Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,740,181 G→T 7.4% G453C (GGC→TGC)  RCAP_rcc02544 → ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,740,1810G→T 7.4% 2.3 ‑2.9 ‑0.065G453C (GGC→TGC) RCAP_rcc02544ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (28/32):  total (33/32)
Fisher's exact test strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 230 total aligned reads displayed.

GCTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGATCGTC  >  NC_014034/2740081‑2740281
                                                                                                     |                                                                                                       
gcTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGCCCGGCTT.g                                                                                                         >  1:412336/1‑101 (MQ=255)
  tCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.c                                                                                                      <  1:6626893/101‑1 (MQ=255)
  tCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.c                                                                                                      <  2:1616491/101‑1 (MQ=255)
   cGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.cc                                                                                                     <  1:1976550/101‑1 (MQ=255)
   cGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCCT.gggc                                                                                                      >  1:3613025/1‑99 (MQ=255)
    gATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGGGACCGACTGGGACCGGATCAACGACCGGCTT.GG.Gc.g                                                                                                   >  1:4007008/1‑101 (MQ=255)
    gATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.gggcc                                                                                                     >  2:896686/1‑98 (MQ=255)
     aTGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.Tc.gc                                                                                                  >  1:3739057/1‑101 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCg.cca                                                                                             <  1:5506479/101‑1 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCg.cca                                                                                             <  2:86362/101‑1 (MQ=255)
         ccGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCCT.TG.CC.GCg.cca                                                                                             >  2:4800653/1‑101 (MQ=255)
           gTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCg.ccagc                                                                                           <  2:3523219/101‑1 (MQ=255)
            tCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.TC.GCc.ccgcgc                                                                                          >  2:4587490/1‑97 (MQ=255)
            tCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCg.ccagcc                                                                                          <  2:1366266/101‑1 (MQ=255)
             cTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCg.ccagcca                                                                                         <  2:213442/101‑1 (MQ=255)
             cTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCg.ccagcca                                                                                         <  1:3219907/101‑1 (MQ=255)
             cTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCg.ccagcca                                                                                         <  1:6009190/101‑1 (MQ=255)
              ttGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.CC.CCg.cccgccac                                                                                        >  1:1294588/1‑100 (MQ=255)
              ttGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.ccagccag                                                                                        <  2:5925375/101‑1 (MQ=255)
                ggCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAgcg                                                                                      <  2:4387753/101‑1 (MQ=255)
                   cGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGcgccg                                                                                   <  2:5221275/101‑1 (MQ=255)
                     ggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGTCTT.GG.CC.GCG.CCAGCCAGCgccgcc                                                                                 >  1:5862582/1‑101 (MQ=255)
                     ggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCCGCCAGGgccgcc                                                                                 >  1:2379291/1‑101 (MQ=255)
                     ggTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCgccgcc                                                                                 <  2:2010615/101‑1 (MQ=255)
                      gTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.CCGGCG.CC.GCCCCCgccgccc                                                                                >  1:1000467/1‑100 (MQ=255)
                        gCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.GC.GCG.CCCGCCCCCgcccccccc                                                                              >  1:1868213/1‑98 (MQ=255)
                         ctctCGGGCGAGATCTCCCCGGGGTGGGCTCGGGCGGCGGCGGGGACCGCCGGGGACCGAATCCCCGACCGCCTT.GG.CC.TCG.CCAGCCAGCGCCGCCACTa                                                                             <  2:6248997/101‑1 (MQ=255)
                         ctctCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TT.GC.GCG.CCCGCCCGCgccgccccca                                                                             >  1:1683774/1‑97 (MQ=255)
                          tctcGGGCGTGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.GC.GCG.CCCGCCCGCgcccccccccc                                                                            >  1:655867/1‑96 (MQ=255)
                          tctcGGGCGAGATCCCCCCCGAGGCGGCTCTGGCGGCGGCGGCGCCCGACTGGGACCGGATCCCCGCCCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTAc                                                                            <  1:4628460/101‑1 (MQ=255)
                          tctcGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCCGCGCCGCCCCTAc                                                                            >  1:6257231/1‑101 (MQ=255)
                          tccggggCGAGACCCCCCCCGGGGCGGCTCGGGCGGCGGCGGCGCCCGACTGGGCCCGGACCCCCGACGGGTTG.GC.CC.GCG.CCAGCCAGCGCCGCCACTAc                                                                            <  2:464762/97‑1 (MQ=255)
                           ctcGGGCGAGATCCCCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACGGGGACCGGATCCCCGCCCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTAcc                                                                           <  1:6741165/101‑1 (MQ=255)
                           ctcGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGGCGCCCCTAcc                                                                           >  2:6427458/1‑101 (MQ=255)
                            tcGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.TC.GCG.CCCGCCAGCGCCCCCCCCAccc                                                                          >  2:3417422/1‑100 (MQ=255)
                                 cGAGATCCCCCCCGCGGCGGCTGTGGCAGGGCCGGCGACCGACTGGGACCGGATCACCGCCCGGTTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAgg                                                                     <  1:2951763/101‑1 (MQ=255)
                                  gagaTCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.CC.GCG.GCCGGCCGCGGCGCCCCCAccccccggc                                                                    >  2:3997843/1‑94 (MQ=255)
                                   agaTCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCCGCCCGCGCGCCCCCCACCCCGAGGCg                                                                   >  2:3058829/1‑101 (MQ=255)
                                   agaTCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCCGCCCGCGCCGCCCCCAccccccggcc                                                                   >  1:5786817/1‑93 (MQ=255)
                                        ccccccGGGGCGGCTCGGGCGGCGGCGGCGCCCGACTGGGCCCGGATCCCCGACCGGCTT.GG.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATggg                                                              <  1:1210132/100‑1 (MQ=255)
                                        aCCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCCCTACCCCGAGGCGGTggg                                                              >  1:456231/1‑101 (MQ=255)
                                           ccgggggcggcTCTGGGGGGGGCGGCGCCCGACGGGGACCGGATCCCCGCCCGGCTT.GG.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCt                                                           <  1:713572/96‑1 (MQ=255)
                                            ccGGGGCGGCTCTGGCGGGGGCGGCGCCCGACGGGGACCGGACCCCCGCCCGCCTG.GG.CC.GCG.CCTGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTg                                                          <  1:6409633/101‑1 (MQ=255)
                                              gAGGAGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.GC.GCG.CCCGCCCGCGCCGCCCCCACCCCCAGGCGGCGGGGCGggg                                                        >  2:5186157/1‑100 (MQ=255)
                                               gggcggcTCGGGGGGCGGCGGCGACCGACGGGGCCCGGACCCCCGCCCGGCTG.GG.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAg                                                       <  1:5642547/100‑1 (MQ=255)
                                                 gcggcTCGGGGGGCGGGGGCGACCGACTGGGACCGGACCACCGCCCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAggc                                                     <  1:3827842/101‑1 (MQ=255)
                                                   ggcTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.CC.GCG.CCCGCCAGCGCCGCCACCACCCCGCGGCGGCGGGGGTGGGggcgg                                                   >  2:966128/1‑101 (MQ=255)
                                                    ccgcgggcggcggcggcGCCCAGCGGGGCCCGGTCCACCGACCGGCTG.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAggcggc                                                  <  2:5372701/96‑1 (MQ=255)
                                                       tgggcggcggggccGCCCGCCGGGGACGGTACCCCCGCCCGGCTG.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGcc                                               <  2:2256825/99‑1 (MQ=255)
                                                       ctGGCGGGGGCGGCGACCGACGGGGCCCGGATCCCCGCCCGGCTG.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGcc                                               <  2:192708/101‑1 (MQ=255)
                                                       ctGGCGGCGGCGGCGCCCGACGGGGCCGGGACCCCCGCCCGGTTT.GG.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGcc                                               <  2:3122513/101‑1 (MQ=255)
                                                       ctGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.TG.GC.GCG.CCCGCCAGCGCCGCCCCCACCCCGAGGCGGTGGGGGTGGGGGGGGGGcc                                               >  1:7120698/1‑101 (MQ=255)
                                                       ctGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCC.CCAGCCCGCGCCGCCACCACACCCGGGCGAGGGGGGGGGGggcgggggc                                               >  1:5712026/1‑97 (MQ=255)
                                                        tGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCa                                              >  1:2461746/1‑101 (MQ=255)
                                                        gggcggcggcggcGCCCGACGGGGCCGGGACCCCCGCCCGGCTG.GC.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCa                                              <  2:3860695/100‑1 (MQ=255)
                                                         ggcggcggcggcGCCCGCCGGGGCCCGGACCCCCGCCCGGCTG.GC.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCAt                                             <  2:7126965/101‑1 (MQ=255)
                                                          gggccggcggcgACGGCTTGGGCCGGGATCCCCGCCCGGTTG.GG.CC.CCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATg                                            <  2:5360984/97‑1 (MQ=255)
                                                               ggcggcgCCGGCCGGGGCCCGGACCCCCGCCCGGCTT.GC.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCt                                       <  1:2678845/101‑1 (MQ=255)
                                                                 gggcgCCCGCCGGGGCCCGGACCCCCG.CCGGGTT.GGCCC.CCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgt                                     <  1:4800896/100‑1 (MQ=255)
                                                                  ggcgCCCGACGGGGCCGGGACCCCCGCCGGGCTT.GC.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgtg                                    <  2:4419721/101‑1 (MQ=255)
                                                                  ggcgACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCtgtg                                    >  2:3673031/1‑101 (MQ=255)
                                                                   gcgACCGACTGGGACCGGATCACCGACCGGCTT.TG.CC.GCG.CCCGCCAGGGCCGCCCCCACCCCGAGGCGGTGGGGCTGGGGGGGGCGCCCCGAGCCCGTgg                                   >  1:2505472/1‑101 (MQ=255)
                                                                    cgcccgcgtGGGCCGGGACCCCCGCCCGGCTG.GC.CC.CCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGgc                                  <  2:4563738/93‑1 (MQ=255)
                                                                    cgcccgacGGGGCCGGGACCCCCGCCCGCTTG.GG.CC.CCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGgc                                  <  1:896710/98‑1 (MQ=255)
                                                                     cacggagtGGGCCGGGATCCCCGCCGGGTTT.GC.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGgcg                                 <  2:5070281/94‑1 (MQ=255)
                                                                      accgacTGGGACCGGATCACCGACCGGCTT.TG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGGGAGGGGGCTGGGGGGGGGGGCCTGAGCCTGTGgcgc                                >  2:1201879/1‑101 (MQ=255)
                                                                      accgacTGGGACCGGATCACCGACCGGCTT.TG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGGTGGGGGGGGCGCCACGGGGCCGTGgcgc                                >  2:2378430/1‑101 (MQ=255)
                                                                         gacTGGGACCGGATCACCGACCGGCTT.TG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGGTGGGGCTGGAGGCGGGGCCATGAGCCTGTGGGGGGGc                             >  2:2066676/1‑101 (MQ=255)
                                                                         gacTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCCGCCAGCGCCGCCCCTACCGCGAGGCGATGGGGCTGGGGGGGGGGCCCCGAGCCCGTGGCGCGGc                             >  1:6916448/1‑101 (MQ=255)
                                                                          gcggggACCGAACCACCGACCGCCTT.GG.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCa                            <  2:3263562/98‑1 (MQ=255)
                                                                          acTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCCCTACCCCGAGGGGATGGGGGTGGGGGGGGGGCCCTGAGCCCGTGGGGCGGCa                            >  1:2615507/1‑101 (MQ=255)
                                                                            tGGGACCGGATCACCGACCGGCTT.GT.CC.GCG.CCCGCCCGCGCCGCCCCTACCGCCGGGCGGGGGGGGTGGGGGCGGCGCCATGAGGCCGTGGGGCGGGAtt                          >  1:529029/1‑101 (MQ=255)
                                                                             gggACCGGATCACCGACCGGCTT.GG.CC.GCG.CCCGCCCGCGCCCCCACCACCCCGAGGCGATGGGGCTGGGGGGGGCGCCATGAGCCTGTGGGGCGGCAGTc                         >  2:6056453/1‑101 (MQ=255)
                                                                             gggACCGGACCCCGGCCCGGTTG.GC.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTc                         <  2:7086240/101‑1 (MQ=255)
                                                                              ggcccGGACCCCCGCCCGGCTG.GC.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCa                        <  1:4418002/98‑1 (MQ=255)
                                                                              ggACGGGATCCCCGCCGGGCTG.GC.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCa                        <  1:6191345/101‑1 (MQ=255)
                                                                              ggACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCGTTcg                        >  2:6444658/1‑100 (MQ=255)
                                                                               gccggaacccccgcccgGCTG.GC.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAg                       <  2:4892982/91‑1 (MQ=255)
                                                                                  cGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACAACCGCGCGGCGATGGGGCTGGGGGGGGGGCCCTGAGCCTGTGGCGCGGCATTCAgggg                    >  2:3452086/1‑101 (MQ=255)
                                                                                   ggATCACCGACCGGCTT.TG.CC.GCG.CCAGCCCGCGCCGCCCCTACCGCGGGGCGATGGGGCTGGGGGGGGGGCCCTGAGCCTGGGGGGCGGGATTCAggggg                   >  2:4069014/1‑100 (MQ=255)
                                                                                   ggACCACCGCCCGGCTG.GG.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGggc                   <  2:1294540/101‑1 (MQ=255)
                                                                                      tCACCGGGCGGGTTGGG.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGggcgg                 <  1:4222673/101‑1 (MQ=255)
                                                                                       ccccgcccgGCTG.GG.CC.GCG.CCGGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCt               <  2:4447952/99‑1 (MQ=255)
                                                                                       cACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCCGTGGCGCGGCCTTCAGGGGCGGCt               >  1:1177188/1‑101 (MQ=255)
                                                                                        ccgggccgGCTG.GG.CC.CCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTg              <  1:6851715/96‑1 (MQ=255)
                                                                                        accgaccgGCTT.GG.CC.CCG.GCCGCCACCGCCGCCCCATCCTCGAGGCGGTGGGGGTGGCGGGGGGGCCACGAGGCCTTGGGGCGGCCTTCAGCggcggccg              >  2:6581359/1‑99 (MQ=255)
                                                                                          cgcccgGCTG.GC.CC.GCC.CCAGCCGGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGtt            <  2:5472107/98‑1 (MQ=255)
                                                                                          cgaccgGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGGGGCCGtt            >  1:6150216/1‑101 (MQ=255)
                                                                                            gacgGCTT.TG.CC.GAGCCCGGCCGGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTg           <  2:447515/99‑1 (MQ=255)
                                                                                             ccgGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCCCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCt         >  1:5364693/1‑101 (MQ=255)
                                                                                               ggCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGa       >  2:6870658/1‑101 (MQ=255)
                                                                                               ggCTG.GC.CC.GCC.CCACCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGa       <  1:4882853/101‑1 (MQ=255)
                                                                                                 cTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtc     >  2:1790389/1‑101 (MQ=255)
                                                                                                 cTT.GC.CC.GCC.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtc     <  2:2556894/101‑1 (MQ=255)
                                                                                                  tt.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcg    >  2:5724106/1‑101 (MQ=255)
                                                                                                  tt.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTCGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcg    >  2:5251765/1‑101 (MQ=255)
                                                                                                   t.GG.CC.GCG.CCAGGCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcgt   >  1:5054754/1‑101 (MQ=255)
                                                                                                   t.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATCAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcgt   >  1:5518318/1‑101 (MQ=255)
                                                                                                     gg.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcgtc  >  2:2831343/1‑101 (MQ=255)
                                                                                                     gg.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGAtcgtc  >  2:1074647/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
GCTCGATGCCCGTCTTGGCCGGGTGCTCTCGGGCGAGATCACCCCCGAGGCGGCTCTGGCGGCGGCGGCGACCGACTGGGACCGGATCACCGACCGGCTT.GG.CC.GCG.CCAGCCAGCGCCGCCACTACCGCGAGGCGATGGGGCTGGAGGCGGCGCCATGAGCCTGTGGCGCGGCATTCAGGGGCGGCTGTTGCTGATCGTC  >  NC_014034/2740081‑2740281

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG