Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,962,711 A→G 100% K21E (AAG→GAG)  hisM → polar amino acid ABC transporter permease HisM

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,962,7110A→G100.0% 1312.0 352K21E (AAG→GAG) hisMpolar amino acid ABC transporter permease HisM
Reads supporting (aligned to +/- strand):  new base (164/188):  ref base (0/0):  total (164/188)

Only 100 of 363 total aligned reads displayed.

CGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962614‑2962808
                                                                                                 |                                                                                                 
cgacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgc                                                                                                <  1:4200729/101‑1 (MQ=255)
 gacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgcg                                                                                               >  1:6102543/1‑101 (MQ=255)
      tGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgc                                                                                          <  2:7101675/101‑1 (MQ=255)
         cgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgctgc                                                                                       >  1:4645735/1‑101 (MQ=255)
         cgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgctgc                                                                                       <  2:6163182/101‑1 (MQ=255)
         cgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgctgc                                                                                       >  2:4937233/1‑101 (MQ=255)
          gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGGACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGGGCTGcc                                                                                      >  1:1778949/1‑101 (MQ=255)
             cgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCcgcg                                                                                   <  2:4679952/101‑1 (MQ=255)
               aaGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCa                                                                                 >  2:5717393/1‑101 (MQ=255)
                    cagcGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGAc                                                                            <  2:4222881/98‑1 (MQ=255)
                    cAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGAc                                                                            <  1:798306/101‑1 (MQ=255)
                     aaCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           >  2:1791675/1‑101 (MQ=255)
                     aaCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           >  2:2088714/1‑101 (MQ=255)
                     aaCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           >  1:4489242/1‑101 (MQ=255)
                     aaCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           <  2:6204592/101‑1 (MQ=255)
                      aCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACtt                                                                          <  1:2569690/101‑1 (MQ=255)
                       cGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTc                                                                         >  1:5947638/1‑101 (MQ=255)
                        ggAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCa                                                                        <  1:6234730/101‑1 (MQ=255)
                         gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       <  1:892876/101‑1 (MQ=255)
                          aaGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACg                                                                      <  2:4737101/101‑1 (MQ=255)
                          aaGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACg                                                                      >  1:4281971/1‑101 (MQ=255)
                             gCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGctc                                                                   <  1:4320551/101‑1 (MQ=255)
                                  ggCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCa                                                              >  1:5111582/1‑101 (MQ=255)
                                   gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             <  2:3049580/101‑1 (MQ=255)
                                    cATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATg                                                            >  2:3774704/1‑101 (MQ=255)
                                    cATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATg                                                            >  1:6707313/1‑101 (MQ=255)
                                    cATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATg                                                            >  1:1386252/1‑101 (MQ=255)
                                    cATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATg                                                            >  2:358259/1‑101 (MQ=255)
                                     aTGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGa                                                           <  2:2775104/101‑1 (MQ=255)
                                        aGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAAtt                                                        <  2:1458432/101‑1 (MQ=255)
                                         gCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTc                                                       <  1:4937485/101‑1 (MQ=255)
                                          ccagaGCCGCTCGCAGATCATCGCGGATTTCCCCTCCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      <  2:1291190/97‑1 (MQ=255)
                                          cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      >  2:6384719/1‑101 (MQ=255)
                                          cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      <  1:5475947/101‑1 (MQ=255)
                                          cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      >  2:77691/1‑101 (MQ=255)
                                          cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      >  2:3011268/1‑101 (MQ=255)
                                           gagaGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGGTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAc                                                     <  2:2243012/98‑1 (MQ=255)
                                            aCAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAcc                                                    <  2:5408870/101‑1 (MQ=255)
                                              accTGCACGCAGATCATCGCGGATTCCGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCt                                                  <  1:2273508/99‑1 (MQ=255)
                                              aGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTAAGGCGAGAGCGTGCTGCCGCGCAGTGACTTCACGCTCCGCCATGGATTCAcccc                                                  >  2:4361882/1‑100 (MQ=255)
                                               gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 >  2:20340/1‑101 (MQ=255)
                                               gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 <  1:3299362/101‑1 (MQ=255)
                                               gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 <  1:5839653/101‑1 (MQ=255)
                                               gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 <  1:4416512/101‑1 (MQ=255)
                                                cTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGa                                                >  1:2144590/1‑101 (MQ=255)
                                                cTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGa                                                <  2:2343771/101‑1 (MQ=255)
                                                  gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              <  2:555297/101‑1 (MQ=255)
                                                   cACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             <  2:544901/101‑1 (MQ=255)
                                                    aCGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCgg                                            <  1:4744268/101‑1 (MQ=255)
                                                    aCGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCgg                                            >  1:565840/1‑101 (MQ=255)
                                                     cGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGc                                           <  1:6980209/101‑1 (MQ=255)
                                                     cGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGc                                           >  1:522651/1‑101 (MQ=255)
                                                      gCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCt                                          <  2:6820667/101‑1 (MQ=255)
                                                       cAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTc                                         >  2:2832881/1‑101 (MQ=255)
                                                           tcatcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgggg                                     >  1:3234421/1‑101 (MQ=255)
                                                            catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    <  1:2863550/101‑1 (MQ=255)
                                                            catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    >  2:1022908/1‑101 (MQ=255)
                                                            caacgcgGATTCCGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    <  1:499166/98‑1 (MQ=255)
                                                              tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  <  1:3612448/101‑1 (MQ=255)
                                                              tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  <  2:587639/101‑1 (MQ=255)
                                                                gcgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGAt                                >  1:2065799/1‑101 (MQ=255)
                                                                 ccgATTCCCCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATc                               <  2:3278580/99‑1 (MQ=255)
                                                                   gATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTg                             >  2:3637968/1‑101 (MQ=255)
                                                                    aTTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGGTCTgg                            >  1:100269/1‑101 (MQ=255)
                                                                    aTTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTgg                            <  1:5037238/101‑1 (MQ=255)
                                                                    aTTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTgg                            <  2:6634521/101‑1 (MQ=255)
                                                                      tACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGaa                          <  1:2421301/101‑1 (MQ=255)
                                                                      tACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGaa                          <  1:5868559/101‑1 (MQ=255)
                                                                         gCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGt                       <  1:1487488/101‑1 (MQ=255)
                                                                          ccTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTc                      <  1:3905283/101‑1 (MQ=255)
                                                                          ccTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCCGGGCTGATCTGGAACggc                      >  2:2979181/1‑99 (MQ=255)
                                                                           cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCt                     >  1:7079956/1‑101 (MQ=255)
                                                                           cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCt                     <  2:4822993/101‑1 (MQ=255)
                                                                             tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  2:4396768/101‑1 (MQ=255)
                                                                              ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  1:4699238/101‑1 (MQ=255)
                                                                              ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  <  2:369279/101‑1 (MQ=255)
                                                                               cgctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAttt                 <  1:1313036/101‑1 (MQ=255)
                                                                               cgctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAttt                 <  1:1070320/101‑1 (MQ=255)
                                                                                gctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGGACGTCTATTTc                >  2:4884124/1‑101 (MQ=255)
                                                                                 ctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCg               <  1:6594189/101‑1 (MQ=255)
                                                                                  tcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCgg              <  2:5343437/101‑1 (MQ=255)
                                                                                    gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAAGTCTATTTCGGCa            >  2:5980174/1‑101 (MQ=255)
                                                                                     ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGGACGTCTATTTCGGCAc           >  1:3726858/1‑101 (MQ=255)
                                                                                     ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  2:1381191/101‑1 (MQ=255)
                                                                                     ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  2:3464496/101‑1 (MQ=255)
                                                                                     ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  2:380264/101‑1 (MQ=255)
                                                                                     ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  2:565828/101‑1 (MQ=255)
                                                                                     ctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCAc           <  1:2347224/101‑1 (MQ=255)
                                                                                      tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          >  1:1206762/1‑101 (MQ=255)
                                                                                      tGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACg          >  2:2507611/1‑101 (MQ=255)
                                                                                       gggCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGc         >  1:4013285/1‑101 (MQ=255)
                                                                                       gggCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGc         >  2:2566302/1‑101 (MQ=255)
                                                                                         gCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTg       <  1:5717695/101‑1 (MQ=255)
                                                                                          cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTgg      >  1:378151/1‑101 (MQ=255)
                                                                                          cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTgg      <  2:6303000/101‑1 (MQ=255)
                                                                                           tATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgc     >  2:601634/1‑101 (MQ=255)
                                                                                            aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgcg    >  1:2744652/1‑101 (MQ=255)
                                                                                            aTGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAAGTCTATTTCGGCACGCTGgcg    >  2:6921799/1‑101 (MQ=255)
                                                                                              ggCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGGACGCTGGCGCt  >  2:2911710/1‑101 (MQ=255)
                                                                                              ggCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCt  >  1:1690194/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
CGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962614‑2962808

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG