Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,770,015 C→G 100% H311Q (CAC→CAG RCAP_rcc02581 → sensor histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,770,0150C→G100.0% 1015.0 270H311Q (CAC→CAGRCAP_rcc02581sensor histidine kinase
Reads supporting (aligned to +/- strand):  new base (142/128):  ref base (0/0):  total (142/128)

Only 100 of 282 total aligned reads displayed.

GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112
                                                                                                  |                                                                                                 
gagaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGt                                                                                                 <  2:1809490/101‑1 (MQ=255)
 agaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTg                                                                                                >  1:3746730/1‑101 (MQ=255)
 agaTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTg                                                                                                >  1:7133352/1‑101 (MQ=255)
   aTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGt                                                                                              <  1:3498727/101‑1 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            >  2:1735860/1‑101 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            >  1:6011894/1‑101 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            >  2:5905237/1‑101 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            <  1:2880137/101‑1 (MQ=255)
      ggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGa                                                                                           >  2:4452758/1‑101 (MQ=255)
       gCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGaa                                                                                          >  1:384176/1‑101 (MQ=255)
       gCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGaa                                                                                          <  1:3578721/101‑1 (MQ=255)
        cAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAg                                                                                         <  1:2699962/101‑1 (MQ=255)
         aaGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        <  2:5769550/101‑1 (MQ=255)
         aaGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        >  2:2504858/1‑101 (MQ=255)
          aGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGAt                                                                                       >  2:1412191/1‑101 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     >  2:1902658/1‑101 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     <  2:522772/101‑1 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     <  1:1583438/101‑1 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     >  2:4329411/1‑101 (MQ=255)
             tgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGcc                                                                                    >  1:2988766/1‑101 (MQ=255)
               atCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGt                                                                                  <  2:6614947/101‑1 (MQ=255)
                 cGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgc                                                                                >  1:2333651/1‑101 (MQ=255)
                 cGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgc                                                                                <  2:5091590/101‑1 (MQ=255)
                   aCGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcgc                                                                              <  1:1293749/101‑1 (MQ=255)
                      ccTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGGGGAGATCTGCGCCTTTGTCGCGCAGGGGGCCGAAGATGCCGGGCGCGcc                                                                           >  1:913197/1‑101 (MQ=255)
                       cTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCg                                                                          <  2:462120/101‑1 (MQ=255)
                       cTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCg                                                                          <  1:4365088/101‑1 (MQ=255)
                        ttCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGCGGGCGAAGATGCCGTGCGCGCCgg                                                                         >  2:1467387/1‑101 (MQ=255)
                         tCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCggg                                                                        >  2:1057521/1‑101 (MQ=255)
                           cTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCa                                                                      <  2:6872236/101‑1 (MQ=255)
                            tGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAg                                                                     <  2:3832453/101‑1 (MQ=255)
                              aaTGTCGCGCGCGAAGAGGCGGGGGCGGGCGAGCCCGAACGGGGGGAGATCTGCGTCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGcg                                                                   >  2:1282225/1‑100 (MQ=255)
                              aaTGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGcc                                                                   <  1:1193878/101‑1 (MQ=255)
                              aaTGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGcc                                                                   >  1:6220127/1‑101 (MQ=255)
                               aTGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCg                                                                  >  1:6608620/1‑101 (MQ=255)
                               aTGGCGCGCGCGGAGGGGGGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCg                                                                  <  1:3303182/101‑1 (MQ=255)
                                 gTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGt                                                                >  2:4884248/1‑101 (MQ=255)
                                   cgcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGc                                                              <  2:6918611/101‑1 (MQ=255)
                                   cgcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGc                                                              <  2:5459252/101‑1 (MQ=255)
                                   cgcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGc                                                              <  2:2859762/101‑1 (MQ=255)
                                    gcgtgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCa                                                             <  1:5175159/101‑1 (MQ=255)
                                    gcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCa                                                             <  2:3133767/101‑1 (MQ=255)
                                    gcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCa                                                             <  2:5048810/101‑1 (MQ=255)
                                    gcgcgcgAAGAGGCGAGGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCa                                                             <  2:4920850/101‑1 (MQ=255)
                                       cgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCt                                                          <  1:4306544/101‑1 (MQ=255)
                                        gcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtg                                                         <  2:7047674/101‑1 (MQ=255)
                                         cgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtgg                                                        <  1:4951872/101‑1 (MQ=255)
                                          gAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGGGCAGCtggg                                                       >  2:2288342/1‑100 (MQ=255)
                                            agagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtggtgg                                                     >  1:790954/1‑101 (MQ=255)
                                             gagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGc                                                    <  1:4799807/101‑1 (MQ=255)
                                               ggCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCt                                                  >  2:2595728/1‑101 (MQ=255)
                                               ggCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCt                                                  <  2:4183268/101‑1 (MQ=255)
                                               ggCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCt                                                  <  1:2207951/101‑1 (MQ=255)
                                                gCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCCCCGGGCAGCCGGTGCAGCTGGTGGCCTg                                                 <  1:5065539/101‑1 (MQ=255)
                                                 cGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGc                                                <  1:3994658/101‑1 (MQ=255)
                                                  gATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcc                                               >  1:1752671/1‑101 (MQ=255)
                                                     ggcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCg                                            <  2:75200/101‑1 (MQ=255)
                                                     ggcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGGGCAGCTGGTGGCCTGCCCCg                                            <  2:4247425/101‑1 (MQ=255)
                                                       cgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGAt                                          <  2:3282208/101‑1 (MQ=255)
                                                          gcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcg                                       >  1:1347043/1‑101 (MQ=255)
                                                           ggAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcgc                                      <  1:3737413/100‑1 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     >  2:4767868/1‑101 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     >  1:2931710/1‑101 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     <  1:4017791/101‑1 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATACCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     >  2:2774231/1‑101 (MQ=255)
                                                                 cGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGc                                >  1:5806469/1‑101 (MQ=255)
                                                                 cGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGc                                >  2:3434980/1‑101 (MQ=255)
                                                                 cGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGc                                >  1:5492011/1‑101 (MQ=255)
                                                                  gAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCa                               >  1:7032735/1‑101 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              >  2:1430120/1‑101 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGCTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              <  1:3631100/101‑1 (MQ=255)
                                                                      gggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCg                           <  2:5576268/101‑1 (MQ=255)
                                                                      gggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCg                           >  1:6445562/1‑101 (MQ=255)
                                                                       ggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCgg                          >  2:3991799/1‑101 (MQ=255)
                                                                       ggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCgg                          <  1:5741055/101‑1 (MQ=255)
                                                                        gTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCggg                         >  1:686322/1‑100 (MQ=255)
                                                                          ggAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgc                       >  2:5683416/1‑101 (MQ=255)
                                                                          ggAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgc                       >  2:4389614/1‑101 (MQ=255)
                                                                          ggAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgc                       <  1:5661034/101‑1 (MQ=255)
                                                                           gagaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgct                      >  1:4068890/1‑101 (MQ=255)
                                                                            agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctg                     >  1:1405574/1‑101 (MQ=255)
                                                                            agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctg                     <  1:3107206/101‑1 (MQ=255)
                                                                            agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctg                     >  2:6073819/1‑101 (MQ=255)
                                                                             gaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctgc                    <  2:6420885/101‑1 (MQ=255)
                                                                             gaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctgc                    >  2:3058663/1‑101 (MQ=255)
                                                                               tCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTgcgc                  >  1:6770822/1‑101 (MQ=255)
                                                                                cTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGcc                 >  2:6651384/1‑101 (MQ=255)
                                                                                 tGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGccc                <  1:2845468/101‑1 (MQ=255)
                                                                                      cTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtg           <  1:4452980/101‑1 (MQ=255)
                                                                                      cTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtg           <  1:1925315/101‑1 (MQ=255)
                                                                                        ttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcg         >  1:1849649/1‑101 (MQ=255)
                                                                                        ttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcg         <  1:1057556/101‑1 (MQ=255)
                                                                                          gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgct       <  1:2453773/101‑1 (MQ=255)
                                                                                          gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgct       <  2:2022403/101‑1 (MQ=255)
                                                                                            cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGGCCCGATGCGCCGGTGCAGGCGGGGCTTCGCTCCGAtccgctgg     >  2:157997/1‑100 (MQ=255)
                                                                                            cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgc     >  2:1165178/1‑101 (MQ=255)
                                                                                            cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgc     >  1:4889324/1‑101 (MQ=255)
                                                                                            cgcgCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgc     >  1:4730996/1‑101 (MQ=255)
                                                                                             gcgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgcg    >  1:1679489/1‑101 (MQ=255)
                                                                                             gcgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgcc    >  2:4074185/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG