCTTTCTCAAGc                           <  2:4459259/96‑1 (MQ=255)
                                                                            tcttccgATCAAAAGCCCGTGGGTTCATCTGGCCTAAAATACCCCGGGGGGAGTCCGGAAGGGGGGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                           <  1:7090863/95‑1 (MQ=21)
                                                                             agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt                         >  2:1213591/3‑101 (MQ=255)
                                                                               cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                       <  1:743555/101‑1 (MQ=255)
                                                                                aaGATG.AAAGACCATGGGTTCATCTTCCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                      <  1:2269614/101‑1 (MQ=255)
                                                                                aaGATG.AAAGACCATGGGTTCATCTTACCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                      <  1:2806943/101‑1 (MQ=255)
                                                                                 aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg                     >  2:6901553/1‑101 (MQ=255)
                                                                                   gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg                    >  2:6606802/5‑101 (MQ=255)
                                                                                    atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc                   >  2:3223818/4‑101 (MQ=255)
                                                                                    atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc                   <  2:1792560/98‑1 (MQ=255)
                                                                                     tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt                  >  2:6827129/3‑101 (MQ=255)
                                                                                        aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg               >  2:5649428/1‑101 (MQ=255)
                                                                                        aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg               >  1:66788/1‑101 (MQ=255)
                                                                                          gACCATGGGTTCATCTTGCCTCAAATATCGCGGGGGGAGTCCTG.AAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc            <  1:1528572/101‑1 (MQ=255)
                                                                                          gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa             <  1:4885610/101‑1 (MQ=255)
                                                                                           aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc            >  2:1892775/1‑101 (MQ=255)
                                                                                             cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg          >  2:4713866/1‑101 (MQ=255)
                                                                                             cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg          <  2:2076860/101‑1 (MQ=255)
                                                                                             cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg          >  1:5540157/1‑101 (MQ=255)
                                                                                               tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGa        <  1:100445/101‑1 (MQ=255)
                                                                                                gggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGaa       <  1:6356175/101‑1 (MQ=255)
                                                                                                gggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGAAGAGCCCCCCTCCCGGGTGTTTCTCAAGCGTTCCGGCTCAGAACCGGaa       >  1:3418815/1‑101 (MQ=255)
                                                                                                 ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg      >  1:3272923/1‑101 (MQ=255)
                                                                                                 ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg      >  1:3767926/1‑101 (MQ=255)
                                                                                                 ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg      >  1:2477108/1‑101 (MQ=255)
                                                                                                 ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg      >  1:1656939/1‑101 (MQ=255)
                                                                                                  gTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGc     >  1:3188847/1‑101 (MQ=255)
                                                                                                   ttCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGcc    <  2:2701476/101‑1 (MQ=255)
                                                                                                    tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc   <  1:5275215/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
TGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCG  >  NC_014034/2640116‑2640316

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG