Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,586 A→G 100% K152K (AAA→AAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5860A→G100.0% 1183.7 316K152K (AAA→AAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (166/150):  ref base (0/0):  total (166/150)

Only 100 of 325 total aligned reads displayed.

AATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960488‑2960683
                                                                                                  |                                                                                                 
aaTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGc                                                                                                 >  2:2220029/1‑101 (MQ=255)
  tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               <  1:1579227/101‑1 (MQ=255)
  tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               <  1:5947176/101‑1 (MQ=255)
  tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               >  1:2128184/1‑101 (MQ=255)
  tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               <  2:5137750/101‑1 (MQ=255)
   gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa                                                                                              <  2:1510908/101‑1 (MQ=255)
    tCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGAc                                                                                             <  1:6784546/101‑1 (MQ=255)
      agggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGGCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGGCGGCATCGGCGACAAGGCCGACGc                                                                                           <  2:5188147/99‑1 (MQ=255)
      aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  1:4385357/1‑101 (MQ=255)
          aggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTgg                                                                                       >  1:5506850/3‑101 (MQ=255)
          aggcgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTgg                                                                                       >  2:132840/3‑101 (MQ=255)
             cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                    <  2:1736895/101‑1 (MQ=255)
             cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                    <  2:4447071/101‑1 (MQ=255)
                cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgcg                                                                                 >  2:6876751/1‑101 (MQ=255)
                cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgcg                                                                                 >  2:823497/1‑101 (MQ=255)
                  gTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCa                                                                               >  1:3439610/1‑101 (MQ=255)
                  gTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCa                                                                               >  2:2648879/1‑101 (MQ=255)
                     aCGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                                                                            <  2:5289194/101‑1 (MQ=255)
                       ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                                                                          <  1:1784479/101‑1 (MQ=255)
                        gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                                                                         >  1:6661300/1‑101 (MQ=255)
                        gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                                                                         <  1:5195020/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCg                                                                        <  1:5006852/101‑1 (MQ=255)
                         ttCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCCCg                                                                        >  1:1385179/1‑101 (MQ=255)
                          tCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCgg                                                                       <  1:2325593/101‑1 (MQ=255)
                           cTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCggg                                                                      >  1:1831704/1‑101 (MQ=255)
                           cTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCggg                                                                      >  2:3601339/1‑101 (MQ=255)
                            tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  2:4897231/1‑101 (MQ=255)
                            tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  2:5054176/1‑101 (MQ=255)
                            tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  2:6911743/1‑101 (MQ=255)
                             gggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcg                                                                    >  2:6168155/1‑101 (MQ=255)
                             gggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCAGACGCCTGGCCCGCGCAGCTCTCGggcg                                                                    >  2:2096464/1‑101 (MQ=255)
                                 cGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCca                                                                <  2:3243932/101‑1 (MQ=255)
                                 cGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCca                                                                <  1:2736071/101‑1 (MQ=255)
                                  gCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcag                                                               <  1:2459146/101‑1 (MQ=255)
                                   cAAACCGGCCGAGGTCGGACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc                                                              >  1:4235915/1‑101 (MQ=255)
                                    aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             <  2:4028341/101‑1 (MQ=255)
                                     aaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagcag                                                            <  1:6259185/101‑1 (MQ=255)
                                       ccGTCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca                                                          <  1:1045644/101‑1 (MQ=255)
                                       ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca                                                          <  2:6581292/101‑1 (MQ=255)
                                       ccGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAgcagca                                                          <  2:4392381/101‑1 (MQ=255)
                                         ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        <  2:5348748/101‑1 (MQ=255)
                                         ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        >  1:2575198/1‑101 (MQ=255)
                                         ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        <  1:3041223/101‑1 (MQ=255)
                                         ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        <  2:3920266/101‑1 (MQ=255)
                                          gCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcg                                                       <  2:759477/101‑1 (MQ=255)
                                            cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg                                                     <  2:1964986/101‑1 (MQ=255)
                                             gaggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACgcgc                                                    >  2:6502342/4‑101 (MQ=255)
                                             gaggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACgcgc                                                    >  2:3709371/4‑101 (MQ=255)
                                              aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgcc                                                   <  2:5675394/99‑1 (MQ=255)
                                               ggTCGACCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccg                                                  <  2:2710441/100‑1 (MQ=255)
                                                 tCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCgccgcc                                                <  2:2584882/101‑1 (MQ=255)
                                                 tCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCgccgcc                                                <  2:546098/101‑1 (MQ=255)
                                                  cGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCa                                               >  1:4562058/1‑101 (MQ=255)
                                                  cGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCa                                               >  1:4044037/1‑101 (MQ=255)
                                                     aCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCg                                            <  2:2391882/101‑1 (MQ=255)
                                                     aCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCg                                            <  2:3532729/101‑1 (MQ=255)
                                                      ccGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGc                                           >  2:1642600/1‑101 (MQ=255)
                                                       cGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGcc                                          >  1:4844070/1‑101 (MQ=255)
                                                           cGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcgcg                                      >  1:5073388/1‑101 (MQ=255)
                                                             cccGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAGCGCGCCGCCATCGCCcgcgcg                                    >  2:1466015/1‑101 (MQ=255)
                                                                 cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                                >  1:3568591/1‑101 (MQ=255)
                                                                  cgCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtg                               <  1:5860856/100‑1 (MQ=255)
                                                                     cTTCTGGCCAGGGTCGG.ATCGGGCGCCAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatg                           <  2:5820146/101‑1 (MQ=25)
                                                                     cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                            <  1:3196870/101‑1 (MQ=255)
                                                                     cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                            >  1:4682455/1‑101 (MQ=255)
                                                                     cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                            >  1:6162197/1‑101 (MQ=255)
                                                                       tCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgc                          >  1:838797/1‑101 (MQ=255)
                                                                       tCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgc                          >  2:4013786/1‑101 (MQ=255)
                                                                        cTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgca                         <  1:2520717/101‑1 (MQ=255)
                                                                         tGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAg                        <  1:7050147/101‑1 (MQ=255)
                                                                         tGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAg                        >  1:7127330/1‑101 (MQ=255)
                                                                          ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       <  2:4837955/101‑1 (MQ=255)
                                                                           gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                      <  1:987119/101‑1 (MQ=255)
                                                                           gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                      <  2:6320218/101‑1 (MQ=255)
                                                                           gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                      <  1:3892891/101‑1 (MQ=255)
                                                                             cAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    <  1:973965/101‑1 (MQ=255)
                                                                              aaGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCa                   >  2:1793301/1‑101 (MQ=255)
                                                                               aGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAg                  >  2:6216730/1‑101 (MQ=255)
                                                                               aGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAg                  >  1:1938434/1‑101 (MQ=255)
                                                                                ggTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAgg                 <  1:1162923/101‑1 (MQ=255)
                                                                                ggTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAgg                 >  2:3099587/1‑101 (MQ=255)
                                                                                 gTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGgc                <  2:60126/101‑1 (MQ=255)
                                                                                 gTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGgc                >  2:117490/1‑101 (MQ=255)
                                                                                    ggCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTAGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCt             >  2:4644542/1‑101 (MQ=255)
                                                                                     gCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtt            >  2:2448198/1‑101 (MQ=255)
                                                                                     gCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtt            >  1:2646001/1‑101 (MQ=255)
                                                                                      cATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCttt           >  1:3760119/1‑101 (MQ=255)
                                                                                      cATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCttt           >  2:7031221/1‑101 (MQ=255)
                                                                                      cATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCCCCCGCGCGCTCTGCATGCAGCCGCAGGCGCttt           <  1:6365720/101‑1 (MQ=255)
                                                                                       aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt          <  2:5862266/101‑1 (MQ=255)
                                                                                       aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt          <  2:6613323/101‑1 (MQ=255)
                                                                                         cGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGt        <  1:6488275/101‑1 (MQ=255)
                                                                                          ggCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGtt       <  2:2787173/101‑1 (MQ=255)
                                                                                           gCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTc      <  1:5692172/101‑1 (MQ=255)
                                                                                            cGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcg     >  1:287368/1‑101 (MQ=255)
                                                                                             gACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgc    >  2:1266641/1‑100 (MQ=255)
                                                                                             gACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcga    <  2:4190243/101‑1 (MQ=255)
                                                                                             gACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcga    >  1:1297189/1‑101 (MQ=255)
                                                                                              aCAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgac   <  1:121268/101‑1 (MQ=255)
                                                                                               cAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgacg  <  2:6360230/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960488‑2960683

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG