Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,691,600 T→C 2.4% Y417Y (TAT→TAC rluB → ribosomal large subunit pseudouridine synthase B

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,691,6000T→C 2.4% 4.3 ‑1.5 ‑0.3243Y417Y (TAT→TACrluBribosomal large subunit pseudouridine synthase B
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (120/117):  total (125/118)
Fisher's exact test strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.14e-01

Only 100 of 255 total aligned reads displayed.

AGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTACGCG  >  NC_014034/2691500‑2691699
                                                                                                    |                                                                                                   
 gCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATg                                                                                                    >  2:3387224/1‑101 (MQ=38)
  cccTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATgc                                                                                                   >  2:1661195/1‑101 (MQ=39)
   ccTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCATATGCGCCCCGCGATGGCGAGAAGAAGCCCTATgcg                                                                                                  >  1:6389608/1‑101 (MQ=255)
    cTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATgcgc                                                                                                 <  2:1857308/101‑1 (MQ=39)
    cTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATgcgc                                                                                                 <  2:2064802/101‑1 (MQ=39)
     tACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGcgcg                                                                                                >  2:7098796/1‑101 (MQ=39)
      aCGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGt                                                                                               >  2:549069/1‑101 (MQ=39)
       ccccgcgcgggCCGGCGACCGGAAGCCCTACGCGCGTCGCGAAGACGGCGGCCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTc                                                                                              <  2:3312579/96‑1 (MQ=11)
       cGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTc                                                                                              <  1:2109287/101‑1 (MQ=40)
             gcgcgACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAg                                                                                        <  1:5894816/101‑1 (MQ=40)
              cgcgACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGa                                                                                       <  2:7136400/101‑1 (MQ=39)
               gcgGCGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGTGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGAc                                                                                      <  1:4192711/101‑1 (MQ=255)
               gcgACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGAc                                                                                      <  2:3970317/101‑1 (MQ=40)
                  aCGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGc                                                                                   <  2:4163174/101‑1 (MQ=255)
                  aCGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGc                                                                                   <  1:2234918/101‑1 (MQ=255)
                   cGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCg                                                                                  <  1:4201502/101‑1 (MQ=255)
                    ggCGAGGGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGa                                                                                 <  2:2931860/101‑1 (MQ=255)
                     gCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGAc                                                                                <  1:6701667/101‑1 (MQ=255)
                        aGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGc                                                                             >  2:5667244/1‑101 (MQ=255)
                         gCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCa                                                                            >  2:646144/1‑101 (MQ=255)
                          cGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGCAGAACCCCTATCCGCGTCGCGATGACGGCGAcctgag                                                                           >  1:2750250/1‑97 (MQ=14)
                             aaGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGcc                                                                        <  1:6638654/101‑1 (MQ=255)
                              aGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGccc                                                                       <  2:3784864/101‑1 (MQ=40)
                                cccTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAACCCTACGCGCGTCGTGAAGACGGCGACCGCAAGCCCTa                                                                     >  2:3894457/1‑101 (MQ=255)
                                   tACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGc                                                                  <  1:3484241/101‑1 (MQ=39)
                                    aCGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGcc                                                                 <  2:6405488/101‑1 (MQ=39)
                                      gcgcgtcgtGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGcccc                                                               <  1:1007060/98‑1 (MQ=17)
                                      gCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGcccc                                                               <  1:549081/101‑1 (MQ=39)
                                      gCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAACCCTACGCGCGTCGTGAAGACGGCGACCGCAAGCCCTACGcccc                                                               <  1:3894620/101‑1 (MQ=255)
                                         cgtcgtGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCcgcg                                                            >  1:2463710/1‑101 (MQ=35)
                                            cgtGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGa                                                         >  1:1270494/1‑101 (MQ=35)
                                             gtGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGAc                                                        >  2:2954690/1‑101 (MQ=34)
                                             gtGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGAc                                                        >  1:6125488/1‑101 (MQ=34)
                                             gtGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGAc                                                        >  1:5248114/1‑101 (MQ=34)
                                              tGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACg                                                       <  2:5175815/101‑1 (MQ=33)
                                              tGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACg                                                       <  1:7099170/101‑1 (MQ=33)
                                               gAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACgg                                                      >  1:3009033/1‑101 (MQ=32)
                                               gAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACgg                                                      >  1:4632389/1‑101 (MQ=32)
                                                aaGACGGCGACCGCAAGCCCTATGCGCCCCGCGTTGGCGAGAAGAAGCCATATGCGCGTCGCGAGGACGGCGACCGCAAGCACNACGCCCCACGCGatgag                                                     >  2:3045981/1‑97 (MQ=11)
                                                aaGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGc                                                     <  2:2315374/101‑1 (MQ=32)
                                                 aGACGGCGTCCGCAATCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCg                                                    <  1:1238268/101‑1 (MQ=12)
                                                 aGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCg                                                    >  2:6905656/1‑101 (MQ=32)
                                                 aGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCg                                                    <  2:472271/101‑1 (MQ=32)
                                                   aCGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAg                                                  >  1:2537656/1‑101 (MQ=32)
                                                    cGGCGACCGCAAGCCCTATGCGCCGCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGc                                                 <  2:3841738/101‑1 (MQ=17)
                                                     ggCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCg                                                >  2:685806/1‑101 (MQ=32)
                                                     ggCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCg                                                >  2:4985651/1‑101 (MQ=32)
                                                     ggCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCg                                                >  1:6726432/1‑101 (MQ=32)
                                                          ccGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGc                                           <  2:919206/101‑1 (MQ=32)
                                                           cGCACGCCCTATGCGCCCCGCGATGGCGAGAGGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGcc                                          <  1:3258233/101‑1 (MQ=12)
                                                            gCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGccc                                         <  2:6002123/101‑1 (MQ=32)
                                                             cAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCt                                        <  2:6547935/101‑1 (MQ=32)
                                                             cAAGCCCTACGCGCCCCGCGATGGCGAGAAGAAACCCTAAGCGCGTCGTGACGACGGCGACCGCAAGCCCTCCGCCCAGCGAGCCGGGTAGCTGaaacaca                                        >  2:1144139/1‑96 (MQ=11)
                                                             cAAGCCCGAGGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCt                                        <  2:6273124/101‑1 (MQ=12)
                                                              aaGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGGGACCGCAAGCCCTACGCCCCGCGCGAGGGCGGGCGGAAGCCCtc                                       >  2:1909121/1‑100 (MQ=255)
                                                              aaGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGGGAGGAAGCCCtg                                       >  2:3861752/1‑100 (MQ=12)
                                                              aaGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTa                                       <  2:5848879/101‑1 (MQ=32)
                                                              aaGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTa                                       >  1:6550625/1‑101 (MQ=32)
                                                              aaGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTa                                       <  1:1388607/101‑1 (MQ=32)
                                                                gCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACg                                     >  1:3846876/1‑101 (MQ=32)
                                                                 cccTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGc                                    >  2:5913601/1‑101 (MQ=33)
                                                                  ccTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCTAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGcc                                   <  2:322737/101‑1 (MQ=17)
                                                                  ccTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGcc                                   >  1:6245059/1‑101 (MQ=33)
                                                                    tATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGGGAGCGGAAGCCCTACGCCcg                                 >  2:2313422/1‑101 (MQ=18)
                                                                    tATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCcg                                 >  1:4265874/1‑101 (MQ=33)
                                                                      tGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCcgtc                               <  2:4901662/101‑1 (MQ=32)
                                                                      tGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCcgtc                               >  1:453517/1‑101 (MQ=33)
                                                                      tGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCcgtc                               <  1:4750189/101‑1 (MQ=32)
                                                                           cccGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGaa                          <  2:5375200/101‑1 (MQ=32)
                                                                            ccGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAg                         <  1:2954799/101‑1 (MQ=32)
                                                                            ccGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAg                         <  1:3387348/101‑1 (MQ=32)
                                                                            ccGCGATGGCGAGAAGAAACCCTACGCGCGTCGCGAAGATGGCGACCGCAAGCCCTACGCGCACAGCGGTGGCGAGCAGAAACCCTATGcccccaggcagg                         >  1:6960885/1‑92 (MQ=11)
                                                                             cgcgATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGa                        <  1:6898044/101‑1 (MQ=32)
                                                                              gcgATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGAc                       >  1:498379/1‑101 (MQ=33)
                                                                               cgATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACTGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACg                      <  1:3698471/101‑1 (MQ=17)
                                                                               cgATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACg                      >  1:5449969/1‑101 (MQ=33)
                                                                                gATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACgg                     >  1:1734757/1‑101 (MQ=33)
                                                                                gATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACgg                     >  1:5847614/1‑101 (MQ=33)
                                                                                  tGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCg                   >  2:4346535/1‑101 (MQ=33)
                                                                                     cGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGAcc                <  2:1375900/101‑1 (MQ=32)
                                                                                     cGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGAcc                >  2:5594838/1‑101 (MQ=33)
                                                                                     cGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGAcc                >  1:529622/1‑101 (MQ=33)
                                                                                      gagaAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCg               <  2:2736555/101‑1 (MQ=32)
                                                                                      gagaAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCg               <  2:4632998/101‑1 (MQ=32)
                                                                                      gagaAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCCGGAAGACGGCGACCg               >  2:1243129/1‑101 (MQ=16)
                                                                                       agaagaAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGc              <  1:1024965/101‑1 (MQ=32)
                                                                                       agaagaAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGCGAAGACGGCGACCGc              >  1:3338826/1‑101 (MQ=17)
                                                                                          agaagCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAg           >  2:2540896/1‑101 (MQ=32)
                                                                                           gaagCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGc          >  1:2382916/1‑101 (MQ=32)
                                                                                            aagCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGcc         >  1:929017/1‑101 (MQ=32)
                                                                                             agCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGccc        >  1:2703966/1‑101 (MQ=32)
                                                                                              gCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCt       <  1:853054/101‑1 (MQ=32)
                                                                                               cccTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTa      >  2:2243496/1‑101 (MQ=32)
                                                                                               cccTACGCGCGTCGCGAAGACGGCGACCGCAAGCCCTATGCGCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTa      >  2:6641507/1‑101 (MQ=255)
                                                                                                ccTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTAc     <  2:3617429/101‑1 (MQ=33)
                                                                                                 cTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGACGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTCcg    >  1:4470081/1‑101 (MQ=12)
                                                                                                 cTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCTCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTAcc    >  2:2893732/1‑100 (MQ=18)
                                                                                                 cTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTAcg    <  2:6067891/101‑1 (MQ=34)
                                                                                                  tATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTAcgc   >  2:3267872/1‑101 (MQ=35)
                                                                                                   aTGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCGGTCGTGAAGACGGCGACCGCAAGCCCTAcgcg  >  1:4866091/1‑101 (MQ=25)
                                                                                                    |                                                                                                   
AGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTATGCGCCCCGCGATGGCGAGAAGAAGCCCTATGCGCGTCGCGAAGACGGCGACCGCAAGCCCTACGCCCCGCGCGACGGCGAGCGGAAGCCCTACGCCCGTCGTGAAGACGGCGACCGCAAGCCCTACGCG  >  NC_014034/2691500‑2691699

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG