Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 297,560 A→G 100% K12E (AAG→GAG)  RCAP_rcc00260 → alanine racemase domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034297,5600A→G100.0% 1296.5 345K12E (AAG→GAG) RCAP_rcc00260alanine racemase domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (199/146):  ref base (0/0):  total (199/146)

Only 100 of 359 total aligned reads displayed.

GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657
                                                                                                   |                                                                                                 
gggCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAg                                                                                                 >  1:4549673/1‑101 (MQ=255)
 ggCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAgg                                                                                                >  2:2888619/1‑101 (MQ=255)
  gCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGc                                                                                               <  1:5428853/101‑1 (MQ=255)
   ccTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCg                                                                                              <  2:5282234/101‑1 (MQ=255)
      tGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAcc                                                                                           <  1:830861/101‑1 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAccc                                                                                          <  2:1228941/101‑1 (MQ=255)
            cgctcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggc                                                                                      >  1:6653623/4‑101 (MQ=255)
            cgctcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggc                                                                                      >  2:6609581/4‑101 (MQ=255)
             gctcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggcc                                                                                     >  1:2530023/3‑101 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   <  2:2038629/101‑1 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   >  1:6644511/1‑101 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   >  2:3689702/1‑101 (MQ=255)
               tcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgc                                                                                   <  1:4757761/101‑1 (MQ=255)
                cttttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcg                                                                                  >  1:265962/1‑101 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               >  2:481795/1‑101 (MQ=255)
                    tcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggcc                                                                              <  2:275958/101‑1 (MQ=255)
                    tcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggcc                                                                              <  2:2557217/101‑1 (MQ=255)
                     cttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             >  1:3442810/1‑101 (MQ=255)
                     cttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             >  1:2864772/1‑101 (MQ=255)
                     cttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             >  1:1515295/1‑101 (MQ=255)
                     cttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             >  1:4360671/1‑101 (MQ=255)
                      ttttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgg                                                                            <  2:957364/101‑1 (MQ=255)
                        ttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCAATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgggc                                                                          >  2:6478382/1‑101 (MQ=255)
                          ttGCCGCCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGggccgggccg                                                                        <  2:6452781/101‑1 (MQ=255)
                          ttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGggccgggccg                                                                        >  2:6906535/1‑101 (MQ=255)
                           tGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGa                                                                       >  1:6398398/1‑101 (MQ=255)
                            gCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAg                                                                      >  2:2182122/1‑101 (MQ=255)
                            gCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAg                                                                      <  2:238234/101‑1 (MQ=255)
                             ccACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAgg                                                                     >  2:4714336/1‑101 (MQ=255)
                                  ttGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCg                                                                >  1:7029542/1‑101 (MQ=255)
                                  ttGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCg                                                                <  1:2960152/101‑1 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  2:6277326/1‑101 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGCGGCCACCCGGGCCCCGGGCCCGGCCGCGGGGGCggc                                                              >  2:2301642/1‑100 (MQ=255)
                                    gtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGTGCCGGGCCGAGGGGGCggg                                                              >  2:1753712/1‑100 (MQ=255)
                                     tgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTg                                                             <  1:5224268/101‑1 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGc                                                            <  1:5496401/101‑1 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGc                                                            >  1:6031264/1‑101 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGc                                                            >  1:1243588/1‑101 (MQ=255)
                                       cTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCg                                                           <  1:2594386/101‑1 (MQ=255)
                                       cTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCg                                                           <  2:4174936/101‑1 (MQ=255)
                                        tCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCgg                                                          >  1:3665981/1‑101 (MQ=255)
                                           aTTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGCGGGGGCGGTGCGGCTg                                                       >  2:3189588/1‑101 (MQ=255)
                                           aTTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTg                                                       <  1:2149975/101‑1 (MQ=255)
                                            ttGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGCCCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGa                                                      <  1:4944149/101‑1 (MQ=255)
                                             tGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGAt                                                     >  1:3012644/1‑101 (MQ=255)
                                              gcgcCGCAAAGGAGATCNCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGAtn                                                    <  2:1987972/101‑2 (MQ=255)
                                              gcgcCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGAtg                                                    >  2:4309901/1‑100 (MQ=255)
                                              gcgcCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATc                                                    <  1:4437402/101‑1 (MQ=255)
                                                gccgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGc                                                  >  2:5749150/1‑101 (MQ=255)
                                                 ccgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGcc                                                 <  2:5620503/101‑1 (MQ=255)
                                                   gcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgt                                               <  2:3413218/101‑1 (MQ=255)
                                                   gcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgg                                               >  1:1960028/1‑100 (MQ=255)
                                                    cAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtg                                              <  2:6090954/101‑1 (MQ=255)
                                                    cAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCGGATCGCCgtg                                              >  1:3279931/1‑101 (MQ=255)
                                                     aaaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtgt                                             >  2:760569/1‑101 (MQ=255)
                                                     aaaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtgt                                             >  2:1345991/1‑101 (MQ=255)
                                                      aaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTc                                            >  2:5235372/1‑101 (MQ=255)
                                                      aaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTc                                            >  2:3525620/1‑101 (MQ=255)
                                                       aGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCg                                           <  1:4596598/101‑1 (MQ=255)
                                                        ggAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGa                                          >  1:3933299/1‑101 (MQ=255)
                                                        ggAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGa                                          <  2:6217332/101‑1 (MQ=255)
                                                        ggAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGa                                          <  2:5687180/101‑1 (MQ=255)
                                                         gagaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGaa                                         <  2:6719500/101‑1 (MQ=255)
                                                             tCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTg                                     <  2:4482730/101‑1 (MQ=255)
                                                              cGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    <  2:5434912/101‑1 (MQ=255)
                                                              cGCCAGGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    <  2:6653281/101‑1 (MQ=255)
                                                                ccATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAg                                  >  2:4403667/1‑101 (MQ=255)
                                                                ccATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGAGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGGTGATCGGCGTGTCGAAgggggag                                  >  2:7059456/1‑96 (MQ=255)
                                                                 cATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgc                                 <  1:5724547/101‑1 (MQ=255)
                                                                 cATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgc                                 >  1:4820713/1‑101 (MQ=255)
                                                                  aTGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgcc                                <  2:3605111/101‑1 (MQ=255)
                                                                  aTGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgcc                                <  1:4332267/101‑1 (MQ=255)
                                                                  aTGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgcc                                <  1:1086470/101‑1 (MQ=255)
                                                                    gggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccgc                              <  1:4011732/101‑1 (MQ=255)
                                                                     ggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccgcc                             >  2:1101559/1‑101 (MQ=255)
                                                                     ggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGGGCAgccgcc                             >  1:5331427/1‑101 (MQ=255)
                                                                       aTTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCg                           <  2:1643515/101‑1 (MQ=255)
                                                                        ttGCAGGAGCTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGa                          <  2:6811045/101‑1 (MQ=255)
                                                                        ttGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGa                          <  1:6884753/101‑1 (MQ=255)
                                                                          gCAGGGGATTCAGACGCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTAGAAGGTGCAGCCGCCCGAGc                        >  1:5628799/1‑101 (MQ=255)
                                                                          gCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGc                        >  1:1572981/1‑101 (MQ=255)
                                                                          gCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGc                        >  2:5926794/1‑101 (MQ=255)
                                                                              gagaTTCAGCCCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGt                    <  2:5338281/101‑1 (MQ=255)
                                                                                  ttCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTg                <  1:5777024/101‑1 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGCTCGCCGGGTCGAAGGGGCAGCCGCCCGAGCGGGGGCTGGc              >  1:2257688/1‑101 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGc              <  2:2520120/101‑1 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGc              >  2:1027274/1‑101 (MQ=255)
                                                                                     aagcccGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCg             <  2:5954629/98‑1 (MQ=255)
                                                                                      gACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCgg            >  1:5937577/1‑101 (MQ=255)
                                                                                      gACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCgg            >  1:3569779/1‑101 (MQ=255)
                                                                                       gcccGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGt           <  2:3012531/100‑1 (MQ=255)
                                                                                        cccGGATGGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTg          <  1:2029588/101‑1 (MQ=255)
                                                                                          cGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCt        >  1:6158354/1‑101 (MQ=255)
                                                                                          cGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGGGCTGGCGGTGcc        >  2:4119019/1‑100 (MQ=255)
                                                                                           ggATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTc       <  1:4116741/101‑1 (MQ=255)
                                                                                           ggATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGGGCTc       >  2:5007024/1‑101 (MQ=255)
                                                                                            gATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCg      <  1:6234095/101‑1 (MQ=255)
                                                                                            gATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCg      <  2:3900235/101‑1 (MQ=255)
                                                                                             aTTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGGGGGGGCGGTGCGGCTGATCGCCGTGGCGAAGGTGCAGCCGCCCGTGCGGGCGCTGGCGGTGCTCGa     >  1:7101531/1‑101 (MQ=255)
                                                                                                gCGGAGGCGGCCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCa  <  2:3265360/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG