Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,732 C→G 2.7% intergenic (+75/‑85) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7320C→G 2.7% 15.3 ‑2.7 ‑0.1413intergenic (+75/‑85)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (9/2):  ref base (205/195):  total (215/198)
Fisher's exact test strand distribution p-value = 6.44e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.20e-01

Only 100 of 444 total aligned reads displayed.

CGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGA  >  NC_014034/2950632‑2950832
                                                                                                    |                                                                                                    
 gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGcccc                                                                                                     >  1:5565744/1‑101 (MQ=255)
 gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGcccc                                                                                                     >  1:3411221/1‑101 (MQ=255)
  cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                                    <  2:5664560/101‑1 (MQ=255)
  cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                                    <  1:3707002/101‑1 (MQ=255)
   aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                                   >  2:1077482/1‑101 (MQ=255)
   aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                                   >  1:6283974/1‑101 (MQ=255)
    atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                                  >  2:3816791/1‑101 (MQ=255)
      atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                                                <  2:3049164/101‑1 (MQ=255)
      atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                                                >  1:764917/1‑101 (MQ=255)
      atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                                                >  1:3992825/1‑101 (MQ=255)
      atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                                                >  1:1921173/1‑101 (MQ=255)
      atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                                                <  1:4143983/101‑1 (MQ=255)
        gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                                              <  1:932090/101‑1 (MQ=255)
         cGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATcc                                                                                             >  1:4582334/1‑101 (MQ=255)
          ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                                            <  1:6869592/101‑1 (MQ=255)
           gAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCg                                                                                           <  2:3411097/101‑1 (MQ=255)
            aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCgg                                                                                          >  1:647924/1‑101 (MQ=255)
            aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCgg                                                                                          <  2:2121438/101‑1 (MQ=255)
            aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCgg                                                                                          >  1:16675/1‑101 (MQ=255)
              cTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCgggg                                                                                        >  1:5212338/1‑101 (MQ=255)
              cTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCgggg                                                                                        >  2:3260972/1‑101 (MQ=255)
                 gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                                     <  1:2031535/101‑1 (MQ=255)
                  ttttCTCAAGCAAGCCGGGGGCTCGGCCCGCGGACCCCCGGGATATTTGAGGGAAGATGAAACCCGCCGCTTTCATCTTGTCCCAAATAGCCCGGGGGTGa                                                                                    <  2:6810309/100‑1 (MQ=21)
                  aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                    >  2:2010619/1‑101 (MQ=255)
                  aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                    >  2:5421047/1‑101 (MQ=255)
                  aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                    >  2:229114/1‑101 (MQ=255)
                  aTTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                    >  2:2633467/1‑101 (MQ=255)
                      cTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                                >  2:1908418/1‑101 (MQ=255)
                       tGAAGCAAGCCGTGGGCTCGGCCCCCGGACCCCCGGGATATGTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTg                                                                               <  2:6670383/101‑1 (MQ=255)
                        gAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATGCCGGGGGTGAATTTGc                                                                              >  2:4983839/1‑101 (MQ=255)
                         aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGcc                                                                             >  2:4813190/1‑101 (MQ=255)
                          agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCg                                                                            >  2:5160674/1‑101 (MQ=255)
                             aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCa                                                                         <  2:1643293/101‑1 (MQ=255)
                             aagcCGGGGGCTCTGCCCCCGGACCCCCGCGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCa                                                                         <  1:4510122/101‑1 (MQ=255)
                              agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAg                                                                        <  1:6017668/101‑1 (MQ=255)
                               gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGTGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAGTTTGCCGTCAgg                                                                       <  1:5066116/101‑1 (MQ=255)
                                ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGc                                                                      <  1:4802674/101‑1 (MQ=255)
                                 cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCa                                                                     <  1:3139898/101‑1 (MQ=255)
                                 cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCa                                                                     <  2:1292686/101‑1 (MQ=255)
                                  gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCaa                                                                    >  2:4637325/1‑101 (MQ=255)
                                  gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCaa                                                                    >  2:6338005/1‑101 (MQ=255)
                                    gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAag                                                                  <  1:251624/101‑1 (MQ=255)
                                      gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAagag                                                                <  2:6998762/101‑1 (MQ=255)
                                        tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAggg                                                              >  2:6883049/1‑101 (MQ=255)
                                        tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAggg                                                              >  1:1592381/1‑101 (MQ=255)
                                         ctGGCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATGTGCCGTCAGGCAAAGAgggg                                                             >  1:4369532/1‑101 (MQ=255)
                                         ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                                             <  2:3654797/101‑1 (MQ=255)
                                            cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                                          <  1:5844956/101‑1 (MQ=255)
                                            cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                                          <  2:3149308/101‑1 (MQ=255)
                                            cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                                          <  2:628732/101‑1 (MQ=255)
                                              tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAggg                                                       >  2:552963/7‑99 (MQ=2)
                                              tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcg                                                       >  1:3306343/7‑100 (MQ=2)
                                              tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcc                                                       >  1:262213/7‑100 (MQ=2)
                                                cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTc                                                      >  2:5774990/1‑101 (MQ=255)
                                                cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGCGAATTTGCCGTCAGGCAAAGACGGGGCAGCTc                                                      >  2:3851982/1‑101 (MQ=255)
                                                 ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGCGAATTGGCC.AAAGGCCAAGAGGGGGCAGCGccc                                                    >  2:797802/4‑101 (MQ=2)
                                                   aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcccc                                                   <  1:4624581/101‑1 (MQ=255)
                                                   aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccac                                                   <  2:4160544/101‑3 (MQ=255)
                                                    cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccccc                                                  <  1:1151494/101‑1 (MQ=255)
                                                    cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccccc                                                  <  1:4878694/101‑1 (MQ=255)
                                                     ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                                                 <  1:6924263/101‑1 (MQ=255)
                                                     ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                                                 <  1:6213104/101‑1 (MQ=255)
                                                        cGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcc                                              <  1:6402679/101‑1 (MQ=255)
                                                           gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                                           <  2:357716/101‑1 (MQ=255)
                                                            atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                                          >  2:6149607/1‑101 (MQ=255)
                                                             tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                                         >  1:3584354/1‑101 (MQ=255)
                                                              atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGTTGAATTTGCCTTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                        <  2:6439394/101‑1 (MQ=255)
                                                              atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                        <  2:4769110/101‑1 (MQ=255)
                                                              atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                        >  1:852380/1‑101 (MQ=255)
                                                               tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                                       <  1:1948780/101‑1 (MQ=255)
                                                                ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTga                                      >  2:2869235/1‑101 (MQ=255)
                                                                   aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                                   <  1:6779572/101‑1 (MQ=255)
                                                                     gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                 <  1:2325425/101‑1 (MQ=255)
                                                                     gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                 >  1:1257672/1‑101 (MQ=255)
                                                                       aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                               >  1:1015802/1‑101 (MQ=255)
                                                                       aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                               >  2:1490405/1‑101 (MQ=255)
                                                                         gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                             >  2:5226336/1‑101 (MQ=255)
                                                                         gATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCgg                             <  1:6110519/101‑1 (MQ=255)
                                                                          aTGAAACACGCCCCTTTCATCTTCCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                            <  2:1820727/101‑1 (MQ=255)
                                                                             aaaCACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                         <  2:3944025/101‑1 (MQ=255)
                                                                             aaaCACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                         <  1:6525753/101‑1 (MQ=255)
                                                                                cacGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                      <  2:6049007/101‑1 (MQ=255)
                                                                                 ccGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                     <  2:597638/100‑1 (MQ=255)
                                                                                 acGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa                     <  1:5421319/101‑1 (MQ=255)
                                                                                       cTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg               <  1:988614/101‑1 (MQ=255)
                                                                                       cTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg               >  1:3881682/1‑101 (MQ=255)
                                                                                        tttCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa              >  2:4389075/1‑101 (MQ=255)
                                                                                         ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa             >  1:2798916/1‑101 (MQ=255)
                                                                                          tCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg            <  1:1476092/101‑1 (MQ=255)
                                                                                             tCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc         >  1:5739697/1‑101 (MQ=255)
                                                                                             tCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc         <  1:704766/101‑1 (MQ=255)
                                                                                              cTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc        <  1:430797/101‑1 (MQ=255)
                                                                                              cTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc        <  2:2467362/101‑1 (MQ=255)
                                                                                              cTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc        >  2:4523614/1‑101 (MQ=255)
                                                                                               ttGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCg       <  2:6588569/101‑1 (MQ=255)
                                                                                                tGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc      >  1:5315922/1‑101 (MQ=255)
                                                                                                 gCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGcc     >  2:1699556/1‑101 (MQ=255)
                                                                                                  ccccAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt    <  1:1822499/101‑1 (MQ=255)
                                                                                                  ccccAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt    <  1:1364118/101‑1 (MQ=255)
                                                                                                   cccAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg   <  2:6201523/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGA  >  NC_014034/2950632‑2950832

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG