Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,854	C→G	100%	intergenic (‑186/+107)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,854	0	C→G	100.0%	152.0	52	intergenic (‑186/+107)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (17/34):  ref base (0/0):  total (17/35)

AATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGG  >  NC_014034/2747759‑2747949                                                                                                 |                                                                                                  aaggggAACCGCGCCCCGCGTTGCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                               <  1:3161895/97‑1 (MQ=25) aaTGGGAACCGCCCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                               <  1:6045925/101‑1 (MQ=25) aaTAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                               <  1:3692838/101‑1 (MQ=37) aaTAGGAACCGCGACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                               <  2:4453868/101‑1 (MQ=37) aaTAGAAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgag                                                                                               <  1:13313/101‑1 (MQ=25)  aTAGGACACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                              <  1:4871919/101‑1 (MQ=37)  aTAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCgaga                                                                                              <  2:4378050/101‑1 (MQ=37)   tAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                             <  1:4294205/101‑1 (MQ=37)   tAGGAACCGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                             <  2:5592964/101‑1 (MQ=37)   aaGGAAACGCCCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                             <  2:7014257/100‑1 (MQ=37)    agtaagcgcgCCCCTCGTCTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                            <  1:6597892/95‑1 (MQ=25)    aGGAAACGCGCCCCGCGTTGCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                            <  2:6869036/101‑1 (MQ=37)     ggAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                           <  1:2778250/101‑1 (MQ=25)     ggAAACGCGGCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                           <  1:3169872/101‑1 (MQ=37)     ggAAACGCGCCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                           <  1:1265745/101‑1 (MQ=25)     ggAAACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                           <  2:3232245/101‑1 (MQ=37)      gAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgcc                                                                                          >  2:5618293/1‑100 (MQ=255)        accgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                        <  2:6453239/99‑1 (MQ=25)        accgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcc                                                                                        <  2:5118470/99‑1 (MQ=25)         aCGGGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                       <  2:6831639/101‑1 (MQ=25)         aCGCGACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                       <  2:2901215/101‑1 (MQ=25)          cgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccg                                                                                      <  1:5611129/101‑1 (MQ=25)            cgccccCCGTCTCCCCGCCCCCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGTCGAGAAGcgcccgcc                                                                                    <  1:5618583/98‑1 (MQ=21)             gACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                                  >  1:5354158/1‑101 (MQ=255)             cccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                                  <  1:4853413/99‑1 (MQ=25)             cccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCccgcc.g                                                                                  <  1:4452096/99‑1 (MQ=25)              ccccctttttCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  2:401784/94‑1 (MQ=25)              ccccGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  1:6301453/100‑1 (MQ=255)               cccccttccccccGCCCGCAGCGCCCGTTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.ggg                                                                                <  1:650591/92‑2 (MQ=21)               cccGCGTTCCCCCGCCCGCGGCCCCCGGTGGCGGCATGCCCGCTTCAGCCCGCACCACAGGGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.ggg                                                                                <  2:5353886/101‑2 (MQ=21)                 cccgtccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                              <  1:5862280/94‑4 (MQ=21)                  ggttcgccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                             <  2:5536887/95‑5 (MQ=21)                  gggtccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                             <  1:1130576/95‑5 (MQ=21)                  gcttccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                             <  1:5763948/95‑5 (MQ=21)                  cgcctgccccGCCCGCGGCGCCCGGTTGCGTCATGACCGTTTCAGCCCGCACAACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                             <  2:3361139/95‑5 (MQ=17)                   ggTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                            <  1:1011417/100‑6 (MQ=21)                   cgTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                            <  1:3454380/101‑6 (MQ=25)                     tccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                          <  1:76691/98‑8 (MQ=21)                                                    gACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGGGGTGGCCGAGGGCCCTTGGGCCCCTCggggt                                            >  2:3280255/1‑100 (MQ=17)                                                    gACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGt                                           >  2:3177167/1‑101 (MQ=17)                                                                        caGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCa                       >  2:4313916/1‑101 (MQ=17)                                                                                   gcccggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGGCACAGGACCTCTGTGAAGCCTGGCCAAGGCg            >  1:613325/1‑101 (MQ=17)                                                                                    cccggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGa           >  1:4768487/1‑101 (MQ=18)                                                                                     ctgccccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt          >  2:3850297/9‑101 (MQ=18)                                                                                      cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg         >  1:6657914/1‑101 (MQ=18)                                                                                      cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg         >  2:2722409/1‑101 (MQ=18)                                                                                      cggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg         >  1:4581203/1‑101 (MQ=18)                                                                                       ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg        >  2:2062315/1‑101 (MQ=18)                                                                                       ggcccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg        >  2:757934/1‑101 (MQ=18)                                                                                       gacccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGCCTCAGGACCCCTGTGACGCATGGCCAAGGGGATgg        >  2:2899044/7‑101 (MQ=18)                                                                                        gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc       >  1:5403698/6‑101 (MQ=18)                                                                                           cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt    >  2:732402/3‑101 (MQ=21)                                                                                            ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg   >  1:2804317/2‑101 (MQ=21)                                                                                             gcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTgg  >  1:1332634/1‑101 (MQ=21)                                                                                                 |                                                                                                  AATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGG.CCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGG  >  NC_014034/2747759‑2747949 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG