Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,731,800 G→C 100% P509P (CCC→CCG RCAP_rcc02540 ← diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,731,8000G→C100.0% 1044.3 281P509P (CCC→CCGRCAP_rcc02540diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (157/123):  ref base (0/0):  total (158/123)

Only 100 of 290 total aligned reads displayed.

AGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGCC  >  NC_014034/2731706‑2731896
                                                                                              |                                                                                                
    cACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCgcgcgcgc                                                                                        >  2:1688549/1‑101 (MQ=255)
        cGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAg                                                                                    >  1:4550559/1‑101 (MQ=255)
          gTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCt                                                                                  >  2:5924674/1‑101 (MQ=255)
            cGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgc                                                                                <  1:6034842/101‑1 (MQ=255)
             gCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTgcg                                                                               >  1:2035615/1‑101 (MQ=255)
               ccAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGcc                                                                             >  1:6715152/1‑101 (MQ=255)
                  gCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCggg                                                                          >  2:6458466/1‑101 (MQ=255)
                  gCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCggg                                                                          <  1:981134/101‑1 (MQ=255)
                   cTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGc                                                                         <  2:3327755/101‑1 (MQ=255)
                    tGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGcc                                                                        <  1:1720485/101‑1 (MQ=255)
                    tGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGcc                                                                        <  1:1785632/101‑1 (MQ=255)
                    tGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGcc                                                                        <  1:31346/101‑1 (MQ=255)
                     gCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCCGGCCg                                                                       <  1:6868997/101‑1 (MQ=255)
                       gggCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGAc                                                                     >  2:3173447/1‑101 (MQ=255)
                        ggCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGAcc                                                                    >  2:585798/1‑101 (MQ=255)
                         gCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCt                                                                   <  2:3070888/101‑1 (MQ=255)
                         gCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGC..CGCGCGCGCGCAGCTGCGCCGGGCCGACCTgg                                                                 >  2:12789/1‑101 (MQ=255)
                           cGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTgg                                                                 >  2:5509548/1‑101 (MQ=255)
                           cGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTgg                                                                 <  1:1014892/101‑1 (MQ=255)
                            gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                                >  2:3349100/1‑101 (MQ=255)
                            gAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTggg                                                                >  1:6128847/1‑101 (MQ=255)
                             aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                               <  1:1810057/101‑1 (MQ=255)
                             aaaCGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCACGCGCGCGCAGCTGCGCCGGGCCGACCTGGgc                                                               >  2:5250309/1‑101 (MQ=255)
                                  gTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGa                                                          <  1:6367706/101‑1 (MQ=255)
                                    ccTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAg                                                        >  2:3974540/1‑101 (MQ=255)
                                    ccTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAg                                                        >  1:5851951/1‑101 (MQ=255)
                                     cTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAgg                                                       >  2:3717029/1‑101 (MQ=255)
                                      ttGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGa                                                      >  1:304403/1‑101 (MQ=255)
                                       tGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAt                                                     <  2:5409494/101‑1 (MQ=255)
                                        gATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAtt                                                    >  2:4160498/1‑101 (MQ=255)
                                        gATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGAtt                                                    >  1:4780163/1‑101 (MQ=255)
                                         aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                   >  2:6394991/1‑101 (MQ=255)
                                         aTCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTc                                                   <  2:197915/101‑1 (MQ=255)
                                           ctgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGGTTcgc                                                 >  1:3403882/1‑101 (MQ=255)
                                            tgctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCAGACCTGGGCGCGGAAGGATTcgcg                                                >  2:2228707/1‑101 (MQ=255)
                                              ctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAt                                              >  1:4077137/1‑101 (MQ=255)
                                              ctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAt                                              <  1:1599838/101‑1 (MQ=255)
                                              ctgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAt                                              >  2:2121029/1‑101 (MQ=255)
                                               tgGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGAtt                                             <  2:4700470/101‑1 (MQ=255)
                                                ggAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTg                                            >  2:4907733/1‑101 (MQ=255)
                                                 gAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                           <  1:6199447/101‑1 (MQ=255)
                                                 gAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                           <  2:6961055/101‑1 (MQ=255)
                                                 gAACGGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTgg                                           <  2:564469/101‑1 (MQ=255)
                                                  aaCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTggg                                          >  1:3703652/1‑101 (MQ=255)
                                                  aaCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTggg                                          >  1:4665779/1‑101 (MQ=255)
                                                   aCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGc                                         <  2:5805254/101‑1 (MQ=255)
                                                   aCTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGc                                         <  2:1034110/101‑1 (MQ=255)
                                                   aCTGGTCGATCAACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGc                                         >  1:59736/1‑101 (MQ=255)
                                                    cTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCa                                        >  2:454763/1‑101 (MQ=255)
                                                     tGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAg                                       >  1:27872/1‑101 (MQ=255)
                                                      ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGCTTCGCGATTGGGCAGc                                      >  2:1609764/1‑101 (MQ=255)
                                                      ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGc                                      >  1:774522/1‑101 (MQ=255)
                                                      ggTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGc                                      >  1:6620714/1‑101 (MQ=255)
                                                       gTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                     <  2:651763/101‑1 (MQ=255)
                                                       gTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                     >  2:2522646/1‑101 (MQ=255)
                                                       gTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGcc                                     >  1:2451083/1‑101 (MQ=255)
                                                        tcgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGccc                                    <  2:6050857/101‑1 (MQ=255)
                                                         cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                   >  1:538873/1‑101 (MQ=255)
                                                         cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                   >  2:2339450/1‑101 (MQ=255)
                                                         cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                   <  1:5047443/101‑1 (MQ=255)
                                                         cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                   >  1:4374507/1‑101 (MQ=255)
                                                         cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                   <  2:6098404/101‑1 (MQ=255)
                                                         cgatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCg                                   >  1:4164062/1‑101 (MQ=255)
                                                          gatcgaCAGGAAGCCCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                  <  1:2305486/101‑1 (MQ=255)
                                                          gatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                  >  2:2025935/1‑101 (MQ=255)
                                                          gatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                  >  1:4208619/1‑101 (MQ=255)
                                                          gatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                  >  2:2901001/1‑101 (MQ=255)
                                                          gatcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCgg                                  >  2:7084263/1‑101 (MQ=255)
                                                           atcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGt                                 >  1:3904366/1‑101 (MQ=255)
                                                            tcgaCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTc                                <  2:6738457/101‑1 (MQ=255)
                                                              gaCAGGAAGCCCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGGTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAg                              <  1:6068578/101‑1 (MQ=255)
                                                               aCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgc                             >  2:424976/1‑101 (MQ=255)
                                                               aCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgc                             <  1:6666375/101‑1 (MQ=255)
                                                               aCAGGAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAgc                             >  1:6786905/1‑101 (MQ=255)
                                                                  ggAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGca                          >  1:3212729/1‑101 (MQ=255)
                                                                  ggAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGca                          >  1:5584483/1‑101 (MQ=255)
                                                                   gAAGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcat                         <  1:5319475/101‑1 (MQ=255)
                                                                     agcccTGCTGCAAGAGAGCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatca                       <  2:4837641/98‑1 (MQ=255)
                                                                     aGACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatca                       <  2:1395347/101‑1 (MQ=255)
                                                                      gACCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGcatcat                      >  2:6259850/1‑101 (MQ=255)
                                                                       aCCTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATg                     <  2:5085474/101‑1 (MQ=255)
                                                                        ccTGCTGCAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGc                    <  1:581204/101‑1 (MQ=255)
                                                                        ccTGCTGCAAGAGATCGCCCCCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCCTGc                    <  1:6849238/101‑1 (MQ=255)
                                                                          tgctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGt                  <  2:4208424/101‑1 (MQ=255)
                                                                          tgctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGt                  <  1:6738534/101‑1 (MQ=255)
                                                                           gctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGACGCGCCGCGCCGACCTGGGCGCGGCAGGATTCGCGATTGGGCAGCCCGACCAGCGCATCATGCGTc                 >  2:817052/1‑101 (MQ=255)
                                                                            ctgcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcg                >  1:6985949/1‑101 (MQ=255)
                                                                              gcAAGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTcgcg              >  2:1840392/1‑101 (MQ=255)
                                                                                aaGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGc            >  1:7022598/1‑101 (MQ=255)
                                                                                aaGAGATCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGc            >  1:2726638/1‑101 (MQ=255)
                                                                                  gagaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAt          >  2:2702066/1‑101 (MQ=255)
                                                                                   aggacGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg         <  1:1188968/97‑1 (MQ=255)
                                                                                   agaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtg         >  1:3945419/1‑101 (MQ=255)
                                                                                    gaTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtgg        <  1:2643512/101‑1 (MQ=255)
                                                                                    cgatctccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtgg        <  2:4468295/95‑1 (MQ=255)
                                                                                     aTCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggc       <  1:3558390/101‑1 (MQ=255)
                                                                                      tCGCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggct      <  1:2122445/101‑1 (MQ=255)
                                                                                        gCCCGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggctgg    >  1:1645227/1‑101 (MQ=255)
                                                                                         cccGCCGCGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGcgctgatctt   <  2:1179956/101‑10 (MQ=255)
                                                                                          ccGCCGCGCGCGCGCGCAGGTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCAtggagatcg  >  1:4468534/1‑95 (MQ=255)
                                                                                              |                                                                                                
AGATCACCCGGTCGCCCAGCTGCGGGCCGAAACGGTCCTTGATCTGCTGGAACTGGTCGATCGACAGGAAGACCTGCTGCAAGAGATCGCCCGCGGGGCGCGCGCGCAGCTGCGCCGGGCCGACCTGGGCGCGGAAGGATTCGCGATTGGGCAGCCCGGTCAGCGCATCATGCGTCGCGGCATGGCTGGCC  >  NC_014034/2731706‑2731896

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG