BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,011,287	A→T	78.9%	intergenic (+86/+56)	purK → / ← RCAP_rcc02822	phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,011,287	0	A→T	78.9%	217.8 ‑0.1 0.0	298	intergenic (+86/+56)	purK/RCAP_rcc02822	phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Reads supporting (aligned to +/- strand): new base (142/93): ref base (37/26): total (179/119)
Fisher's exact test strand distribution p-value = 8.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.


TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379


ttCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCgg                                                                                        <  1:1427993/101‑1 (MQ=255)


 tctcTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCggg                                                                                       >  1:5854107/1‑101 (MQ=255)


 tctcTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCggg                                                                                       >  2:3500304/1‑101 (MQ=255)


  ctctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCgggg                                                                                      >  2:4408639/1‑101 (MQ=255)


  ctctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCgggg                                                                                      >  1:5906328/1‑101 (MQ=255)


  ctctGACCGCGGAAGCGGGGGCTGTCTGGCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAGACAACGCCCTTTCACCTTGGGA.C.AAATATCCCgggg                                                                                      >  1:5067481/1‑101 (MQ=25)


   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCggggg                                                                                     >  2:1459681/1‑101 (MQ=255)


    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCgggggg                                                                                    >  2:5831472/1‑101 (MQ=255)


      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGgag                                                                                  >  1:6360118/1‑101 (MQ=255)


      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGgag                                                                                  >  2:2386341/1‑101 (MQ=255)


       aCCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGgaga                                                                                 <  2:2988348/101‑1 (MQ=255)


       aCCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGgagc                                                                                 >  2:5400014/1‑100 (MQ=255)


        ccGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGAc                                                                                <  2:6668370/101‑1 (MQ=255)


        ccGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGAc                                                                                <  1:5772732/101‑1 (MQ=255)


         cgcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACt                                                                               >  2:1335486/1‑101 (MQ=255)


         cgcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACt                                                                               >  2:6738075/1‑101 (MQ=255)


          gcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACtt                                                                              <  2:2693868/101‑1 (MQ=255)


          gcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACtt                                                                              <  1:864347/101‑1 (MQ=255)


             gCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGcc                                                                           <  1:271922/101‑1 (MQ=255)


              cAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCg                                                                          <  1:4947289/101‑1 (MQ=255)


               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGc                                                                         >  1:3268831/1‑101 (MQ=255)


               aGCGGGGGCTGTCTGCCCCCGCACCCCCGACGATATTTCCGCCAAGGCGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGc                                                                         >  2:5183278/1‑101 (MQ=38)


                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAg                                                                       >  2:4566051/1‑101 (MQ=255)


                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAgg                                                                      <  1:3375802/101‑1 (MQ=255)


                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                      >  2:4321963/1‑101 (MQ=255)


                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                      >  2:2094142/1‑101 (MQ=255)


                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                      >  1:211740/1‑101 (MQ=255)


                    gggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCa                                                                    <  2:1493055/101‑1 (MQ=255)


                     ggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCaa                                                                   <  1:1553222/101‑1 (MQ=255)


                       cTGTCTGCCCCCGCACCCCCGACGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAaga                                                                 <  2:708850/101‑1 (MQ=39)


                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                                <  2:1895628/101‑1 (MQ=255)


                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                                >  2:1225197/1‑101 (MQ=255)


                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                               >  1:4534572/1‑101 (MQ=255)


                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                               >  2:3783308/1‑101 (MQ=255)


                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                               >  2:4797655/1‑101 (MQ=255)


                          tCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAggg                                                              >  2:5518327/1‑101 (MQ=255)


                           cTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggg                                                             >  1:2956701/1‑101 (MQ=255)


                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggggg                                                           >  2:6156978/1‑101 (MQ=255)


                              cccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                          <  2:6341558/101‑1 (MQ=255)


                                 ccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.c                                                      <  2:3376989/101‑1 (MQ=255)


                                 ccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.c                                                      <  2:5603930/101‑1 (MQ=255)


                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.cgc                                                    >  2:3272288/1‑101 (MQ=255)


                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.cgc                                                    >  2:1408883/1‑101 (MQ=255)


                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.cgc                                                    >  1:3621578/1‑101 (MQ=255)


                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.cgc                                                    >  1:4921411/1‑101 (MQ=255)


                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.cgc                                                    >  1:1012488/1‑101 (MQ=255)


                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTATCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg.cgc                                                    <  1:5121435/101‑1 (MQ=38)


                                    cACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGcc                                                   >  1:5526606/1‑101 (MQ=255)


                                     aCCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGccc                                                  >  1:1926356/1‑101 (MQ=255)


                                       ccccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGccccc                                                <  1:3052281/101‑1 (MQ=255)


                                        cccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGcccccc                                               >  1:4575846/1‑101 (MQ=255)


                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCt                                              <  1:535134/101‑1 (MQ=255)


                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCt                                              <  1:4408861/101‑1 (MQ=255)


                                          cGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTg                                             >  1:7057155/1‑101 (MQ=255)


                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGc                                            >  2:280841/1‑101 (MQ=255)


                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGc                                            >  1:91902/1‑101 (MQ=255)


                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGc                                            <  2:1787102/101‑1 (MQ=255)


                                            aGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGcc                                           >  2:2052390/1‑101 (MQ=255)


                                             ggATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGccc                                          <  2:3185678/101‑1 (MQ=255)


                                             ggATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGccc                                          >  2:2902989/1‑101 (MQ=255)


                                               atatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCgg                                        >  2:3857422/1‑101 (MQ=255)


                                                 atTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCt                                      >  1:1179860/1‑101 (MQ=255)


                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCa                                    <  1:561462/101‑1 (MQ=255)


                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCa                                    <  1:4049032/101‑1 (MQ=255)


                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCa                                    >  2:3048490/1‑101 (MQ=255)


                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGCGTGACTTGCCGCAGGCAAGAGGCGGGAAC.CGCCCCCCTGCCCGGCTCa                                    >  2:501935/1‑101 (MQ=255)


                                                    tCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCAc                                   <  2:4980245/101‑1 (MQ=255)


                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACgg                                 >  1:5450442/1‑101 (MQ=255)


                                                       gCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGc                                >  1:6761111/1‑101 (MQ=255)


                                                        ccAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGcg                               >  2:761868/1‑100 (MQ=255)


                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAg                              >  2:4146438/1‑101 (MQ=255)


                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAg                              >  1:6901999/1‑101 (MQ=255)


                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAgg                             <  2:568586/101‑1 (MQ=255)


                                                             gTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGcc                          <  1:6666323/101‑1 (MQ=255)


                                                             gTGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGcc                          <  2:1019249/101‑1 (MQ=255)


                                                              tGAAAACAACGCGCTTTCACCTTGGGACC.AATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGCGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         >  1:6278598/1‑101 (MQ=37)


                                                              tGAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCGG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         >  1:2572981/1‑101 (MQ=37)


                                                              tGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         <  2:4048138/101‑1 (MQ=255)


                                                              tGAAAACAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCa                         <  2:5067221/101‑1 (MQ=255)


                                                               gAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGcccg                        >  1:3129475/1‑99 (MQ=25)


                                                               gAAAACAACGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAg                        >  1:2655737/1‑101 (MQ=255)


                                                                aaaaCAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGCCTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGc                       >  1:346474/1‑101 (MQ=37)


                                                                 aaaCAACGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                      >  2:923753/1‑101 (MQ=255)


                                                                  aacaacGCCCTTTCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCg                     >  2:3588390/1‑101 (MQ=255)


                                                                  aacaacGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGccc                     >  2:3648085/1‑100 (MQ=255)


                                                                      acGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGc                 <  1:6129232/101‑1 (MQ=255)


                                                                      acGCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGc                 <  1:1776502/101‑1 (MQ=255)


                                                                        gCCCTTTCACCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa               <  1:4797898/101‑1 (MQ=255)


                                                                         cccTTTCCCCTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAg              <  2:3735174/101‑1 (MQ=37)


                                                                            tttCACCTTGGGA.CAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGcc           >  2:861596/1‑101 (MQ=255)


                                                                            cttCCCCTTGGGA.A.AAAATTCCCGGGGGGAAGCTTTGCGGTGGCAAGGGGGGGGCGGCCCCCCCCCTGCCCGCCCCTCGGCAGGGCCAGCCGCTGCAAGGc            <  2:1622141/100‑1 (MQ=255)


                                                                              tccccTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgc         <  2:7009653/98‑1 (MQ=37)


                                                                                 ccTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcc      >  1:6499313/1‑101 (MQ=255)


                                                                                  cTTGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGccc     >  1:1690/1‑101 (MQ=255)


                                                                                   ttGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc    >  1:221086/1‑101 (MQ=255)


                                                                                   ttGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAAGCCGCGcccc    >  2:2201784/1‑101 (MQ=37)


                                                                                   ttGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCCGCAAGGCCGCGcccc    >  2:1948004/1‑101 (MQ=37)


                                                                                   ttGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGACNCTGCAAGGGCGCGcccc    >  2:682955/1‑101 (MQ=25)


                                                                                    tGGGA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa   >  1:4329317/1‑101 (MQ=255)


                                                                                     gggA.C.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAg  >  2:2820889/1‑101 (MQ=255)


TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGA.C.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAG.CGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG