Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,884,906 T→C 100% K71E (AAG→GAG)  RCAP_rcc02692 ← organic solvent tolerance protein family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,884,9060T→C100.0% 1175.7 312K71E (AAG→GAG) RCAP_rcc02692organic solvent tolerance protein family
Reads supporting (aligned to +/- strand):  new base (163/149):  ref base (0/0):  total (163/149)

Only 100 of 318 total aligned reads displayed.

CTGCACCAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTTGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCGCCGCCAGCG  >  NC_014034/2884808‑2885001
                                                                                                  |                                                                                               
cTGCACCAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGa                                                                                               <  1:6914214/101‑1 (MQ=255)
cTGCACCAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGa                                                                                               >  1:1908758/1‑101 (MQ=255)
 tGCACCAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTGATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGAc                                                                                              <  1:3739944/101‑1 (MQ=255)
 tGCACCAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGAc                                                                                              >  1:1147424/1‑101 (MQ=255)
  gccccAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACa                                                                                             <  2:1804275/98‑1 (MQ=255)
  gccccAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACa                                                                                             <  2:1656024/98‑1 (MQ=255)
  gCACCAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACa                                                                                             >  2:6574397/1‑101 (MQ=255)
    cccAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATg                                                                                           <  2:6409067/100‑1 (MQ=255)
     ccAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATgg                                                                                          <  1:2497464/101‑1 (MQ=255)
      cAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGc                                                                                         >  2:2492451/1‑101 (MQ=255)
      cAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGc                                                                                         <  2:2948315/101‑1 (MQ=255)
        gCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGccc                                                                                       <  2:7014373/101‑1 (MQ=255)
        gCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGccc                                                                                       <  2:4526010/101‑1 (MQ=255)
        gCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGccc                                                                                       <  1:3029492/101‑1 (MQ=255)
         ccGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCg                                                                                      <  1:4034291/101‑1 (MQ=255)
         ccGGATCGGCCCCCCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCg                                                                                      <  2:7116563/101‑1 (MQ=255)
          cGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGa                                                                                     >  2:3739340/1‑101 (MQ=255)
          cGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGa                                                                                     >  2:2226985/1‑101 (MQ=255)
           ggATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAg                                                                                    <  1:4607032/101‑1 (MQ=255)
            gATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAgg                                                                                   <  2:2760837/101‑1 (MQ=255)
              tCGGCCCCGCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCg                                                                                 <  2:6804684/101‑1 (MQ=255)
               cGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGa                                                                                >  1:2140556/1‑101 (MQ=255)
                  ccccTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCa                                                                             <  1:54180/101‑1 (MQ=255)
                  ccccTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCa                                                                             <  1:2603756/101‑1 (MQ=255)
                   cccTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAg                                                                            <  2:3068213/101‑1 (MQ=255)
                    ccTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGc                                                                           >  1:5816938/1‑101 (MQ=255)
                    ccTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGc                                                                           <  1:5029032/101‑1 (MQ=255)
                      tCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcg                                                                         >  2:5119364/1‑101 (MQ=255)
                       cGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcgg                                                                        >  1:2051909/1‑101 (MQ=255)
                        gATCGGCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcggt                                                                       >  1:6861172/1‑101 (MQ=255)
                        gATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcggt                                                                       >  2:3925378/1‑101 (MQ=255)
                         aTCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcggtc                                                                      >  1:5653010/1‑101 (MQ=255)
                         aTCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcggtc                                                                      >  1:3078878/1‑101 (MQ=255)
                          tCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcggtcg                                                                     >  1:101272/1‑101 (MQ=255)
                            gCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCcggtcggt                                                                   <  2:5555445/101‑1 (MQ=255)
                             ccAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTg                                                                  <  2:4150853/101‑1 (MQ=255)
                             ccAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTg                                                                  <  1:3924986/101‑1 (MQ=255)
                                gCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCg                                                               <  1:4260739/101‑1 (MQ=255)
                                 ccGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGGCGGGGCCgg                                                              >  2:3464681/1‑101 (MQ=255)
                                  cGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGt                                                             <  2:6325628/101‑1 (MQ=255)
                                  cGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGt                                                             <  1:3158219/101‑1 (MQ=255)
                                      cGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGAcc                                                         <  2:4880842/101‑1 (MQ=255)
                                        cTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTc                                                       <  2:5844763/101‑1 (MQ=255)
                                         tGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCg                                                      >  2:1174959/1‑101 (MQ=255)
                                         tGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCg                                                      <  2:4021293/101‑1 (MQ=255)
                                          ggCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGa                                                     >  1:3791597/1‑101 (MQ=255)
                                           gCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGAt                                                    <  1:6815198/101‑1 (MQ=255)
                                           gCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGAt                                                    <  2:4963670/101‑1 (MQ=255)
                                           gCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGAt                                                    <  2:4629664/101‑1 (MQ=255)
                                             cTTGTCATAGGTGATGCGGTCGGCGGTCAGCCGGTGCCCGTGCCAATAGGCCGCGGCATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGGCCTCGATcc                                                  <  1:5121419/101‑1 (MQ=255)
                                             cTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATcc                                                  <  2:4238538/101‑1 (MQ=255)
                                             cTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATcc                                                  <  1:1987464/101‑1 (MQ=255)
                                               tGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCgg                                                <  2:2644264/101‑1 (MQ=255)
                                                gTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGt                                               >  1:4796611/1‑101 (MQ=255)
                                                 tCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTc                                              <  1:6423616/101‑1 (MQ=255)
                                                 tCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTc                                              <  1:5546380/101‑1 (MQ=255)
                                                 tCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTc                                              <  2:1559841/101‑1 (MQ=255)
                                                 tCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTc                                              <  1:146813/101‑1 (MQ=255)
                                                   aTAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCgg                                            >  1:3629845/1‑101 (MQ=255)
                                                    tAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGc                                           >  2:358582/1‑101 (MQ=255)
                                                      ggTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGANCTCGATCCGGTCGGCGa                                         >  2:777991/1‑101 (MQ=255)
                                                       gTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGag                                        <  2:5923835/101‑1 (MQ=255)
                                                         gATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCTGTCgagataga                                      >  1:1434287/1‑94 (MQ=255)
                                                          gtGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGagcag                                     <  1:1360398/100‑1 (MQ=255)
                                                              gcTGGGCGGCCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTc                                 <  2:3391613/101‑1 (MQ=255)
                                                              gcTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCgag                                 >  1:4381339/1‑99 (MQ=255)
                                                              gcTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTc                                 >  1:3252535/1‑101 (MQ=255)
                                                                 cggcggTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTcgcg                              >  2:182976/1‑101 (MQ=255)
                                                                   gcggTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGc                            <  1:3443498/101‑1 (MQ=255)
                                                                   gcggTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGc                            <  2:4421258/101‑1 (MQ=255)
                                                                    cggTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCa                           >  1:4961405/1‑101 (MQ=255)
                                                                     ggTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCAt                          <  2:2061164/101‑1 (MQ=255)
                                                                      gTCAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATc                         <  1:6720891/101‑1 (MQ=255)
                                                                        cAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCgg                       >  2:5270601/1‑101 (MQ=255)
                                                                        cAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCgg                       >  2:1019485/1‑101 (MQ=255)
                                                                        cAGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTCACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCgg                       <  1:6325555/101‑1 (MQ=255)
                                                                         aGCCGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGa                      >  1:237861/1‑101 (MQ=255)
                                                                           ccGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAAt                    <  1:2876555/101‑1 (MQ=255)
                                                                           ccGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAAt                    >  2:1394466/1‑101 (MQ=255)
                                                                            cGGTGCTCGTGCCGATAGACCGCGACATGGCCCGGGGCGATCAGCCGGTCGGTGCCGGTGACCACGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATa                   <  2:1721092/101‑1 (MQ=255)
                                                                            cGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATa                   >  1:3534733/1‑101 (MQ=255)
                                                                            cGGTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATa                   >  2:4381452/1‑101 (MQ=255)
                                                                             ggTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATaa                  <  1:4700836/101‑1 (MQ=255)
                                                                              gTGCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATaaa                 >  2:5897680/1‑101 (MQ=255)
                                                                                gCTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGGCGGCGAGCAGCGTCGCGGCATCGGAATAAAgc               >  2:3898702/1‑101 (MQ=255)
                                                                                 cTCGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAgcg              >  1:6642080/1‑101 (MQ=255)
                                                                                   cGTGCCAATAGCCCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAgcggc            <  2:5367172/101‑1 (MQ=255)
                                                                                   cGTGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAgcggc            >  2:270389/1‑101 (MQ=255)
                                                                                     tGCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGgcgc          <  1:874323/101‑1 (MQ=255)
                                                                                      gCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGcgcc         >  1:453834/1‑101 (MQ=255)
                                                                                      gCCAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGcgcc         <  1:1657847/101‑1 (MQ=255)
                                                                                       ccAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGcgccg        >  1:4113591/1‑101 (MQ=255)
                                                                                       ccAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGcgccg        >  2:6078129/1‑101 (MQ=255)
                                                                                        cAATAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGcgccgc       >  1:5301340/1‑101 (MQ=255)
                                                                                         aaTAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCgccgcc      <  2:4295882/101‑1 (MQ=255)
                                                                                         aaTAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCgccgcc      >  1:5861724/1‑101 (MQ=255)
                                                                                          aTAGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCGCCGCCa     <  2:3178807/101‑1 (MQ=255)
                                                                                           tAGACATCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCGCCGCCAg    >  1:4238409/1‑101 (MQ=255)
                                                                                            aGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCGCCGCCAgc   >  1:5172482/1‑101 (MQ=255)
                                                                                            aGACCTCGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCGCCGCCAgc   >  2:197967/1‑101 (MQ=255)
                                                                                                  |                                                                                               
CTGCACCAGCCGGATCGGCCCCTCGATCGCCAGCCGGTCGCTGGCCTTGTCATAGGTGATGCGCTCGGCGGTCAGCCGGTGCTCGTGCCAATAGACCTTGACATGGCCCGAGGCGATCAGCCGGTCGGTGCCGGTGACCTCGATCCGGTCGGCGAGCAGCGTCGCGGCATCGGAATAAAGCGGCGCCGCCAGCG  >  NC_014034/2884808‑2885001

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG