Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,891,981 T→G 100% S460A (TCG→GCG)  thiP → thiamine ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,891,9810T→G100.0% 1141.0 305S460A (TCG→GCG) thiPthiamine ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (170/135):  ref base (0/0):  total (170/135)

Only 100 of 314 total aligned reads displayed.

CCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891884‑2892078
                                                                                                 |                                                                                                 
ccGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGc                                                                                                >  1:3731531/1‑101 (MQ=255)
 cGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCt                                                                                               <  1:1158599/101‑1 (MQ=255)
  ggCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTg                                                                                              >  1:4955865/1‑101 (MQ=255)
    cTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtt                                                                                            <  2:1957641/101‑1 (MQ=255)
     tGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGttt                                                                                           <  2:3937675/101‑1 (MQ=255)
      gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                          <  2:342531/101‑1 (MQ=255)
      gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                          <  2:3157584/101‑1 (MQ=255)
      gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGtttt                                                                                          >  2:6315270/1‑101 (MQ=255)
      gCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGACGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGttct                                                                                          >  1:3883924/1‑99 (MQ=255)
        ggTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTcc                                                                                        >  2:4341106/1‑101 (MQ=255)
           tCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTGCCGAt                                                                                     >  2:70505/1‑101 (MQ=255)
            ccTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATg                                                                                    >  1:150362/1‑101 (MQ=255)
             cTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATgg                                                                                   >  2:3747887/1‑101 (MQ=255)
              tCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGc                                                                                  <  2:1405493/101‑1 (MQ=255)
              ccccccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGc                                                                                  <  1:5711878/98‑1 (MQ=255)
                  ccTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCa                                                                              >  1:1799542/1‑101 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGTTCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           >  1:5089740/1‑101 (MQ=255)
                     gCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           >  1:4493040/1‑101 (MQ=255)
                     gCCCCAGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGcc                                                                           >  1:4684090/1‑101 (MQ=255)
                      ccccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCa                                                                          <  2:6642730/101‑1 (MQ=255)
                       cccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAg                                                                         <  1:4194255/101‑1 (MQ=255)
                       cccGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAg                                                                         >  1:5963854/1‑101 (MQ=255)
                         cGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAc                                                                       <  2:6420512/101‑1 (MQ=255)
                         cGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAc                                                                       >  1:4085974/1‑101 (MQ=255)
                          ggCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcc                                                                      >  2:5910023/1‑101 (MQ=255)
                          ggCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcc                                                                      >  1:2972467/1‑101 (MQ=255)
                          ggCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcc                                                                      <  1:3602653/101‑1 (MQ=255)
                           gCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAccc                                                                     <  2:5144514/101‑1 (MQ=255)
                           gCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAccc                                                                     >  2:3299337/1‑101 (MQ=255)
                             tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   <  1:6170950/101‑1 (MQ=255)
                             tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   >  2:6127297/1‑101 (MQ=255)
                             tGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTg                                                                   <  2:2096878/101‑1 (MQ=255)
                              ggCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGAcggtgc                                                                  >  1:2179642/1‑96 (MQ=255)
                              ggCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGc                                                                  >  1:2894727/1‑101 (MQ=255)
                               gCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGcc                                                                 >  1:2430742/1‑101 (MQ=255)
                                  cGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCt                                                              >  1:3970677/1‑101 (MQ=255)
                                  cGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCt                                                              <  1:2825457/101‑1 (MQ=255)
                                   ggCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCtt                                                             >  2:5405611/1‑101 (MQ=255)
                                      cGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGcc                                                          <  1:1311130/101‑1 (MQ=255)
                                         cGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTc                                                       >  2:1056715/1‑101 (MQ=255)
                                         cGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTc                                                       >  2:6950144/1‑101 (MQ=255)
                                          gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCt                                                      <  2:4683851/101‑1 (MQ=255)
                                          gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCt                                                      >  2:3984692/1‑101 (MQ=255)
                                          gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCt                                                      >  1:1007515/1‑101 (MQ=255)
                                          gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCt                                                      <  1:3122153/101‑1 (MQ=255)
                                          gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGAAGCCAGACCCTGCCGCTTGCCGTCt                                                      >  2:1200755/1‑101 (MQ=255)
                                          gggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGAGCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCt                                                      >  2:3462864/1‑101 (MQ=255)
                                           ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     <  2:150359/101‑1 (MQ=255)
                                           ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     >  2:4134085/1‑101 (MQ=255)
                                           ggCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTa                                                     <  1:5763278/101‑1 (MQ=255)
                                             cTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATc                                                   <  1:3299453/101‑1 (MQ=255)
                                              tttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                  >  2:6814103/1‑101 (MQ=255)
                                              tttGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCa                                                  <  2:5998052/101‑1 (MQ=255)
                                                 gCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCt                                               >  1:2699413/1‑101 (MQ=255)
                                                    gcgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGAt                                            <  2:3553571/101‑1 (MQ=255)
                                                    gcgGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGAt                                            >  2:3359024/1‑101 (MQ=255)
                                                      ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGCTGGCCGCAGCCAGACCCCGCCGCTTCCCGTCTATCAGCTGATgg                                          >  1:6948051/1‑101 (MQ=255)
                                                      ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATgg                                          <  1:2293094/101‑1 (MQ=255)
                                                      ggggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTGTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATgg                                          >  2:2877853/1‑101 (MQ=255)
                                                       gggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATggg                                         >  1:3200952/1‑101 (MQ=255)
                                                        ggATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGc                                        <  2:2699317/101‑1 (MQ=255)
                                                         gATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCa                                       >  2:6540831/1‑101 (MQ=255)
                                                             gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                   >  1:6506008/1‑101 (MQ=255)
                                                             gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                   <  1:1857201/101‑1 (MQ=255)
                                                             gCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTa                                   >  2:1851415/1‑101 (MQ=255)
                                                              ccGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTAt                                  <  2:5594481/101‑1 (MQ=255)
                                                              ccGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTAt                                  <  2:6481265/101‑1 (MQ=255)
                                                              ccGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTAt                                  <  2:3214259/101‑1 (MQ=255)
                                                               cgcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATc                                 >  1:2502434/1‑101 (MQ=255)
                                                                gcgGCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCg                                <  2:5052899/101‑1 (MQ=255)
                                                                  ggCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCa                              <  2:220600/101‑1 (MQ=255)
                                                                  ggCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCa                              >  1:5492801/1‑101 (MQ=255)
                                                                  ggCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCa                              >  1:4746968/1‑101 (MQ=255)
                                                                   gCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCAt                             >  2:2325926/1‑101 (MQ=255)
                                                                   gCCTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCAt                             >  2:4827398/1‑101 (MQ=255)
                                                                    ccTTTTCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATg                            <  1:6324545/101‑1 (MQ=255)
                                                                      ttttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGa                          <  2:3883761/101‑1 (MQ=255)
                                                                       tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGac                         >  2:1807352/1‑100 (MQ=255)
                                                                       tttCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGAt                         >  2:3023039/1‑101 (MQ=255)
                                                                         tCGATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCa                       >  2:1791970/1‑101 (MQ=255)
                                                                          cGATGGGCCATCTGGGCGCCATCGCGCTGTGTTCCGATGGCCGCAGCCAGGCCCTGCCGCTTGCCGTCTTTCAGCTGATGGGCAGCTATCGCATGGATCAg                      <  2:1274889/101‑1 (MQ=255)
                                                                           gATGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTGTCAGCTGATGGGCAGCTATCGCATGGATCAgg                     >  1:3536535/1‑101 (MQ=255)
                                                                            aTGGGCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGc                    >  1:5257109/1‑101 (MQ=255)
                                                                              gggCGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCcg                  >  1:586791/1‑101 (MQ=255)
                                                                                 cGATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCgg               >  1:1413886/1‑101 (MQ=255)
                                                                                  gATCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCggg              >  1:2642535/1‑101 (MQ=255)
                                                                                    tCTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcg            >  2:5460438/1‑101 (MQ=255)
                                                                                     cTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcgc           <  2:2402933/101‑1 (MQ=255)
                                                                                     cTGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGgcgc           >  1:3857555/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  1:1689828/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  1:911474/1‑101 (MQ=255)
                                                                                      tGGGCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGcgcg          >  2:3674695/1‑101 (MQ=255)
                                                                                       gggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCgg         >  2:2017096/1‑101 (MQ=255)
                                                                                        ggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGc        >  1:6119026/1‑101 (MQ=255)
                                                                                        ggCGCCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGc        >  2:2641749/1‑101 (MQ=255)
                                                                                          cgcCATCGCGCTGTTTTCCGAGGGCCGAAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCtt      <  2:5678527/101‑1 (MQ=255)
                                                                                           gcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTg     >  2:4440747/1‑101 (MQ=255)
                                                                                           gcCATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTg     <  2:2524891/101‑1 (MQ=255)
                                                                                            ccATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGc    <  1:4343191/101‑1 (MQ=255)
                                                                                             cATCGCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGcc   >  2:4571754/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
CCGGCTGCGGTTCCTCACCCTGCCCCGGCTGGCCCGGCCGACGGGCTTTGCCGCGGGGATTGCCGCGGCCTTTTCGATGGGCGATCTGGGCGCCATCTCGCTGTTTTCCGATGGCCGCAGCCAGACCCTGCCGCTTGCCGTCTATCAGCTGATGGGCAGCTATCGCATGGATCAGGCCGCGGGCGCGGCTTGCCT  >  NC_014034/2891884‑2892078

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG