BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,960,499	A→G	100%	E123E (GAA→GAG)	hisP →	polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,960,499	0	A→G	100.0%	1228.3	325	E123E (GAA→GAG)	hisP	polar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand): new base (185/140): ref base (0/0): total (185/140)

Only 100 of 329 total aligned reads displayed.


GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596


   cGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGcc                                                                                              <  1:135927/101‑1 (MQ=255)


   cGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGcc                                                                                              >  2:2249385/1‑101 (MQ=255)


    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             <  1:324563/101‑1 (MQ=255)


    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             >  1:5138010/1‑101 (MQ=255)


    gggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCg                                                                                             <  2:1767203/101‑1 (MQ=255)


     ggCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCgg                                                                                            >  1:4788954/1‑101 (MQ=255)


      gcgcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGt                                                                                           <  2:5319951/101‑1 (MQ=255)


       cgcAGGTACCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTg                                                                                          <  1:2514309/101‑1 (MQ=255)


       cgcAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTg                                                                                          >  1:2707930/1‑101 (MQ=255)


         cAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGAc                                                                                        >  2:2644486/1‑101 (MQ=255)


          aGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACg                                                                                       >  1:2799057/1‑101 (MQ=255)


            gTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGt                                                                                     >  2:3035543/1‑101 (MQ=255)


            gTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGt                                                                                     >  1:1274292/1‑101 (MQ=255)


            gTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGt                                                                                     >  1:688862/1‑101 (MQ=255)


             tAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGtt                                                                                    <  2:2834296/101‑1 (MQ=255)


             tAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGtt                                                                                    <  2:2166065/101‑1 (MQ=255)


             tAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGCCGCCGGTGACGGtt                                                                                    >  1:5833029/1‑101 (MQ=255)


              aaCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTc                                                                                   <  2:2692508/101‑1 (MQ=255)


               aCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCt                                                                                  >  1:1506848/1‑101 (MQ=255)


               aCGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCt                                                                                  <  2:6450944/101‑1 (MQ=255)


                cgcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTg                                                                                 <  1:1792290/101‑1 (MQ=255)


                cgcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTg                                                                                 >  1:3532865/1‑101 (MQ=255)


                cgcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTg                                                                                 <  1:506827/101‑1 (MQ=255)


                 gcgGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTgg                                                                                >  2:4024881/1‑101 (MQ=255)


                    gTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGcc                                                                             >  1:3652139/1‑101 (MQ=255)


                      ttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGc                                                                           >  2:1367414/1‑101 (MQ=255)


                      ttCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGc                                                                           >  1:4939451/1‑101 (MQ=255)


                       tCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCa                                                                          <  2:5168913/101‑1 (MQ=255)


                          cACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAc                                                                       >  1:1926172/1‑101 (MQ=255)


                          cACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAc                                                                       >  1:6613664/1‑101 (MQ=255)


                          cACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAc                                                                       >  1:6152796/1‑101 (MQ=255)


                           tccAGCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAAcc                                                                      <  1:256212/100‑1 (MQ=255)


                            ccAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCg                                                                     <  2:1690487/101‑1 (MQ=255)


                             cAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCgc                                                                    >  1:4018436/1‑100 (MQ=255)


                              aaCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGc                                                                   >  1:2023915/1‑101 (MQ=255)


                              aaCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGc                                                                   <  2:614207/101‑1 (MQ=255)


                               aCCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGcc                                                                  <  2:5901150/101‑1 (MQ=255)


                                 cTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGa                                                                >  2:1855251/1‑101 (MQ=255)


                                  tGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGag                                                               >  1:5768137/1‑100 (MQ=255)


                                   gTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGagg                                                              >  2:5550617/1‑99 (MQ=255)


                                   gTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGagg                                                              >  1:910576/1‑99 (MQ=255)


                                   gTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGagg                                                              <  1:77847/101‑3 (MQ=255)


                                   gTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGagg                                                              <  2:3050958/101‑3 (MQ=255)


                                     cGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGagggc                                                            >  2:1306494/1‑97 (MQ=255)


                                      gATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCg                                                           >  2:1013181/1‑101 (MQ=255)


                                       aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                          <  2:6309024/101‑1 (MQ=255)


                                       aTGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGAAGATCCTGCAAAATGTCATGGAGGCGCAGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGa                                                          >  1:6321736/1‑101 (MQ=38)


                                         ggTGTTCCAGCAGTTCAACCTCTGGAGCCACATGAAGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAAc                                                        >  2:4620932/1‑101 (MQ=255)


                                            tcttccgatcttCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGc                                                     <  1:2747627/91‑1 (MQ=255)


                                             ttCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCt                                                    <  2:579331/101‑1 (MQ=255)


                                              tCCAGCCGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAAGGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTc                                                   <  1:1718704/101‑1 (MQ=255)


                                                cagcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGc                                                 >  1:4528168/1‑101 (MQ=255)


                                                  gcagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGccc                                               <  1:1246296/101‑1 (MQ=255)


                                                   cagTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCg                                              >  2:6571448/1‑101 (MQ=255)


                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         >  2:506039/1‑101 (MQ=255)


                                                        cAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGcc                                         <  1:3164550/101‑1 (MQ=255)


                                                         aaCCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCt                                        <  1:4280430/101‑1 (MQ=255)


                                                           ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                      <  2:4983993/101‑1 (MQ=255)


                                                           ccTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTc                                      <  2:4865770/101‑1 (MQ=255)


                                                             tctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTg                                    >  1:4403707/1‑101 (MQ=255)


                                                              ctGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTgg                                   <  1:55680/101‑1 (MQ=255)


                                                               tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGCCGAACCGCTCGCCCGCAGCCTTCTGGc                                  >  2:7079371/1‑101 (MQ=255)


                                                               tGGAGCCACATGACGATCCTGCAAAATGTCATGGAGGCCCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCCAACCGCTCGCCCGCAGCCTTCTGGc                                  >  1:457215/1‑101 (MQ=255)


                                                                ggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGct                                 >  2:2446946/1‑100 (MQ=255)


                                                                ggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGcc                                 <  2:1076324/101‑1 (MQ=255)


                                                                ggAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGcc                                 <  1:6562767/101‑1 (MQ=255)


                                                                 gAGCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCa                                <  2:6996335/101‑1 (MQ=255)


                                                                   gCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAg                              <  1:2885890/101‑1 (MQ=255)


                                                                   gCCACATGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAg                              <  2:3590003/101‑1 (MQ=255)


                                                                     cacaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAggc                            >  1:6537049/1‑100 (MQ=255)


                                                                     cacaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGt                            >  2:1965662/1‑101 (MQ=255)


                                                                      acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGCCGGTTCTGGGCCGCAAACCGGCCGAGCTCGAACCGCTCGCCCGGAGCCTTCTGGCCaatgcc                           >  2:797852/1‑97 (MQ=255)


                                                                      acaTGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTc                           >  2:3442441/1‑101 (MQ=255)


                                                                         tGACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGc                        >  2:6365396/1‑101 (MQ=255)


                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       >  2:4380524/1‑101 (MQ=255)


                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       >  2:3563230/1‑101 (MQ=255)


                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       >  2:3241393/1‑101 (MQ=255)


                                                                          gACGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCa                       >  2:1162875/1‑101 (MQ=255)


                                                                            caaTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     >  1:62396/3‑101 (MQ=255)


                                                                            cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     <  1:3057263/101‑1 (MQ=255)


                                                                            cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     >  1:5411450/1‑101 (MQ=255)


                                                                            cGATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATc                     <  2:157513/101‑1 (MQ=255)


                                                                             gATCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCg                    >  2:5628869/1‑101 (MQ=255)


                                                                              aTCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCgg                   <  2:4536895/101‑1 (MQ=255)


                                                                               tCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGc                  >  2:4318182/1‑101 (MQ=255)


                                                                               tCCTGCAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGc                  >  2:3892728/1‑101 (MQ=255)


                                                                                ccTGCAAAATGTCATGGAGGCTCCGGTGACGGTTCTGGGCCGCAATCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCg                 <  1:1010664/101‑1 (MQ=255)


                                                                                    cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa             >  2:1507496/1‑101 (MQ=255)


                                                                                    cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa             >  1:5675684/1‑101 (MQ=255)


                                                                                    cAAAATGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaa             >  1:1618653/1‑101 (MQ=255)


                                                                                     agaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag            <  2:4559834/99‑2 (MQ=255)


                                                                                     aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag            >  2:22914/1‑100 (MQ=255)


                                                                                     aaaaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACaag            <  2:4094751/101‑2 (MQ=255)


                                                                                        aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc         >  1:5331455/1‑101 (MQ=255)


                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        <  1:5947176/101‑1 (MQ=255)


                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        <  2:2725051/101‑1 (MQ=255)


                                                                                         tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg        >  1:2128184/1‑101 (MQ=255)


                                                                                          gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa       >  2:5197791/1‑101 (MQ=255)


                                                                                          gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa       >  1:3805590/1‑101 (MQ=255)


                                                                                          gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa       <  2:688842/101‑1 (MQ=255)


GACCGGGCGCAGGTAACGCGGTTTCGCACCAACCTGTCGATGGTGTTCCAGCAGTTCAACCTCTGGAGCCACATGACGATCCTGCAAAATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCT  >  NC_014034/2960401‑2960596

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG